Lung cancer is one of the greatest threats to human health. It is a very effective way to detect lung cancer by pathological pictures of lung cancer cells. Therefore, improving the accuracy and stability of diagnosis ...Lung cancer is one of the greatest threats to human health. It is a very effective way to detect lung cancer by pathological pictures of lung cancer cells. Therefore, improving the accuracy and stability of diagnosis is very important. In this study, we develop an automatic detection scheme for lung cancer cells based on convolutional neural networks and Swin Transformer. Microscopic images of patients’ lung cells are first segmented using a Mask R-CNN-based network, resulting in a separate image for each cell. Part of the background information is preserved by Gaussian blurring of surrounding cells, while the target cells are highlighted. The classification model based on Swin Transformer not only reduces the computation but also achieves better results than the classical CNN model, ResNet50. The final results show that the accuracy of the method proposed in this paper reaches 96.16%. Therefore, this method is helpful for the detection and classification of lung cancer cells.展开更多
The study aimed to investigate and compare the contents of carboxymethyllysine(CML)in two kinds of broilers during postmortem ageing and storage.The contents of CML in raw and boiled(100℃,30 min)broiler briskets and ...The study aimed to investigate and compare the contents of carboxymethyllysine(CML)in two kinds of broilers during postmortem ageing and storage.The contents of CML in raw and boiled(100℃,30 min)broiler briskets and legs which were from white feather broilers(n=8)and yellow feather broilers(n=8)with ageing and storage at 4℃for 0-168 h were determined.Postmortem ageing and storage had a significant(P<0.05)effect on the color and A;in both boiled broilers meat.In addition,with the ageing and storage time increasing,CML content in raw white feather broiler brisket meat had no significant(P>0.05)change,while that in boiled brisket meat significantly(P<0.05)increased during 0-6 h,then decreased during 6-24 h,finally increased again.CML content in leg meat increased significantly(P<0.05)with the ageing and storage time prolonging.But postmortem ageing and storage had no significant(P>0.05)effect on the CML contents in raw/boiled yellow feather broilers.Meanwhile,CML contents in white feather broilers were much higher than that in yellow feather broilers.Thus,white feather broilers can be selected as the research object to study the mechanism of ageing and storage on CML content in the postmortem broiler in the future.展开更多
Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form ...Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47 × 10^5±0.11× 10^5 U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to S.0 （optimum pH 3.5）. The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil （used in planting trees） and rice soil （originating as red soil）. The available phosphorus content was increased by 230% after inoculating 20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.展开更多
Fiber optic sensors have been gradually used in aerospace,petrochemical,electronic power,civil engineering,and biomedical fields because of their many advantages such as the anti-electromagnetic interference,corrosion...Fiber optic sensors have been gradually used in aerospace,petrochemical,electronic power,civil engineering,and biomedical fields because of their many advantages such as the anti-electromagnetic interference,corrosion resistance,light weight,small size,high accuracy,and easy reuse.In recent years,sensing and demodulation technologies based on microwave photonics have attracted widespread attention.Optical fiber sensing combined with microwave photonics has higher sensitivity and flexibility,which is important for the demodulation of interferometric signals.This article introduces and analyzes the principle,structure,and performance of the demodulation technology of fiber optic interferometric signals based on microwave photonics from the perspective of system structures,such as filters,oscillators,and interferometers,and discusses the future research and development directions.展开更多
To understand the mechanism governing the postharvest senescence of Zizania latifolia(Z.latifolia),and the regulatory mechanism induced by 1-methylcyclopropene(1-MCP)during storage at 25℃,physiobiochemical and conjoi...To understand the mechanism governing the postharvest senescence of Zizania latifolia(Z.latifolia),and the regulatory mechanism induced by 1-methylcyclopropene(1-MCP)during storage at 25℃,physiobiochemical and conjoint analyses of the transcriptome and metabolome were performed.The results indicated that 1-MCP treatment engendered changes in the expression of genes and metabolites during the postharvest storage of Z.latifolia.The 1-MCP treatment maintained a good visual appearance,preserved the cell structure and membrane integrity of Z.latifolia by keeping the expression of membrane-related lipolytic enzymes(and related genes)low and the amount of phosphatidylethanolamine high.Compared to the control group,1-MCP treatment enhanced the activities of antioxidant enzymes,resulting in a decrease of reactive oxygen species and malondialdehyde contents,and thus inhibition of oxidative damage and loss of membrane integrity.In addition,1-MCP treatment retarded the senescence of Z.latifolia by downregulating the expression of ethylene biosynthesis-related genes and promoting upregulation of brassinosteroid insensitive 1 kinase inhibitor 1,calmodulin,glutathione reductase,jasmonate amino acid synthase,and mitogen-activated protein kinase-related genes.Moreover,1-MCP retarded Z.latifolia senescence by inducing the activity of adenosine triphosphate(ATP)biosynthesis-related genes and metabolites.Our findings should facilitate future research on the postharvest storage of Z.latifolia,and could help delay senescence and prolong the storage time for commercial applications.展开更多
文摘Lung cancer is one of the greatest threats to human health. It is a very effective way to detect lung cancer by pathological pictures of lung cancer cells. Therefore, improving the accuracy and stability of diagnosis is very important. In this study, we develop an automatic detection scheme for lung cancer cells based on convolutional neural networks and Swin Transformer. Microscopic images of patients’ lung cells are first segmented using a Mask R-CNN-based network, resulting in a separate image for each cell. Part of the background information is preserved by Gaussian blurring of surrounding cells, while the target cells are highlighted. The classification model based on Swin Transformer not only reduces the computation but also achieves better results than the classical CNN model, ResNet50. The final results show that the accuracy of the method proposed in this paper reaches 96.16%. Therefore, this method is helpful for the detection and classification of lung cancer cells.
基金supported by the China Agriculture Research System(CARS-41-Z)。
文摘The study aimed to investigate and compare the contents of carboxymethyllysine(CML)in two kinds of broilers during postmortem ageing and storage.The contents of CML in raw and boiled(100℃,30 min)broiler briskets and legs which were from white feather broilers(n=8)and yellow feather broilers(n=8)with ageing and storage at 4℃for 0-168 h were determined.Postmortem ageing and storage had a significant(P<0.05)effect on the color and A;in both boiled broilers meat.In addition,with the ageing and storage time increasing,CML content in raw white feather broiler brisket meat had no significant(P>0.05)change,while that in boiled brisket meat significantly(P<0.05)increased during 0-6 h,then decreased during 6-24 h,finally increased again.CML content in leg meat increased significantly(P<0.05)with the ageing and storage time prolonging.But postmortem ageing and storage had no significant(P>0.05)effect on the CML contents in raw/boiled yellow feather broilers.Meanwhile,CML contents in white feather broilers were much higher than that in yellow feather broilers.Thus,white feather broilers can be selected as the research object to study the mechanism of ageing and storage on CML content in the postmortem broiler in the future.
基金supported by a grant from the Special Project for Forest Public Benefit (No. 200904055)Project for Advantage Life Science of Jiangshu Province and Open Project of Jiangsu Key Laboratory for Biodiversity and Biotechnology (No. 164070302115)
文摘Orthophosphate is an essential but limiting macronutrient for plant growth. About 67% cropland in China lacks sufficient phosphorus, especially that with red soil. Extensive soil phosphorus reserves exist in the form of organic phosphorus, which is unavailable for root uptake unless hydrolyzed by secretory acid phosphatases. Thus, many microorganisms with the ability to produce phosphatase have been exploited. In this work, the activity of an extracellular acid phosphatase and yeast biomass from Candida mycoderma was measured under different culture conditions, such as pH, temperature, and carbon source. A maximal phosphatase activity of 8.47 × 10^5±0.11× 10^5 U/g was achieved by C. Mycoderma in 36 hr under the optimal conditions. The extracellular acid phosphatase has high activity over a wide pH tolerance range from 2.5 to S.0 （optimum pH 3.5）. The effects of different phosphorus compounds on the acid phosphatase production were also studied. The presence of phytin, lecithin or calcium phosphate reduced the phosphatase activity and biomass yield significantly. In addition, the pH of the culture medium was reduced significantly by lecithin. The efficiency of the strain in releasing orthophosphate from organic phosphorus was studied in red soil （used in planting trees） and rice soil （originating as red soil）. The available phosphorus content was increased by 230% after inoculating 20 days in rice soil and decreased by 50% after inoculating 10 days in red soil. This work indicates that the yeast strain C. mycoderma has potential application for enhancing phosphorus utilization in plants that grow in rice soil.
基金supported in part by the National Natural Science Foundation of China(Grant Nos.52175530,51675068,51975077,and 61875023).
文摘Fiber optic sensors have been gradually used in aerospace,petrochemical,electronic power,civil engineering,and biomedical fields because of their many advantages such as the anti-electromagnetic interference,corrosion resistance,light weight,small size,high accuracy,and easy reuse.In recent years,sensing and demodulation technologies based on microwave photonics have attracted widespread attention.Optical fiber sensing combined with microwave photonics has higher sensitivity and flexibility,which is important for the demodulation of interferometric signals.This article introduces and analyzes the principle,structure,and performance of the demodulation technology of fiber optic interferometric signals based on microwave photonics from the perspective of system structures,such as filters,oscillators,and interferometers,and discusses the future research and development directions.
基金the Qingchuang Science and Technology Support Program of Shandong Provincial College(Lujiaorenzi[2019]No.0.6)the Research Start-up Funding from Nanjing Normal University(184080H202B117)the Doctoral Fund(XY 19BS18)of Heze University(provided to Huibo Song),China.
文摘To understand the mechanism governing the postharvest senescence of Zizania latifolia(Z.latifolia),and the regulatory mechanism induced by 1-methylcyclopropene(1-MCP)during storage at 25℃,physiobiochemical and conjoint analyses of the transcriptome and metabolome were performed.The results indicated that 1-MCP treatment engendered changes in the expression of genes and metabolites during the postharvest storage of Z.latifolia.The 1-MCP treatment maintained a good visual appearance,preserved the cell structure and membrane integrity of Z.latifolia by keeping the expression of membrane-related lipolytic enzymes(and related genes)low and the amount of phosphatidylethanolamine high.Compared to the control group,1-MCP treatment enhanced the activities of antioxidant enzymes,resulting in a decrease of reactive oxygen species and malondialdehyde contents,and thus inhibition of oxidative damage and loss of membrane integrity.In addition,1-MCP treatment retarded the senescence of Z.latifolia by downregulating the expression of ethylene biosynthesis-related genes and promoting upregulation of brassinosteroid insensitive 1 kinase inhibitor 1,calmodulin,glutathione reductase,jasmonate amino acid synthase,and mitogen-activated protein kinase-related genes.Moreover,1-MCP retarded Z.latifolia senescence by inducing the activity of adenosine triphosphate(ATP)biosynthesis-related genes and metabolites.Our findings should facilitate future research on the postharvest storage of Z.latifolia,and could help delay senescence and prolong the storage time for commercial applications.
