Golgi membrane protein 1(GOLM1/GP73)is a serum marker of hepatocellular carcinoma(HCC).We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression.In this study,we demonstrate...Golgi membrane protein 1(GOLM1/GP73)is a serum marker of hepatocellular carcinoma(HCC).We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression.In this study,we demonstrated that the mammalian target of rapamycin(mTOR)was a negative regulator of microRNA-145(miR-145)expression.miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene.GOLM1 and miR-145 were inversely correlated in human HCC tissues.GOLM1-enriched exosomes activated the glycogen synthase kinase-3β/matrix metalloproteinases(GSK-3β/MMPs)signaling axis of recipient cells and accelerated cell proliferation and migration.In contrast,miR-145 suppressed tumorigenesis and metastasis.We suggest that mTOR/miR-145/GOLM1 signaling pathway should be targeted for HCC treatment.展开更多
Recently,theγ’-strengthened superalloys are of great interests in high temperature applications due to their excellent high temperature strength which is derived from theγ’strengthening phase.For theseγ’-strengt...Recently,theγ’-strengthened superalloys are of great interests in high temperature applications due to their excellent high temperature strength which is derived from theγ’strengthening phase.For theseγ’-strengthened superalloys,the changes in morphology,size and distribution ofγ’precipitates due to coarsening during thermal exposure have a significant impact on the properties of alloys.This article briefly summarizes the recent advances on the coarsening behavior of gamma prime precipitates in the recently-developedγ’-strengthened superalloys and its effects on the hot deformation behavior of superalloys,drawing specific examples on Allvac^(■)718 Plus TM and Ni3 Al-based intermetallic superalloys.It is found that the particle size plays an important role in morphological evolution ofγ’precipitates.For instance,the morphology ofγ’precipitates evolves from cuboidal to strip-like or other complex structures in Ni3 Al-based intermetallic alloys,while theγ’precipitates in Allvac^(■)718 Plus alloy always present nearspherical morphology due to the relatively small initial particle size.The Lifshitz-Slyozof-Wagner(LSW)theory and its modifications,as well as Trans-Interface Diffusion Controlled(TIDC)theory have been applied to describing the coarsening kinetics ofγ’precipitates.Additionally,the hot deformation behavior ofγ’-strengthened superalloy is found to be greatly influenced by the coarsening ofγ’precipitates.展开更多
Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natu...Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natural orifices specimen extractions(NOSE)were performed in China.The animal experiments included five kidney biopsies,24 nephrectomies and six partial nephrectomies.The clinical surgeries included 12 transvaginal NOSE(TV-NOSE),266 hybrid transvaginal NOTES(TV-NOTES)and 27 pure TV-NOTES.The TV-NOSE procedure was performed in five transumbilical laparoendoscopic single-site(U-LESS)nephrectomies,four suprapubic-assisted laparoendoscopic single-site surgery(SA-LESS)nephroureterectomies,and three laparoscopic radical cystectomies.The hybrid TV-NOTES procedure included 210 nephrectomies,31 adrenalectomies,eight nephroureterectomies,13 partial nephrectomies,and four heminephrectomies.The pure TV-NOTES procedure included five renal cyst decortications and 22 nephrectomies.Results:A total of 29 animal experiments were successfully performed.One partial nephrectomy was converted to standard laparoscopic surgery.Two kidney biopsies and two nephrectomies were unsuccessful.A total of 297 clinical surgeries were successfully performed.Six patients who underwent hybrid TV-NOTES were converted to open surgery.Two patients who underwent pure TV-NOTES were converted to SA-LESS.There were 22 major complications,16 occurred intraoperatively and six postoperatively.The mean visual analog score(VAS)of 48 h after the operation was 2.5 points in TV-NOSE,2.3 points in hybrid TV-NOTES and 1.7 points in pure TV-NOTES.The mean follow-up of 50.6(3.0-87.0)months showed that all patients were in good condition.The umbilicus scars were nearly invisible in TV-NOSE and hybrid TV-NOTES.The vaginal incision healed well.Conclusions:TV-NOSE and TV-NOTES are feasible,safe,and effective with little injury,low pain,fast recovery,and good cosmetic outcomes in properly selected patients.They are worth consideration for urological clinical practice.展开更多
Tiered Mobile Wireless Sensor Network(TMWSN)is a new paradigm introduced by mobile edge computing.Now it has received wide attention because of its high scalability,robustness,deployment flexibility,and it has a wide ...Tiered Mobile Wireless Sensor Network(TMWSN)is a new paradigm introduced by mobile edge computing.Now it has received wide attention because of its high scalability,robustness,deployment flexibility,and it has a wide range of application scenarios.In TMWSNs,the storage nodes are the key nodes of the network and are more easily captured and utilized by attackers.Once the storage nodes are captured by the attackers,the data stored on them will be exposed.Moreover,the query process and results will not be trusted any more.This paper mainly studies the secure KNN query technology in TMWSNs,and we propose a secure KNN query algorithm named the Basic Algorithm For Secure KNN Query(BAFSKQ)first,which can protect privacy and verify the integrity of query results.However,this algorithm has a large communication overhead in most cases.In order to solve this problem,we propose an improved algorithm named the Secure KNN Query Algorithm Based on MR-Tree(SEKQAM).The MR-Trees are used to find the K-nearest locations and help to generate a verification set to process the verification of query results.It can be proved that our algorithms can effectively guarantee the privacy of the data stored on the storage nodes and the integrity of the query results.Our experimental results also show that after introducing the MR-Trees in KNN queries on TMWSNs,the communication overhead has an effective reduction compared to BAFSKQ.展开更多
The Ross-Amundsen sector is experiencing an accelerating warming trend and a more intensive advective influx of marine air streams.As a result,massive surface melting events of the ice shelf are occurring more frequen...The Ross-Amundsen sector is experiencing an accelerating warming trend and a more intensive advective influx of marine air streams.As a result,massive surface melting events of the ice shelf are occurring more frequently,which puts the West Antarctica Ice Sheet at greater risk of degradation.This study shows the connection between surface melting and the prominent intrusion of warm and humid air flows from lower latitudes.By applying the Climate Feedback-Response Analysis Method(CFRAM),the temporal surge of the downward longwave(LW)fluxes over the surface of the Ross Ice Shelf(RIS)and adjacent regions are identified for four historically massive RIS surface melting events.The melting events are decomposed to identify which physical mechanisms are the main contributors.We found that intrusions of warm and humid airflow from lower latitudes are conducive to warm air temperature and water vapor anomalies,as well as cloud development.These changes exert a combined impact on the abnormal enhancement of the downward LW surface radiative fluxes,significantly contributing to surface warming and the resultant massive melting of ice.展开更多
Background:The expression of pyruvate kinase muscle 2(PKM2)is augmented in macrophages of patients with atherosclerotic coronary artery disease.The role of PKM2 in atherosclerosis is to be determined.Methods:Global an...Background:The expression of pyruvate kinase muscle 2(PKM2)is augmented in macrophages of patients with atherosclerotic coronary artery disease.The role of PKM2 in atherosclerosis is to be determined.Methods:Global and myeloid cell-specific PKM2 knock-in mice with ApoE^(-/-)background(ApoE^(-/-),PKM2^(KI/KI)and Lyz2-cre,ApoE^(-/-),and PKM2^(flox/flox))were produced to evaluate the clinical significance of PKM2 in atherosclerosis development.Wild-type and PKM2 knock-in macrophages were isolated to assess the function of PKM2 in macrophage phagocytosis.Atherosclerotic mice were treated with PKM2 inhibitor shikonin(SKN)to evaluate the therapeutic potential of PKM2 suppression in atherosclerosis.Results:Oxidized low-density lipoprotein(oxLDL)upregulated PKM2 in macrophages.PKM2 in return promoted the uptake of oxLDL by macrophages.Overexpressed PKM2 accelerated atherosclerosis in mice.SKN blocked the progress of mouse atherosclerosis.Conclusions:PKM2 accelerates macrophage phagocytosis and atherosclerosis.Targeting PKM2 is a potential therapy for atherosclerosis.展开更多
Driven by the increase in CO_(2)concentration,climate models reach a consensus that the large-scale circulation of the South Asian summer monsoon(SASM) becomes weakened but with different magnitudes.This study investi...Driven by the increase in CO_(2)concentration,climate models reach a consensus that the large-scale circulation of the South Asian summer monsoon(SASM) becomes weakened but with different magnitudes.This study investigates the major uncertainty sources of the SASM response to an abrupt quadrupling of CO_(2)(abrupt-4×CO_(2))in 18 models of phase 6 of the Coupled Model Intercomparison Project.The projected weakening of the SASM indicated by both zonal and meridional monsoon circulation indices is closely linked to decreases in the meridional gradient of upper-tropospheric temperature between Eurasia and the Indian Ocean(EUTT-IUTT).A climate feedback-response analysis method is applied to linearly decompose the uncertainty of changes in EUTT-IUTT into the partial changes due to external forcing and internal processes of the earth-atmosphere column.Results show that the uncertainty of changes in EUTT-IUTT is contributed positively by the dominant atmospheric dynamic process,followed by the cloud shortwave radiative effect,and negatively by the surface latent heat flux and cloud longwave radiative effect.Contributions from CO_(2)forcing and other internal processes including albedo and water vapor feedbacks,oceanic heat storage,and sensible heat flux are found to be minor.展开更多
Based on the traditional Human-Computer Interaction method which is mainly touch input system, the way of capturing the movement of people by using cameras is proposed. This is a convenient technique which can provide...Based on the traditional Human-Computer Interaction method which is mainly touch input system, the way of capturing the movement of people by using cameras is proposed. This is a convenient technique which can provide users more experience. In the article, a new way of detecting moving things is given on the basis of development of the image processing technique. The system architecture decides that the communication should be used between two different applications. After considered, named pipe is selected from many ways of communication to make sure that video is keeping in step with the movement from the analysis of the people moving. According to a large amount of data and principal knowledge, thinking of the need of actual project, a detailed system design and realization is finished. The system consists of three important modules: detecting of the people's movement, information transition between applications and video showing in step with people's movement. The article introduces the idea of each module and technique.展开更多
Since the 1980s,educational experts and scholars from western countries have carried out multi-level and multi-angle research on“reflection,”“reflective teaching,”“reflective teacher,”and other subject matters,a...Since the 1980s,educational experts and scholars from western countries have carried out multi-level and multi-angle research on“reflection,”“reflective teaching,”“reflective teacher,”and other subject matters,and fruitful results have been achieved[1].Since the late 1990s,the research on reflective teachers in China has gradually deepened.Not only a considerable number of papers have been published,but also numerous monographs.This paper defines the term“reflective teacher,”explores and studies the nurturing of reflective teachers,clarifies the relationship between reflection and classroom,and highlights the characteristics that reflective teachers should have.展开更多
The development of multifunctional intelligent textiles has become an important innovation direction in the field of textile engineering, under the dual demands of intelligent health monitoring and environmental prote...The development of multifunctional intelligent textiles has become an important innovation direction in the field of textile engineering, under the dual demands of intelligent health monitoring and environmental protection. Although singlefunctional textiles with antibacterial, photochromic, and strain-sensing properties have been developed, they are unable to meet the demand for multifunctional textiles. In this respect, this study developed a poly(lactic acid) and poly(3-hydroxybutyrate)/thermoplastic polyurethane/carbon black nanoparticle composite nanofiber yarn(PPTCY) with a hollow-core-sheath structure using a simple conjugate electrospinning technology. PPTCY possessed excellent mechanical strength and could be effectively woven. More importantly, it not only enabled real-time visual monitoring of ultraviolet(UV) intensity in the environment but also possessed excellent antibacterial properties and strain-sensing performance. Its ΔE value was up to 58.24, and its antibacterial rates against Escherichia coli and Staphylococcus aureus were both 99.99%. This fabric had excellent strainsensing performance, high linearity, and durability under both pressure and stretching deformations. This research provides favorable technical support for the application of intelligent textiles in the field of UV protection and traffic safety.展开更多
Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in m...Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation transcriptionally.However,the molecular mechanisms underlying the orchestration are not fully understood.Here,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription factor.Importantly,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury model.Moreover,miR-130b expression is declined in the muscle of muscular dystrophy patients.Thus,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy progression.Together,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.展开更多
Carbon nanofibers(CNFs)with excellent conductivity and stability have become a promising material to design the strain sensing network.To date,however,the effect of the stacked structure of CNF membrane on the sensing...Carbon nanofibers(CNFs)with excellent conductivity and stability have become a promising material to design the strain sensing network.To date,however,the effect of the stacked structure of CNF membrane on the sensing performance has rarely been stu-died.In this work,we reported a high-performance sensor based on the cross-stacked aligned CNF membrane.The effects of crossstacked structures on the sensing characteristics were systemati-cally investigated.The flexible strain sensor could capture low detection limit(<0.1%)with a gauge factor(GF)of 4.24 and wide strain range up to 130%.The uniform GF value reached 2050 when the strain was in the range of 100-130%.In addition,the high linearity under 40%strain(>0.998),excellent durability and quick response time(<200 ms)were demonstrated.The excellent com-prehensive performances were simultaneously obtained.The sen-sor could be used in extensive applications,such as monitoring body movements and distinguishing the track of writing.展开更多
Previous studies have indicated an association of fat mass and obesity-associated(FTO)with nonalcoholic fatty liver disease(NAFLD),the most common chronic liver disease worldwide.This study aimed to decipher the compl...Previous studies have indicated an association of fat mass and obesity-associated(FTO)with nonalcoholic fatty liver disease(NAFLD),the most common chronic liver disease worldwide.This study aimed to decipher the complex role of FTO in hepatic lipid metabolism.We found that a decrease in N^(6)-methyladenosine(m^(6)A)RNA methylation in the liver of mice fed with a high-fat diet(HFD)was accompanied by an increase in FTO expression.Overexpression of FTO in the liver promoted triglyceride accumulation by upregulating the expression of lipogenic genes.Mechanistical studies revealed that FTO could stabilize the mRNAs of sterol regulatory element binding transcription factor 1(SREBF1)and carbohydrate responsive element binding protein(ChREBP),two master lipogenic transcription factors,by demethylating m^(6)A sites.Knockdown ofeither SREBF1 or ChREBP attenuated the lipogenic effect of FTO,suggesting that they are bona fide effectors for FTO in regulating lipogenesis.Insulin could stimulate FTO transcription through a mechanism involving the action of intranuclear insulin receptor beta,while knockdown of FTO abrogated the lipogenic effect of insulin.Inhibition ofFTo by entacapone decreased the expression of SREBF1,ChREBP,and downstream lipogenic genes,ameliorating liver steatosis in HFD-fed mice.Thus,our study established a critical role of FTO in both the insulin-regulated hepatic lipogenesis and the pathogenesis of NAFLD and provided a potential strategyfor treating NAFLD.展开更多
Entity Linking(EL)aims to automatically link the mentions in unstructured documents to corresponding entities in a knowledge base(KB),which has recently been dominated by global models.Although many global EL methods ...Entity Linking(EL)aims to automatically link the mentions in unstructured documents to corresponding entities in a knowledge base(KB),which has recently been dominated by global models.Although many global EL methods attempt to model the topical coherence among all linked entities,most of them failed in exploiting the correlations among manifold knowledge helpful for linking,such as the semantics of mentions and their candidates,the neighborhood information of candidate entities in KB and the fine-grained type information of entities.As we will show in the paper,interactions among these types of information are very useful for better characterizing the topic features of entities and more accurately estimating the topical coherence among all the referred entities within the same document.In this paper,we present a novel HEterogeneous Graph-based Entity Linker(HEGEL)for global entity linking,which builds an informative heterogeneous graph for every document to collect various linking clues.Then HEGEL utilizes a novel heterogeneous graph neural network(HGNN)to integrate the different types of manifold information and model the interactions among them.Experiments on the standard benchmark datasets demonstrate that HEGEL can well capture the global coherence and outperforms the prior state-of-the-art EL methods.展开更多
Utilizing sunlight to split water into H_(2) and O_(2) is a highly promising approach in renewable energy production approaches.Recently,significant efforts have been devoted to developing innovative photocatalysts fo...Utilizing sunlight to split water into H_(2) and O_(2) is a highly promising approach in renewable energy production approaches.Recently,significant efforts have been devoted to developing innovative photocatalysts for splitting water.Metal-free two-dimensional(2D)covalent organic frameworks(COFs)are emerging as ideal catalytic platforms for this purpose.However,the rational design of these materials requires appropriate band alignment and active sites capable of catalyzing both hydrogen and oxygen evolution reactions(OERs),which depends on the judicious selection of molecular precursors.To address these requirements,first-principles calculations have proven to be an efficient method for designing and screening potential photocatalysts.Here,we provide a concise overview of recent advancements in the development of 2D COFs photocatalysts for overall water splitting(OWS),examining it from a theoretical perspective.This includes outlining the design principles,exploring the data-driven discovery of potential candidates using a COFs database,and applying machine learning(ML)tech-niques to predict the electronic structure of COFs based on the molecular orbitals of their precursors.Furthermore,we discuss the accuracy of current computational methods and address future challenges and potential of 2D COFs in practical applications for OWS.展开更多
Background:Liver cancer is largely resistant to chemotherapy.This study aimed to identify the effective chemotherapeutics forβ-catenin-activated liver cancer which is caused by gain-of-function mutation of catenin be...Background:Liver cancer is largely resistant to chemotherapy.This study aimed to identify the effective chemotherapeutics forβ-catenin-activated liver cancer which is caused by gain-of-function mutation of catenin beta 1(CTNNB1),the most frequently altered proto-oncogene in hepatic neoplasms.Methods:Constitutiveβ-catenin-activated mouse embryonic fibroblasts(MEFs)were established by deleting exon 3(β-catenin^(Δ(ex3)/+)),the most common mutation site in CTNNB1 gene.A screening of 12 widely used chemotherapy drugs was conducted for the ones that selectively inhibitedβ-catenin^(Δ(ex3)/+)but not for wild-type MEFs.Untargeted metabolomics was carried out to examine the alterations of metabolites in nucleotide synthesis.The efficacy and selectivity of methotrexate(MTX)onβ-catenin-activated human liver cancer cells were determined in vitro.Immuno-deficient nude mice subcutaneously inoculated withβ-catenin wild-type or mutant liver cancer cells and hepatitis B virus(HBV);β-catenin^(lox(ex3)/+)mice were used,respectively,to evaluate the efficacy of MTX in the treatment ofβ-catenin mutant liver cancer.Results:MTX was identified and validated as a preferential agent against the proliferation and tumor formation ofβ-catenin-activated cells.Boosted nucleotide synthesis was the major metabolic aberration inβ-catenin-active cells,and this alteration was also the target of MTX.Moreover,MTX abrogated hepatocarcinogenesis of HBV;β-catenin^(lox(ex3)/+)mice,which stimulated concurrent Ctnnb1-activated mutation and HBV infection in liver cancer.Conclusion:MTX is a promising chemotherapeutic agent forβ-catenin hyperactive liver cancer.Since repurposing MTX has the advantages of lower risk,shorter timelines,and less investment in drug discovery and development,a clinical trial is warranted to test its efficacy in the treatment ofβ-catenin mutant liver cancer.展开更多
The on-demand food delivery(OFD)service has gained rapid development in the past decades but meanwhile encounters challenges for further improving operation quality.The order dispatching problem is one of the most con...The on-demand food delivery(OFD)service has gained rapid development in the past decades but meanwhile encounters challenges for further improving operation quality.The order dispatching problem is one of the most concerning issues for the OFD platforms,which refer to dynamically dispatching a large number of orders to riders reasonably in very limited decision time.To solve such a challenging combinatorial optimization problem,an effective matching algorithm is proposed by fusing the reinforcement learning technique and the optimization method.First,to deal with the large-scale complexity,a decoupling method is designed by reducing the matching space between new orders and riders.Second,to overcome the high dynamism and satisfy the stringent requirements on decision time,a reinforcement learning based dispatching heuristic is presented.To be specific,a sequence-to-sequence neural network is constructed based on the problem characteristic to generate an order priority sequence.Besides,a training approach is specially designed to improve learning performance.Furthermore,a greedy heuristic is employed to effectively dispatch new orders according to the order priority sequence.On real-world datasets,numerical experiments are conducted to validate the effectiveness of the proposed algorithm.Statistical results show that the proposed algorithm can effectively solve the problem by improving delivery efficiency and maintaining customer satisfaction.展开更多
Quantitative analysis is essential for virus research,especially in determining the virus titer.The classical method plaque assay is time-consuming,complex,and difficult for the phages that cannot form apparent plaque...Quantitative analysis is essential for virus research,especially in determining the virus titer.The classical method plaque assay is time-consuming,complex,and difficult for the phages that cannot form apparent plaque on the solid medium.In order to realize rapid and effective detection,a new method combining atomic force microscopy(AFM)observation and mathematical calculation is established.In this research,M13 phages with an appropriate dilution ratio were observed and counted by AFM.Based on the counting results,the titer of M13 phages can be calculated simply through mathematical substitution.Instead of cultivating overnight in plaque assay,this new method can be implemented within a few hours.Moreover,it is a method that can achieve visualization for titer determination and have the potential to determine the phages that fail to form apparent plaque,which is significant in virus quantitative assessment.展开更多
基金supported by National Basic Research Program of China 973 Program (2015CB553802)the National Natural Science Foundation of China (81730078)Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (CIFMS2016-I2M-1-001)
文摘Golgi membrane protein 1(GOLM1/GP73)is a serum marker of hepatocellular carcinoma(HCC).We have previously shown that mTOR promoted tumorigenesis of HCC through stimulating GOLM1 expression.In this study,we demonstrated that the mammalian target of rapamycin(mTOR)was a negative regulator of microRNA-145(miR-145)expression.miR-145 inhibited GOLM1 expression by targeting a coding sequence of GOLM1 gene.GOLM1 and miR-145 were inversely correlated in human HCC tissues.GOLM1-enriched exosomes activated the glycogen synthase kinase-3β/matrix metalloproteinases(GSK-3β/MMPs)signaling axis of recipient cells and accelerated cell proliferation and migration.In contrast,miR-145 suppressed tumorigenesis and metastasis.We suggest that mTOR/miR-145/GOLM1 signaling pathway should be targeted for HCC treatment.
基金supported financially by the National Natural Science Foundation of China(Nos.51474156,U1660201)the National High Technology Research and Development Program of China(No.2015AA042504)。
文摘Recently,theγ’-strengthened superalloys are of great interests in high temperature applications due to their excellent high temperature strength which is derived from theγ’strengthening phase.For theseγ’-strengthened superalloys,the changes in morphology,size and distribution ofγ’precipitates due to coarsening during thermal exposure have a significant impact on the properties of alloys.This article briefly summarizes the recent advances on the coarsening behavior of gamma prime precipitates in the recently-developedγ’-strengthened superalloys and its effects on the hot deformation behavior of superalloys,drawing specific examples on Allvac^(■)718 Plus TM and Ni3 Al-based intermetallic superalloys.It is found that the particle size plays an important role in morphological evolution ofγ’precipitates.For instance,the morphology ofγ’precipitates evolves from cuboidal to strip-like or other complex structures in Ni3 Al-based intermetallic alloys,while theγ’precipitates in Allvac^(■)718 Plus alloy always present nearspherical morphology due to the relatively small initial particle size.The Lifshitz-Slyozof-Wagner(LSW)theory and its modifications,as well as Trans-Interface Diffusion Controlled(TIDC)theory have been applied to describing the coarsening kinetics ofγ’precipitates.Additionally,the hot deformation behavior ofγ’-strengthened superalloy is found to be greatly influenced by the coarsening ofγ’precipitates.
基金the National High Technology Research and Development Program(“863”Program)of China(2012AA021100)Ganpo 555 Talents Program of Jiangxi Province+2 种基金the Major Science and Technology Support Project from the Department of Science and Technology of Jiangxi Province(20132BAB205007)the Science and Technology Floor Project from the Department of Education of Jiangxi Province(KJLD12044)the Science and Technology Program from the Department of Health of Jiangxi Province(20121095).
文摘Objective:To describe the Chinese experience of natural orifice transluminal endoscopic surgery(NOTES)in urology.Methods:From December 2008 to May 2017,35 animal experiments and 305 clinical surgeries of NOTES or natural orifices specimen extractions(NOSE)were performed in China.The animal experiments included five kidney biopsies,24 nephrectomies and six partial nephrectomies.The clinical surgeries included 12 transvaginal NOSE(TV-NOSE),266 hybrid transvaginal NOTES(TV-NOTES)and 27 pure TV-NOTES.The TV-NOSE procedure was performed in five transumbilical laparoendoscopic single-site(U-LESS)nephrectomies,four suprapubic-assisted laparoendoscopic single-site surgery(SA-LESS)nephroureterectomies,and three laparoscopic radical cystectomies.The hybrid TV-NOTES procedure included 210 nephrectomies,31 adrenalectomies,eight nephroureterectomies,13 partial nephrectomies,and four heminephrectomies.The pure TV-NOTES procedure included five renal cyst decortications and 22 nephrectomies.Results:A total of 29 animal experiments were successfully performed.One partial nephrectomy was converted to standard laparoscopic surgery.Two kidney biopsies and two nephrectomies were unsuccessful.A total of 297 clinical surgeries were successfully performed.Six patients who underwent hybrid TV-NOTES were converted to open surgery.Two patients who underwent pure TV-NOTES were converted to SA-LESS.There were 22 major complications,16 occurred intraoperatively and six postoperatively.The mean visual analog score(VAS)of 48 h after the operation was 2.5 points in TV-NOSE,2.3 points in hybrid TV-NOTES and 1.7 points in pure TV-NOTES.The mean follow-up of 50.6(3.0-87.0)months showed that all patients were in good condition.The umbilicus scars were nearly invisible in TV-NOSE and hybrid TV-NOTES.The vaginal incision healed well.Conclusions:TV-NOSE and TV-NOTES are feasible,safe,and effective with little injury,low pain,fast recovery,and good cosmetic outcomes in properly selected patients.They are worth consideration for urological clinical practice.
基金This work is supported by the Aeronautical Science Foundation of China under Grant 20165515001the National Natural Science Foundation of China under Grant No.61402225State Key Laboratory for smart grid protection and operation control Foundation,and the Science and Technology Funds from National State Grid Ltd.(The Research on Key Technologies of Distributed Parallel Database Storage and Processing based on Big Data).
文摘Tiered Mobile Wireless Sensor Network(TMWSN)is a new paradigm introduced by mobile edge computing.Now it has received wide attention because of its high scalability,robustness,deployment flexibility,and it has a wide range of application scenarios.In TMWSNs,the storage nodes are the key nodes of the network and are more easily captured and utilized by attackers.Once the storage nodes are captured by the attackers,the data stored on them will be exposed.Moreover,the query process and results will not be trusted any more.This paper mainly studies the secure KNN query technology in TMWSNs,and we propose a secure KNN query algorithm named the Basic Algorithm For Secure KNN Query(BAFSKQ)first,which can protect privacy and verify the integrity of query results.However,this algorithm has a large communication overhead in most cases.In order to solve this problem,we propose an improved algorithm named the Secure KNN Query Algorithm Based on MR-Tree(SEKQAM).The MR-Trees are used to find the K-nearest locations and help to generate a verification set to process the verification of query results.It can be proved that our algorithms can effectively guarantee the privacy of the data stored on the storage nodes and the integrity of the query results.Our experimental results also show that after introducing the MR-Trees in KNN queries on TMWSNs,the communication overhead has an effective reduction compared to BAFSKQ.
基金supported by the National Natural Science Foundation of China (Grant Nos. 42075028 and 42222502)the Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Grant SML2021SP302)
文摘The Ross-Amundsen sector is experiencing an accelerating warming trend and a more intensive advective influx of marine air streams.As a result,massive surface melting events of the ice shelf are occurring more frequently,which puts the West Antarctica Ice Sheet at greater risk of degradation.This study shows the connection between surface melting and the prominent intrusion of warm and humid air flows from lower latitudes.By applying the Climate Feedback-Response Analysis Method(CFRAM),the temporal surge of the downward longwave(LW)fluxes over the surface of the Ross Ice Shelf(RIS)and adjacent regions are identified for four historically massive RIS surface melting events.The melting events are decomposed to identify which physical mechanisms are the main contributors.We found that intrusions of warm and humid airflow from lower latitudes are conducive to warm air temperature and water vapor anomalies,as well as cloud development.These changes exert a combined impact on the abnormal enhancement of the downward LW surface radiative fluxes,significantly contributing to surface warming and the resultant massive melting of ice.
基金National Key R&D program of China,Grant/Award Number:2021YFC2500700The National Natural Science Foundation of China+1 种基金Grant/Award Number:81730078The Chinese Academy of Medical Sciences Initiative for Innovative Medicine,Grant/Award Number:2021-I2M-1-049。
文摘Background:The expression of pyruvate kinase muscle 2(PKM2)is augmented in macrophages of patients with atherosclerotic coronary artery disease.The role of PKM2 in atherosclerosis is to be determined.Methods:Global and myeloid cell-specific PKM2 knock-in mice with ApoE^(-/-)background(ApoE^(-/-),PKM2^(KI/KI)and Lyz2-cre,ApoE^(-/-),and PKM2^(flox/flox))were produced to evaluate the clinical significance of PKM2 in atherosclerosis development.Wild-type and PKM2 knock-in macrophages were isolated to assess the function of PKM2 in macrophage phagocytosis.Atherosclerotic mice were treated with PKM2 inhibitor shikonin(SKN)to evaluate the therapeutic potential of PKM2 suppression in atherosclerosis.Results:Oxidized low-density lipoprotein(oxLDL)upregulated PKM2 in macrophages.PKM2 in return promoted the uptake of oxLDL by macrophages.Overexpressed PKM2 accelerated atherosclerosis in mice.SKN blocked the progress of mouse atherosclerosis.Conclusions:PKM2 accelerates macrophage phagocytosis and atherosclerosis.Targeting PKM2 is a potential therapy for atherosclerosis.
基金jointly supported by the National Natural Science Foundation of China [grant numbers 4208810141911540470+3 种基金42075028]the Guangdong Major Project of Basic and Applied Basic Research [grant number 2020B0301030004]the Natural Science Foundation of Guangdong Province of China [grant number 2018A0303130268]the Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies [grant number2020B1212060025]。
文摘Driven by the increase in CO_(2)concentration,climate models reach a consensus that the large-scale circulation of the South Asian summer monsoon(SASM) becomes weakened but with different magnitudes.This study investigates the major uncertainty sources of the SASM response to an abrupt quadrupling of CO_(2)(abrupt-4×CO_(2))in 18 models of phase 6 of the Coupled Model Intercomparison Project.The projected weakening of the SASM indicated by both zonal and meridional monsoon circulation indices is closely linked to decreases in the meridional gradient of upper-tropospheric temperature between Eurasia and the Indian Ocean(EUTT-IUTT).A climate feedback-response analysis method is applied to linearly decompose the uncertainty of changes in EUTT-IUTT into the partial changes due to external forcing and internal processes of the earth-atmosphere column.Results show that the uncertainty of changes in EUTT-IUTT is contributed positively by the dominant atmospheric dynamic process,followed by the cloud shortwave radiative effect,and negatively by the surface latent heat flux and cloud longwave radiative effect.Contributions from CO_(2)forcing and other internal processes including albedo and water vapor feedbacks,oceanic heat storage,and sensible heat flux are found to be minor.
文摘Based on the traditional Human-Computer Interaction method which is mainly touch input system, the way of capturing the movement of people by using cameras is proposed. This is a convenient technique which can provide users more experience. In the article, a new way of detecting moving things is given on the basis of development of the image processing technique. The system architecture decides that the communication should be used between two different applications. After considered, named pipe is selected from many ways of communication to make sure that video is keeping in step with the movement from the analysis of the people moving. According to a large amount of data and principal knowledge, thinking of the need of actual project, a detailed system design and realization is finished. The system consists of three important modules: detecting of the people's movement, information transition between applications and video showing in step with people's movement. The article introduces the idea of each module and technique.
文摘Since the 1980s,educational experts and scholars from western countries have carried out multi-level and multi-angle research on“reflection,”“reflective teaching,”“reflective teacher,”and other subject matters,and fruitful results have been achieved[1].Since the late 1990s,the research on reflective teachers in China has gradually deepened.Not only a considerable number of papers have been published,but also numerous monographs.This paper defines the term“reflective teacher,”explores and studies the nurturing of reflective teachers,clarifies the relationship between reflection and classroom,and highlights the characteristics that reflective teachers should have.
基金supported by the Science and Technology Guidance Project of China National Textile and Apparel Council (Grant No.2024033)the Jiangsu Provincial Key Research and Development Program (Grant No.BE2019045)。
文摘The development of multifunctional intelligent textiles has become an important innovation direction in the field of textile engineering, under the dual demands of intelligent health monitoring and environmental protection. Although singlefunctional textiles with antibacterial, photochromic, and strain-sensing properties have been developed, they are unable to meet the demand for multifunctional textiles. In this respect, this study developed a poly(lactic acid) and poly(3-hydroxybutyrate)/thermoplastic polyurethane/carbon black nanoparticle composite nanofiber yarn(PPTCY) with a hollow-core-sheath structure using a simple conjugate electrospinning technology. PPTCY possessed excellent mechanical strength and could be effectively woven. More importantly, it not only enabled real-time visual monitoring of ultraviolet(UV) intensity in the environment but also possessed excellent antibacterial properties and strain-sensing performance. Its ΔE value was up to 58.24, and its antibacterial rates against Escherichia coli and Staphylococcus aureus were both 99.99%. This fabric had excellent strainsensing performance, high linearity, and durability under both pressure and stretching deformations. This research provides favorable technical support for the application of intelligent textiles in the field of UV protection and traffic safety.
基金This work was supported by grants from the National Natural Science Foundation of China(81570768 to Y.-C.W.,31900841 to Yan Li,91957205 and 31525012 to H.Y.,and 81471016 to J.J.)the Ministry of Science and Technology of China(2016YFA0500102 and 2016YFC1304905)CAS Key Laboratory of Nutrition,Metabolism and Food Safety(KLNMFS2019-01),and Chinese Academy of Sciences Interdisciplinary Innovation Team.
文摘Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation.A growing body of evidence suggests that microRNAs play critical roles in myocyte proliferation and differentiation transcriptionally.However,the molecular mechanisms underlying the orchestration are not fully understood.Here,we showed that miR-130b is able to repress myoblast proliferation and promote myogenic differentiation via targeting Sp1 transcription factor.Importantly,overexpression of miR-130b is capable of improving the recovery of damaged muscle in a freeze injury model.Moreover,miR-130b expression is declined in the muscle of muscular dystrophy patients.Thus,these results indicated that miR-130b may play a role in skeletal muscle regeneration and myopathy progression.Together,our findings suggest that the miR-130b/Sp1 axis may serve as a potential therapeutic target for the treatment of patients with muscle damage or severe myopathies.
基金This work was supported by the Jiangsu Planned Projects for Postdoctoral Research Funds[2020Z251]Primary Research&Developement Plan of Jiangsu Province[BE2019045]+1 种基金Science and Technology Guidance Project of China National Textile and Apparel Council[2020102]Third-Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘Carbon nanofibers(CNFs)with excellent conductivity and stability have become a promising material to design the strain sensing network.To date,however,the effect of the stacked structure of CNF membrane on the sensing performance has rarely been stu-died.In this work,we reported a high-performance sensor based on the cross-stacked aligned CNF membrane.The effects of crossstacked structures on the sensing characteristics were systemati-cally investigated.The flexible strain sensor could capture low detection limit(<0.1%)with a gauge factor(GF)of 4.24 and wide strain range up to 130%.The uniform GF value reached 2050 when the strain was in the range of 100-130%.In addition,the high linearity under 40%strain(>0.998),excellent durability and quick response time(<200 ms)were demonstrated.The excellent com-prehensive performances were simultaneously obtained.The sen-sor could be used in extensive applications,such as monitoring body movements and distinguishing the track of writing.
基金This work was supported by the Chinese Ministry of Science and Technology(2021YFA1100500)the National Natural Science Foundation of China(NSFC,91957205,82070821,and 82000805)+4 种基金Youth Innovation Promotion Association,Chinese Academy of Sciences(2021261)Pujiang Talent Program from the Science and Technology Commission of Shanghai Municipality(21PJ1416100)Young Elite Scientists Sponsorship Program by China Association for Science and Technology(2020QNRC001)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Jiangnan University(2022-3-1)NHC Key Laboratory of Food Safety Risk Assessment(2020K02).
文摘Previous studies have indicated an association of fat mass and obesity-associated(FTO)with nonalcoholic fatty liver disease(NAFLD),the most common chronic liver disease worldwide.This study aimed to decipher the complex role of FTO in hepatic lipid metabolism.We found that a decrease in N^(6)-methyladenosine(m^(6)A)RNA methylation in the liver of mice fed with a high-fat diet(HFD)was accompanied by an increase in FTO expression.Overexpression of FTO in the liver promoted triglyceride accumulation by upregulating the expression of lipogenic genes.Mechanistical studies revealed that FTO could stabilize the mRNAs of sterol regulatory element binding transcription factor 1(SREBF1)and carbohydrate responsive element binding protein(ChREBP),two master lipogenic transcription factors,by demethylating m^(6)A sites.Knockdown ofeither SREBF1 or ChREBP attenuated the lipogenic effect of FTO,suggesting that they are bona fide effectors for FTO in regulating lipogenesis.Insulin could stimulate FTO transcription through a mechanism involving the action of intranuclear insulin receptor beta,while knockdown of FTO abrogated the lipogenic effect of insulin.Inhibition ofFTo by entacapone decreased the expression of SREBF1,ChREBP,and downstream lipogenic genes,ameliorating liver steatosis in HFD-fed mice.Thus,our study established a critical role of FTO in both the insulin-regulated hepatic lipogenesis and the pathogenesis of NAFLD and provided a potential strategyfor treating NAFLD.
基金supported in part by the National Key R&D Program of China(No.2020AAA0106600)the Key Laboratory of Science,Technology and Standard in Press Industry(Key Laboratory of Intelligent Press Media Technology)
文摘Entity Linking(EL)aims to automatically link the mentions in unstructured documents to corresponding entities in a knowledge base(KB),which has recently been dominated by global models.Although many global EL methods attempt to model the topical coherence among all linked entities,most of them failed in exploiting the correlations among manifold knowledge helpful for linking,such as the semantics of mentions and their candidates,the neighborhood information of candidate entities in KB and the fine-grained type information of entities.As we will show in the paper,interactions among these types of information are very useful for better characterizing the topic features of entities and more accurately estimating the topical coherence among all the referred entities within the same document.In this paper,we present a novel HEterogeneous Graph-based Entity Linker(HEGEL)for global entity linking,which builds an informative heterogeneous graph for every document to collect various linking clues.Then HEGEL utilizes a novel heterogeneous graph neural network(HGNN)to integrate the different types of manifold information and model the interactions among them.Experiments on the standard benchmark datasets demonstrate that HEGEL can well capture the global coherence and outperforms the prior state-of-the-art EL methods.
基金support from the National Natural Science Foundation of China(22073807,22225301)the CAS Project for Young Scientists in Basic Research(YSBR-004)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0450101)the Fundamental Research Funds for the Central Universities(20720220007)the support from Super Computer Centre of USTCSCC and SCCAS.
文摘Utilizing sunlight to split water into H_(2) and O_(2) is a highly promising approach in renewable energy production approaches.Recently,significant efforts have been devoted to developing innovative photocatalysts for splitting water.Metal-free two-dimensional(2D)covalent organic frameworks(COFs)are emerging as ideal catalytic platforms for this purpose.However,the rational design of these materials requires appropriate band alignment and active sites capable of catalyzing both hydrogen and oxygen evolution reactions(OERs),which depends on the judicious selection of molecular precursors.To address these requirements,first-principles calculations have proven to be an efficient method for designing and screening potential photocatalysts.Here,we provide a concise overview of recent advancements in the development of 2D COFs photocatalysts for overall water splitting(OWS),examining it from a theoretical perspective.This includes outlining the design principles,exploring the data-driven discovery of potential candidates using a COFs database,and applying machine learning(ML)tech-niques to predict the electronic structure of COFs based on the molecular orbitals of their precursors.Furthermore,we discuss the accuracy of current computational methods and address future challenges and potential of 2D COFs in practical applications for OWS.
基金supported by grants from the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(No.2021-1-I2M-018)the Haihe Laboratory of Cell Ecosystem Innovation Fund(No.22HHXBSS00012)the National Natural Science Foundation of China(Nos.81730078 and 81872287)
文摘Background:Liver cancer is largely resistant to chemotherapy.This study aimed to identify the effective chemotherapeutics forβ-catenin-activated liver cancer which is caused by gain-of-function mutation of catenin beta 1(CTNNB1),the most frequently altered proto-oncogene in hepatic neoplasms.Methods:Constitutiveβ-catenin-activated mouse embryonic fibroblasts(MEFs)were established by deleting exon 3(β-catenin^(Δ(ex3)/+)),the most common mutation site in CTNNB1 gene.A screening of 12 widely used chemotherapy drugs was conducted for the ones that selectively inhibitedβ-catenin^(Δ(ex3)/+)but not for wild-type MEFs.Untargeted metabolomics was carried out to examine the alterations of metabolites in nucleotide synthesis.The efficacy and selectivity of methotrexate(MTX)onβ-catenin-activated human liver cancer cells were determined in vitro.Immuno-deficient nude mice subcutaneously inoculated withβ-catenin wild-type or mutant liver cancer cells and hepatitis B virus(HBV);β-catenin^(lox(ex3)/+)mice were used,respectively,to evaluate the efficacy of MTX in the treatment ofβ-catenin mutant liver cancer.Results:MTX was identified and validated as a preferential agent against the proliferation and tumor formation ofβ-catenin-activated cells.Boosted nucleotide synthesis was the major metabolic aberration inβ-catenin-active cells,and this alteration was also the target of MTX.Moreover,MTX abrogated hepatocarcinogenesis of HBV;β-catenin^(lox(ex3)/+)mice,which stimulated concurrent Ctnnb1-activated mutation and HBV infection in liver cancer.Conclusion:MTX is a promising chemotherapeutic agent forβ-catenin hyperactive liver cancer.Since repurposing MTX has the advantages of lower risk,shorter timelines,and less investment in drug discovery and development,a clinical trial is warranted to test its efficacy in the treatment ofβ-catenin mutant liver cancer.
基金supported in part by the National Natural Science Foundation of China(No.62273193)Tsinghua University-Meituan Joint Institute for Digital Life,and the Research and Development Project of CRSC Research&Design Institute Group Co.,Ltd.
文摘The on-demand food delivery(OFD)service has gained rapid development in the past decades but meanwhile encounters challenges for further improving operation quality.The order dispatching problem is one of the most concerning issues for the OFD platforms,which refer to dynamically dispatching a large number of orders to riders reasonably in very limited decision time.To solve such a challenging combinatorial optimization problem,an effective matching algorithm is proposed by fusing the reinforcement learning technique and the optimization method.First,to deal with the large-scale complexity,a decoupling method is designed by reducing the matching space between new orders and riders.Second,to overcome the high dynamism and satisfy the stringent requirements on decision time,a reinforcement learning based dispatching heuristic is presented.To be specific,a sequence-to-sequence neural network is constructed based on the problem characteristic to generate an order priority sequence.Besides,a training approach is specially designed to improve learning performance.Furthermore,a greedy heuristic is employed to effectively dispatch new orders according to the order priority sequence.On real-world datasets,numerical experiments are conducted to validate the effectiveness of the proposed algorithm.Statistical results show that the proposed algorithm can effectively solve the problem by improving delivery efficiency and maintaining customer satisfaction.
基金We thank the Foundation of State Key Laboratory of NBC Protection for Civilian SKLNBC2018-03 and SKLNBC2020-02 for financial support.
文摘Quantitative analysis is essential for virus research,especially in determining the virus titer.The classical method plaque assay is time-consuming,complex,and difficult for the phages that cannot form apparent plaque on the solid medium.In order to realize rapid and effective detection,a new method combining atomic force microscopy(AFM)observation and mathematical calculation is established.In this research,M13 phages with an appropriate dilution ratio were observed and counted by AFM.Based on the counting results,the titer of M13 phages can be calculated simply through mathematical substitution.Instead of cultivating overnight in plaque assay,this new method can be implemented within a few hours.Moreover,it is a method that can achieve visualization for titer determination and have the potential to determine the phages that fail to form apparent plaque,which is significant in virus quantitative assessment.
