The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity ...The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.展开更多
PtAUX1,an ortholog of Arabidopsis AtAUX1(encoding auxin influx carrier protein),has been cloned from Populus tomentosa.PtAUX1 protein is similar to Arabidopsis AtAUX1 in structure,but PtAUX1 contains an additional cAM...PtAUX1,an ortholog of Arabidopsis AtAUX1(encoding auxin influx carrier protein),has been cloned from Populus tomentosa.PtAUX1 protein is similar to Arabidopsis AtAUX1 in structure,but PtAUX1 contains an additional cAMP/cGMP-dependent protein kinase phosphorylation site.Its overexpression lines driven by 35S promoter exhibits a novel phenotype related flower development which has not been revealed in AtAUX1 mutants.Ovary-like structures each with 1～2 styles,but without a cup-like disc and a bract,can initial from regenerated transgenic bud in cluster,resembling an inflorescence.The histological dissections illuminate that the ovarylike structures have womb and transmitting tissue inner its styles,but no ovules.PtAUX1 expression pattern are ubiquitous and various in different organs,which is coincident with its candidate auxin influx,functioning in distributing auxin thorough a plant body.These results suggest that PtAUX1 or polar auxin transport has a substantial function in floral organ initiation.展开更多
基金supported by the National Technologies R&D Program of China (2006BAD01A02)the Excellent Medium-Youth Scientist Scientific Research Reward Fundation of Shandong Province, China (BS2009NY036)the Youth Science and Technology Creative Fundation Item of Shandong Agricultural University, China
文摘The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.
基金ShanDong Provincial Education Department Supported Item(no.J07YF08)
文摘PtAUX1,an ortholog of Arabidopsis AtAUX1(encoding auxin influx carrier protein),has been cloned from Populus tomentosa.PtAUX1 protein is similar to Arabidopsis AtAUX1 in structure,but PtAUX1 contains an additional cAMP/cGMP-dependent protein kinase phosphorylation site.Its overexpression lines driven by 35S promoter exhibits a novel phenotype related flower development which has not been revealed in AtAUX1 mutants.Ovary-like structures each with 1～2 styles,but without a cup-like disc and a bract,can initial from regenerated transgenic bud in cluster,resembling an inflorescence.The histological dissections illuminate that the ovarylike structures have womb and transmitting tissue inner its styles,but no ovules.PtAUX1 expression pattern are ubiquitous and various in different organs,which is coincident with its candidate auxin influx,functioning in distributing auxin thorough a plant body.These results suggest that PtAUX1 or polar auxin transport has a substantial function in floral organ initiation.
