In order to reduce the occurrence of soil-borne diseases,such as tomato bacterial wilt and Fusarium wilt,and to improve the yield of cherry tomato in Hainan,the paper had summarized grafting and seedling technology of...In order to reduce the occurrence of soil-borne diseases,such as tomato bacterial wilt and Fusarium wilt,and to improve the yield of cherry tomato in Hainan,the paper had summarized grafting and seedling technology of cherry tomato from the aspects of rootstock selection,rootstock seedling cultivation,scion seedling cultivation,preparation before grafting,grafting method and seedling management after grafting.It was found that the technique could significantly improve the yield and disease resistance of cherry tomato.展开更多
Rapid and accurate intraoperative pathological diagnosis(IOPD)is essential for intraoperative decision-making to improve patients'outcomes and avoid reoperations.In this study,using a NAD(P)H-activated fluorescent...Rapid and accurate intraoperative pathological diagnosis(IOPD)is essential for intraoperative decision-making to improve patients'outcomes and avoid reoperations.In this study,using a NAD(P)H-activated fluorescent probe,a multifunctional fluorescent indicator has been developed to selectively identify tumor cells from normal tissue and to achieve cancer grading identification.This rapid response probe,CyQ-1,features unprecedented sensitivity and rapid response toward NADH at low nanomolar levels under physiological conditions.Moreover,this indicator allows both colorimetric and fluorescent NADH detection in HeLa,A549,MDA-MB-231,4T1,MCF-7,HePG2,HUVEC,and HL-7702 cells.Expanding the use of this indicator to advanced tissue models,its ability to visualize NADH in 120 paraffin-embedded colorectal sections and 20 cases of intraoperative frozen sections of lung cancer was further verified.CyQ-1-based cancer grading identification shows an overall 92.5 and 100%agreement with the“gold standard test”of histologic grading toward paraffin and frozen sections,respectively.The sensitivity and specificity for discriminating poorly,moderately,and well-differentiated tumor sections were all above 90%.In a word,the rapid and accurate NADH detection ability for clinical sections makes this proposed indicator a potential candidate for clinical IOPD quantification and tumor differentiation grade recognition.展开更多
基金Supported by Science and Technology Project of Hainan Province(ZDYF2020055)。
文摘In order to reduce the occurrence of soil-borne diseases,such as tomato bacterial wilt and Fusarium wilt,and to improve the yield of cherry tomato in Hainan,the paper had summarized grafting and seedling technology of cherry tomato from the aspects of rootstock selection,rootstock seedling cultivation,scion seedling cultivation,preparation before grafting,grafting method and seedling management after grafting.It was found that the technique could significantly improve the yield and disease resistance of cherry tomato.
基金supported by the National Natural Science Foundation of China(31901045,21877083,31901056,62005132)Excellent Youth Foundation of Jiangsu Scientific Committee(BK20220060)Jiangsu Specially-Appointed Professor(06200048,06200053).
文摘Rapid and accurate intraoperative pathological diagnosis(IOPD)is essential for intraoperative decision-making to improve patients'outcomes and avoid reoperations.In this study,using a NAD(P)H-activated fluorescent probe,a multifunctional fluorescent indicator has been developed to selectively identify tumor cells from normal tissue and to achieve cancer grading identification.This rapid response probe,CyQ-1,features unprecedented sensitivity and rapid response toward NADH at low nanomolar levels under physiological conditions.Moreover,this indicator allows both colorimetric and fluorescent NADH detection in HeLa,A549,MDA-MB-231,4T1,MCF-7,HePG2,HUVEC,and HL-7702 cells.Expanding the use of this indicator to advanced tissue models,its ability to visualize NADH in 120 paraffin-embedded colorectal sections and 20 cases of intraoperative frozen sections of lung cancer was further verified.CyQ-1-based cancer grading identification shows an overall 92.5 and 100%agreement with the“gold standard test”of histologic grading toward paraffin and frozen sections,respectively.The sensitivity and specificity for discriminating poorly,moderately,and well-differentiated tumor sections were all above 90%.In a word,the rapid and accurate NADH detection ability for clinical sections makes this proposed indicator a potential candidate for clinical IOPD quantification and tumor differentiation grade recognition.
