目的对比磁共振酰胺质子转移(Amide Proton Transfer,APT)成像、扩散加权成像(Diffusion Weighted Imaging,DWI)和动态增强(Dynamic Contrast-Enhanced,DCE)成像以及3者联合应用在鉴别乳腺良恶性病变中的诊断价值。方法纳入55例乳腺疾...目的对比磁共振酰胺质子转移(Amide Proton Transfer,APT)成像、扩散加权成像(Diffusion Weighted Imaging,DWI)和动态增强(Dynamic Contrast-Enhanced,DCE)成像以及3者联合应用在鉴别乳腺良恶性病变中的诊断价值。方法纳入55例乳腺疾病患者,均进行APT、DWI以及DCE扫描,测量非对称磁化转移(Magnetization Transfer Asymmetry,MTRasym)率和表观扩散系数(Apparent Diffusion Coefficient,ADC)值,并记录乳腺影像报告和数据系统的分类。分析乳腺良恶性病变各参数之间的差异,使用Logistic回归建立联合诊断模型,采用受试者工作特征曲线(Receive Operating Characteristic,ROC)计算各诊断模型在乳腺良恶性病变中的诊断价值,并比较恶性病变中不同病理因素组间的差异以及各病理因素与APT之间的相关性。结果恶性组中MTRasym和ADC值均小于良性组,差异具有统计学意义(均P<0.05),DWI、APT、DCE以及APT+DCE、APT+DWI、DCE+DWI、DWI+APT+DCE联合模型鉴别乳腺良恶性病变的曲线下面积(Area Under Curve,AUC)分别为0.804、0.723、0.741以及0.782、0.895、0.897、0.970;DWI+APT+DCE联合诊断模型的AUC显著大于DWI、APT、DCE以及任意两者联合诊断模型(均P<0.05)。Ki-67高表达组的MTRasym值较低表达组高[(3.934%±0.883%)v s.(3.192%±0.949%)],乳腺癌浸润程度组织学分级Ⅲ级组的M T R a s y m值较Ⅱ级组高[(4.225%±0.932%)vs.(3.451%±0.873%)],差异均有统计学意义(均P<0.05),并且APT与两者均呈中度正相关(r=0.627、0.537,均P<0.05)。结论APT成像技术在乳腺良恶性病变的鉴别中具有较好的诊断价值,与常规DWI、DCE成像无显著差异,并且三者联合应用的诊断价值更高。展开更多
Genome dynamics of pathogenic organisms are driven by plant host and pathogenic organism co-evolution, in which patho- gen genomes areused to overcome stresses imposed by hosts with various genetic backgrounds through...Genome dynamics of pathogenic organisms are driven by plant host and pathogenic organism co-evolution, in which patho- gen genomes areused to overcome stresses imposed by hosts with various genetic backgrounds through generation of a range of field isolates. This model also applies to the rice host and its fungal pathogen Magnaporthe oryzae. To better understand genetic variation of M. oryzae in nature, the field isolate V86010 from the Philippines was sequenced and ana- lyzed. Genome annotation found that the assembled V86010 genome was composed of 1 931 scaffolds with a combined length of 38.9 Mb. The average GC ratio is 51.3% and repetitive elements constitute 5.1% of the genome. A total of 11 857 genes including 616 effector protein genes were predicted using a combined analysis pipeline. All predicted genes and effector protein genes of isolate V86010 distribute on the eight chromosomes when aligned with the assembled genome of isolate 70-15. Effector protein genes are located disproportionately at several chromosomal ends. The Pot2 elements are abundant in V86010. Seven V86010-specific effector proteins were found to suppress programmed cell death induced by BAX in tobacco leaves using an Agrobacterium-mediated transient assay. Our results may provide useful information for further study of the molecular and genomic dynamics in the evolution of M. oryzae and rice host interactions, and for characterizing novel effectors and AVR genes in the rice blast pathogen.展开更多
文摘目的对比磁共振酰胺质子转移(Amide Proton Transfer,APT)成像、扩散加权成像(Diffusion Weighted Imaging,DWI)和动态增强(Dynamic Contrast-Enhanced,DCE)成像以及3者联合应用在鉴别乳腺良恶性病变中的诊断价值。方法纳入55例乳腺疾病患者,均进行APT、DWI以及DCE扫描,测量非对称磁化转移(Magnetization Transfer Asymmetry,MTRasym)率和表观扩散系数(Apparent Diffusion Coefficient,ADC)值,并记录乳腺影像报告和数据系统的分类。分析乳腺良恶性病变各参数之间的差异,使用Logistic回归建立联合诊断模型,采用受试者工作特征曲线(Receive Operating Characteristic,ROC)计算各诊断模型在乳腺良恶性病变中的诊断价值,并比较恶性病变中不同病理因素组间的差异以及各病理因素与APT之间的相关性。结果恶性组中MTRasym和ADC值均小于良性组,差异具有统计学意义(均P<0.05),DWI、APT、DCE以及APT+DCE、APT+DWI、DCE+DWI、DWI+APT+DCE联合模型鉴别乳腺良恶性病变的曲线下面积(Area Under Curve,AUC)分别为0.804、0.723、0.741以及0.782、0.895、0.897、0.970;DWI+APT+DCE联合诊断模型的AUC显著大于DWI、APT、DCE以及任意两者联合诊断模型(均P<0.05)。Ki-67高表达组的MTRasym值较低表达组高[(3.934%±0.883%)v s.(3.192%±0.949%)],乳腺癌浸润程度组织学分级Ⅲ级组的M T R a s y m值较Ⅱ级组高[(4.225%±0.932%)vs.(3.451%±0.873%)],差异均有统计学意义(均P<0.05),并且APT与两者均呈中度正相关(r=0.627、0.537,均P<0.05)。结论APT成像技术在乳腺良恶性病变的鉴别中具有较好的诊断价值,与常规DWI、DCE成像无显著差异,并且三者联合应用的诊断价值更高。
基金supported by the grants from the National Natural Science Foundation of China (31528017 and 31461143019)
文摘Genome dynamics of pathogenic organisms are driven by plant host and pathogenic organism co-evolution, in which patho- gen genomes areused to overcome stresses imposed by hosts with various genetic backgrounds through generation of a range of field isolates. This model also applies to the rice host and its fungal pathogen Magnaporthe oryzae. To better understand genetic variation of M. oryzae in nature, the field isolate V86010 from the Philippines was sequenced and ana- lyzed. Genome annotation found that the assembled V86010 genome was composed of 1 931 scaffolds with a combined length of 38.9 Mb. The average GC ratio is 51.3% and repetitive elements constitute 5.1% of the genome. A total of 11 857 genes including 616 effector protein genes were predicted using a combined analysis pipeline. All predicted genes and effector protein genes of isolate V86010 distribute on the eight chromosomes when aligned with the assembled genome of isolate 70-15. Effector protein genes are located disproportionately at several chromosomal ends. The Pot2 elements are abundant in V86010. Seven V86010-specific effector proteins were found to suppress programmed cell death induced by BAX in tobacco leaves using an Agrobacterium-mediated transient assay. Our results may provide useful information for further study of the molecular and genomic dynamics in the evolution of M. oryzae and rice host interactions, and for characterizing novel effectors and AVR genes in the rice blast pathogen.
