Intergenerational inheritance of stress memory plays a crucial role in plant adaptation to environmental changes,particularly in the context of spaceflight,where plants may serve as a food source for humans on long-du...Intergenerational inheritance of stress memory plays a crucial role in plant adaptation to environmental changes,particularly in the context of spaceflight,where plants may serve as a food source for humans on long-duration missions.However,the intergenerational genetic effects of spaceflight-induced stress memory in plants remain unclear.In this study,we assessed the cross-generational genetic effects of spaceflight stress memory using the rice mutant B10,identified during the SJ-10 return satellite mission.Our results showed that the oxidative stress effects induced by spaceflight persisted until the M5 generation in rice.We found that the rice genome remained unstable post-spaceflight,leading to alterations in genome methylation levels.Additionally,we observed significant changes in the methylation levels of transposons,suggesting their involvement in the intergenerational inheritance of spaceflight-induced stress memory.Furthermore,we identified thousands of differentially expressed genes(DEGs)and differentially alternatively spliced(DAS)genes induced by spaceflight stress memory across multiple rice generations.Notably,differentially methylated cytosines were more abundant in non-expressed genes than in DEGs or DAS genes.A substantial number of DEGs and DASs related to oxidative stress were identified,primarily involved in the generation and scavenging of reactive oxygen species.This study also presented report on the response of alternative splicing events in rice to spaceflight stress.Moreover,our findings revealed that genome methylation was associated with gene expression but not with DAS.In conclusion,our study provides comprehensive insights into the intergenerational inheritance of spaceflight-induced stress in rice and may contribute to uncovering novel mechanisms of oxidative stress-induced genomic instability and epigenetic regulation in plant stress inheritance.展开更多
As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the o...As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth-and development-related physiological processes and metabolic pathways of rice different stages, the seeds of rice variety DN423 were sent into orbit for 12.5 d aboard the SJ-10 Returning Satellite, and then the seedlings of both treated and control rice were compared at the three-leaf stage(TLS) and tillering stage(TS). In addition to comparing plant growth and reactive oxygen species(ROS) levels, seedling proteomes were also compared using isobaric tags for relative and absolute quantitation(i TRAQ). Space flight increased TLS plant height by 20%, reduced and increased ROS levels of the TLS and TS seedlings, respectively, and affected the expression of 36 and 323 proteins in TLS and TS leaves, respectively. Furthermore, the functions of the differentially abundant proteins were mainly associated with metabolism, energy, and protein synthesis and degradation. These results suggested that the exposure of seeds to outer space conditions affects the subsequent abundance of key signaling proteins, gene expression, and the processes of protein synthesis and degradation, thereby affecting metabolic processes and promoting adaptation to the abiotic stress of outer space. As such, the present study sheds light on the effects of space flight on plants and contributes to a more comprehensive understanding of extraterrestrial biology.展开更多
基金supported by the Natural Science Foundation of Chongqing,China(Grant No.CSTB2024NSCQ-MSX0462)the Postdoctoral Fellowship Program of CPSF,China(Grant No.GZC20242211)+1 种基金the Heilongjiang Touyan Team,China(Grant No.HITTY-20190034)the Project of the Aerospace Science and Technology Collaborative Innovation Center,China(Grant No.BSAUEA5740600223).
文摘Intergenerational inheritance of stress memory plays a crucial role in plant adaptation to environmental changes,particularly in the context of spaceflight,where plants may serve as a food source for humans on long-duration missions.However,the intergenerational genetic effects of spaceflight-induced stress memory in plants remain unclear.In this study,we assessed the cross-generational genetic effects of spaceflight stress memory using the rice mutant B10,identified during the SJ-10 return satellite mission.Our results showed that the oxidative stress effects induced by spaceflight persisted until the M5 generation in rice.We found that the rice genome remained unstable post-spaceflight,leading to alterations in genome methylation levels.Additionally,we observed significant changes in the methylation levels of transposons,suggesting their involvement in the intergenerational inheritance of spaceflight-induced stress memory.Furthermore,we identified thousands of differentially expressed genes(DEGs)and differentially alternatively spliced(DAS)genes induced by spaceflight stress memory across multiple rice generations.Notably,differentially methylated cytosines were more abundant in non-expressed genes than in DEGs or DAS genes.A substantial number of DEGs and DASs related to oxidative stress were identified,primarily involved in the generation and scavenging of reactive oxygen species.This study also presented report on the response of alternative splicing events in rice to spaceflight stress.Moreover,our findings revealed that genome methylation was associated with gene expression but not with DAS.In conclusion,our study provides comprehensive insights into the intergenerational inheritance of spaceflight-induced stress in rice and may contribute to uncovering novel mechanisms of oxidative stress-induced genomic instability and epigenetic regulation in plant stress inheritance.
基金supported by the National Key Research and Development Program of China(Grant No.2017YFC160900)Planning Project for Space Application(Grant No.01-1-08)。
文摘As a unique form of abiotic stress, the environmental conditions of outer space are expected to induce changes in plant genomes, proteomes and metabolic pathways. However, the effect of outer space conditions on the overall physiology of plants at the protein level has yet to be reported. To investigate the effects of outer space conditions on the growth-and development-related physiological processes and metabolic pathways of rice different stages, the seeds of rice variety DN423 were sent into orbit for 12.5 d aboard the SJ-10 Returning Satellite, and then the seedlings of both treated and control rice were compared at the three-leaf stage(TLS) and tillering stage(TS). In addition to comparing plant growth and reactive oxygen species(ROS) levels, seedling proteomes were also compared using isobaric tags for relative and absolute quantitation(i TRAQ). Space flight increased TLS plant height by 20%, reduced and increased ROS levels of the TLS and TS seedlings, respectively, and affected the expression of 36 and 323 proteins in TLS and TS leaves, respectively. Furthermore, the functions of the differentially abundant proteins were mainly associated with metabolism, energy, and protein synthesis and degradation. These results suggested that the exposure of seeds to outer space conditions affects the subsequent abundance of key signaling proteins, gene expression, and the processes of protein synthesis and degradation, thereby affecting metabolic processes and promoting adaptation to the abiotic stress of outer space. As such, the present study sheds light on the effects of space flight on plants and contributes to a more comprehensive understanding of extraterrestrial biology.
