The basic leucine zipper(bZIP)transcription factor family,one of the largest and most diverse transcription factor families in eukaryotes,is associated with the development and stress responses of many eukaryotic orga...The basic leucine zipper(bZIP)transcription factor family,one of the largest and most diverse transcription factor families in eukaryotes,is associated with the development and stress responses of many eukaryotic organisms.However,their biological functions in oomycete plant pathogens are unclear.A genome-wide analysis of bZIP transcription factors in Phytophthora sojae showed that PsBZP32,which has a unique bZIP-PAS domain structure,exhibited a high transcription level during the early stages of P.sojae infection.We silenced PsBZP32 in P.sojae and found that the transformants showed defective cyst germination and pathogenicity.The transformants could not eliminate host-derived reactive oxygen species during infection and were more sensitive to oxidative stress.Their response to oxidative stress was independent of the PsBZP32 transcription level;however,subcellular localization and phosphorylation of PsBZP32 were affected by H_(2)O_(2)stress.These results indicate that PsBZP32 is involved in regulation of P.sojae cyst germination,oxidative stress response,and pathogenicity.展开更多
基金supported by the National Natural Science Foundation(31401688,31772140)to Wenwu Yethe National Natural Science Foundation(31721004)to Yuanchao Wang.
文摘The basic leucine zipper(bZIP)transcription factor family,one of the largest and most diverse transcription factor families in eukaryotes,is associated with the development and stress responses of many eukaryotic organisms.However,their biological functions in oomycete plant pathogens are unclear.A genome-wide analysis of bZIP transcription factors in Phytophthora sojae showed that PsBZP32,which has a unique bZIP-PAS domain structure,exhibited a high transcription level during the early stages of P.sojae infection.We silenced PsBZP32 in P.sojae and found that the transformants showed defective cyst germination and pathogenicity.The transformants could not eliminate host-derived reactive oxygen species during infection and were more sensitive to oxidative stress.Their response to oxidative stress was independent of the PsBZP32 transcription level;however,subcellular localization and phosphorylation of PsBZP32 were affected by H_(2)O_(2)stress.These results indicate that PsBZP32 is involved in regulation of P.sojae cyst germination,oxidative stress response,and pathogenicity.
