To accomplish on-site separation, preconcentration and cold storage of highly volatile organic compounds(VOCs) from water samples as well as their rapid transportation to laboratory, a high-throughput miniaturized pur...To accomplish on-site separation, preconcentration and cold storage of highly volatile organic compounds(VOCs) from water samples as well as their rapid transportation to laboratory, a high-throughput miniaturized purge-and-trap(μP&T) device integrating semiconductor refrigeration storage was developed in this work. Water samples were poured into the purge vessels and purged with purified air generated by an air pump. The VOCs in water samples were then separated and preconcentrated with sorbent tubes. After their complete separation and preconcentration, the tubes were subsequently preserved in the semiconductor refrigeration unit of the μP&T device. Notably, the high integration, small size, light weight, and low power consumption of the device makes it easy to be hand-carried to the field and transport by drone from remote locations, significantly enhancing the flexibility of field sampling. The performances of the device were evaluated by comparing analytical figures of merit for the detection of four cyclic volatile methylsiloxanes(cVMSs) in water. Compared to conventional collection and preservation methods, our proposed device preserved the VOCs more consistently in the sorbent tubes, with less than 5% loss of all analytes, and maintained stability for at least 20 days at 4℃. As a proof-of-concept,10 municipal wastewater samples were pretreated using this device with recoveries ranging from 82.5% to 99.9% for the target VOCs.展开更多
MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,t...MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato.展开更多
Co-exposure to heavy metal and antibiotic pollution might result in complexation and synergistic interactions,affecting rice growth and further exacerbating pollutant enrichment.Therefore,our study sought to clarify t...Co-exposure to heavy metal and antibiotic pollution might result in complexation and synergistic interactions,affecting rice growth and further exacerbating pollutant enrichment.Therefore,our study sought to clarify the influence of different Tetracycline (TC) and Cadmium(Cd) concentration ratios (both alone and combined) on rice growth,pollutant accumulation,and transportation during the tillering stage in hydroponic system.Surprisingly,our findings indicated that the interaction between TC and Cd could alleviate the toxic effects of TC/Cd on aerial rice structures and decrease pollutant burdens during root elongation.In contrast,TC and Cd synergistically promoted the accumulation of TC/Cd in rice roots.However,their interaction increased the accumulation of TC in roots while decreasing the accumulation of Cd when the toxicant doses increased.The strong affinity of rice to Cd promoted its upward transport from the roots,whereas the toxic effects of TC reduced TC transport.Therefore,the combined toxicity of the two pollutants inhibited their upward transport.Additionally,a low concentration of TC promoted the accumulation of Cd in rice mainly in the root tip.Furthermore,a certain dose of TC promoted the upward migration of Cd from the root tip.Laser ablation-inductively coupled plasma mass spectrometry demonstrated that Cd mainly accumulated in the epidermis and stele of the root,whereas Fe mainly accumulated in the epidermis,which inhibited the absorption and accumulation of Cd by the rice roots through the generation of a Fe plaque.Our findings thus provide insights into the effects of TC and Cd co-exposure on rice growth.展开更多
Water-soluble triplet sensitizer with permethyl-β-cyclodextrin(PMCD)grafting on a Schiff-base Pt(II)complex(Pt-2),in which PMCD unit serves as a host for binding the acceptors and the Schiff-base Pt(II)complex serves...Water-soluble triplet sensitizer with permethyl-β-cyclodextrin(PMCD)grafting on a Schiff-base Pt(II)complex(Pt-2),in which PMCD unit serves as a host for binding the acceptors and the Schiff-base Pt(II)complex serves as a triplet sensitizer,was synthesized to investigate the effect of supramolecular complexation and assembly on the triplet-triplet annihilation upconversion emission in water.9,10-Diphenylanthracence(DPA)carboxylate(A-1)and its dimer(A-2)in which two DPA carboxylate were covalently linked with an alkyl chain were synthesized as triplet acceptors which also play a role of vip molecules for PMCD.A-1 and A-2 showed high affinity with PMCD,and A-2 can readily aggregate in water and form micron sized assemblies due to the hydrophobic effect andπ-πstacking of anthracene core in A-2.The efficiency of TTA-UC was demonstrated to be enhanced by a synergistic effect of host-vip complexation of Pt-2 with A-2 and the self-aggregation of the acceptor A-2,which facilitated the energy transfer and energy fusion among donor and acceptor.展开更多
Blood deficiency syndrome(BDS)refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine.Danggui Wuji granules(DWG)was developed from a formula containing Angelicae Sinen...Blood deficiency syndrome(BDS)refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine.Danggui Wuji granules(DWG)was developed from a formula containing Angelicae Sinensis Radix and Musculus et Os Galli Domestici.Herein,we investigated the mechanism of DWG in treating BDS by modulating gut microbiota.We found that DWG protected mice from BDS by elevating the levels of red blood cell count,hemoglobin,and hematocrit in peripheral blood and increasing the erythrocyte membrane Na+-K+-ATPase activity.Danggui Wuji granules changed the composition and metabolites of colonic flora.Notably,Lactobacillus,Muribaculaceae,and Alistipes were the main genera showing changes after DWG treatment.Our findings revealed that DWG presented a positive therapeutic effect on BDS in mice by regulating the gut microbiota and metabolites.The protective mechanism of DWG was associated with pathways such as metabolic pathways,biosynthesis of secondary metabolites,ABC transporters,ribosome,thyroid hormone synthesis,lysine degradation,galactose metabolism,tyrosine metabolism,etc.展开更多
基金the National Natural Science Foundation of China (No. 22306146)the PhD Scientific Research Startup Foundation of Xihua University (No. RX2200002003) for their financial support。
文摘To accomplish on-site separation, preconcentration and cold storage of highly volatile organic compounds(VOCs) from water samples as well as their rapid transportation to laboratory, a high-throughput miniaturized purge-and-trap(μP&T) device integrating semiconductor refrigeration storage was developed in this work. Water samples were poured into the purge vessels and purged with purified air generated by an air pump. The VOCs in water samples were then separated and preconcentrated with sorbent tubes. After their complete separation and preconcentration, the tubes were subsequently preserved in the semiconductor refrigeration unit of the μP&T device. Notably, the high integration, small size, light weight, and low power consumption of the device makes it easy to be hand-carried to the field and transport by drone from remote locations, significantly enhancing the flexibility of field sampling. The performances of the device were evaluated by comparing analytical figures of merit for the detection of four cyclic volatile methylsiloxanes(cVMSs) in water. Compared to conventional collection and preservation methods, our proposed device preserved the VOCs more consistently in the sorbent tubes, with less than 5% loss of all analytes, and maintained stability for at least 20 days at 4℃. As a proof-of-concept,10 municipal wastewater samples were pretreated using this device with recoveries ranging from 82.5% to 99.9% for the target VOCs.
基金supported by the Gansu Science and Technology Major Project(Grant No.22ZD6NA009)the Key Program of Natural Science Foundation of Gansu Province(Grant No.22JR5RA832)+1 种基金the Gansu Science and Technology Major Project(Grant No.23ZDNA006)National Natural Science Foundation of China(Grant No.31860399).
文摘MicroRNAs(miRNAs) are a class of small endogenous non-coding RNAs that direct post-transcriptional gene silencing.In plants,numerous miRNAs have been demonstrated to be regulated under drought-induced stress.However,the role of miRNAs in drought regulation remains unclear in potato.In this work,the function of stu-miR159a was investigated in responding to drought stress in potato.Upon examination,StGAMyb-like1 was identified as the target gene for stu-miR159a.Overexpression of stu-miR159a(stu-miR159a OE plants) increased sensitivity to drought,interference with stu-miR159a activity by target mimics(stu-miR159a ST plants) resulted in drought resistance.During drought treatment,the target gene StGAMyb-like1 showed increased activation in stu-miR159a ST plants compared to non-transgenic plants.In contrast,drought stress induced weaker activation of the target gene in stu-miR159a OE plants.In stu-miR159a ST plants,the expression of critical genes in the anthocyanin biosynthesis pathway(StF3'5'H,StF3'H and StCHS2)was increased by decreasing stu-miR159a activity and simultaneously increasing that of StGAMyb-like1.Meanwhile,with drought treatment,stu-miR159a ST plants exhibited higher anthocyanin accumulation than non-transgenic ones,indicating enhanced antioxidant capacity and improved drought tolerance.The above data support that stu-miR159a is a negative regulator of drought stress and provide new insights into the stu-miR159a-mediated regulation of the anthocyanin biosynthesis pathway in controlling drought tolerance in potato.
基金supported by the National Natural Science Foundation of China (No.41773107)the Fundamental and Applied Basic Research Foundation of Guangdong Province,China (No.2020A1515011560)the Science and Technology Planning Project of Guangzhou,Guangdong Province,China(No.202002030297)。
文摘Co-exposure to heavy metal and antibiotic pollution might result in complexation and synergistic interactions,affecting rice growth and further exacerbating pollutant enrichment.Therefore,our study sought to clarify the influence of different Tetracycline (TC) and Cadmium(Cd) concentration ratios (both alone and combined) on rice growth,pollutant accumulation,and transportation during the tillering stage in hydroponic system.Surprisingly,our findings indicated that the interaction between TC and Cd could alleviate the toxic effects of TC/Cd on aerial rice structures and decrease pollutant burdens during root elongation.In contrast,TC and Cd synergistically promoted the accumulation of TC/Cd in rice roots.However,their interaction increased the accumulation of TC in roots while decreasing the accumulation of Cd when the toxicant doses increased.The strong affinity of rice to Cd promoted its upward transport from the roots,whereas the toxic effects of TC reduced TC transport.Therefore,the combined toxicity of the two pollutants inhibited their upward transport.Additionally,a low concentration of TC promoted the accumulation of Cd in rice mainly in the root tip.Furthermore,a certain dose of TC promoted the upward migration of Cd from the root tip.Laser ablation-inductively coupled plasma mass spectrometry demonstrated that Cd mainly accumulated in the epidermis and stele of the root,whereas Fe mainly accumulated in the epidermis,which inhibited the absorption and accumulation of Cd by the rice roots through the generation of a Fe plaque.Our findings thus provide insights into the effects of TC and Cd co-exposure on rice growth.
基金the financial support of the National Natural Science Foundation of China(Nos.21971169,21871194,21572142,21372165 and 21321061)National Key Research and Development Program of China(No.2017YFA0505903)Science & Technology Department of Sichuan Province(Nos.2019YJ0090,2019YJ0160,2017SZ0021)
文摘Water-soluble triplet sensitizer with permethyl-β-cyclodextrin(PMCD)grafting on a Schiff-base Pt(II)complex(Pt-2),in which PMCD unit serves as a host for binding the acceptors and the Schiff-base Pt(II)complex serves as a triplet sensitizer,was synthesized to investigate the effect of supramolecular complexation and assembly on the triplet-triplet annihilation upconversion emission in water.9,10-Diphenylanthracence(DPA)carboxylate(A-1)and its dimer(A-2)in which two DPA carboxylate were covalently linked with an alkyl chain were synthesized as triplet acceptors which also play a role of vip molecules for PMCD.A-1 and A-2 showed high affinity with PMCD,and A-2 can readily aggregate in water and form micron sized assemblies due to the hydrophobic effect andπ-πstacking of anthracene core in A-2.The efficiency of TTA-UC was demonstrated to be enhanced by a synergistic effect of host-vip complexation of Pt-2 with A-2 and the self-aggregation of the acceptor A-2,which facilitated the energy transfer and energy fusion among donor and acceptor.
基金This work was supported by National Research and Development Project of China(grant no.2023YFF0724803)Scientific and technological innovation project of China Academy of Chinese Medical Sciences(grant no.CI2021B015)The Fundamental Research Funds for the Central Public Welfare Research Institutes(grant no.ZZ15-WT-04,ZZ15-WT-08).
文摘Blood deficiency syndrome(BDS)refers to a pathological state with blood dysfunction and organ dystrophy in traditional Chinese medicine.Danggui Wuji granules(DWG)was developed from a formula containing Angelicae Sinensis Radix and Musculus et Os Galli Domestici.Herein,we investigated the mechanism of DWG in treating BDS by modulating gut microbiota.We found that DWG protected mice from BDS by elevating the levels of red blood cell count,hemoglobin,and hematocrit in peripheral blood and increasing the erythrocyte membrane Na+-K+-ATPase activity.Danggui Wuji granules changed the composition and metabolites of colonic flora.Notably,Lactobacillus,Muribaculaceae,and Alistipes were the main genera showing changes after DWG treatment.Our findings revealed that DWG presented a positive therapeutic effect on BDS in mice by regulating the gut microbiota and metabolites.The protective mechanism of DWG was associated with pathways such as metabolic pathways,biosynthesis of secondary metabolites,ABC transporters,ribosome,thyroid hormone synthesis,lysine degradation,galactose metabolism,tyrosine metabolism,etc.
