The Yarlung Tsangpo River on the Tibetan Plateau provides a unique natural environment for studying fish evolution and ecology.However,the genomes and genetic diversity of plateau fish species have been rarely reporte...The Yarlung Tsangpo River on the Tibetan Plateau provides a unique natural environment for studying fish evolution and ecology.However,the genomes and genetic diversity of plateau fish species have been rarely reported.Schizopygopsis younghusbandi,a highly specialized Schizothoracine species and economically important fish inhabiting the Yarlung Tsangpo River,is threatened by overfishing and biological invasion.Herein,we generated a chromosome-level genome of S.younghusbandi and whole-genome resequencing data for 59 individuals from six locations of the river.The results showed that the divergence time between S.younghusbandi and other primitive Schizothoracine species was~4.2 Mya,coinciding with the major phase of the Neogene Tibetan uplift.The expanded gene families enriched in DNA integration and replication,ion binding and transport,energy storage,and metabolism likely contribute to the adaption of this species.The S.younghusbandi may have diverged from other highly specialized Schizothoracine species in the Zanda basin during the Pliocene epoch,which underwent major population reduction possibly due to the drastic climate change during the last glacial period.Population analysis indicated that the ancient population might have originated upstream before gradually adapting to evolve into the populations inhabiting the mid-stream and downstream regions of the Yarlung Tsangpo River.In conclusion,the chromosome-level genome and population diversity of S.younghusbandi provide valuable genetic resources for the evolution,ecology,and conservation studies of endemic fishes on the Tibetan Plateau.展开更多
Agrobacterium tumefaciens mediated transgenic system is an effective and convenient method for plant breeding.However,the method was almost conducted in uitro,which requires strict tissue culture conditions and it was...Agrobacterium tumefaciens mediated transgenic system is an effective and convenient method for plant breeding.However,the method was almost conducted in uitro,which requires strict tissue culture conditions and it was time consuming from tissue culture plantlets to field evaluation.Therefore,it is crucial to develop a tissue-culture-free field in uiuo transgenic technique.In the current study,we established a novel field in uiuo A.tumefaciens mediated genetic transformation system for Chinese jujube with a pink colormarker gene SUPER RUBY.展开更多
基金supported by the National Natural Science Foundation of China[grant numbers 32160864,32072980]the National Natural Science Foundation of China(NSFC)Joint Fund Priority Support Program[grant number U23A20249]+1 种基金the National Talent Research Grant for 2023[grant number 5330500953]the Special Fund for Youth Team of the Southwest University[grant number SWUXJPY202302].
文摘The Yarlung Tsangpo River on the Tibetan Plateau provides a unique natural environment for studying fish evolution and ecology.However,the genomes and genetic diversity of plateau fish species have been rarely reported.Schizopygopsis younghusbandi,a highly specialized Schizothoracine species and economically important fish inhabiting the Yarlung Tsangpo River,is threatened by overfishing and biological invasion.Herein,we generated a chromosome-level genome of S.younghusbandi and whole-genome resequencing data for 59 individuals from six locations of the river.The results showed that the divergence time between S.younghusbandi and other primitive Schizothoracine species was~4.2 Mya,coinciding with the major phase of the Neogene Tibetan uplift.The expanded gene families enriched in DNA integration and replication,ion binding and transport,energy storage,and metabolism likely contribute to the adaption of this species.The S.younghusbandi may have diverged from other highly specialized Schizothoracine species in the Zanda basin during the Pliocene epoch,which underwent major population reduction possibly due to the drastic climate change during the last glacial period.Population analysis indicated that the ancient population might have originated upstream before gradually adapting to evolve into the populations inhabiting the mid-stream and downstream regions of the Yarlung Tsangpo River.In conclusion,the chromosome-level genome and population diversity of S.younghusbandi provide valuable genetic resources for the evolution,ecology,and conservation studies of endemic fishes on the Tibetan Plateau.
基金funding from the National Key Research and Development Project(Grant No.2024YFD2200600)Hebei Provincial Key Research and Development Project(Grant No.21326304D)+1 种基金the Construction of Innovative Teams for the Modern Agricultural Industry System in Hebei Province,China(Grant Nos.HBCT2024190203,HBCT2024190201)Hebei Special Project for Technological Innovation in Modern Seed Industry(Grant No.23327501D)。
文摘Agrobacterium tumefaciens mediated transgenic system is an effective and convenient method for plant breeding.However,the method was almost conducted in uitro,which requires strict tissue culture conditions and it was time consuming from tissue culture plantlets to field evaluation.Therefore,it is crucial to develop a tissue-culture-free field in uiuo transgenic technique.In the current study,we established a novel field in uiuo A.tumefaciens mediated genetic transformation system for Chinese jujube with a pink colormarker gene SUPER RUBY.
