The Bama diet(BD),Mediterranean diet(MD),and Japanese diet(JD)are three typical dietary patterns from distinct geographical regions.The aim of this study was to compare the efficacy of three diets in preventing coliti...The Bama diet(BD),Mediterranean diet(MD),and Japanese diet(JD)are three typical dietary patterns from distinct geographical regions.The aim of this study was to compare the efficacy of three diets in preventing colitis through gut microbe-metabolite-mediated regulatory mechanisms.This was achieved by integrating gut microbiome and metabolomics analyses.Results showed that BD could significantly increase the level of interleukin 10(IL-10),which has the potential to modulate the intestinal immune response.This was achieved by modulating the a-linoleic acid metabolism and promoting Akkermansia proliferation.MD elevated IL-10 level and increased the expression of tight junction proteins such as occludin,claudin-1,and zonula occludens-1.This effect might be attributed to the increased abundance of bifidobacteria,which impacted the primary bile acid biosynthesis pathway.JD decreased tumor necrosis factorαlevel and increased IL-10 level.This could be due to JD promoting the enrichment of Faecalibacterium,which affected the metabolism of arachidonic acid.Furthermore,the anti-inflammatory effects of the different metabolites,namely a-linoleic acid,cholic acid,and arachidonic acid,were confirmed using the RAW264.7 cellular inflammation model.展开更多
Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality traits.Mediator Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,live...Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality traits.Mediator Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,livestock growth,carcass traits,and triglyceride deposition.However,the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown.Methods:This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle.The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western blotting.Results:The MED4 gene had significantly high expression during preadipocyte differentiation(p<0.05).Overexpression of the MED4 gene increased the expression of the PPARγgene,a preadipocyte differentiation biomarker,improved cellular lipid droplets and triglycerides accumulation,and positively accelerated adipocyte maturation(p<0.05).Interference of the MED4 gene can negatively regulate preadipocyte differentiation.Conclusion:This study showed that the MED4 gene may affect the preadipocyte differentiation and adipogenesis in Chinese Red Steppe cattle by regulating the PPARγgene.展开更多
Garvey-Kelson mass relations connect nuclear masses of neighboring nuclei within high accuracy, and provide us with convenient tools in predicting unknown masses by extrapolations from existent experimental data. In t...Garvey-Kelson mass relations connect nuclear masses of neighboring nuclei within high accuracy, and provide us with convenient tools in predicting unknown masses by extrapolations from existent experimental data. In this paper we investigate optimal"channels" of the Garvey-Kelson relations in extrapolation to the unknown regions, and tabulate our predicted masses by using these optimized channels of the Garvey-Kelson relations.展开更多
基金supported by the Key-Area Research and Development Program of Guangdong Province(2021B0707060001)the National Natural Science Foundation of China(32001696)Guangxi Science and Technology Base and Talent Special Project(2021AC19445).
文摘The Bama diet(BD),Mediterranean diet(MD),and Japanese diet(JD)are three typical dietary patterns from distinct geographical regions.The aim of this study was to compare the efficacy of three diets in preventing colitis through gut microbe-metabolite-mediated regulatory mechanisms.This was achieved by integrating gut microbiome and metabolomics analyses.Results showed that BD could significantly increase the level of interleukin 10(IL-10),which has the potential to modulate the intestinal immune response.This was achieved by modulating the a-linoleic acid metabolism and promoting Akkermansia proliferation.MD elevated IL-10 level and increased the expression of tight junction proteins such as occludin,claudin-1,and zonula occludens-1.This effect might be attributed to the increased abundance of bifidobacteria,which impacted the primary bile acid biosynthesis pathway.JD decreased tumor necrosis factorαlevel and increased IL-10 level.This could be due to JD promoting the enrichment of Faecalibacterium,which affected the metabolism of arachidonic acid.Furthermore,the anti-inflammatory effects of the different metabolites,namely a-linoleic acid,cholic acid,and arachidonic acid,were confirmed using the RAW264.7 cellular inflammation model.
基金Supported by the China Agriculture Research System of MOF and MARA(CARS37).Agricultural Science and Technology Innovation Program of Jilin Province CXGC2022DX007.
文摘Background:The regulatory genes related to lipid metabolism affect the intramuscular fat(IMF)content and improve meat quality traits.Mediator Complex Subunit 4(MED4),a vitamin D receptor protein,affects vitamin D,livestock growth,carcass traits,and triglyceride deposition.However,the physiological function of the MED4 gene on bovine adipocyte differentiation remains unknown.Methods:This study explored the function of the MED4 gene in preadipocyte differentiation in Chinese Red Steppe cattle.The overexpression plasmid and the interference sequences of the MED4 gene were constructed to detect the effects of the MED4 gene on adipogenesis and biomakers using quantitative polymerase chain reaction and western blotting.Results:The MED4 gene had significantly high expression during preadipocyte differentiation(p<0.05).Overexpression of the MED4 gene increased the expression of the PPARγgene,a preadipocyte differentiation biomarker,improved cellular lipid droplets and triglycerides accumulation,and positively accelerated adipocyte maturation(p<0.05).Interference of the MED4 gene can negatively regulate preadipocyte differentiation.Conclusion:This study showed that the MED4 gene may affect the preadipocyte differentiation and adipogenesis in Chinese Red Steppe cattle by regulating the PPARγgene.
基金supported by the National Natural Science Foundation of China(Grant Nos.11225524,11675101,and 11505113)the National Basic Research Program of China(Grant No.2013CB834401)+1 种基金Shanghai Key Laboratory(Grant No.11DZ2260700)the Program of Shanghai Academic/Technology Research Leader(Grant No.16XD1401600)
文摘Garvey-Kelson mass relations connect nuclear masses of neighboring nuclei within high accuracy, and provide us with convenient tools in predicting unknown masses by extrapolations from existent experimental data. In this paper we investigate optimal"channels" of the Garvey-Kelson relations in extrapolation to the unknown regions, and tabulate our predicted masses by using these optimized channels of the Garvey-Kelson relations.
