The development of cost-effective and high-efficiency catalysts for sustainable hydrogen production through electrocatalytic hydrogen evolution reaction(HER) is crucial yet remains challenging. In this work, we synthe...The development of cost-effective and high-efficiency catalysts for sustainable hydrogen production through electrocatalytic hydrogen evolution reaction(HER) is crucial yet remains challenging. In this work, we synthesized two types of bimetallic Pt Ni nanoparticles embedded in N-doped porous carbons derived from Ni-ABDC(5-aminoisophthalate) using both in-situ and ex-situ Pt inclusion methods. The in-situ Pt doping notably disrupted the effective growth of Ni-ABDC nanostrips owing to strong interactions between Pt and ABDC, resulting in an amorphous nanostructure. The optimized PtinNi-NC exhibited remarkable HER performance with a low overpotential of 29 mV at 10 mA/cm^(2), a Tafel slope of 47.4 mV/dec, and a current retention of 91.2% after 200 h in 1.0 mol/L KOH solution, surpassing the performance of Ni-NC, PtexNi-NC, and Pt/C. This research demonstrates the rational design and preparation of transition metal-based coordination polymer-derived metal-carbon nanomaterials with low Pt loading,emphasizing their considerable potential in energy conversion and storage technologies.展开更多
Ozone(O_(3))pollution has a profound impact on human health,vegetation development,and the ecological environment,making it a critical focus of global academic research.In recent years,O_(3)pollution in China has been...Ozone(O_(3))pollution has a profound impact on human health,vegetation development,and the ecological environment,making it a critical focus of global academic research.In recent years,O_(3)pollution in China has been on a steady rise,with ozone emerging as the sole conventional pollutant to consistently increase in concentration without any decline.This study conducted a quantitative analysis of O_(3)concentrations across 367 Chinese cities in 2019,examining spatial autocorrelation and local clustering of O_(3)levels,and investigated the diverse relationships between human activity factors and O_(3)concentration.The seasonal fluctuation of O_(3)exhibited the“M-type”pattern,with peak concentrations in winter and the lowest levels in summer.The center of O_(3)pollution migrated southeastward,with the area of highest concentration progressively shifting south along the eastern coast.Moreover,O_(3)concentration showed a strong positive correlation with population density,road freight volume,and industrial emissions,suggesting that human activities,vehicle emissions,and industrial operations are significant contributors to O_(3)production.The results provide comprehensive information on the characteristics,causes,and occurrence mechanism of O_(3)in Chinese cities that can be utilized by global government departments to formulate strategies to prevent and control O_(3)pollution.展开更多
Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due...Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due to limitations in tumor antigen selection and delivery methods.Tumor vaccines often fail to elicit a sufficiently robust immune response against progressive tumors,thereby limiting their clinical efficacy.In this study,we developed a nanoparticle-based tumor vaccine,OVA@HA-PEI,utilizing ovalbumin(OVA)as the presenting antigen and hyaluronic acid(HA)and polyethyleneimine(PEI)as adjuvants and carriers.This formulation significantly enhanced the proliferation of immune cells and cytokines,such as CD3,CD8,interferon-,and tumor necrosis factor-,in vivo,effectively activating an immune response against B16–F10 tumors.In vivofluorescenceflow cytometry(IVFC)has already become an effective method for monitoring circulating tumor cells(CTCs)due to its direct,noninvasive,and long-term detection capabilities.Our study utilized a laboratory-constructed IVFC system to monitor the immune processes induced by the OVA@HA-PEI tumor vaccine and an anti-programmed death-1(PD-1)antibody.The results demonstrated that the combined treatment of OVA@HA-PEI and anti-PD-1 antibody significantly improved the survival time of mice compared to anti-PD-1 antibody treatment alone.Additionally,this combination therapy substantially reduced the number of CTCs in vivo,increased the clearance rate of CTCs by the immune system,and slowed tumor progression.Thesefindings greatly enhance the clinical application prospects of IVFC and tumor vaccines.展开更多
Due to the joint development characteristic and macropore structure of loess,it is easy to cause structure collapse under earthquake or artificial vibration.The study on the loess disaster effect and its mechanism und...Due to the joint development characteristic and macropore structure of loess,it is easy to cause structure collapse under earthquake or artificial vibration.The study on the loess disaster effect and its mechanism under earthquake action is insufficient due to its complexity.Hence,to study the deformation and mechanical properties more accurately,the dynamic characteristics of saturated remolded loess under cyclic dynamic load were tested using a GDS dynamic triaxial instrument in this paper.The test results show that strain and pore water pressure increase gradually at different rates with the development of vibration,and there is an obvious inflection point in the time-history curve of both.When the number of vibrations(N)exceeds this point,the strain increases rapidly,and pore water pressure tends to be stable.Under the action of large amplitude and low-frequency dynamic load,the strain and pore water pressure increase rapidly with fewer vibrations and the deviator stress(q)decreases rapidly,while the sample achieves damage faster with the increase of amplitude.During the application of a dynamic load,the effective stress(p)gradually decreases and its rate of change slows down.Finally,when the saturated remolded loess is subjected to a constant-amplitude dynamic load,the combination of large amplitude and low frequency leads to the failure of the sample in the shortest time.展开更多
The applications of single-cell and spatial technologies in recent times have revolutionized the present understanding of cellular states and the cellular heterogeneity inherent in complex biological systems.These adv...The applications of single-cell and spatial technologies in recent times have revolutionized the present understanding of cellular states and the cellular heterogeneity inherent in complex biological systems.These advancements offer unprecedented resolution in the examination of the functional genomics of individual cells and their spatial context within tissues.In this review,we have comprehensively discussed the historical development and recent progress in the field of single-cell and spatial genomics.We have reviewed the breakthroughs in single-cell multi-omics technologies,spatial genomics methods,and the computational strategies employed toward the analyses of singlecell atlas data.Furthermore,we have highlighted the advances made in constructing cellular atlases and their clinical applications,particularly in the context of disease.Finally,we have discussed the emerging trends,challenges,and opportunities in this rapidly evolving field.展开更多
Photobiomodulation,by utilizing low-power light in the visible and near-infrared spectra to trigger biological responses in cells and tissues,has been considered as a possible therapeutic strategy for Alzheimer’s dis...Photobiomodulation,by utilizing low-power light in the visible and near-infrared spectra to trigger biological responses in cells and tissues,has been considered as a possible therapeutic strategy for Alzheimer’s disease(AD),while its specific mechanisms have remained elusive.Here,we demonstrate that cognitive and memory impairment in an AD mouse model can be ameliorated by 1070-nm light via reducing cerebralβ-amyloid(Aβ)burden,the hallmark of AD.The glial cells,including microglia and astrocytes,play important roles in Aβclearance.Our results show that 1070-nm light pulsed at 10 Hz triggers microglia rather than astrocyte responses in AD mice.The 1070-nm lightinduced microglia responses with alteration in morphology and increased colocalization with Aβare sufficient to reduce Aβload in AD mice.Moreover,1070-nm light pulsed at 10 Hz can reduce perivascular microglia and promote angiogenesis to further enhance Aβclearance.Our study confirms the important roles of microglia and cerebral vessels in the use of 1070-nm light for the treatment of AD mice and provides a framework for developing a novel therapeutic approach for AD.展开更多
Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have r...Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have revealed that the circadian rhythm may disrupt the growth of tumors,it is generally unclear whether the circadian rhythm controls the release of CTCs.In clinical examinations,the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time,which is being challenged by recent studies.Since it is not practical to draw blood from patients repeatedly,a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods.Fluoresce nee in vivo flow cytometry(IVFC)is a powerful optical technique that is able to detect fluoresce nt circulating cells directly in living animals in a noninvasive manner over a long period of time.In this study,we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer.We observed that CTCs exhibited stochastic bursts over cancer progression.The probability of the bursting activity was higher at early stages than at late stages.We longitudinally monitored CTCs over a 24-h period,and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night(active phase for rodents),suggesting that the release of CTCs might be regulated by the circadian rhythm.展开更多
Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry i...Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry in a multiple myeloma mouse model in their previous work2 might be different from our results.Niedre et al.claimed that CTC detection statistics deviated from Poisson but did not found circadian variations in CTC numbers in a multiple myeloma mouse model.They also cite another literature by Juratli.et al.3,in which the authors reported that CTC numbers did not always correlate with tumor size during cancer progression.However,by establishing an orthotopic mouse model of prostate cancer and utilizing the technology in vivo flow cytometry(IVFC),we found CTCs exhibited bursting activity and daily oscillation in an orthotopic model of prostate cancer.展开更多
Mathematical modelling performs a vital part in estimating and controlling the recent outbreak of coronavirus disease 2019(COVID-19).In this epidemic,most countries impose severe intervention measures to contain the s...Mathematical modelling performs a vital part in estimating and controlling the recent outbreak of coronavirus disease 2019(COVID-19).In this epidemic,most countries impose severe intervention measures to contain the spread of COVID-19.The policymakers are forced to make difficult decisions to leverage between health and economic development.How and when tomake clinical and public health decisions in an epidemic situation is a challenging question.The most appropriate solution is based on scientific evidence,which is mainly dependent on data and models.So one of the most critical problems during this crisis is whether we can develop reliable epidemiological models to forecast the evolution of the virus and estimate the effectiveness of various intervention measures and their impacts on the economy.There are numerous types of mathematical model for epidemiological diseases.In this paper,we present some critical reviews on mathematical models for the outbreak of COVID-19.Some elementary models are presented as an initial formulation for an epidemic.We give some basic concepts,notations,and foundation for epidemiological modelling.More related works are also introduced and evaluated by considering epidemiological features such as disease tendency,latent effects,susceptibility,basic reproduction numbers,asymptomatic infections,herd immunity,and impact of the interventions.展开更多
Electrocatalytic water electrolysis is intrinsically limited by the slow kinetics of the oxygen evolution reaction(OER)at the anodic electrode.Building on our previous work,we utilized a porous metal-organic framework...Electrocatalytic water electrolysis is intrinsically limited by the slow kinetics of the oxygen evolution reaction(OER)at the anodic electrode.Building on our previous work,we utilized a porous metal-organic framework(CoOF-1)structurally characterized by rich adsorption sites for Ru(Ⅲ)ions.In this study,the incorporation of noble metal species into the CoOF-1-derived porous Co_(3)O_(4) matrix effectively improves electrocatalytic OER performance.展开更多
基金financially supported by National Natural Science Foundation of China (No. 21601137)Basic Science and Technology Research Project of Wenzhou, Zhejiang Province (No. G20240038)+2 种基金the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities Association (Nos. 202101BA070001-031, 202101BA070001-042 and 202301BA070001-093)Yunnan Province Young and Middle-aged Academic and Technical Leaders Reserve Talent Project (No. 202105AC160060)Yunnan Province High-level Talent Training Support Program “Youth Top Talent” Project (2020)。
文摘The development of cost-effective and high-efficiency catalysts for sustainable hydrogen production through electrocatalytic hydrogen evolution reaction(HER) is crucial yet remains challenging. In this work, we synthesized two types of bimetallic Pt Ni nanoparticles embedded in N-doped porous carbons derived from Ni-ABDC(5-aminoisophthalate) using both in-situ and ex-situ Pt inclusion methods. The in-situ Pt doping notably disrupted the effective growth of Ni-ABDC nanostrips owing to strong interactions between Pt and ABDC, resulting in an amorphous nanostructure. The optimized PtinNi-NC exhibited remarkable HER performance with a low overpotential of 29 mV at 10 mA/cm^(2), a Tafel slope of 47.4 mV/dec, and a current retention of 91.2% after 200 h in 1.0 mol/L KOH solution, surpassing the performance of Ni-NC, PtexNi-NC, and Pt/C. This research demonstrates the rational design and preparation of transition metal-based coordination polymer-derived metal-carbon nanomaterials with low Pt loading,emphasizing their considerable potential in energy conversion and storage technologies.
基金supported by National Natural Science Foundation of China(grant number 42101318)the National Key R&D Program of China(grant number 2018YFD1100101)。
文摘Ozone(O_(3))pollution has a profound impact on human health,vegetation development,and the ecological environment,making it a critical focus of global academic research.In recent years,O_(3)pollution in China has been on a steady rise,with ozone emerging as the sole conventional pollutant to consistently increase in concentration without any decline.This study conducted a quantitative analysis of O_(3)concentrations across 367 Chinese cities in 2019,examining spatial autocorrelation and local clustering of O_(3)levels,and investigated the diverse relationships between human activity factors and O_(3)concentration.The seasonal fluctuation of O_(3)exhibited the“M-type”pattern,with peak concentrations in winter and the lowest levels in summer.The center of O_(3)pollution migrated southeastward,with the area of highest concentration progressively shifting south along the eastern coast.Moreover,O_(3)concentration showed a strong positive correlation with population density,road freight volume,and industrial emissions,suggesting that human activities,vehicle emissions,and industrial operations are significant contributors to O_(3)production.The results provide comprehensive information on the characteristics,causes,and occurrence mechanism of O_(3)in Chinese cities that can be utilized by global government departments to formulate strategies to prevent and control O_(3)pollution.
基金supported by the National Key Research and Development Program of China,Grant Number:2021YFF0502900,2019YFC1604604National Natural Science Foundation of China,Grant Number:62075013,62027824.
文摘Tumor vaccine therapy offers significant advantages over conventional treatments,including reduced toxic side effects.However,it currently functions primarily as an adjuvant treatment modality in clinical oncology due to limitations in tumor antigen selection and delivery methods.Tumor vaccines often fail to elicit a sufficiently robust immune response against progressive tumors,thereby limiting their clinical efficacy.In this study,we developed a nanoparticle-based tumor vaccine,OVA@HA-PEI,utilizing ovalbumin(OVA)as the presenting antigen and hyaluronic acid(HA)and polyethyleneimine(PEI)as adjuvants and carriers.This formulation significantly enhanced the proliferation of immune cells and cytokines,such as CD3,CD8,interferon-,and tumor necrosis factor-,in vivo,effectively activating an immune response against B16–F10 tumors.In vivofluorescenceflow cytometry(IVFC)has already become an effective method for monitoring circulating tumor cells(CTCs)due to its direct,noninvasive,and long-term detection capabilities.Our study utilized a laboratory-constructed IVFC system to monitor the immune processes induced by the OVA@HA-PEI tumor vaccine and an anti-programmed death-1(PD-1)antibody.The results demonstrated that the combined treatment of OVA@HA-PEI and anti-PD-1 antibody significantly improved the survival time of mice compared to anti-PD-1 antibody treatment alone.Additionally,this combination therapy substantially reduced the number of CTCs in vivo,increased the clearance rate of CTCs by the immune system,and slowed tumor progression.Thesefindings greatly enhance the clinical application prospects of IVFC and tumor vaccines.
基金financially supported by the National Natural Science Foundation of China(No.42090053,No.41922054).
文摘Due to the joint development characteristic and macropore structure of loess,it is easy to cause structure collapse under earthquake or artificial vibration.The study on the loess disaster effect and its mechanism under earthquake action is insufficient due to its complexity.Hence,to study the deformation and mechanical properties more accurately,the dynamic characteristics of saturated remolded loess under cyclic dynamic load were tested using a GDS dynamic triaxial instrument in this paper.The test results show that strain and pore water pressure increase gradually at different rates with the development of vibration,and there is an obvious inflection point in the time-history curve of both.When the number of vibrations(N)exceeds this point,the strain increases rapidly,and pore water pressure tends to be stable.Under the action of large amplitude and low-frequency dynamic load,the strain and pore water pressure increase rapidly with fewer vibrations and the deviator stress(q)decreases rapidly,while the sample achieves damage faster with the increase of amplitude.During the application of a dynamic load,the effective stress(p)gradually decreases and its rate of change slows down.Finally,when the saturated remolded loess is subjected to a constant-amplitude dynamic load,the combination of large amplitude and low frequency leads to the failure of the sample in the shortest time.
基金supported by the Key R&D Program of Zhejiang(2024SSYS0022)the National Natural Science Foundation of China(31930028,91842301,32370608,32250710678,32200073,32250710678)+4 种基金Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2021R01012)“Pioneer”R&D programs of Zhejiang Province(2024C03005)STI2030-Major Projects(2021ZD0202502)the National Key Research and Development Program of China(2022YFC3400400,2018YFA0800503,2018YFA0107804)Beijing Municipal Science and Technology Commission(Z221100007022002).
文摘The applications of single-cell and spatial technologies in recent times have revolutionized the present understanding of cellular states and the cellular heterogeneity inherent in complex biological systems.These advancements offer unprecedented resolution in the examination of the functional genomics of individual cells and their spatial context within tissues.In this review,we have comprehensively discussed the historical development and recent progress in the field of single-cell and spatial genomics.We have reviewed the breakthroughs in single-cell multi-omics technologies,spatial genomics methods,and the computational strategies employed toward the analyses of singlecell atlas data.Furthermore,we have highlighted the advances made in constructing cellular atlases and their clinical applications,particularly in the context of disease.Finally,we have discussed the emerging trends,challenges,and opportunities in this rapidly evolving field.
基金This work was supported by grants from the National Key Research and Development Program of China(Grant No.2019YFC1604604)the Special Fund for Research on National Major Research Instruments of China(Grant No.62027824)+5 种基金the National Science Fund for Distinguished Young Scholars(Grant No.61425006)the SJTU Medicine Engineering Interdisciplinary Research Fund(Grant No.YG2017MS19)the Program of Shanghai Technology Research Leader(Grant No.17XD1402200)the Fundamental Research Funds for the Central Universities(Grant No.81661168014)the project of Innovative Research Team of High-level Local Universities in Shanghai,Talents Program of Shanghai Municipal Health Commission(Chen Liang,Grant No.2018BR06)the National Natural Science Foundation of China(Grant No.92068111,81973272,82073836,61975118,and 62075013).
文摘Photobiomodulation,by utilizing low-power light in the visible and near-infrared spectra to trigger biological responses in cells and tissues,has been considered as a possible therapeutic strategy for Alzheimer’s disease(AD),while its specific mechanisms have remained elusive.Here,we demonstrate that cognitive and memory impairment in an AD mouse model can be ameliorated by 1070-nm light via reducing cerebralβ-amyloid(Aβ)burden,the hallmark of AD.The glial cells,including microglia and astrocytes,play important roles in Aβclearance.Our results show that 1070-nm light pulsed at 10 Hz triggers microglia rather than astrocyte responses in AD mice.The 1070-nm lightinduced microglia responses with alteration in morphology and increased colocalization with Aβare sufficient to reduce Aβload in AD mice.Moreover,1070-nm light pulsed at 10 Hz can reduce perivascular microglia and promote angiogenesis to further enhance Aβclearance.Our study confirms the important roles of microglia and cerebral vessels in the use of 1070-nm light for the treatment of AD mice and provides a framework for developing a novel therapeutic approach for AD.
基金the National Key Research and Development Program of China(Grant No.2019YFC1604604)the National Science Fund for Distinguished Young Scholars(Grant No.61425006)+2 种基金the SJTU Medicine Engineering Interdisciplinary Research Fund(Grant No.YG2017MS19)the Program of Shanghai Technology Research Leader(Grant No.17XD1402200)the Fundamental Research Funds for the Central Universities,and the National Natural Science Foundation of China(Grant No.81661168014,61975118 and 62075013).
文摘Circulating tumor cells(CTCs)is an established biomarker of cancer metastasis.The circulation dynamics of CTCs are important for understanding the mechanisms underlying tumor cell dissemination.Although studies have revealed that the circadian rhythm may disrupt the growth of tumors,it is generally unclear whether the circadian rhythm controls the release of CTCs.In clinical examinations,the current in vitro methods for detecting CTCs in blood samples are based on a fundamental assumption that CTC counts in the peripheral blood do not change significantly over time,which is being challenged by recent studies.Since it is not practical to draw blood from patients repeatedly,a feasible strategy to investigate the circadian rhythm of CTCs is to monitor them by in vivo detection methods.Fluoresce nee in vivo flow cytometry(IVFC)is a powerful optical technique that is able to detect fluoresce nt circulating cells directly in living animals in a noninvasive manner over a long period of time.In this study,we applied fluorescence IVFC to monitor CTCs noninvasively in an orthotopic mouse model of human prostate cancer.We observed that CTCs exhibited stochastic bursts over cancer progression.The probability of the bursting activity was higher at early stages than at late stages.We longitudinally monitored CTCs over a 24-h period,and our results revealed striking daily oscillations in CTC counts that peaked at the onset of the night(active phase for rodents),suggesting that the release of CTCs might be regulated by the circadian rhythm.
文摘Dear Editor,We thank Niedre et al.for their correspondence regarding our recent paper1.They proposed a point that the temporal distribution of circulating tumor cells(CTCs)monitored by diffuse in vivo flow cytometry in a multiple myeloma mouse model in their previous work2 might be different from our results.Niedre et al.claimed that CTC detection statistics deviated from Poisson but did not found circadian variations in CTC numbers in a multiple myeloma mouse model.They also cite another literature by Juratli.et al.3,in which the authors reported that CTC numbers did not always correlate with tumor size during cancer progression.However,by establishing an orthotopic mouse model of prostate cancer and utilizing the technology in vivo flow cytometry(IVFC),we found CTCs exhibited bursting activity and daily oscillation in an orthotopic model of prostate cancer.
文摘Mathematical modelling performs a vital part in estimating and controlling the recent outbreak of coronavirus disease 2019(COVID-19).In this epidemic,most countries impose severe intervention measures to contain the spread of COVID-19.The policymakers are forced to make difficult decisions to leverage between health and economic development.How and when tomake clinical and public health decisions in an epidemic situation is a challenging question.The most appropriate solution is based on scientific evidence,which is mainly dependent on data and models.So one of the most critical problems during this crisis is whether we can develop reliable epidemiological models to forecast the evolution of the virus and estimate the effectiveness of various intervention measures and their impacts on the economy.There are numerous types of mathematical model for epidemiological diseases.In this paper,we present some critical reviews on mathematical models for the outbreak of COVID-19.Some elementary models are presented as an initial formulation for an epidemic.We give some basic concepts,notations,and foundation for epidemiological modelling.More related works are also introduced and evaluated by considering epidemiological features such as disease tendency,latent effects,susceptibility,basic reproduction numbers,asymptomatic infections,herd immunity,and impact of the interventions.
基金supported by the National Natural Science Foundation of China(21601137)the Basic Science and Technology Research Project of Wenzhou,Zhejiang Province(G20240038)+2 种基金the Special Basic Cooperative Research Programs of Yunnan Provincial Undergraduate Universities Association(202101BA070001-031,202101BA070001-042 and 202301BA070001-093)the Yunnan Province Young and Middle-aged Academic and Technical Leaders Reserve Talent Project(202105AC160060)the Yunnan Province High-level Talent Training Support Program“Youth Top Talent”Project(2020).
文摘Electrocatalytic water electrolysis is intrinsically limited by the slow kinetics of the oxygen evolution reaction(OER)at the anodic electrode.Building on our previous work,we utilized a porous metal-organic framework(CoOF-1)structurally characterized by rich adsorption sites for Ru(Ⅲ)ions.In this study,the incorporation of noble metal species into the CoOF-1-derived porous Co_(3)O_(4) matrix effectively improves electrocatalytic OER performance.
