Traditionally,Tripterygium hypoglaucum(Levl.)Hutch(THH)are widely used in Chinese folk to treat rheumatoid arthritis(RA).This study aimed to investigate whether the anti-RA effect of THH is related with the gut microb...Traditionally,Tripterygium hypoglaucum(Levl.)Hutch(THH)are widely used in Chinese folk to treat rheumatoid arthritis(RA).This study aimed to investigate whether the anti-RA effect of THH is related with the gut microbiota.The main components of prepared THH extract were identified by HPLC-MS.C57BL/6 mice with adjuvant-induced arthritis(AIA)were treated with THH extract by gavage for one month.THH extract significantly alleviated swollen ankle,joint cavity exudation,and articular cartilage destruction in AIA mice.The mRNA and protein levels of inflammatory mediators in muscles and plasma indicated that THH extract attenuated inflammatory responses in the joint by blocking TLR4/MyD88/MAPK signaling pathways.THH extract remarkably restored the dysbiosis of the gut microbiota in AIA mice,featuring the increases of Bifidobacterium,Akkermansia,and Lactobacillus and the decreases of Butyricimonas,Parabacteroides,and Anaeroplasma.Furthermore,the altered bacteria were closely correlated with physiological indices and drove metabolic changes of the intestinal microbiota.In addition,antibiotic-induced pseudo germ-free mice were employed to verify the role of the intestinal flora.Strikingly,THH treatment failed to ameliorate the arthritis symptoms and signaling pathways in pseudo germ-free mice,which validates the indispensable role of the intestinal flora.For the first time,we demonstrated that THH extract protects joint inflammation by manipulating the intestinal flora and regulating the TLR4/MyD88/MAPK signaling pathway.Therefore,THH extract may serve as a microbial modulator to recover RA in clincial practice.verRAinclincial practice.展开更多
A wearable UV sensor is designed to realize UV detection and a warning effect on people’s excessive UV exposure.It is noteworthy that the photoelectric system and supporting material of the most conventional sensor a...A wearable UV sensor is designed to realize UV detection and a warning effect on people’s excessive UV exposure.It is noteworthy that the photoelectric system and supporting material of the most conventional sensor are separated.The unstable connection between the two components and the complicated construction method makes the sensor susceptible to external motion interference and prone to failure.Herein,we developed a unique photo response mode of the UV sensor based on a novel photo responsive material.An azobenzene-containing polydimethylsiloxane(Azo-PDMS)film was prepared as the outer layer of the sensor.It integrated the functions of photo response source,support and protection,which realized the direct contact and rapid response to the UV light source.Carbon nanotube(CNT),wrapped in the middle of Azo-PDMS films as an inner layer,transformed the photomechanical response signal into the photoelectric signal.The photo response mode endows the sensor with excellent anti-motion interference capabilities and a true sense of UV-strain synchronous monitoring performance,which is of great significance to the practical application of wearable devices.In addition,based on the excellent properties of Azo-PDMS,the sensor can also protect the human body from UV damage,self-repair after fracture,and realize personalized customization through 3 D printing.It makes a breakthrough in the design and construction of wearable UV sensors and paves a new way to optimize sensor photo response modes.展开更多
基金supported by the Health Commission of Hubei Province of China(No.ZY2021Z005)the Major Science and Technology Project in Yunnan Province(No.202102AE090042)。
文摘Traditionally,Tripterygium hypoglaucum(Levl.)Hutch(THH)are widely used in Chinese folk to treat rheumatoid arthritis(RA).This study aimed to investigate whether the anti-RA effect of THH is related with the gut microbiota.The main components of prepared THH extract were identified by HPLC-MS.C57BL/6 mice with adjuvant-induced arthritis(AIA)were treated with THH extract by gavage for one month.THH extract significantly alleviated swollen ankle,joint cavity exudation,and articular cartilage destruction in AIA mice.The mRNA and protein levels of inflammatory mediators in muscles and plasma indicated that THH extract attenuated inflammatory responses in the joint by blocking TLR4/MyD88/MAPK signaling pathways.THH extract remarkably restored the dysbiosis of the gut microbiota in AIA mice,featuring the increases of Bifidobacterium,Akkermansia,and Lactobacillus and the decreases of Butyricimonas,Parabacteroides,and Anaeroplasma.Furthermore,the altered bacteria were closely correlated with physiological indices and drove metabolic changes of the intestinal microbiota.In addition,antibiotic-induced pseudo germ-free mice were employed to verify the role of the intestinal flora.Strikingly,THH treatment failed to ameliorate the arthritis symptoms and signaling pathways in pseudo germ-free mice,which validates the indispensable role of the intestinal flora.For the first time,we demonstrated that THH extract protects joint inflammation by manipulating the intestinal flora and regulating the TLR4/MyD88/MAPK signaling pathway.Therefore,THH extract may serve as a microbial modulator to recover RA in clincial practice.verRAinclincial practice.
文摘A wearable UV sensor is designed to realize UV detection and a warning effect on people’s excessive UV exposure.It is noteworthy that the photoelectric system and supporting material of the most conventional sensor are separated.The unstable connection between the two components and the complicated construction method makes the sensor susceptible to external motion interference and prone to failure.Herein,we developed a unique photo response mode of the UV sensor based on a novel photo responsive material.An azobenzene-containing polydimethylsiloxane(Azo-PDMS)film was prepared as the outer layer of the sensor.It integrated the functions of photo response source,support and protection,which realized the direct contact and rapid response to the UV light source.Carbon nanotube(CNT),wrapped in the middle of Azo-PDMS films as an inner layer,transformed the photomechanical response signal into the photoelectric signal.The photo response mode endows the sensor with excellent anti-motion interference capabilities and a true sense of UV-strain synchronous monitoring performance,which is of great significance to the practical application of wearable devices.In addition,based on the excellent properties of Azo-PDMS,the sensor can also protect the human body from UV damage,self-repair after fracture,and realize personalized customization through 3 D printing.It makes a breakthrough in the design and construction of wearable UV sensors and paves a new way to optimize sensor photo response modes.
