Nanofiber carbon aerogels with 3D interconnected microfibrillar networks exhibit fascinating physical properties and present great application potential.However,it is still a challenge to fabricate superelastic nanofi...Nanofiber carbon aerogels with 3D interconnected microfibrillar networks exhibit fascinating physical properties and present great application potential.However,it is still a challenge to fabricate superelastic nanofiber carbon aerogels owing to their extremely dilute brittle interconnections and poor fiber toughness after carbonization.Herein,aramid nanofibers(ANF)/nanocellulose(CNF)dual-fibrous carbon aerogels are prepared,which exhibited supercompressibility and superelasticity due to the"skeleton-binder"synergistic effect of ANF and CNF and the design of in-plane micro-wrinkle honeycomb structure.The"skeleton-binder"synergistic effect improves interfacial interactions of nanofibers and optimizes the stress distribution of carbon aerogel.The highly ordered honeycomb structure with in-plane microwrinkles,formed by the bidirectional freezing and the difference in volume shrinkage during the carbonization between CNFs and ANFs,endows the CNF/ANF carbon aerogel with negative Poisson's ratio and high energy absorption capacity.These strategies significantly improve the overall mechanical properties of ANF/CNF carbon aerogel including the elasticity and fatigue resistance.As a result,the ultralight carbon aerogel(3.46 mg/cm^(3))exhibits excellent supercompression(undergoing an extreme strain of 95%)and elasticity(a stress retention up to 81.38% at 90% strain with 500 cycles and 96.15% at 50%strain with 10,000 cycles).The nanofiber carbon aerogel shows excellent multifunctional properties in flexible piezoresistive sensor and anisotropic thermal insulation materials,including a desirable sensitivity(as high as 48.74 kPa^(-1))and an instant response time(~40 ms),an anisotropy factor of 3.69 and an ultralow radial thermal conductivity(0.012 W m^(-1) K^(-1)).These properties make dual-fibrous carbon aerogels highly attractive in pressure sensors and thermal management applications.展开更多
A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by l...A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane （1：1, v/v） using acetophenone as an internal standard （IS）. Analytes were determined in selective ion monitoring （SIM） mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.展开更多
With the rapid development of 5G technology,it has become fast and easy for people to transmit information on the Internet.Digital images can express information more intuitively,so transmitting information through im...With the rapid development of 5G technology,it has become fast and easy for people to transmit information on the Internet.Digital images can express information more intuitively,so transmitting information through images has excellent applications.This paper uses a new chaotic system called 1D-Sin-Logistic-Map(1D-SLM).1D-SLM has two control parameters,which can provide larger parameter space,and the parameter space in the chaotic state is continuous.Through Lyapunov exponent analysis(LE),bifurcation diagrams analysis,spectral entropy analysis(SE),and 0-1 test,it is verified that 1D-SLM has complex dynamic behavior and is very suitable for cryptography.Compared with other 1D chaotic systems,the 1D-SLM has a larger Lyapunov exponent(LE)and spectral entropy(SE).For color image encryption algorithms,only relying on chaotic mapping is not enough to ensure security.So combined with 1D-SLM,we design a color image encryption algorithm,which is implemented by plane expansion,which reduces the correlation between the three channels of color images.The experimental results show that the proposed cross-plane color image encryption algorithm is safe and resistant to common attack methods.展开更多
Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid drople...Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid droplets(LDs)in their cytoplasm during quiescence.Perilipin 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid homeostasis.However,little is known about the role of PLIN 5 in HSC activation.Methods:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection.At the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in NAFLD.The corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy number.Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC-MS/MS.AMPK,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and qPCR.Results:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK activation.In addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver fibrosis.Conclusion:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.展开更多
Fertilization has been shown to exert a significant influence on soil microorganisms and directly and indirectly influences plant growth and survival in agroecosystems. However, it is unknown whether fertilization aff...Fertilization has been shown to exert a significant influence on soil microorganisms and directly and indirectly influences plant growth and survival in agroecosystems. However, it is unknown whether fertilization affects endophytic microbial communities, which are ubiquitous and intimately associated with plant growth and health. Herein, we investigated endophytic bacterial communities in wheat leaves and roots under different long-term fertilization regimes,including NPK chemical fertilizer and NPK chemical fertilizer combined with wheat straw, pig manure, or cow manure. Endophytic bacterial community composition considerably differed in leaves and roots. Although different fertilization treatments did not affect the endophytic bacterial species richness or phylogenetic diversity in either leaves or roots, the community composition was significantly altered, particularly in roots. The endophytic bacterial co-occurrence network in leaves was more complex and stable than that in roots. Furthermore, many of the keystone species that were identified by their topological positions in the co-occurrence networks of leaves and roots were involved in plant growth and fitness. The total relative abundance of keystone species was the highest in the NPK plus cow manure treatment in both leaves and roots. Overall, our results suggest that different fertilization regimes can strongly affect endophytic bacterial communities, and the combination of NPK fertilizer and cow manure promoted the relative abundance of the key endophytic bacterial microbiota in both leaves and roots, which might be beneficial for plants in agroecosystems.展开更多
Hyperspectral remote sensing has emerged as a transformative technology for sustainable natural resource management by providing unprecedented insight into the biochemical,biophysical,and compositional properties of E...Hyperspectral remote sensing has emerged as a transformative technology for sustainable natural resource management by providing unprecedented insight into the biochemical,biophysical,and compositional properties of Earth’s surface.The high spectral resolution of hyperspectral sensors allows a very specific discrimination of materials,monitoring of environmental stress at a very early stage,and provides quantitative retrieval of ecological and geochemical parameters in a wide range of landscapes.The booming technology in sensor design,machine learning,spectral unmixing,and multi-sensor data fusion has further improved the analysis potential and application of imaging spectroscopy to a large extent.This paper involves a discussion of the oversight of such technological advances and the manner in which they are utilized in the principal fields that include forestry,agriculture,water,mineral exploration,and coastal ecosystems.Case studies allow us to identify the potential practical consequences of both spaceborne and unmanned aerial vehicles(UAV)-based hyperspectral systems and AI-based workflows that can be used to aid in more efficient and accurate environmental review.Even though the issues associated with data volume,atmospheric impacts,lack of uniformity in the calibration process,and socioeconomic limits continue to exist,the new technology in sensor miniaturization,cloud computing,and artificial intelligence indicates a fast-changing environment.All these developments make hyperspectral remote sensing a key instrument in solving global sustainability problems and evidence-based management of natural resources in an evolving world.展开更多
Partitioning of soil organic matter for particulate organic carbon(POC)and mineral-associated organic carbon(MAOC)is essential to understand carbon(C)storage under climate change,given their distinct properties and re...Partitioning of soil organic matter for particulate organic carbon(POC)and mineral-associated organic carbon(MAOC)is essential to understand carbon(C)storage under climate change,given their distinct properties and response to warming.The mechanisms underlying warming-induced changes in C pools in black soils(Mollisols)remain unknown,owing to the stability of C pools and the complexity of their associated microbial communities.This study elucidates POC and MAOC contents and their microbial controls in black soils along a mean annual temperature(MAT)gradient from 0.6 to 7.3℃.The POC content(3.3-17 g kg^(−1))increased with MAT,while MAOC content(33-60 g kg^(−1))decreased,indicating accelerated C turnover with warming.Higher MAT shifted the bacterial communities from K-to r-strategies,aligning with increased POC content.The dominance of r-strategists facilitated rapid utilization and mineralization of organic compounds(e.g.,mainly with low C/N ratio),reducing MAOC and increasing POC through sustained plant residue inputs.This shift towards r-strategists also corresponded with increased abundance of saprotrophic fungi and stronger bacteria-saprotrophic fungi associations.Warming in colder regions may release available organic matter that saprotrophic fungi preferentially utilize over plant residues to minimize energy expenditure,decreasing POC decomposition.Our findings suggest that integrating microbial r-/K-strategies help to elucidate these mechanisms and simplify the interpretation of temperature effects on the dynamics of two main functional pools of soil organic matter.展开更多
Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales....Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales.Here,we surveyed 494 pairwise fungal communities in leaves and soils by Illumina sequencing,which were associated with 55 woody plant species across more than 2,000-km span of mountain forests in eastern China.The relative contributions of plant,climate,soil and space to the variation of fungal communities were assessed,and the plant-fungus network topologies were inferred.Plant phylogeny was the strongest predictor for fungal community composition in leaves,accounting for 19.1%of the variation.In soils,plant phylogeny,climatic factors and soil properties explained 9.2%,9.0%and 8.7%of the variation in soil fungal community,respectively.The plant-fungus networks in leaves exhibited significantly higher specialization,modularity and robustness(resistance to node loss),but less complicated topology(e.g.,significantly lower linkage density and mean number of links)than those in soils.In addition,host/fungus preference combinations and key species,such as hubs and connectors,in bipartite networks differed strikingly between aboveground and belowground samples.The findings provide novel insights into cross-kingdom(plant-fungus)species co-occurrence at large spatial scales.The data further suggest that community shifts of trees due to climate change or human activities will impair aboveground and belowground forest fungal diversity in different ways.展开更多
Soil pH was the key factor influencing the phoDharboring bacterial networks.Identification of a cluster positively linked to ALP activity and plant P uptake.Low soil pH resulted in a severe loss of phoDharboring bacte...Soil pH was the key factor influencing the phoDharboring bacterial networks.Identification of a cluster positively linked to ALP activity and plant P uptake.Low soil pH resulted in a severe loss of phoDharboring bacterial core cluster.Fertilization treatments profoundly influence the bacterial communities associated with soil organic phosphorus(P)mineralization and alkaline phosphatase(ALP)activity.However,the relationships among the phoD-harboring bacterial communities associated with soil organic P mineralization,soil ALP activity,and plant P uptake under long-term fertilization remain unexplored.This study investigated these associations at the wheat rapid growth stage in a 40-year fertilization experiment.NPK fertilization led to a significant decrease in the diversity of phoD-harboring bacteria,which could be partially mitigated by the addition of organic materials.Soil pH emerged as the key factor influencing the structure and diversity of the phoD-harboring bacterial community.Furthermore,fertilizations involving manure additions resulted in more stable and cooperative phoD-harboring bacterial co-occurrence networks,compared to NPK fertilization.A functional phoD-harboring bacterial cluster,comprising genera Nostoc,Bradyrhizobium,and Pseudomonas,was identified,showing a positive association with soil ALP activity and plant P uptake.In summary,our study highlights the significant role of the identified core cluster of phoD-harboring bacteria in maintaining soil ALP activity and promoting plant P uptake,in decades of fertilization.Moreover,this study inferred a list of phoD-harboring bacterial genera from the core cluster,with established links to both plant P uptake and soil organic P mineralization.These findings offer valuable insights for sustainable agricultural practices.展开更多
文摘Nanofiber carbon aerogels with 3D interconnected microfibrillar networks exhibit fascinating physical properties and present great application potential.However,it is still a challenge to fabricate superelastic nanofiber carbon aerogels owing to their extremely dilute brittle interconnections and poor fiber toughness after carbonization.Herein,aramid nanofibers(ANF)/nanocellulose(CNF)dual-fibrous carbon aerogels are prepared,which exhibited supercompressibility and superelasticity due to the"skeleton-binder"synergistic effect of ANF and CNF and the design of in-plane micro-wrinkle honeycomb structure.The"skeleton-binder"synergistic effect improves interfacial interactions of nanofibers and optimizes the stress distribution of carbon aerogel.The highly ordered honeycomb structure with in-plane microwrinkles,formed by the bidirectional freezing and the difference in volume shrinkage during the carbonization between CNFs and ANFs,endows the CNF/ANF carbon aerogel with negative Poisson's ratio and high energy absorption capacity.These strategies significantly improve the overall mechanical properties of ANF/CNF carbon aerogel including the elasticity and fatigue resistance.As a result,the ultralight carbon aerogel(3.46 mg/cm^(3))exhibits excellent supercompression(undergoing an extreme strain of 95%)and elasticity(a stress retention up to 81.38% at 90% strain with 500 cycles and 96.15% at 50%strain with 10,000 cycles).The nanofiber carbon aerogel shows excellent multifunctional properties in flexible piezoresistive sensor and anisotropic thermal insulation materials,including a desirable sensitivity(as high as 48.74 kPa^(-1))and an instant response time(~40 ms),an anisotropy factor of 3.69 and an ultralow radial thermal conductivity(0.012 W m^(-1) K^(-1)).These properties make dual-fibrous carbon aerogels highly attractive in pressure sensors and thermal management applications.
基金supported by the National Natural Science Foundation of China(Nos.81073143 and 81373926)Research Fund for the Doctoral Program of Higher Education of China(No. 20092134110004)
文摘A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane （1：1, v/v） using acetophenone as an internal standard （IS）. Analytes were determined in selective ion monitoring （SIM） mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.
基金This research was supported by the National Natural Science Foundation of China(61802212).
文摘With the rapid development of 5G technology,it has become fast and easy for people to transmit information on the Internet.Digital images can express information more intuitively,so transmitting information through images has excellent applications.This paper uses a new chaotic system called 1D-Sin-Logistic-Map(1D-SLM).1D-SLM has two control parameters,which can provide larger parameter space,and the parameter space in the chaotic state is continuous.Through Lyapunov exponent analysis(LE),bifurcation diagrams analysis,spectral entropy analysis(SE),and 0-1 test,it is verified that 1D-SLM has complex dynamic behavior and is very suitable for cryptography.Compared with other 1D chaotic systems,the 1D-SLM has a larger Lyapunov exponent(LE)and spectral entropy(SE).For color image encryption algorithms,only relying on chaotic mapping is not enough to ensure security.So combined with 1D-SLM,we design a color image encryption algorithm,which is implemented by plane expansion,which reduces the correlation between the three channels of color images.The experimental results show that the proposed cross-plane color image encryption algorithm is safe and resistant to common attack methods.
基金Discipline Key Special ProjectGrant/Award Number:XKZDQY202001+7 种基金Henan Provincial Key R&D and Promotion Special ProjectGrant/Award Number:212102310033Henan Provincial Medical Science and Technology Tackling ProgramGrant/Award Number:LHGJ20220557Key R&D Program of ChinaGrant/Award Number:2020YFC2006100,2020YFC2009000 and 2020YFC2009006National Natural Science Foundation of ChinaGrant/Award Number:31471330 and 81870408。
文摘Background:Nonalcoholic fatty liver disease(NAFLD)is one of the most common chronic liver diseases globally.Hepatic stellate cells(HSCs)are the major effector cells of liver fibrosis.HSCs contain abundant lipid droplets(LDs)in their cytoplasm during quiescence.Perilipin 5(PLIN 5)is a LD surface-associated protein that plays a crucial role in lipid homeostasis.However,little is known about the role of PLIN 5 in HSC activation.Methods:PLIN 5 was overexpressed in HSCs of Sprague–Dawley rats by lentivirus transfection.At the same time,PLIN 5 gene knockout mice were constructed and fed with a high-fat diet(HFD)for 20 weeks to study the role of PLIN 5 in NAFLD.The corresponding reagent kits were used to measure TG,GSH,Caspase 3 activity,ATP level,and mitochondrial DNA copy number.Metabolomic analysis of mice liver tissue metabolism was performed based on UPLC-MS/MS.AMPK,mitochondrial function,cell proliferation,and apoptosis-related genes and proteins were detected by western blotting and qPCR.Results:Overexpression of PLIN 5 in activated HSCs led to a decrease in ATP levels in mitochondria,inhibition of cell proliferation,and a significant increase in cell apoptosis through AMPK activation.In addition,compared with the HFD-fed C57BL/6J mice,PLIN 5 knockout mice fed with HFD showed reduced liver fat deposition,decreased LD abundance and size,and reduced liver fibrosis.Conclusion:These findings highlight the unique regulatory role of PLIN 5 in HSCs and the role of PLIN 5 in the fibrosis process of NAFLD.
基金funded by the National Natural Science Foundation of China (No.31870480)the Strategic Priority Research Program of Chinese Academy of Sciences (No.XDB15010101)the China Biodiversity Observation Networks (Sino BON)。
文摘Fertilization has been shown to exert a significant influence on soil microorganisms and directly and indirectly influences plant growth and survival in agroecosystems. However, it is unknown whether fertilization affects endophytic microbial communities, which are ubiquitous and intimately associated with plant growth and health. Herein, we investigated endophytic bacterial communities in wheat leaves and roots under different long-term fertilization regimes,including NPK chemical fertilizer and NPK chemical fertilizer combined with wheat straw, pig manure, or cow manure. Endophytic bacterial community composition considerably differed in leaves and roots. Although different fertilization treatments did not affect the endophytic bacterial species richness or phylogenetic diversity in either leaves or roots, the community composition was significantly altered, particularly in roots. The endophytic bacterial co-occurrence network in leaves was more complex and stable than that in roots. Furthermore, many of the keystone species that were identified by their topological positions in the co-occurrence networks of leaves and roots were involved in plant growth and fitness. The total relative abundance of keystone species was the highest in the NPK plus cow manure treatment in both leaves and roots. Overall, our results suggest that different fertilization regimes can strongly affect endophytic bacterial communities, and the combination of NPK fertilizer and cow manure promoted the relative abundance of the key endophytic bacterial microbiota in both leaves and roots, which might be beneficial for plants in agroecosystems.
基金supported by the Shandong Province Higher Education Institutions New Technology R&D Platform—Spatiotemporal IoT Cloud Application New Technology R&D Center,Shandong Vocational Education Skill Master Studio—Zhao Yaqian Skill Master Studio,and Shandong University of Engineering and Vocational Technology.
文摘Hyperspectral remote sensing has emerged as a transformative technology for sustainable natural resource management by providing unprecedented insight into the biochemical,biophysical,and compositional properties of Earth’s surface.The high spectral resolution of hyperspectral sensors allows a very specific discrimination of materials,monitoring of environmental stress at a very early stage,and provides quantitative retrieval of ecological and geochemical parameters in a wide range of landscapes.The booming technology in sensor design,machine learning,spectral unmixing,and multi-sensor data fusion has further improved the analysis potential and application of imaging spectroscopy to a large extent.This paper involves a discussion of the oversight of such technological advances and the manner in which they are utilized in the principal fields that include forestry,agriculture,water,mineral exploration,and coastal ecosystems.Case studies allow us to identify the potential practical consequences of both spaceborne and unmanned aerial vehicles(UAV)-based hyperspectral systems and AI-based workflows that can be used to aid in more efficient and accurate environmental review.Even though the issues associated with data volume,atmospheric impacts,lack of uniformity in the calibration process,and socioeconomic limits continue to exist,the new technology in sensor miniaturization,cloud computing,and artificial intelligence indicates a fast-changing environment.All these developments make hyperspectral remote sensing a key instrument in solving global sustainability problems and evidence-based management of natural resources in an evolving world.
基金supported by the National Key Research and Development Program of China[Grant No.2022YFD1500202]Strategic Priority Research Program of the Chinese Academy of Sciences[Grant No.XDA28020202]+1 种基金China Postdoctoral Science Foundation[Grant No.2024M753332]the RUDN University Strategic Academic Leadership Program.
文摘Partitioning of soil organic matter for particulate organic carbon(POC)and mineral-associated organic carbon(MAOC)is essential to understand carbon(C)storage under climate change,given their distinct properties and response to warming.The mechanisms underlying warming-induced changes in C pools in black soils(Mollisols)remain unknown,owing to the stability of C pools and the complexity of their associated microbial communities.This study elucidates POC and MAOC contents and their microbial controls in black soils along a mean annual temperature(MAT)gradient from 0.6 to 7.3℃.The POC content(3.3-17 g kg^(−1))increased with MAT,while MAOC content(33-60 g kg^(−1))decreased,indicating accelerated C turnover with warming.Higher MAT shifted the bacterial communities from K-to r-strategies,aligning with increased POC content.The dominance of r-strategists facilitated rapid utilization and mineralization of organic compounds(e.g.,mainly with low C/N ratio),reducing MAOC and increasing POC through sustained plant residue inputs.This shift towards r-strategists also corresponded with increased abundance of saprotrophic fungi and stronger bacteria-saprotrophic fungi associations.Warming in colder regions may release available organic matter that saprotrophic fungi preferentially utilize over plant residues to minimize energy expenditure,decreasing POC decomposition.Our findings suggest that integrating microbial r-/K-strategies help to elucidate these mechanisms and simplify the interpretation of temperature effects on the dynamics of two main functional pools of soil organic matter.
基金supported by the NSFC-NSF Dimensions of Biodiversity Program(31461123001)the National Natural Science Foundation of China(41907039,42277308)+3 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(XDA28020202)the National Key R&D Program of China(2022YFD1500202)the US National Science Foundation(DEB-1442280)to PSS and DESthe China Biodiversity Observation Network(Sino BON)。
文摘Plant and fungal species interactions drive many essential ecosystem properties and processes;however,how these interactions differ between aboveground and belowground habitats remains unclear at large spatial scales.Here,we surveyed 494 pairwise fungal communities in leaves and soils by Illumina sequencing,which were associated with 55 woody plant species across more than 2,000-km span of mountain forests in eastern China.The relative contributions of plant,climate,soil and space to the variation of fungal communities were assessed,and the plant-fungus network topologies were inferred.Plant phylogeny was the strongest predictor for fungal community composition in leaves,accounting for 19.1%of the variation.In soils,plant phylogeny,climatic factors and soil properties explained 9.2%,9.0%and 8.7%of the variation in soil fungal community,respectively.The plant-fungus networks in leaves exhibited significantly higher specialization,modularity and robustness(resistance to node loss),but less complicated topology(e.g.,significantly lower linkage density and mean number of links)than those in soils.In addition,host/fungus preference combinations and key species,such as hubs and connectors,in bipartite networks differed strikingly between aboveground and belowground samples.The findings provide novel insights into cross-kingdom(plant-fungus)species co-occurrence at large spatial scales.The data further suggest that community shifts of trees due to climate change or human activities will impair aboveground and belowground forest fungal diversity in different ways.
基金supported by the National Natural Science Foundation of China(Grant No.42307402).
文摘Soil pH was the key factor influencing the phoDharboring bacterial networks.Identification of a cluster positively linked to ALP activity and plant P uptake.Low soil pH resulted in a severe loss of phoDharboring bacterial core cluster.Fertilization treatments profoundly influence the bacterial communities associated with soil organic phosphorus(P)mineralization and alkaline phosphatase(ALP)activity.However,the relationships among the phoD-harboring bacterial communities associated with soil organic P mineralization,soil ALP activity,and plant P uptake under long-term fertilization remain unexplored.This study investigated these associations at the wheat rapid growth stage in a 40-year fertilization experiment.NPK fertilization led to a significant decrease in the diversity of phoD-harboring bacteria,which could be partially mitigated by the addition of organic materials.Soil pH emerged as the key factor influencing the structure and diversity of the phoD-harboring bacterial community.Furthermore,fertilizations involving manure additions resulted in more stable and cooperative phoD-harboring bacterial co-occurrence networks,compared to NPK fertilization.A functional phoD-harboring bacterial cluster,comprising genera Nostoc,Bradyrhizobium,and Pseudomonas,was identified,showing a positive association with soil ALP activity and plant P uptake.In summary,our study highlights the significant role of the identified core cluster of phoD-harboring bacteria in maintaining soil ALP activity and promoting plant P uptake,in decades of fertilization.Moreover,this study inferred a list of phoD-harboring bacterial genera from the core cluster,with established links to both plant P uptake and soil organic P mineralization.These findings offer valuable insights for sustainable agricultural practices.
