Mo_(2)CT_(x)MXene is a novel two-dimensional material,which is generally made by the etching of inorganic acid solutions,such as hydrofluoric acid(HF)or hydrochloric acid(HCl).Those solutions are always corrosive and ...Mo_(2)CT_(x)MXene is a novel two-dimensional material,which is generally made by the etching of inorganic acid solutions,such as hydrofluoric acid(HF)or hydrochloric acid(HCl).Those solutions are always corrosive and hazardous.In this paper,a mild organic acid,acetic acid(CH_(3)COOH),was selected to synthesize Mo_(2)CT_(x)MXene.30 mL acetic acid(HAc)with the concentration of 13 mol/L was mixed with 2 g acetate(CH_(3)COONa or CH_(3)COOK)and 10 mL water to make etching solution(NaAc+HAc or KAc+HAc).In the solution,the concentration of CH_(3)COO^(-)was 10 mol/L,the concentration of Na^(+)/K^(+)is 0.6/0.5 mol/L.The pH value is 2.8.Mo_(2)CT_(x)was obtained by hydrothermal etching at 240℃for 1 d.Compared with the general method of HF etching,the etchant is milder and the etching process is safer.On the surface of Mo_(2)CT_(x)nanosheet made by this method,acetate group(CH_(3)COO^(-))was adsorbed as termination,which is larger than the F/O/OH termination of that made by general HF etching.The lattice parameter c(LP c)of Mo_(2)CT_(x)etched with NaAc+HAc/KAc+HAc is 21.09Å/20.89Å.Moreover,the specific surface areas of the samples etched by NaAc+HAc and KAc+HAc were 18.1 m^(2)/g and 14.1 m^(2)/g,respectively,which were much larger than those etched by conventional methods.As the anode of lithium-ion battery,the specific capacity under current density of 100 mA/g at 100 th cycle was 108 mA·h/g,which is higher than the capacity of samples made by general HF etching.This work reports a novel method to make Mo_(2)CT_(x)MXene by the solution of mild acetic acid.The samples made by this method had very high specific surface area and relatively high lithium-storage performance.展开更多
目的建立检测破伤风抗毒素(TAT)效价的间接ELISA法,并进行初步应用。方法用方阵滴定法确定包被抗原和待检血清的最适工作浓度,并对各种反应条件进行优化,建立间接ELISA法。用建立的间接ELISA法检测3批TAT效价,并与小鼠中和试验法检测结...目的建立检测破伤风抗毒素(TAT)效价的间接ELISA法,并进行初步应用。方法用方阵滴定法确定包被抗原和待检血清的最适工作浓度,并对各种反应条件进行优化,建立间接ELISA法。用建立的间接ELISA法检测3批TAT效价,并与小鼠中和试验法检测结果进行比较。结果建立的间接ELISA法的最适反应条件:抗原最适包被质量浓度为1.2μg/m L,血清最佳稀释浓度为400 m IU/m L;抗原与待检血清作用时间为45 min,加入酶标二抗后作用时间为60 min;抗原最适包被缓冲液为0.05 mol/L p H 9.6碳酸盐(CBS)缓冲液,4℃包被12 h;封闭液为30%BSA(5%蔗糖和5%乳糖);酶标二抗最适工作浓度为1∶8 000。3批TAT间接ELISA法效价检测结果与小鼠中和试验法效价检测结果相比,差异无统计学意义(P均>0.05)。结论建立的间接ELISA法具有较高灵敏度,可用于TAT效价的检测。展开更多
基金Projects(52372284,52275187,62004143)supported by the National Natural Science Foundation of ChinaProject(2022BAA084)supported by the Key R&D Program of Hubei Province,ChinaProject(232300421135)supported by the Natural Science Foundation of Henan Province,China。
文摘Mo_(2)CT_(x)MXene is a novel two-dimensional material,which is generally made by the etching of inorganic acid solutions,such as hydrofluoric acid(HF)or hydrochloric acid(HCl).Those solutions are always corrosive and hazardous.In this paper,a mild organic acid,acetic acid(CH_(3)COOH),was selected to synthesize Mo_(2)CT_(x)MXene.30 mL acetic acid(HAc)with the concentration of 13 mol/L was mixed with 2 g acetate(CH_(3)COONa or CH_(3)COOK)and 10 mL water to make etching solution(NaAc+HAc or KAc+HAc).In the solution,the concentration of CH_(3)COO^(-)was 10 mol/L,the concentration of Na^(+)/K^(+)is 0.6/0.5 mol/L.The pH value is 2.8.Mo_(2)CT_(x)was obtained by hydrothermal etching at 240℃for 1 d.Compared with the general method of HF etching,the etchant is milder and the etching process is safer.On the surface of Mo_(2)CT_(x)nanosheet made by this method,acetate group(CH_(3)COO^(-))was adsorbed as termination,which is larger than the F/O/OH termination of that made by general HF etching.The lattice parameter c(LP c)of Mo_(2)CT_(x)etched with NaAc+HAc/KAc+HAc is 21.09Å/20.89Å.Moreover,the specific surface areas of the samples etched by NaAc+HAc and KAc+HAc were 18.1 m^(2)/g and 14.1 m^(2)/g,respectively,which were much larger than those etched by conventional methods.As the anode of lithium-ion battery,the specific capacity under current density of 100 mA/g at 100 th cycle was 108 mA·h/g,which is higher than the capacity of samples made by general HF etching.This work reports a novel method to make Mo_(2)CT_(x)MXene by the solution of mild acetic acid.The samples made by this method had very high specific surface area and relatively high lithium-storage performance.
文摘目的建立检测破伤风抗毒素(TAT)效价的间接ELISA法,并进行初步应用。方法用方阵滴定法确定包被抗原和待检血清的最适工作浓度,并对各种反应条件进行优化,建立间接ELISA法。用建立的间接ELISA法检测3批TAT效价,并与小鼠中和试验法检测结果进行比较。结果建立的间接ELISA法的最适反应条件:抗原最适包被质量浓度为1.2μg/m L,血清最佳稀释浓度为400 m IU/m L;抗原与待检血清作用时间为45 min,加入酶标二抗后作用时间为60 min;抗原最适包被缓冲液为0.05 mol/L p H 9.6碳酸盐(CBS)缓冲液,4℃包被12 h;封闭液为30%BSA(5%蔗糖和5%乳糖);酶标二抗最适工作浓度为1∶8 000。3批TAT间接ELISA法效价检测结果与小鼠中和试验法效价检测结果相比,差异无统计学意义(P均>0.05)。结论建立的间接ELISA法具有较高灵敏度,可用于TAT效价的检测。
