目的研究变应性鼻炎(AR)、AR伴哮喘综合征(ARAS)患者的尘螨致敏组分蛋白差异及其临床意义。方法回顾性分析42例AR伴或不伴哮喘患者的临床资料,分为AR组和ARAS组。采用ImmunoCAP过敏原检测系统检测体外血清户尘螨和粉尘螨sIgE浓度。同时...目的研究变应性鼻炎(AR)、AR伴哮喘综合征(ARAS)患者的尘螨致敏组分蛋白差异及其临床意义。方法回顾性分析42例AR伴或不伴哮喘患者的临床资料,分为AR组和ARAS组。采用ImmunoCAP过敏原检测系统检测体外血清户尘螨和粉尘螨sIgE浓度。同时采用蛋白芯片法进行户尘螨组分(Der p 1、Der p 2、Der p 7、Der p 10、Der p 21、Der p 23)和粉尘螨组分(Der f 1、Der f 2)检测,分析尘螨过敏原及其组分在AR组和ARAS组中的表达差异。结果31例AR和11例ARAS纳入研究。户尘螨阳性率为100.0%,粉尘螨为97.6%。尘螨组分蛋白致敏率由高到低依次为Der p 1(73.8%)、Der f 1(66.7%)、Der f 2(64.3%)、Der p 2(61.9%)。ARAS组的尘螨组分蛋白Der f 1(100.0%和54.8%,P=0.006)、Der p 2(90.9%和51.6%,P=0.021)、Der f 2(100.0%和51.6%,P=0.004)致敏率显著高于AR组。AR组户尘螨sIgE浓度显著低于ARAS组[(7.65±12.15)kUA/L和(15.20±18.77)kUA/L,P<0.05]。ARAS组Der p 1[(5.39±4.61)kUA/L和(2.03±2.97)kUA/L,P=0.013]、Der p 2[(8.82±13.58)kUA/L和(2.78±5.80)kUA/L,P=0.001]、Der p 23[(1.76±3.88)kUA/L和(0.28±0.65)kUA/L,P<0.001]的sIgE浓度显著高于AR组。相关性分析发现Der p 1、Der p 2、Der f 1、Der f 2具有高度正相关性(P<0.01)。户尘螨组分致敏表现出多重致敏的特点,42例患者中,66.7%出现≥2种的户尘螨组分蛋白阳性,AR组为58.1%,ARAS组为90.9%。ARAS组更倾向于多种组分致敏,≥3种的组分致敏率显著高于AR组(54.6%和29.1%,P<0.05)。结论ARAS较AR患者尘螨浓度更高,Der p 1、Der f 1、Der p 2、Der f 2是主要致敏蛋白组分,致敏率在ARAS患者中更高。Der p 1、Der p 2、Der p 23的浓度在ARAS患者中更高。ARAS患者更倾向于多重组分致敏。展开更多
Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-ba...Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.展开更多
文摘目的研究变应性鼻炎(AR)、AR伴哮喘综合征(ARAS)患者的尘螨致敏组分蛋白差异及其临床意义。方法回顾性分析42例AR伴或不伴哮喘患者的临床资料,分为AR组和ARAS组。采用ImmunoCAP过敏原检测系统检测体外血清户尘螨和粉尘螨sIgE浓度。同时采用蛋白芯片法进行户尘螨组分(Der p 1、Der p 2、Der p 7、Der p 10、Der p 21、Der p 23)和粉尘螨组分(Der f 1、Der f 2)检测,分析尘螨过敏原及其组分在AR组和ARAS组中的表达差异。结果31例AR和11例ARAS纳入研究。户尘螨阳性率为100.0%,粉尘螨为97.6%。尘螨组分蛋白致敏率由高到低依次为Der p 1(73.8%)、Der f 1(66.7%)、Der f 2(64.3%)、Der p 2(61.9%)。ARAS组的尘螨组分蛋白Der f 1(100.0%和54.8%,P=0.006)、Der p 2(90.9%和51.6%,P=0.021)、Der f 2(100.0%和51.6%,P=0.004)致敏率显著高于AR组。AR组户尘螨sIgE浓度显著低于ARAS组[(7.65±12.15)kUA/L和(15.20±18.77)kUA/L,P<0.05]。ARAS组Der p 1[(5.39±4.61)kUA/L和(2.03±2.97)kUA/L,P=0.013]、Der p 2[(8.82±13.58)kUA/L和(2.78±5.80)kUA/L,P=0.001]、Der p 23[(1.76±3.88)kUA/L和(0.28±0.65)kUA/L,P<0.001]的sIgE浓度显著高于AR组。相关性分析发现Der p 1、Der p 2、Der f 1、Der f 2具有高度正相关性(P<0.01)。户尘螨组分致敏表现出多重致敏的特点,42例患者中,66.7%出现≥2种的户尘螨组分蛋白阳性,AR组为58.1%,ARAS组为90.9%。ARAS组更倾向于多种组分致敏,≥3种的组分致敏率显著高于AR组(54.6%和29.1%,P<0.05)。结论ARAS较AR患者尘螨浓度更高,Der p 1、Der f 1、Der p 2、Der f 2是主要致敏蛋白组分,致敏率在ARAS患者中更高。Der p 1、Der p 2、Der p 23的浓度在ARAS患者中更高。ARAS患者更倾向于多重组分致敏。
基金financial supports from Chinese Academy of Sciences"Innovation Fund"from Dalian Institute of Chemical Physics+7 种基金National Hi-Tech Research and Development Program of China(2006AA09Z435)the European Commission(Project:EVK3-CT1999-00005UVTOX)Ph.D.Research Fund from Science and Technology Bureau of Liaoning Province of China(20091019)Laboratory Program from Key Laboratory of Nearshore Marine Environmental Research of Liaoning Higher Education(LS2010024)Science and Technology Program from Ocean and Fisheries Department of Liaoning Province of China(200917)Open Laboratory Program from Key and Open Laboratory of Marine and Estuarine Fisheries Resources and EcologyMinistry of Agriculture of the People's Republic of China(Open-09-13)Public Welfare Projects from State Oceanic Administration(200805030)
文摘Sponges （phylum Porifera） are the phylogenetically oldest metazoa and highly efficient filter feeders. In the marine ecosystem, they are unconditionally exposed to environmental stresses. Understanding the sponge-bacteria interaction is hence of both eco- logical and biological significance. This study investigated the specific interaction between the sponge Hymeniacidon perleve and the non-infectious bacteria, Escherichia coli and infectious bacteria, Vibrio spp. by measuring the 14-3-3 mRNA expression of H. perleve. Three partial cDNAs of 14-3-3 proteins and partial 18S RNA in H. perleve were cloned and sequenced. Using Re- verse-transcription real-time PCR, the 14-3-3 mRNA expression of H. perleve was examined when exposed to three common bacteria in aquatic water--E, coli and two Vibrio spp. for different time and dosages. H. perleve could efficiently remove E. coli from the water column without self-infection; however Vibrio at higher dosages infected H. perleve. When H. perleve was ex- posed to E. coli （1.1×10^7 CFU mL^-1）, V. anguillarum II （1.2×10^6 CFU mL^-1） and V. alginolyticus （3.6×10^5 CFU mL-1） for 6 h, the 14-3-3 mRNA expression in the V. anguillarum II and V. alginolyticus groups was down-regulated by 2.67- and 2.36-fold, respectively. The 14-3-3 mRNA expression in the E. coli group was not significantly different. However, no clear trend was ob- served on the 14-3-3 transcript levels of H. perleve in response to different doses of V. anguillarum II for different time. The re- sults demonstrated that infectious bacteria can be discriminated by 14-3-3 mRNA expression of sponge H. perleve.
