本试验旨在对Lyzl1在雄性小鼠的生殖系统中的表达特性进行初步研究,通过CRISPR/Cas9技术获得了Lyzl1敲除的小鼠模型,从而为进一步确定Lyzl1对雄性小鼠生殖功能的影响奠定基础。利用Real time PCR和Western blotting检测Lyzl1的表达情况...本试验旨在对Lyzl1在雄性小鼠的生殖系统中的表达特性进行初步研究,通过CRISPR/Cas9技术获得了Lyzl1敲除的小鼠模型,从而为进一步确定Lyzl1对雄性小鼠生殖功能的影响奠定基础。利用Real time PCR和Western blotting检测Lyzl1的表达情况。免疫组化检测Lyzl1基因在睾丸和附睾中的定位情况。免疫荧光检测Lyzl1在精子上的定位情况,为预测该基因功能奠定基础。利用CRISPR/Cas9系统,体外转录cas9和sg RNA,把cas9 mRNA和sg RNA按照一定的比例进行受精卵注射和胚胎移植,获得F0代Lyzl1突变小鼠,经过连续交配至F2代获得该基因敲除的纯合小鼠,以期进一步研究其与小鼠生殖的关系。为研究该基因功能提供依据。展开更多
φ200 mm silicon single crystals were grown in the φ450 mm hot zone of a Czochralski （CZ） furnace. By modifying the pattern and the velocity of the argon flow, the silicon single crystals with different oxygen conc...φ200 mm silicon single crystals were grown in the φ450 mm hot zone of a Czochralski （CZ） furnace. By modifying the pattern and the velocity of the argon flow, the silicon single crystals with different oxygen concentrations were obtained. Through numerical simulation, the velocity of the argon gas flow was plotted for the first time. The experiment resuits were analyzed and the optimum condition of the argon flow with the lowest oxygen concentration was obtained.展开更多
基金This project is financially supported by the National Natural Science Foundation of China (No. 60576002).
文摘φ200 mm silicon single crystals were grown in the φ450 mm hot zone of a Czochralski （CZ） furnace. By modifying the pattern and the velocity of the argon flow, the silicon single crystals with different oxygen concentrations were obtained. Through numerical simulation, the velocity of the argon gas flow was plotted for the first time. The experiment resuits were analyzed and the optimum condition of the argon flow with the lowest oxygen concentration was obtained.
