目的分析镜像疗法近20年的研究现状和热点。方法以Web of Science中SCI-E为文献来源,检索1998年至2019年镜像疗法相关研究,采用CiteSpace分析作者、国家/机构的合作网络图谱;对关键词进行共现分析、聚类分析、时间演化分析和Burst分析;...目的分析镜像疗法近20年的研究现状和热点。方法以Web of Science中SCI-E为文献来源,检索1998年至2019年镜像疗法相关研究,采用CiteSpace分析作者、国家/机构的合作网络图谱;对关键词进行共现分析、聚类分析、时间演化分析和Burst分析;以被引文献、被引期刊为节点绘制共被引图谱,并对被引文献进行Burst分析。结果共纳入文献435篇。高产作者以Mosaley G L、Tsao J W为代表;高产国家为美国、德国、英国;高产机构为海德堡大学,南澳大学。预测幻肢痛、复杂区域疼痛综合征、脑卒中后运动功能尤其是上肢功能的改善,以及增强现实等主题将成为镜像疗法未来一段时间内的研究热点结论镜像疗法这一研究领域仍处于起步阶段,发展较为迅速但影响力不足。展开更多
Objective:To investigate whether the antihypertensive mechanism of electroacupuncture(EA)is associated with attenuating phenotype transformation of vascular smooth muscle cells(VSMCs)via phosphoinositide3-kinase(PI3K)...Objective:To investigate whether the antihypertensive mechanism of electroacupuncture(EA)is associated with attenuating phenotype transformation of vascular smooth muscle cells(VSMCs)via phosphoinositide3-kinase(PI3K)/protein kinase B(Akt)and mitogen-activated protein kinase(MAPK)signaling pathways.Methods:Eight Wistar-ktoyo(WKY)rats were set as normal blood pressure group(normal group).A total of 32 spontaneous hypertensive rats(SHRs)were randomly divided into 4 groups using random number tables:a model group,an EA group,an EA+PI3K antagonist group(EA+P group),and an EA+p38 MAPK agonist+extracellular signal-regulated kinase(ERK)agonist group(EA+M group)(n=8/group).SHRs in EA group,EA+P group and EA+M group received EA treatment 5 sessions per week for continuous 4 weeks,while rats in the normal and model groups were bundled in same condition.The systolic blood pressure(SBP),diastolic blood pressure(DBP),and mean arterial pressure(MAP)of each rat was measured at 0 week and the 4th week.After 4-week intervention,thoracic aorta was collected for hematoxylin-eosin(HE)staining,immunohistochemistry[the contractile markersα-smooth muscle actin(α-SMA)and calponin and the synthetic marker osteopontin(OPN)]and Western blot[α-SMA,calponin,OPN,PI3K,phosphorylated-Akt(p-Akt),Akt,p-p42/44 ERK,total p42/44 ERK,p-p38 MAPK and total p38 MAPK].Results:EA significantly reduced SBP,DBP and MAP(P<0.01).HE staining showed that the wall thickness of thoracic aorta in EA group was significantly decreased(P<0.01).From results of immunohistochemistry and Western blot,EA increased the expression ofα-SMA and calponin,and decreased the expression of OPN(P<0.01).In addition,the expression of PI3K and p-Akt increased(P<0.01),while the expression of p-p42/44 ERK and p-p38 MAPK decreased in EA group(P<0.01).However,these effects were reversed by PI3K antagonist,p38 MAPK agonist and ERK agonist.Conclusions:EA was an effective treatment for BP management.The antihypertensive effect of EA may be related with inhibition of phenotypic transformation of VSMCs,in which the activation of PI3K/Akt and the repression of MAPK pathway were involved.展开更多
文摘目的分析镜像疗法近20年的研究现状和热点。方法以Web of Science中SCI-E为文献来源,检索1998年至2019年镜像疗法相关研究,采用CiteSpace分析作者、国家/机构的合作网络图谱;对关键词进行共现分析、聚类分析、时间演化分析和Burst分析;以被引文献、被引期刊为节点绘制共被引图谱,并对被引文献进行Burst分析。结果共纳入文献435篇。高产作者以Mosaley G L、Tsao J W为代表;高产国家为美国、德国、英国;高产机构为海德堡大学,南澳大学。预测幻肢痛、复杂区域疼痛综合征、脑卒中后运动功能尤其是上肢功能的改善,以及增强现实等主题将成为镜像疗法未来一段时间内的研究热点结论镜像疗法这一研究领域仍处于起步阶段,发展较为迅速但影响力不足。
基金Supported by the National Natural Science Foundation of China(Nos.81704137,82074516)Sichuan Science and Technology Department(No.2019YJ0331)。
文摘Objective:To investigate whether the antihypertensive mechanism of electroacupuncture(EA)is associated with attenuating phenotype transformation of vascular smooth muscle cells(VSMCs)via phosphoinositide3-kinase(PI3K)/protein kinase B(Akt)and mitogen-activated protein kinase(MAPK)signaling pathways.Methods:Eight Wistar-ktoyo(WKY)rats were set as normal blood pressure group(normal group).A total of 32 spontaneous hypertensive rats(SHRs)were randomly divided into 4 groups using random number tables:a model group,an EA group,an EA+PI3K antagonist group(EA+P group),and an EA+p38 MAPK agonist+extracellular signal-regulated kinase(ERK)agonist group(EA+M group)(n=8/group).SHRs in EA group,EA+P group and EA+M group received EA treatment 5 sessions per week for continuous 4 weeks,while rats in the normal and model groups were bundled in same condition.The systolic blood pressure(SBP),diastolic blood pressure(DBP),and mean arterial pressure(MAP)of each rat was measured at 0 week and the 4th week.After 4-week intervention,thoracic aorta was collected for hematoxylin-eosin(HE)staining,immunohistochemistry[the contractile markersα-smooth muscle actin(α-SMA)and calponin and the synthetic marker osteopontin(OPN)]and Western blot[α-SMA,calponin,OPN,PI3K,phosphorylated-Akt(p-Akt),Akt,p-p42/44 ERK,total p42/44 ERK,p-p38 MAPK and total p38 MAPK].Results:EA significantly reduced SBP,DBP and MAP(P<0.01).HE staining showed that the wall thickness of thoracic aorta in EA group was significantly decreased(P<0.01).From results of immunohistochemistry and Western blot,EA increased the expression ofα-SMA and calponin,and decreased the expression of OPN(P<0.01).In addition,the expression of PI3K and p-Akt increased(P<0.01),while the expression of p-p42/44 ERK and p-p38 MAPK decreased in EA group(P<0.01).However,these effects were reversed by PI3K antagonist,p38 MAPK agonist and ERK agonist.Conclusions:EA was an effective treatment for BP management.The antihypertensive effect of EA may be related with inhibition of phenotypic transformation of VSMCs,in which the activation of PI3K/Akt and the repression of MAPK pathway were involved.
