In tests on dynamic performance of high-speed trains, it is found that there is an unfavorable speed interval for some vehicles, which would he harmful to the daily operation of the vehicle. By analyzing the relations...In tests on dynamic performance of high-speed trains, it is found that there is an unfavorable speed interval for some vehicles, which would he harmful to the daily operation of the vehicle. By analyzing the relationship of vibration frequencies of the vehicle and its running speed, this paper finds that the unfavorable speed interval is caused by the vibration superposition of the natural frequency of the vehicle system with the frequency of external excitation. Taking some electric multiple units （EMUs） as examples, we proposed an approach to obtaining the unfavorable speed interval of vehicles. By analyzing the relation between vibration frequencies and the running speed of the vehicle, the natural frequencies of the vehicle system and the external excitations are distinguished. In the end, we suggest some measures to minimize the negative influences of the unfavorable speed interval, such as shifting frequency, reducing or eliminating external excitation.展开更多
Alzheimer’s disease(AD)is the major form of dementia in the elderly and is closely related to the toxic effects of microglia sustained activation.In AD,sustained microglial activation triggers impaired synaptic pruni...Alzheimer’s disease(AD)is the major form of dementia in the elderly and is closely related to the toxic effects of microglia sustained activation.In AD,sustained microglial activation triggers impaired synaptic pruning,neuroinflammation,neurotoxicity,and cognitive deficits.Accumulating evidence has demonstrated that aberrant expression of deubiquitinating enzymes is associated with regulating microglia function.Here,we use RNA sequencing to identify a deubiquitinase YOD1 as a regulator of microglial function and AD pathology.Further study showed that YOD1 knockout significantly improved the migration,phagocytosis,and inflammatory response of microglia,thereby improving the cognitive impairment of AD model mice.Through LCeMS/MS analysis combined with Co-IP,we found that Myosin heavy chain 9(MYH9),a key regulator maintaining microglia homeostasis,is an interacting protein of YOD1.Mechanistically,YOD1 binds to MYH9 and maintains its stability by removing the K48 ubiquitin chain from MYH9,thereby mediating the microglia polarization signaling pathway to mediate microglia homeostasis.Taken together,our study reveals a specific role of microglial YOD1 in mediating microglia homeostasis and AD pathology,which provides a potential strategy for targeting microglia to treat AD.展开更多
The prevalent oral diseases,such as dental caries,chronic gingivitis,and periodontitis,which are primarily caused by pathogenic bacteria,pose significant public health risks and impose substantial economic burdens.How...The prevalent oral diseases,such as dental caries,chronic gingivitis,and periodontitis,which are primarily caused by pathogenic bacteria,pose significant public health risks and impose substantial economic burdens.However,conventional treatment strategies for oral pathogens rely on mechanical debridement and antibiotic treatment,which remain unsatisfactory and contribute to the emergence of antimicrobial resistance pathogens.The escalating crisis of antibiotic resistance and the intricate microbial communities in oral niches urgently demand innovative antimicrobial strategies that can overcome these issues.Metal-bearing nanomaterials(MBNs),as an integration of metallic components with other substances such as polymers or inorganic materials,have demonstrated improved antimicrobial effectiveness while mitigating the toxicity associated with pure metals in oral environments.This review provides an innovative overview of designing and utilizing MBNs for oral antimicrobial applications,bridging the gap between nanomaterial design and clinical dentistry needs while guiding the development of next-generation antimicrobials in the post-antibiotic era.Firstly,we categorize and elucidate the main antibacterial mechanisms of metallic components in MBNs.Furthermore,a comprehensive summary is provided on the up-to-date advancements in using MBNs for oral antibacterial purposes,highlighting the pivotal role of metals in enhancing antibacterial properties.Finally,we discuss the existing challenges and potential future developments to establish a theoretical foundation for ongoing progress and clinical approval.展开更多
文摘In tests on dynamic performance of high-speed trains, it is found that there is an unfavorable speed interval for some vehicles, which would he harmful to the daily operation of the vehicle. By analyzing the relationship of vibration frequencies of the vehicle and its running speed, this paper finds that the unfavorable speed interval is caused by the vibration superposition of the natural frequency of the vehicle system with the frequency of external excitation. Taking some electric multiple units （EMUs） as examples, we proposed an approach to obtaining the unfavorable speed interval of vehicles. By analyzing the relation between vibration frequencies and the running speed of the vehicle, the natural frequencies of the vehicle system and the external excitations are distinguished. In the end, we suggest some measures to minimize the negative influences of the unfavorable speed interval, such as shifting frequency, reducing or eliminating external excitation.
基金supported by the National Natural Science Foundation of China(21961142009 to Guang Liang and 82360805 to Jinfeng Sun)Zhejiang Provincial Key Scientific Project(2021C03041 to Guang Liang)+1 种基金Natural Science Foundation of Zhejiang province(LQ23H090018 to Xia Zhao)Medical and health Science and Technology Project of Zhejiang Province(2024KY055 to Xia Zhao).
文摘Alzheimer’s disease(AD)is the major form of dementia in the elderly and is closely related to the toxic effects of microglia sustained activation.In AD,sustained microglial activation triggers impaired synaptic pruning,neuroinflammation,neurotoxicity,and cognitive deficits.Accumulating evidence has demonstrated that aberrant expression of deubiquitinating enzymes is associated with regulating microglia function.Here,we use RNA sequencing to identify a deubiquitinase YOD1 as a regulator of microglial function and AD pathology.Further study showed that YOD1 knockout significantly improved the migration,phagocytosis,and inflammatory response of microglia,thereby improving the cognitive impairment of AD model mice.Through LCeMS/MS analysis combined with Co-IP,we found that Myosin heavy chain 9(MYH9),a key regulator maintaining microglia homeostasis,is an interacting protein of YOD1.Mechanistically,YOD1 binds to MYH9 and maintains its stability by removing the K48 ubiquitin chain from MYH9,thereby mediating the microglia polarization signaling pathway to mediate microglia homeostasis.Taken together,our study reveals a specific role of microglial YOD1 in mediating microglia homeostasis and AD pathology,which provides a potential strategy for targeting microglia to treat AD.
基金supports from the National Natural Science Foundation of China(32001782,22222402,22474012)Natural Science Foundation of Hunan Province(2024JJ3001)+1 种基金the Excellent Youth Funding of Hunan Provincial Education Department(22B0347)the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province.
文摘The prevalent oral diseases,such as dental caries,chronic gingivitis,and periodontitis,which are primarily caused by pathogenic bacteria,pose significant public health risks and impose substantial economic burdens.However,conventional treatment strategies for oral pathogens rely on mechanical debridement and antibiotic treatment,which remain unsatisfactory and contribute to the emergence of antimicrobial resistance pathogens.The escalating crisis of antibiotic resistance and the intricate microbial communities in oral niches urgently demand innovative antimicrobial strategies that can overcome these issues.Metal-bearing nanomaterials(MBNs),as an integration of metallic components with other substances such as polymers or inorganic materials,have demonstrated improved antimicrobial effectiveness while mitigating the toxicity associated with pure metals in oral environments.This review provides an innovative overview of designing and utilizing MBNs for oral antimicrobial applications,bridging the gap between nanomaterial design and clinical dentistry needs while guiding the development of next-generation antimicrobials in the post-antibiotic era.Firstly,we categorize and elucidate the main antibacterial mechanisms of metallic components in MBNs.Furthermore,a comprehensive summary is provided on the up-to-date advancements in using MBNs for oral antibacterial purposes,highlighting the pivotal role of metals in enhancing antibacterial properties.Finally,we discuss the existing challenges and potential future developments to establish a theoretical foundation for ongoing progress and clinical approval.
