The aim of the present research is to study the mechanism of cervical nerve compression syndrome of the external intervertebral foramen and its differential diagnosis with cervical spondylosis.Diagnostic treatment wit...The aim of the present research is to study the mechanism of cervical nerve compression syndrome of the external intervertebral foramen and its differential diagnosis with cervical spondylosis.Diagnostic treatment with muscle relaxant,vasodilator,neurotrophic medicine and celecoxib(COX)-2 inhibitor were performed in 20 patients with cervical nerve compression syndrome of the external intervertebral foramen and 20 patients with cervical spondylosis confirmed by operation.Diagnostic local block therapy was performed additionally in cases showing little effect after diagnostic treatment.All the patients were followed up postoperatively for more than one year.Fifteen cases with cervical nerve compression syndrome of the external intervertebral foramen were healed by the diagnostic treatment.The other five cases had a short-term remission and there was no recurrence after diagnostic local block therapy.Diagnostic treatment led to short-term alleviation of the symptom in 20 cases with cervical spondylosis confirmed by operation,the results of which was far from satisfactory and operation was undertaken finally in all the 20 cases.The etiology of cervical nerve compression syndrome of the external intervertebral foramen lies in the compression of the cervical plexus,brachial plexus and cervical dorsal rami by the tendinous decussating fibers of the scalenus anticus,medius,minimus and the posterior muscles of the neck.Diagnostic treatment was propitious to differentiate cervical nerve compression syndrome of the external intervertebral foramen from cervical spondylosis.展开更多
The aim of this paper was to observe and visualize the changes in osteoblasts by electron microscopy during osteogenesis using tissue engineering technique.We also studied the feasibility of improving tissue vasculari...The aim of this paper was to observe and visualize the changes in osteoblasts by electron microscopy during osteogenesis using tissue engineering technique.We also studied the feasibility of improving tissue vascularization of the engineered bone by using small intestine submucosa(SIS)as the scaffold.Bone mesenchymal stem cells(BMSCs)were isolated by gradient centrifugation method.Bone mesenchymal stem cells were seeded in the SIS,and the scaffold-cell constructs were cultured in vitro for 2 weeks.Small intestine submucosa without BMSCs served as control.Both SIS scaffolds were then implanted subcutaneously in the dorsa of athymic mice.The implants were harvested after in vivo incubation for 4,8 and 12 weeks.The changes in osteoblasts and vascularization were observed under a transmission electron microscope and a scanning electron microscope.The BMSCs grew quite well,differentiating on the surface of the SIS and secreting a great deal of extracellular matrices.The scaffold-cell constructs formed a lot of bone and blood vessels in vivo.The scaffold degraded after 12 weeks.No osteoblasts,but vascularization and fibroblasts were observed,in the control.The SIS can be used as a scaffold for constructing tissue-engineered bone as it can improve the formation of bone and vessels in vivo.展开更多
基金This study was supported by AO Prevention and Cure Education Fund of the Ministry of Health P R China(No.20040801)Shanghai Youth Venus Plan(Type A)(No.05QMX1438).
文摘The aim of the present research is to study the mechanism of cervical nerve compression syndrome of the external intervertebral foramen and its differential diagnosis with cervical spondylosis.Diagnostic treatment with muscle relaxant,vasodilator,neurotrophic medicine and celecoxib(COX)-2 inhibitor were performed in 20 patients with cervical nerve compression syndrome of the external intervertebral foramen and 20 patients with cervical spondylosis confirmed by operation.Diagnostic local block therapy was performed additionally in cases showing little effect after diagnostic treatment.All the patients were followed up postoperatively for more than one year.Fifteen cases with cervical nerve compression syndrome of the external intervertebral foramen were healed by the diagnostic treatment.The other five cases had a short-term remission and there was no recurrence after diagnostic local block therapy.Diagnostic treatment led to short-term alleviation of the symptom in 20 cases with cervical spondylosis confirmed by operation,the results of which was far from satisfactory and operation was undertaken finally in all the 20 cases.The etiology of cervical nerve compression syndrome of the external intervertebral foramen lies in the compression of the cervical plexus,brachial plexus and cervical dorsal rami by the tendinous decussating fibers of the scalenus anticus,medius,minimus and the posterior muscles of the neck.Diagnostic treatment was propitious to differentiate cervical nerve compression syndrome of the external intervertebral foramen from cervical spondylosis.
基金This work was supported by the National Natural Science Foundation of China(Grant No.30371444).
文摘The aim of this paper was to observe and visualize the changes in osteoblasts by electron microscopy during osteogenesis using tissue engineering technique.We also studied the feasibility of improving tissue vascularization of the engineered bone by using small intestine submucosa(SIS)as the scaffold.Bone mesenchymal stem cells(BMSCs)were isolated by gradient centrifugation method.Bone mesenchymal stem cells were seeded in the SIS,and the scaffold-cell constructs were cultured in vitro for 2 weeks.Small intestine submucosa without BMSCs served as control.Both SIS scaffolds were then implanted subcutaneously in the dorsa of athymic mice.The implants were harvested after in vivo incubation for 4,8 and 12 weeks.The changes in osteoblasts and vascularization were observed under a transmission electron microscope and a scanning electron microscope.The BMSCs grew quite well,differentiating on the surface of the SIS and secreting a great deal of extracellular matrices.The scaffold-cell constructs formed a lot of bone and blood vessels in vivo.The scaffold degraded after 12 weeks.No osteoblasts,but vascularization and fibroblasts were observed,in the control.The SIS can be used as a scaffold for constructing tissue-engineered bone as it can improve the formation of bone and vessels in vivo.
