Remote coral reef systems,such as atolls and isolated reefs,play critical roles in supporting marine biodiversity but are often underrepresented in ecological assessments due to logistical and methodological constrain...Remote coral reef systems,such as atolls and isolated reefs,play critical roles in supporting marine biodiversity but are often underrepresented in ecological assessments due to logistical and methodological constraints.Environmental DNA(eDNA)metabarcoding provides a highly sensitive and non-invasive alternative to conventional surveys,particularly in in remote reef systems.However,its application for comparing biodiversity and community structure across geomorphologically distinct reef types remain limited.In this study,we applied eDNA metabarcoding to investigate fish,mollusc,and echinoderm communities across Yongle Atoll and surrounding isolated reefs in the Xisha Islands,South China Sea during May-June 2022.A total of 121 fish,94 mollusc,and 77 echinoderm species were detected.Fish communities at Yongle Atoll exhibited higherα-diversity and ecological heterogeneity,likely due to its complex spatial structure and greater resource availability.In contrast,molluscs and echinoderms in isolated reefs showed higher evenness and local diversity,possibly reflecting environmental stability and spatial isolation.Non-metric multidimensional scaling analysis revealed significant dissimilarities in fish and echinoderm assemblages between reef types,and indicator species analysis identified Thalassoma lunare and Culcita quadrangularis as representative taxa contributing to this differentiation.Canonical correspondence analysis further indicated that live coral cover was the primary environmental driver shaping fish communities,while chlorophyll a concentration significantly influenced invertebrate distribution.These patterns likely reflect the combined effects of niche-based environmental filtering and spatial dispersal constraints,indicating divergent community assembly processes between atoll and isolated reef systems.Our findings not only demonstrate the capacity of eDNA to resolve fine-scale biodiversity patterns in offshore coral systems but also provide baseline data to inform region-specific conservation,ecological zoning,and early warning in vulnerable marine ecosystems.This research underscores the growing relevance of molecular tools for biodiversity monitoring across tropical reefs worldwide.展开更多
Background and Objectives:To investigate the capacity of clinical nutrition services in secondary and tertiary hospitals in the Sichuan Province,China.Methods and Study Design:Convenience sampling was used.Equestionna...Background and Objectives:To investigate the capacity of clinical nutrition services in secondary and tertiary hospitals in the Sichuan Province,China.Methods and Study Design:Convenience sampling was used.Equestionnaires were distributed to all eligible medical institutions in Sichuan through the official network of provincial and municipal clinical nutrition quality control centers.The data obtained were sorted in Microsoft Excel and analyzed by SPSS.Results:A total of 519 questionnaires were returned,of which 455 were valid.Only 228 hospitals were accessible to clinical nutrition services,of which 127 hospitals had independently set up clinical nutrition departments(CNDs).The ratio of clinical nutritionists to beds was 1:214.During the last decade,the rate of constructing new CNDs was maintained at approximately 5 units/year.A total of 72.4%of hospitals managed their clinical nutrition units as part of their medical technology departments.The specialist number ratio of senior,associate,intermediate and junior is approximately 1:4:8:10.There were 5 common charges for clinical nutrition.Conclusions:The sample representation was limited,and the capacity of clinical nutrition services may have been overestimated.Secondary and tertiary hospitals in Sichuan are currently in the second high tide of department establishment,with a positive trend of departmental affiliation standardization and a basic formation of a talent echelon.展开更多
基金The National Key Research and Development Program of China under contract Nos 2022ZDYF3106303 and 2022YFD2401302the National Natural Science Foundation of China under contract Nos 32360917,42376114,and 42166004+1 种基金the Hainan Province Science and Technology Special Fund under Contract Nos ZDYF2023SHFZ101 and ZDYF2024SHFZ070the Hainan University Start-up Funding for Scientific Research under contract Nos KYQD(ZR)-21130 and KYQD[ZR]-22058.
文摘Remote coral reef systems,such as atolls and isolated reefs,play critical roles in supporting marine biodiversity but are often underrepresented in ecological assessments due to logistical and methodological constraints.Environmental DNA(eDNA)metabarcoding provides a highly sensitive and non-invasive alternative to conventional surveys,particularly in in remote reef systems.However,its application for comparing biodiversity and community structure across geomorphologically distinct reef types remain limited.In this study,we applied eDNA metabarcoding to investigate fish,mollusc,and echinoderm communities across Yongle Atoll and surrounding isolated reefs in the Xisha Islands,South China Sea during May-June 2022.A total of 121 fish,94 mollusc,and 77 echinoderm species were detected.Fish communities at Yongle Atoll exhibited higherα-diversity and ecological heterogeneity,likely due to its complex spatial structure and greater resource availability.In contrast,molluscs and echinoderms in isolated reefs showed higher evenness and local diversity,possibly reflecting environmental stability and spatial isolation.Non-metric multidimensional scaling analysis revealed significant dissimilarities in fish and echinoderm assemblages between reef types,and indicator species analysis identified Thalassoma lunare and Culcita quadrangularis as representative taxa contributing to this differentiation.Canonical correspondence analysis further indicated that live coral cover was the primary environmental driver shaping fish communities,while chlorophyll a concentration significantly influenced invertebrate distribution.These patterns likely reflect the combined effects of niche-based environmental filtering and spatial dispersal constraints,indicating divergent community assembly processes between atoll and isolated reef systems.Our findings not only demonstrate the capacity of eDNA to resolve fine-scale biodiversity patterns in offshore coral systems but also provide baseline data to inform region-specific conservation,ecological zoning,and early warning in vulnerable marine ecosystems.This research underscores the growing relevance of molecular tools for biodiversity monitoring across tropical reefs worldwide.
基金supported by the Operating expenses of quality control center from Health Commission of Sichuan Province(TJCZ2016003)Key R&D Projects of Sichuan Science and Technology Bureau,the Construction and Transformation of Comprehensive Evaluation Index system for rational use of Food for Special Medical Purposes in Medical institutions(2021YFS0177).
文摘Background and Objectives:To investigate the capacity of clinical nutrition services in secondary and tertiary hospitals in the Sichuan Province,China.Methods and Study Design:Convenience sampling was used.Equestionnaires were distributed to all eligible medical institutions in Sichuan through the official network of provincial and municipal clinical nutrition quality control centers.The data obtained were sorted in Microsoft Excel and analyzed by SPSS.Results:A total of 519 questionnaires were returned,of which 455 were valid.Only 228 hospitals were accessible to clinical nutrition services,of which 127 hospitals had independently set up clinical nutrition departments(CNDs).The ratio of clinical nutritionists to beds was 1:214.During the last decade,the rate of constructing new CNDs was maintained at approximately 5 units/year.A total of 72.4%of hospitals managed their clinical nutrition units as part of their medical technology departments.The specialist number ratio of senior,associate,intermediate and junior is approximately 1:4:8:10.There were 5 common charges for clinical nutrition.Conclusions:The sample representation was limited,and the capacity of clinical nutrition services may have been overestimated.Secondary and tertiary hospitals in Sichuan are currently in the second high tide of department establishment,with a positive trend of departmental affiliation standardization and a basic formation of a talent echelon.
