Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food safety.In this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined ...Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food safety.In this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)detection.Quantum dot nanobeads(QBs)served as fluorescence reporters,providing good detection efficiency.The customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product capture.Detection was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)line.The linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test strips.ST specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and SD-LAMP.We detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.展开更多
Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by pro...Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.展开更多
Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the p...Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the preparation of functional walnut peptide ingredients or additives and other compositions has received increasing attention.However,the improvement of the yield of walnut peptides and the clarification of their functional activities are the bottlenecks that limit the development of these peptides.To this end,this article reviews the pretreatment,preparation,purification and identification processes of walnut peptides,as well as their biological activities such as antioxidant activity,antitumour activity,improvement of memory,antihypertension and regulation of metabolic disorders are elaborated to provide a reference for the industrial development of walnut peptides.展开更多
Recent advances in deep learning have led to disruptive breakthroughs in artificial intelligence(AI),fueling the jump in ChatGPT-like large language models(LLMs).As with any emerging technology,it is a two-sided coin,...Recent advances in deep learning have led to disruptive breakthroughs in artificial intelligence(AI),fueling the jump in ChatGPT-like large language models(LLMs).As with any emerging technology,it is a two-sided coin,bringing not only vast social impacts but also significant security concerns,especially in the socio-cognitive domain.Against this back-ground,this work starts with an inherent mechanism analysis of cognitive domain games,from which it proceeds to explore the security concerns facing the cognitive domain as well as to analyze the formation mechanisms of a cognitive immune system.Finally,inspired by behavioral mimicry in biology,this work will elaborate on new approaches to cognitive security from three aspects:Mimicry Computing,Mimicry Defense,and Mimicry Intelligence.展开更多
To address the serious imbalance between the supply and demand of the cybersecurity workforce, this paper proposes to embrace the latest trend of a fundamental shift in the“underlying dynamics of the digital ecosyste...To address the serious imbalance between the supply and demand of the cybersecurity workforce, this paper proposes to embrace the latest trend of a fundamental shift in the“underlying dynamics of the digital ecosystem”, focusing on a shared liability for cybersecurity between the application side and the manufacturing side. Assuming that product providers shall take more responsibility by implementing secure defaults, this paper explores the establishment of an S&S talent cultivation system to strike the right balance of cybersecurity liabilities by nurturing more responsible developers. This paper proposes a Knowledge, Skill, and Awareness(KSA) model for Security and Safety(S&S) talent cultivation, proves the feasibility of this model by analyzing the theoretical, disciplinary, methodological, practical, and societal foundations of S&S talent cultivation. Additionally, this paper proposes principles and strategies for building a S&S talent cultivation system based on its unique characteristics and patterns. It gives a talent cultivation scheme, supported by an “Independent Knowledge System, Education and Cultivation System, Practice and Training system, Evaluation and Certification system,and Awareness Popularization System”. Finally, this paper puts forward a proposal for coordinating efforts and adopting multiple measures to accelerate the cultivation of S&S talents.展开更多
基金This work was supported by the National Key Research and Development Program of China(2019YFC1606300)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01S174)the Guangdong Academy of Sciences Special Project of Implementing Innovation-Driven Development Capacity Building(2018GDASCX-0401).
文摘Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food safety.In this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)detection.Quantum dot nanobeads(QBs)served as fluorescence reporters,providing good detection efficiency.The customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product capture.Detection was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)line.The linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test strips.ST specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and SD-LAMP.We detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.
基金supported financially by“Kunlun Talents High-end Innovation and Entrepreneurship Talents”of Qinghai Province in 2022National Natural Science Foundation of China(Nos.22322401 and 82073816)Beijing Nova Program(No.20220484055)。
文摘Foods are often contaminated by multiple foodborne pathogens,which threatens human health.In this work,we developed a microfluidic biosensor for multiplex immunoassay of foodborne bacteria with agitation driven by programmed audio signals.This agitation,powered by the vibration of a speaker cone during music playing,accelerated the mass transport in the incubation process to form bacterial complexes within 10 min.Immunoassay reagents of the two target bacteria(Escherichia coli O157:H7 and Salmonella typhimurium)were preloaded into the corresponding fore-vacuum storage chamber on the chip,and released to participate in the subsequent immune analysis process by piercing the chambers.All the detection processes were integrated into a single microfluidic chip and controlled by a smartphone through Bluetooth.Under selected conditions,wide linear ranges and low limits of detection(LODs<2CFU/m L)were obtained,and real food samples were successfully determined within 30 min.This biosensing method can be extended to wide-ranging applications by loading different recognizing reagents.
基金supports from the National Key Research and Development Program of China(CN)(2022YFD1600402)Hebei Provincial Major Science and Technology Achievement Transformation Project(CN)(21287101Z)+1 种基金Hebei Provincial Innovation and Entrepreneurship Team Project(CN)(215A7102D)Discipline Construction-Food Science and Engineering(CN)(SPCX-2022005).
文摘Walnuts are rich in protein and are a high-quality plant protein resource.In recent years,with the growth of consumer demand for functional food and food for special medical purpose,the use of walnut protein for the preparation of functional walnut peptide ingredients or additives and other compositions has received increasing attention.However,the improvement of the yield of walnut peptides and the clarification of their functional activities are the bottlenecks that limit the development of these peptides.To this end,this article reviews the pretreatment,preparation,purification and identification processes of walnut peptides,as well as their biological activities such as antioxidant activity,antitumour activity,improvement of memory,antihypertension and regulation of metabolic disorders are elaborated to provide a reference for the industrial development of walnut peptides.
基金supported in part by National Key R&D Plan(2022YFB3102901)
文摘Recent advances in deep learning have led to disruptive breakthroughs in artificial intelligence(AI),fueling the jump in ChatGPT-like large language models(LLMs).As with any emerging technology,it is a two-sided coin,bringing not only vast social impacts but also significant security concerns,especially in the socio-cognitive domain.Against this back-ground,this work starts with an inherent mechanism analysis of cognitive domain games,from which it proceeds to explore the security concerns facing the cognitive domain as well as to analyze the formation mechanisms of a cognitive immune system.Finally,inspired by behavioral mimicry in biology,this work will elaborate on new approaches to cognitive security from three aspects:Mimicry Computing,Mimicry Defense,and Mimicry Intelligence.
基金supported by the Chinese Academy of Engineering Strategic Research and Consulting Program (No. 2023-XZ-11)
文摘To address the serious imbalance between the supply and demand of the cybersecurity workforce, this paper proposes to embrace the latest trend of a fundamental shift in the“underlying dynamics of the digital ecosystem”, focusing on a shared liability for cybersecurity between the application side and the manufacturing side. Assuming that product providers shall take more responsibility by implementing secure defaults, this paper explores the establishment of an S&S talent cultivation system to strike the right balance of cybersecurity liabilities by nurturing more responsible developers. This paper proposes a Knowledge, Skill, and Awareness(KSA) model for Security and Safety(S&S) talent cultivation, proves the feasibility of this model by analyzing the theoretical, disciplinary, methodological, practical, and societal foundations of S&S talent cultivation. Additionally, this paper proposes principles and strategies for building a S&S talent cultivation system based on its unique characteristics and patterns. It gives a talent cultivation scheme, supported by an “Independent Knowledge System, Education and Cultivation System, Practice and Training system, Evaluation and Certification system,and Awareness Popularization System”. Finally, this paper puts forward a proposal for coordinating efforts and adopting multiple measures to accelerate the cultivation of S&S talents.
