目的探讨流程简化的液质联用技术(liquid chromatography-tandem mass spectrometry,LC-MS/MS)在大肠埃希菌(Escherichia coli)实现鉴定和鞭毛抗原分型同步联检分析中的应用潜力。方法基于全基因的蛋白质组学细菌鉴定数据库以及包含大...目的探讨流程简化的液质联用技术(liquid chromatography-tandem mass spectrometry,LC-MS/MS)在大肠埃希菌(Escherichia coli)实现鉴定和鞭毛抗原分型同步联检分析中的应用潜力。方法基于全基因的蛋白质组学细菌鉴定数据库以及包含大肠埃希菌所有鞭毛抗原蛋白质的数据库,利用流程简化的LC-MS/MS技术对8株标准菌株以及来源于临床分离出的54株大肠埃希菌同时进行鉴定和鞭毛抗原分型分析;同时使用大肠埃希菌3株标准菌株评估流程简化的LC-MS/MS技术在分型鞭毛抗原时样品稀释倍数和获得的鞭毛抗原肽段数量之间的相关性。结果流程简化的LC-MS/MS技术对62株大肠埃希菌鉴定和鞭毛抗原分型同步联检分析的准确率均为100%;3株大肠埃希菌标准菌株样品系列稀释倍数和获得的鞭毛抗原肽段数量二者之间具有很好的线性关系,相关系数分别为0.99、0.97、0.98。结论流程简化的LC-MS/MS技术能够对大肠埃希菌鉴定和鞭毛抗原分型进行准确的同步联合检测分析,该项技术在大肠埃希菌鉴定和鞭毛抗原分型同步联检分析中优势显著,具有良好的应用前景,未来有望成为临床微生物学和公共卫生领域微生物鉴定、分型及微生态研究的重要工具。展开更多
As an integrated structure,an arch dam is assumed to bear loads in its design consideration.However,multi-defects,such as cracks and the opening of transverse joints,are unavoidable during construction and operation.M...As an integrated structure,an arch dam is assumed to bear loads in its design consideration.However,multi-defects,such as cracks and the opening of transverse joints,are unavoidable during construction and operation.Multi-defects will reduce the structural integrity and stiffness of the dam and affect its working performance and degree of safety.In the current paper,a numerical model of defects and a simulation method of a high arch dam are introduced.The Chencun arch dam is analyzed as a case study.An entire course simulation analysis of the Chencun arch dam from construction to operation is carried out,through which the opening of the transverse and longitudinal joints,formation of cracks,and their influence on deformation and stress of the dam are studied.According to the results of the analysis,appropriate measures should be adopted to prevent the development of cracks,and observation should be strengthened for a more timely discovery of risks.展开更多
文摘目的探讨流程简化的液质联用技术(liquid chromatography-tandem mass spectrometry,LC-MS/MS)在大肠埃希菌(Escherichia coli)实现鉴定和鞭毛抗原分型同步联检分析中的应用潜力。方法基于全基因的蛋白质组学细菌鉴定数据库以及包含大肠埃希菌所有鞭毛抗原蛋白质的数据库,利用流程简化的LC-MS/MS技术对8株标准菌株以及来源于临床分离出的54株大肠埃希菌同时进行鉴定和鞭毛抗原分型分析;同时使用大肠埃希菌3株标准菌株评估流程简化的LC-MS/MS技术在分型鞭毛抗原时样品稀释倍数和获得的鞭毛抗原肽段数量之间的相关性。结果流程简化的LC-MS/MS技术对62株大肠埃希菌鉴定和鞭毛抗原分型同步联检分析的准确率均为100%;3株大肠埃希菌标准菌株样品系列稀释倍数和获得的鞭毛抗原肽段数量二者之间具有很好的线性关系,相关系数分别为0.99、0.97、0.98。结论流程简化的LC-MS/MS技术能够对大肠埃希菌鉴定和鞭毛抗原分型进行准确的同步联合检测分析,该项技术在大肠埃希菌鉴定和鞭毛抗原分型同步联检分析中优势显著,具有良好的应用前景,未来有望成为临床微生物学和公共卫生领域微生物鉴定、分型及微生态研究的重要工具。
基金supported by the National Key Technology R&D Program(Grant No.2008BAB29B05)the National Natural Science Foundation of China(Grant No.50909105)+1 种基金the Public Industry Research Special Funds of MWR(Grant No.200801007)the Research Special Funds of IWHR
文摘As an integrated structure,an arch dam is assumed to bear loads in its design consideration.However,multi-defects,such as cracks and the opening of transverse joints,are unavoidable during construction and operation.Multi-defects will reduce the structural integrity and stiffness of the dam and affect its working performance and degree of safety.In the current paper,a numerical model of defects and a simulation method of a high arch dam are introduced.The Chencun arch dam is analyzed as a case study.An entire course simulation analysis of the Chencun arch dam from construction to operation is carried out,through which the opening of the transverse and longitudinal joints,formation of cracks,and their influence on deformation and stress of the dam are studied.According to the results of the analysis,appropriate measures should be adopted to prevent the development of cracks,and observation should be strengthened for a more timely discovery of risks.
