In the application of clean energy heating,the development of a low-carbon winter heat supply in severe cold regions of China is hindered by the stability of heat sources.To ensure the smooth transformation of traditi...In the application of clean energy heating,the development of a low-carbon winter heat supply in severe cold regions of China is hindered by the stability of heat sources.To ensure the smooth transformation of traditional energy to clean energy heating modes,the feasibility of a heating system coupling traditional and clean energies was studied using the heating system of an office building in a cold region of China as the research object.The air-source heat pump(ASHP)heating system used in the office building in this study was field-tested.The problems existing in the heating system were analysed using testing data combined with the existing conditions of the building.Solar-air source heat pump coupled heating system based on heat grid(NH-SASHP)was proposed,and the system model was established using TRNSYS software.The operation effect of the NH-SASHP coupling system was analysed,and the control strategy of the coupling system was optimized.The results showed that NH-SASHP system possessed certain advantages over the ASHP system during the heating season,and the energy saving rate is 50.79%compared with the ASHP system.Under the most unfavourable working conditions in the middle of severe cold,the indoor temperature compliance rate was 100%.The average coefficient of performance of the heat pump system(COPsys)of NH-SASHP system and ASHP system were 6.27 and 3.55,respectively.The operating cost of the NH-SASHP system is approximately 72.3%of the ASHP system.展开更多
Background:Nuclear Yes1-associated transcriptional regulator(YAP1)promotes tumor progression.However,the function of cytoplasmic YAP1 in breast cancer cells and its impact on the survival of breast cancer patients rem...Background:Nuclear Yes1-associated transcriptional regulator(YAP1)promotes tumor progression.However,the function of cytoplasmic YAP1 in breast cancer cells and its impact on the survival of breast cancer patients remain unclear.Our research aimed to explore the biological function of cytoplasmic YAP1 in breast cancer cells and the possibility of cytoplasmic YAP1 as a predictive marker of breast cancer survival.Methods:We constructed cell mutant models,including NLS-YAP15SA(nuclear localized),YAP1S94A(incapable of binding to the TEA domain transcription factor family)and YAP1S127D(cytoplasmic localized),and used Cell Counting Kit-8(CCK-8)assays,5-ethynyl-2’-deoxyuridine(EdU)incorporation assays,and Western blotting(WB)analysis to detect cell proliferation and apoptosis.The specific mechanism of cytoplasmic YAP1-mediated endosomal sorting complexes required for transport III(ESCRT-III)assembly was studied by co-immunoprecipitation,immunofluorescence staining,and WB analysis.Epigallocatechin gallate(EGCG)was used to simulate YAP1 retention in the cytoplasm in in vitro and in vivo experiments to study the function of cytoplasmic YAP1.YAP1 binding to NEDD4-like E3 ubiquitin protein ligase(NEDD4L)was identified using mass spectrometry and was verified in vitro.Breast tissue microarrays were used to analyze the relationship between cytoplasmic YAP1 expression and the survival of breast cancer patients.Results:YAP1 was mainly expressed in the cytoplasm in breast cancer cells.Cytoplasmic YAP1 promoted autophagic death of breast cancer cells.Cytoplasmic YAP1 bound to the ESCRT-III complex subunits charged multivesicular body protein 2B(CHMP2B)and vacuolar protein sorting 4 homolog B(VPS4B),promoting assembly of CHMP2B-VPS4B and activating autophagosome formation.EGCG retained YAP1 in the cytoplasm,promoting the assembly of CHMP2B-VPS4B to promote autophagic death of breast cancer cells.YAP1 bound to NEDD4L,and NEDD4L mediated ubiquitination and degradation of YAP1.Breast tissue microarrays revealed that high levels of cytoplasmic YAP1 were beneficial to the survival of breast cancer patients.Conclusions:Cytoplasmic YAP1 mediated autophagic death of breast cancer cells by promoting assembly of the ESCRT-III complex;furthermore,we established a new breast cancer survival prediction model based on cytoplasmic YAP1 expression.展开更多
文摘In the application of clean energy heating,the development of a low-carbon winter heat supply in severe cold regions of China is hindered by the stability of heat sources.To ensure the smooth transformation of traditional energy to clean energy heating modes,the feasibility of a heating system coupling traditional and clean energies was studied using the heating system of an office building in a cold region of China as the research object.The air-source heat pump(ASHP)heating system used in the office building in this study was field-tested.The problems existing in the heating system were analysed using testing data combined with the existing conditions of the building.Solar-air source heat pump coupled heating system based on heat grid(NH-SASHP)was proposed,and the system model was established using TRNSYS software.The operation effect of the NH-SASHP coupling system was analysed,and the control strategy of the coupling system was optimized.The results showed that NH-SASHP system possessed certain advantages over the ASHP system during the heating season,and the energy saving rate is 50.79%compared with the ASHP system.Under the most unfavourable working conditions in the middle of severe cold,the indoor temperature compliance rate was 100%.The average coefficient of performance of the heat pump system(COPsys)of NH-SASHP system and ASHP system were 6.27 and 3.55,respectively.The operating cost of the NH-SASHP system is approximately 72.3%of the ASHP system.
基金National Natural Science Foundation of China,Grant/Award Numbers:81573001,81773295Haiyan Research Fund Project of Harbin Medical University Cancer Hospital,Grant/Award Number:JJZD2023-04Beijing Kechuang Medical Development Foundation,Grant/Award Number:KC2021-JF-0055-06。
文摘Background:Nuclear Yes1-associated transcriptional regulator(YAP1)promotes tumor progression.However,the function of cytoplasmic YAP1 in breast cancer cells and its impact on the survival of breast cancer patients remain unclear.Our research aimed to explore the biological function of cytoplasmic YAP1 in breast cancer cells and the possibility of cytoplasmic YAP1 as a predictive marker of breast cancer survival.Methods:We constructed cell mutant models,including NLS-YAP15SA(nuclear localized),YAP1S94A(incapable of binding to the TEA domain transcription factor family)and YAP1S127D(cytoplasmic localized),and used Cell Counting Kit-8(CCK-8)assays,5-ethynyl-2’-deoxyuridine(EdU)incorporation assays,and Western blotting(WB)analysis to detect cell proliferation and apoptosis.The specific mechanism of cytoplasmic YAP1-mediated endosomal sorting complexes required for transport III(ESCRT-III)assembly was studied by co-immunoprecipitation,immunofluorescence staining,and WB analysis.Epigallocatechin gallate(EGCG)was used to simulate YAP1 retention in the cytoplasm in in vitro and in vivo experiments to study the function of cytoplasmic YAP1.YAP1 binding to NEDD4-like E3 ubiquitin protein ligase(NEDD4L)was identified using mass spectrometry and was verified in vitro.Breast tissue microarrays were used to analyze the relationship between cytoplasmic YAP1 expression and the survival of breast cancer patients.Results:YAP1 was mainly expressed in the cytoplasm in breast cancer cells.Cytoplasmic YAP1 promoted autophagic death of breast cancer cells.Cytoplasmic YAP1 bound to the ESCRT-III complex subunits charged multivesicular body protein 2B(CHMP2B)and vacuolar protein sorting 4 homolog B(VPS4B),promoting assembly of CHMP2B-VPS4B and activating autophagosome formation.EGCG retained YAP1 in the cytoplasm,promoting the assembly of CHMP2B-VPS4B to promote autophagic death of breast cancer cells.YAP1 bound to NEDD4L,and NEDD4L mediated ubiquitination and degradation of YAP1.Breast tissue microarrays revealed that high levels of cytoplasmic YAP1 were beneficial to the survival of breast cancer patients.Conclusions:Cytoplasmic YAP1 mediated autophagic death of breast cancer cells by promoting assembly of the ESCRT-III complex;furthermore,we established a new breast cancer survival prediction model based on cytoplasmic YAP1 expression.
