目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微...目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。展开更多
Global climate change is intensifying the impact of slope hazards,particularly rainfall-induced landslide hazards(RILH),on mountain road networks(MRNs).However,effective quantitative models for dynamically assessing M...Global climate change is intensifying the impact of slope hazards,particularly rainfall-induced landslide hazards(RILH),on mountain road networks(MRNs).However,effective quantitative models for dynamically assessing MRNs vulnerability under RILH disturbances are still lacking.To bridge this gap,this study develops a Cascading Failure Model for Rainfall-Induced Landslide Hazard(CFM-RILH).Validation via a case study of the GarzêTibetan Autonomous Prefecture Road Network(GTPRNs)reveals key characteristics of MRNs system vulnerability under RILH disturbances:(1)Under the disturbance effects of RILH,the vulnerability of the MRNs system follows a nonlinear phase transition law that intensifies with increasing disturbance intensity,exhibiting a distinct critical threshold.When the disturbance intensity exceeds this threshold,the system undergoes a global cascading failure phenomenon analogous to an“avalanche.”(2)Under RILH disturbances,the robustness of the MRNs system possesses a distinct safety boundary.Exceeding this boundary not only fails to improve hazard resistance but instead substantially elevates the risk of large-scale cascading failure.(3)Increasing network redundancy may be considered one of the primary engineering measures for enhancing MRNs resilience against such disturbances.Based on these findings,we propose a“Two-Stage Emergency Response and Hierarchical Fortification”strategy specifically to improve the resilience of GTPRNs impacted by RILH.The CFM-RILH model provides an effective tool for assessing road network vulnerability under such hazards.Furthermore,its modeling framework can also inform vulnerability assessment and resilience strategy development for road networks affected by other types of slope hazards.展开更多
文摘目的:通过对C3H10T1/2细胞分化为成熟棕色脂肪细胞的培养、诱导分化及鉴定,深入探讨棕色脂肪细胞的生物学特性,为人类棕色脂肪细胞的相关研究提供实验参考与理论依据。方法:C3H10T1/2细胞经细胞接种、培养及诱导分化处理,利用光学显微镜观察细胞形态变化,并通过油红O染色、免疫荧光法、线粒体探针法以及线粒体电镜技术对分化细胞进行鉴定分析。结果:未分化的C3H10T1/2细胞形态多样,具有突触伸展特征;分化后的细胞逐渐变为圆形或椭圆形,形成环形脂滴;未分化组细胞形态无明显变化。油红O染色显示,未分化组细胞基本无染色,而分化组中约90%的细胞红染,脂滴分布于细胞核周围,吸光度值显著升高(P<0.05)。分化组解偶联蛋白1(uncoupling protein 1,UCP1)、过氧化物酶体增殖激活受体γ(peroxisome proliferator-activated receptorγ,PPARγ)辅激活因子1α(PPARγco-activator-1α,PGC-1α)、PPARγ和转录因子PRDM16的相对荧光强度和蛋白表达量显著高于未分化组(P<0.05),且线粒体活性增强。结论:本研究成功诱导C3H10T1/2细胞分化为成熟棕色脂肪细胞,结合细胞形态观察、关键蛋白表达检测及线粒体功能分析进行综合评估,证实了细胞的有效分化及成熟棕色脂肪细胞的功能特性。
基金financially supported by the National Key R&D Program of China(2024YFE0111900)The National Natural Science Foundation of China(U2468214,52378370,52278372)+1 种基金The National Ten Thousand Talent Program for Young Top-notch Talents(2022QB04978)The Science and Technology Program of Hebei Province(2023HBQZYCSB004)。
文摘Global climate change is intensifying the impact of slope hazards,particularly rainfall-induced landslide hazards(RILH),on mountain road networks(MRNs).However,effective quantitative models for dynamically assessing MRNs vulnerability under RILH disturbances are still lacking.To bridge this gap,this study develops a Cascading Failure Model for Rainfall-Induced Landslide Hazard(CFM-RILH).Validation via a case study of the GarzêTibetan Autonomous Prefecture Road Network(GTPRNs)reveals key characteristics of MRNs system vulnerability under RILH disturbances:(1)Under the disturbance effects of RILH,the vulnerability of the MRNs system follows a nonlinear phase transition law that intensifies with increasing disturbance intensity,exhibiting a distinct critical threshold.When the disturbance intensity exceeds this threshold,the system undergoes a global cascading failure phenomenon analogous to an“avalanche.”(2)Under RILH disturbances,the robustness of the MRNs system possesses a distinct safety boundary.Exceeding this boundary not only fails to improve hazard resistance but instead substantially elevates the risk of large-scale cascading failure.(3)Increasing network redundancy may be considered one of the primary engineering measures for enhancing MRNs resilience against such disturbances.Based on these findings,we propose a“Two-Stage Emergency Response and Hierarchical Fortification”strategy specifically to improve the resilience of GTPRNs impacted by RILH.The CFM-RILH model provides an effective tool for assessing road network vulnerability under such hazards.Furthermore,its modeling framework can also inform vulnerability assessment and resilience strategy development for road networks affected by other types of slope hazards.
