The upregulation of 4-hydroxyphenylpyruvate dioxygenase(HPPD)can protect plants from adverse abiotic stress.Therefore,studying the changes of HPPD under abiotic stress is extremely valuable.In this study,we employed a...The upregulation of 4-hydroxyphenylpyruvate dioxygenase(HPPD)can protect plants from adverse abiotic stress.Therefore,studying the changes of HPPD under abiotic stress is extremely valuable.In this study,we employed a rational molecular design strategy to prepare an HPPD-responsive fluorescent probe consisting of a pyrene fluorophore,a linker and a benquitrione skeleton recognition moiety that functions via an aggregation–disaggregation sensing mechanism.As predicted,this probe exhibited an obvious turn-on fluorescence response towards HPPD.In addition,in vivo imaging demonstrated the excellent capability of this probe to track HPPD in Arabidopsis thaliana,and the dynamic changes in HPPD were monitored under different stress degrees of high temperature and Cadmium(II)ion(Cd^(2+))stress.This work provides an efficient design strategy for acquiring a noninvasive HPPD fluorescence probe,which could evaluate the degree of stress and be further employed for exploring the stress resistance mechanism in plants.展开更多
Viruses are ubiquitous in human life. Some viruses can be used as vectors of genetic engineering and specific pesticides. Other viruses trigger a variety of diseases in humans, animals and plants, resulting in high in...Viruses are ubiquitous in human life. Some viruses can be used as vectors of genetic engineering and specific pesticides. Other viruses trigger a variety of diseases in humans, animals and plants, resulting in high infection rates and mortality. Therefore, convenient, accurate and rapid detection of viruses is of great significance for the diagnosis and treatment of subsequent diseases. In contrast to traditional methods of detection, which rely on time-consuming and complex techniques such as polymerase chain reaction (PCR), fluorescent probes and imaging methods generate real-time results, with high specificity, and have been widely used in viral detection. In this review, the application of viral fluorescent probes in analyzing the molecular structure, detection and biological imaging is discussed. In particular, we categorized the probes based on their specificity for human and plant viruses, reviewing the latest findings and analyzing their limitations. The potential of fluorescent molecular probes in the treatment of viral disease and environmental analysis, and their possible combinations with protein and immune technology are discussed.展开更多
Due to their excellent fluorescence properties and biological function,cyanine dyes have been widely applied in biological imaging.Heptamethine cyanine(Cy7)dyes,as a type of classic near-infrared(NIR)fluorescent dyes,...Due to their excellent fluorescence properties and biological function,cyanine dyes have been widely applied in biological imaging.Heptamethine cyanine(Cy7)dyes,as a type of classic near-infrared(NIR)fluorescent dyes,are considered as one of the effective fluorescent tools in the living organisms due to their good biocompatibility and very low background interference.Bioorthogonal reactions performed in living cells and tissues have developed by leaps and bounds in recent years.The NIR fluorescent labeling technique involving cyanine has attracted widespread attention.This review summarizes their recent application in the field of bioorthogonal imaging,mainly concluding Cy7-type dyes,labeling strategy,bioimaging application,etc.We expect this work can provide some helps for the studies of NIR bioorthogonal reaction in vivo.展开更多
Heptamethine cyanine dyes,typified by indocyanine green,have been extensively employed as bioimaging indicators and theranostic agents.Significant efforts have been made to develop functional heptamethine cyanine dyes...Heptamethine cyanine dyes,typified by indocyanine green,have been extensively employed as bioimaging indicators and theranostic agents.Significant efforts have been made to develop functional heptamethine cyanine dyes with outstanding bioimaging and theranostic utilities.In this work,we rationally designed and successfully developed a novel indolium-like heptamethine cyanine dye by installing indolium-derived polycyclic aromatic hydrocarbons on the terminal ends of a conjugated polyene backbone.This dye showed excellent photostability and showed bright fluorescent emission in the second near-infrared(NIR-Ⅱ)window with a peak at approximately 1120 nm.Such long wavelength emission prompted a superior bioimaging resolution in vivo.In particular,this NIR-Ⅱ dye had the remarkable capability of marking the blood vessels of the hindlimbs,abdomens,and brains of mice.More significantly,this dye involved a typical indolium-like heptamethine skeleton and exhibited two strong absorption bands in the 700–1300 nm NIR range,which endowed it with an intrinsic tumor-targeting capability and a high photothermal conversion efficiency(up to 68.2%),serving for the photothermal therapy of tumors under the guidance of NIR-Ⅱ fluorescence imaging.This work provides an efficient design strategy for achieving indolium-like heptamethine cyanine dyes with further NIR-Ⅱ emission.展开更多
Cell wall is a basic component of bacteria that promotes bacteria to adapt the complicated environment as well as play an essential role in antimicrobial resistance.The structure of bacterial cell wall is remarkably r...Cell wall is a basic component of bacteria that promotes bacteria to adapt the complicated environment as well as play an essential role in antimicrobial resistance.The structure of bacterial cell wall is remarkably rich,and some complex components,such as peptidoglycan,lipopolysaccharide,and peptidoglycan-arabinogalactan et al.,can only be found in bacteria.Furthermore,the biosynthesis and transfer of these glycolipids are indispensable for bacteria during cell elongation.And the process of biosynthesis and transfer are generally associated with metabolism and sophisticated enzyme mechanisms.However,how the metabolic process takes place,what role enzymes play in this process and how they function have been major concerns for scientists in this field.Numerous significant progresses on fluorescent probes and biological imaging bring opportunity for the studying of metabolism and enzyme mechanisms recently due to its non-invasive and high sensitivity.In this review,we focused on metabolic fluorescent probes for the detection of glycolipids in bacterial cell wall and highlighted the involving mechanisms and biological application.展开更多
基金supported by the National Key R&D Program of China(No.2021YFD1700103)National Natural Science Foundation of China(21837001,21676113,21772054,22007035)+1 种基金the 111 Project B17019,the Scholar support program of CCNU(0900–31101090002)Hubei Province Natural Science Foundation(No.2020CFB487).
文摘The upregulation of 4-hydroxyphenylpyruvate dioxygenase(HPPD)can protect plants from adverse abiotic stress.Therefore,studying the changes of HPPD under abiotic stress is extremely valuable.In this study,we employed a rational molecular design strategy to prepare an HPPD-responsive fluorescent probe consisting of a pyrene fluorophore,a linker and a benquitrione skeleton recognition moiety that functions via an aggregation–disaggregation sensing mechanism.As predicted,this probe exhibited an obvious turn-on fluorescence response towards HPPD.In addition,in vivo imaging demonstrated the excellent capability of this probe to track HPPD in Arabidopsis thaliana,and the dynamic changes in HPPD were monitored under different stress degrees of high temperature and Cadmium(II)ion(Cd^(2+))stress.This work provides an efficient design strategy for acquiring a noninvasive HPPD fluorescence probe,which could evaluate the degree of stress and be further employed for exploring the stress resistance mechanism in plants.
基金National Natural Science Foundation of China(No.22274061)the 111 Project B17019.Supported by the Fundamental Research Funds for the Central Universities(No.CCNU22QN007)Opening fund of Hubei Key Laboratory of Bioinorganic Chemistry&Materia Medica(No.BCMM202101).
文摘Viruses are ubiquitous in human life. Some viruses can be used as vectors of genetic engineering and specific pesticides. Other viruses trigger a variety of diseases in humans, animals and plants, resulting in high infection rates and mortality. Therefore, convenient, accurate and rapid detection of viruses is of great significance for the diagnosis and treatment of subsequent diseases. In contrast to traditional methods of detection, which rely on time-consuming and complex techniques such as polymerase chain reaction (PCR), fluorescent probes and imaging methods generate real-time results, with high specificity, and have been widely used in viral detection. In this review, the application of viral fluorescent probes in analyzing the molecular structure, detection and biological imaging is discussed. In particular, we categorized the probes based on their specificity for human and plant viruses, reviewing the latest findings and analyzing their limitations. The potential of fluorescent molecular probes in the treatment of viral disease and environmental analysis, and their possible combinations with protein and immune technology are discussed.
基金supported by the National Key R&D Program of China(No.2022YFA1207400)National Natural Science Foundation of China(No.22274061)+1 种基金the 111 Project(No.B17019)Fundamental Research Funds for the Central Universities(No.CCNU22QN007).
文摘Due to their excellent fluorescence properties and biological function,cyanine dyes have been widely applied in biological imaging.Heptamethine cyanine(Cy7)dyes,as a type of classic near-infrared(NIR)fluorescent dyes,are considered as one of the effective fluorescent tools in the living organisms due to their good biocompatibility and very low background interference.Bioorthogonal reactions performed in living cells and tissues have developed by leaps and bounds in recent years.The NIR fluorescent labeling technique involving cyanine has attracted widespread attention.This review summarizes their recent application in the field of bioorthogonal imaging,mainly concluding Cy7-type dyes,labeling strategy,bioimaging application,etc.We expect this work can provide some helps for the studies of NIR bioorthogonal reaction in vivo.
基金supported by the National Basic Research Foundation of China(no.2017YFA0505202)financial support from the National Natural Science Foundation of China(nos.21676113 and 21772054)+6 种基金the Distinguished Young Scholar of Hubei Province(no.2018CFA079)the 111 Project B17019the Scholar Support Program of CCNU(no.0900-31101090002)financial support from A*STAR under its Advanced Manufacturing and Engineering Program(no.A2083c0051)the Ministry of Education,Singapore(no.MOE-MOET2EP10120-0007)supported by grants from the 619 National Creative Research Initiative programs of the 620 National Research Foundation of Koreafunded by 621 the Korean government(no.2021R1A6A1A10039823).
文摘Heptamethine cyanine dyes,typified by indocyanine green,have been extensively employed as bioimaging indicators and theranostic agents.Significant efforts have been made to develop functional heptamethine cyanine dyes with outstanding bioimaging and theranostic utilities.In this work,we rationally designed and successfully developed a novel indolium-like heptamethine cyanine dye by installing indolium-derived polycyclic aromatic hydrocarbons on the terminal ends of a conjugated polyene backbone.This dye showed excellent photostability and showed bright fluorescent emission in the second near-infrared(NIR-Ⅱ)window with a peak at approximately 1120 nm.Such long wavelength emission prompted a superior bioimaging resolution in vivo.In particular,this NIR-Ⅱ dye had the remarkable capability of marking the blood vessels of the hindlimbs,abdomens,and brains of mice.More significantly,this dye involved a typical indolium-like heptamethine skeleton and exhibited two strong absorption bands in the 700–1300 nm NIR range,which endowed it with an intrinsic tumor-targeting capability and a high photothermal conversion efficiency(up to 68.2%),serving for the photothermal therapy of tumors under the guidance of NIR-Ⅱ fluorescence imaging.This work provides an efficient design strategy for achieving indolium-like heptamethine cyanine dyes with further NIR-Ⅱ emission.
基金financial support from the National Natural Science Foundation of China(21676113,21772054)the 111 Project B17019,Scholar Support Program of CCNU(0900–31101090002)+4 种基金Excellent Doctorial Dissertation Cultivation Grant of CCNU from the colleges’basic research and operation of MOE(2019YBZZ029)funded by the Research Fund Program of Guangdong Key Laboratory of Radioactive and Rare Resource Utilization(2018B030322009)supported by Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules(KLSAOFM2012)Hubei University,Wuhan 430062,P.R.China,Opening fund of Hubei Key Laboratory of Bioinorganic Chemistry&Materia Medica(No.BCMM202101)supported by State Key Laboratory of Chemical Oncogenomics(Shenzhen)
文摘Cell wall is a basic component of bacteria that promotes bacteria to adapt the complicated environment as well as play an essential role in antimicrobial resistance.The structure of bacterial cell wall is remarkably rich,and some complex components,such as peptidoglycan,lipopolysaccharide,and peptidoglycan-arabinogalactan et al.,can only be found in bacteria.Furthermore,the biosynthesis and transfer of these glycolipids are indispensable for bacteria during cell elongation.And the process of biosynthesis and transfer are generally associated with metabolism and sophisticated enzyme mechanisms.However,how the metabolic process takes place,what role enzymes play in this process and how they function have been major concerns for scientists in this field.Numerous significant progresses on fluorescent probes and biological imaging bring opportunity for the studying of metabolism and enzyme mechanisms recently due to its non-invasive and high sensitivity.In this review,we focused on metabolic fluorescent probes for the detection of glycolipids in bacterial cell wall and highlighted the involving mechanisms and biological application.
