The majors accredited by the Engineering Education Accreditation(EEA)reflect the accreditation agency’s recognition of the school’s engineering programs.Excellent accreditation management holds significant importanc...The majors accredited by the Engineering Education Accreditation(EEA)reflect the accreditation agency’s recognition of the school’s engineering programs.Excellent accreditation management holds significant importance for the advancement of engineering education programs.However,the traditional engineering education system framework suffers from the opacity of raw education data and the difficulty for accreditation bodies to forensically examine the self-assessment reports.To solve these issues,an EEA framework based on Hyperledger Fabric blockchain technology is proposed in this work.Firstly,all relevant stakeholders and information interactions occur within the blockchain network,ensuring the authenticity of educational data and enhancing the transparency of accreditation processes.Secondly,multiple roles are abstracted into a single organization to optimize the network topology.The original data is stored off-chain,and the hash values of the data are stored on-chain to reduce on-chain storage costs.The experimental results show that the proposed framework has a high throughput and the network latency of writing data to the blockchain is reduced by at least 0.04 s.It effectively improves network performance and security,which provides new insights for EEA management.展开更多
[Objectives]This study was conducted to optimize the preparation process of Tianlu Tongluo Granules and establish its quality standard.[Methods]Tianlu Tongluo Granules was prepared by an orthogonal test.TLC was used t...[Objectives]This study was conducted to optimize the preparation process of Tianlu Tongluo Granules and establish its quality standard.[Methods]Tianlu Tongluo Granules was prepared by an orthogonal test.TLC was used to identify Radix Astragali preparata,Radix Paeoniae Alba,Scutellariae Radix and Acori Tatarinowii Rhizoma in the granules,and the content of paeoniflorin in the finished product was determined by HPLC.[Results]The extraction process of Tianlu Tongluo Granules was as follows:adding 6 times of water,and decocting twice,1 h/time.In the forming process,the optimal ratio of thick paste to excipients as 10∶8.6,and the optimal ratio of excipients dextrin:starch:colloidal silicon dioxide was 6.5∶0.5∶1.6.The results showed that TLC spots were obvious,the separation was significant,and the specificity was strong.The paeoniflorin content had a good linear relationship in the concentration range of 5.9048-590.48 mg/L(r=0.9999).The average sample recovery was 101.10%,and the RSD was 1.18%.[Conclusions]The preparation process is stable and reasonable,and the established quality control method is specific and reproducible,and can be used for the quality control of Tianlu Tongluo Granules.展开更多
[Objectives]This study was conducted to analyze the medication rules of clinical prescriptions of traditional Chinese medicine decoction pieces for the treatment of novel coronavirus pneumonia(COVID-19)during the epid...[Objectives]This study was conducted to analyze the medication rules of clinical prescriptions of traditional Chinese medicine decoction pieces for the treatment of novel coronavirus pneumonia(COVID-19)during the epidemic in multiple regions based on data mining technology,so as to provide a reference for the treatment of COVID-19 with traditional Chinese medicine.[Methods]The traditional Chinese medicine prescriptions used since the outbreak of COVID-19 in Hubei Province during the fight against the epidemic from February 25,2020 to February 14,2022,the traditional Chinese medicine prescriptions used by Guizhou traditional Chinese medicine expert team aiding Hubei Province,the traditional Chinese medicine prescriptions for rehabilitation and conditioning of patients in Ezhou of Hubei Province after discharge,the traditional Chinese medicine prescriptions for the prevention and treatment of COVID-19 in Guizhou Province,and the traditional Chinese medicine prescriptions for the treatment of COVID-19 collected from the end of 2019 to the present from the Chinese database of CNKI were collected as the data of this study.Excel was used to establish a database and enter it into the TCM inheritance calculation platform V3.5,and the association rules and k-means clustering algorithm were used to analyze the frequency of herbal medicines in prescriptions during the treatment of COVID-19,the frequency of four natures,five flavors,meridian distribution,and drug combinations.[Results]A total of 1859 COVID-19 patients treated with traditional Chinese medicine were included,and the proportion of males was higher than that of females,and middle-aged and elderly people were the most common group.A total of 2170 prescriptions of traditional Chinese medicine were included,involving a total of 383 traditional Chinese medicines.High-frequency medicines included poria,Radix Bupleuri,Radix Scutellariae,Herba Pogostemonis,Fructus Forsythiae,Flos Loniceraeetc.The four natures were mainly concentrated in cold,warm and neutral,and the five flavors were mainly concentrated in bitter,pungent and sweet.The herbal medicines were mainly attributed to the lungs and stomach meridians,and were mainly of heat-clearing,exterior syndrome-relieving and diuresis-promoting and damp-clearing types.A total of 24 high-frequency herbal combinations and 35 association rule were excavated,and 3 types of formulas were obtained by cluster analysis.[Conclusions]The analysis results and medicine combinations obtained in the formulas are consistent with the traditional Chinese medicine treatment theory of COVID-19 caused by wind-heat filth accompanied with damp and toxin.展开更多
[Objectives]This study was conducted to investigate the extraction method of Impatiens balsamina L.(Tougucao),and to optimize the extraction process conditions and parameters.[Methods]Different ethanol concentrations,...[Objectives]This study was conducted to investigate the extraction method of Impatiens balsamina L.(Tougucao),and to optimize the extraction process conditions and parameters.[Methods]Different ethanol concentrations,ethanol amounts and extraction time were investigated by an orthogonal test L_(9)(3^(4))and high performance liquid chromatography(HPLC),and the extraction process parameters were evaluated by the yield of extract and the content of quercetin.[Results]Under the three optimal extraction conditions of impregnation,heating reflux and ultrasound,the extract yield and quercetin contents of I.balsamina were 8.27%,9.50%,7.09%and 1.60,3.05,3.03μg/g,respectively.Heating reflux extraction and ultrasonic extraction showed higher extract yields and quercetin contents of I.balsamina,while the values were lower under the impregnation extraction conditions.The primary and secondary factors were:ethanol amount(C)>ethanol concentration(A)>extraction time(B).The optimal extraction process of I.balsamina was as follows:adding 10 times of 60%ethanol and ultrasonically extracting for 30 min.[Conclusions]The method is simple,convenient,accurate and reliable,and provides a scientific basis for the research on the extraction process of I.balsamina and its preparations.展开更多
[Objectives]This study was conducted to establish the quality standard for Lujing Yiqi Shengxue Pills to provide a basis for its quality control.[Methods]According to the quality requirements in the 2020 edition of Ch...[Objectives]This study was conducted to establish the quality standard for Lujing Yiqi Shengxue Pills to provide a basis for its quality control.[Methods]According to the quality requirements in the 2020 edition of Chinese Pharmacopoeia,the quality of Lujing Yiqi Shengxue Pills was evaluated from the aspects of properties,identification,inspection and content determination.Radix Astragali,Radix Angelicae Sinensis,Macrocephalae Rhizoma stir-fried with bran and Radix Paeoniae Alba in the preparation were identified and studied by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)method was established to determine the content of paeoniflorin in the preparation.[Results]The thin layer spots of Radix Astragali,Radix Angelicae Sinensis,Macrocephalae Rhizoma and Radix Paeoniae Alba were clear and round,and the negative preparations had no interference.The properties,moisture,weight difference,loading difference,dissolution time limit and microbial limit all met the requirements.The regression formula for the quantitative analysis of paeoniflorin in the concentration range of 4.86-194.40μg/ml was:Y=12.0241X+0.8797,R^(2)=0.9999;and the average recovery was 98.9%,and the RSD was 1.7%.[Conclusions]The method has good repeatability,and is stable,feasible,simple and convenient in operation and suitable for quality control of Lujing Yiqi Shengxue Pills.展开更多
[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of C...[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of Chinese Pharmacopoeia, applicability tests were conducted on microbial limit test methods for the above two traditional Chinese medicine preparations by the plate method. [Results] The established methods showed recovery values in the range of 0.5-2.0 for both experimental strains, and the control bacteria could be detected in the experimental group, but not in the negative control group. [Conclusions] The microbial limit test methods were reliable for the two traditional Chinese medicine preparations and could be used for quality control.展开更多
[Objectives]This study was conducted to establish a microbial limit test method for Qingyan Zhisou Powder.[Methods]Applicability tests were carried out according to Microbial Limit Tests of Non-sterile Products,Genera...[Objectives]This study was conducted to establish a microbial limit test method for Qingyan Zhisou Powder.[Methods]Applicability tests were carried out according to Microbial Limit Tests of Non-sterile Products,General Rules 1105-1107 of Part IV of Chinese Pharmacopoeia,2020.[Results]Qingyan Zhisou Powder had a strong bacteriostatic effect on Pseudomonas aeruginosa.After increasing the dilution ratio(1:40),the bacteriostatic effect was obviously eliminated.When the total number of aerobic microbes was determined by the test liquid dilution method(1:40),the recovery values were in the range of 50%-200%;and when using the test liquid(1:10)to determine the total number of mold and yeasts,the recovery ranged from 50%to 200%.Escherichia coli,Salmonella and bile-tolerant Gram-negative bacteria were detected by both the routine method and dilution method in experimental groups.[Conclusions]The microbial limit test of Qingyan Zhisou Powder adopted the test liquid dilution method,and the routine method could be used for the test of Escherichia coli,Salmonella and bile-tolerant Gram-negative bacteria.The applicability test of the method is feasible,with scientific and accurate results,and the method can effectively control the quality of the preparation and is recommended for popularization.展开更多
基金supported by the National Natural Science Foundation of China under Grant 62462009the Guangxi Science and Technology Projects under Grant GuiKeAD24010047+4 种基金the Guangxi Natural Science Foundation under Grant 2022GXNSFFA035028Research Fund of Guangxi Normal University under Grant 2021JC006the AI+Education Research Project of Guangxi Humanities Society Science Development Research Center under Grant ZXZJ202205the Guangxi New Engineering and Technical Disciplines Research and Practice Projects under Grant XGK2022005the 2021 New Engineering and Technical Disciplines Research and Practice Project of Guangxi Normal University.
文摘The majors accredited by the Engineering Education Accreditation(EEA)reflect the accreditation agency’s recognition of the school’s engineering programs.Excellent accreditation management holds significant importance for the advancement of engineering education programs.However,the traditional engineering education system framework suffers from the opacity of raw education data and the difficulty for accreditation bodies to forensically examine the self-assessment reports.To solve these issues,an EEA framework based on Hyperledger Fabric blockchain technology is proposed in this work.Firstly,all relevant stakeholders and information interactions occur within the blockchain network,ensuring the authenticity of educational data and enhancing the transparency of accreditation processes.Secondly,multiple roles are abstracted into a single organization to optimize the network topology.The original data is stored off-chain,and the hash values of the data are stored on-chain to reduce on-chain storage costs.The experimental results show that the proposed framework has a high throughput and the network latency of writing data to the blockchain is reduced by at least 0.04 s.It effectively improves network performance and security,which provides new insights for EEA management.
基金Supported by Project of Guiyang Science and Technology Bureau(ZKHT[2020]-18-4).
文摘[Objectives]This study was conducted to optimize the preparation process of Tianlu Tongluo Granules and establish its quality standard.[Methods]Tianlu Tongluo Granules was prepared by an orthogonal test.TLC was used to identify Radix Astragali preparata,Radix Paeoniae Alba,Scutellariae Radix and Acori Tatarinowii Rhizoma in the granules,and the content of paeoniflorin in the finished product was determined by HPLC.[Results]The extraction process of Tianlu Tongluo Granules was as follows:adding 6 times of water,and decocting twice,1 h/time.In the forming process,the optimal ratio of thick paste to excipients as 10∶8.6,and the optimal ratio of excipients dextrin:starch:colloidal silicon dioxide was 6.5∶0.5∶1.6.The results showed that TLC spots were obvious,the separation was significant,and the specificity was strong.The paeoniflorin content had a good linear relationship in the concentration range of 5.9048-590.48 mg/L(r=0.9999).The average sample recovery was 101.10%,and the RSD was 1.18%.[Conclusions]The preparation process is stable and reasonable,and the established quality control method is specific and reproducible,and can be used for the quality control of Tianlu Tongluo Granules.
基金Supported by Public Health and Epidemic Prevention and Control Project of Guiyang Bureau of Science and Technology([2022]-4-4-5)Guizhou Provincial Key Discipline of Traditional Chinese Medicine and Ethnic Medicine:Clinical Traditional Chinese Medicine(QZYYZDXK(JS)-2023-04).
文摘[Objectives]This study was conducted to analyze the medication rules of clinical prescriptions of traditional Chinese medicine decoction pieces for the treatment of novel coronavirus pneumonia(COVID-19)during the epidemic in multiple regions based on data mining technology,so as to provide a reference for the treatment of COVID-19 with traditional Chinese medicine.[Methods]The traditional Chinese medicine prescriptions used since the outbreak of COVID-19 in Hubei Province during the fight against the epidemic from February 25,2020 to February 14,2022,the traditional Chinese medicine prescriptions used by Guizhou traditional Chinese medicine expert team aiding Hubei Province,the traditional Chinese medicine prescriptions for rehabilitation and conditioning of patients in Ezhou of Hubei Province after discharge,the traditional Chinese medicine prescriptions for the prevention and treatment of COVID-19 in Guizhou Province,and the traditional Chinese medicine prescriptions for the treatment of COVID-19 collected from the end of 2019 to the present from the Chinese database of CNKI were collected as the data of this study.Excel was used to establish a database and enter it into the TCM inheritance calculation platform V3.5,and the association rules and k-means clustering algorithm were used to analyze the frequency of herbal medicines in prescriptions during the treatment of COVID-19,the frequency of four natures,five flavors,meridian distribution,and drug combinations.[Results]A total of 1859 COVID-19 patients treated with traditional Chinese medicine were included,and the proportion of males was higher than that of females,and middle-aged and elderly people were the most common group.A total of 2170 prescriptions of traditional Chinese medicine were included,involving a total of 383 traditional Chinese medicines.High-frequency medicines included poria,Radix Bupleuri,Radix Scutellariae,Herba Pogostemonis,Fructus Forsythiae,Flos Loniceraeetc.The four natures were mainly concentrated in cold,warm and neutral,and the five flavors were mainly concentrated in bitter,pungent and sweet.The herbal medicines were mainly attributed to the lungs and stomach meridians,and were mainly of heat-clearing,exterior syndrome-relieving and diuresis-promoting and damp-clearing types.A total of 24 high-frequency herbal combinations and 35 association rule were excavated,and 3 types of formulas were obtained by cluster analysis.[Conclusions]The analysis results and medicine combinations obtained in the formulas are consistent with the traditional Chinese medicine treatment theory of COVID-19 caused by wind-heat filth accompanied with damp and toxin.
基金Supported by Scientific Research Project of Guizhou University of Traditional Chinese Medicine([2019]64)。
文摘[Objectives]This study was conducted to investigate the extraction method of Impatiens balsamina L.(Tougucao),and to optimize the extraction process conditions and parameters.[Methods]Different ethanol concentrations,ethanol amounts and extraction time were investigated by an orthogonal test L_(9)(3^(4))and high performance liquid chromatography(HPLC),and the extraction process parameters were evaluated by the yield of extract and the content of quercetin.[Results]Under the three optimal extraction conditions of impregnation,heating reflux and ultrasound,the extract yield and quercetin contents of I.balsamina were 8.27%,9.50%,7.09%and 1.60,3.05,3.03μg/g,respectively.Heating reflux extraction and ultrasonic extraction showed higher extract yields and quercetin contents of I.balsamina,while the values were lower under the impregnation extraction conditions.The primary and secondary factors were:ethanol amount(C)>ethanol concentration(A)>extraction time(B).The optimal extraction process of I.balsamina was as follows:adding 10 times of 60%ethanol and ultrasonically extracting for 30 min.[Conclusions]The method is simple,convenient,accurate and reliable,and provides a scientific basis for the research on the extraction process of I.balsamina and its preparations.
基金Supported by Guiyang Science and Technology Planning Project(ZKHT[2020]-18-4)。
文摘[Objectives]This study was conducted to establish the quality standard for Lujing Yiqi Shengxue Pills to provide a basis for its quality control.[Methods]According to the quality requirements in the 2020 edition of Chinese Pharmacopoeia,the quality of Lujing Yiqi Shengxue Pills was evaluated from the aspects of properties,identification,inspection and content determination.Radix Astragali,Radix Angelicae Sinensis,Macrocephalae Rhizoma stir-fried with bran and Radix Paeoniae Alba in the preparation were identified and studied by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)method was established to determine the content of paeoniflorin in the preparation.[Results]The thin layer spots of Radix Astragali,Radix Angelicae Sinensis,Macrocephalae Rhizoma and Radix Paeoniae Alba were clear and round,and the negative preparations had no interference.The properties,moisture,weight difference,loading difference,dissolution time limit and microbial limit all met the requirements.The regression formula for the quantitative analysis of paeoniflorin in the concentration range of 4.86-194.40μg/ml was:Y=12.0241X+0.8797,R^(2)=0.9999;and the average recovery was 98.9%,and the RSD was 1.7%.[Conclusions]The method has good repeatability,and is stable,feasible,simple and convenient in operation and suitable for quality control of Lujing Yiqi Shengxue Pills.
基金Supported by Science and Technology Planning Project of Guiyang CityGuizhou Province (ZKHT [2019]-9-4-15)+2 种基金Green Seedling Research Startup Foundation of The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine (GZEYK-Y[2022]29)Research and Development CenterThe Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine(3040-04020001406)。
文摘[Objectives] This study was conducted to establish microbial limit test methods for traditional Chinese medicine preparations Yunpi Granules and Bupi Qiangli Paste. [Methods] According to General Rules of Part IV of Chinese Pharmacopoeia, applicability tests were conducted on microbial limit test methods for the above two traditional Chinese medicine preparations by the plate method. [Results] The established methods showed recovery values in the range of 0.5-2.0 for both experimental strains, and the control bacteria could be detected in the experimental group, but not in the negative control group. [Conclusions] The microbial limit test methods were reliable for the two traditional Chinese medicine preparations and could be used for quality control.
基金Supported by Traditional Chinese Medicine and Ethnic Minority Medicine Technology Research Subject of Guizhou Administrative Bureau(QZYY-2018-095)。
文摘[Objectives]This study was conducted to establish a microbial limit test method for Qingyan Zhisou Powder.[Methods]Applicability tests were carried out according to Microbial Limit Tests of Non-sterile Products,General Rules 1105-1107 of Part IV of Chinese Pharmacopoeia,2020.[Results]Qingyan Zhisou Powder had a strong bacteriostatic effect on Pseudomonas aeruginosa.After increasing the dilution ratio(1:40),the bacteriostatic effect was obviously eliminated.When the total number of aerobic microbes was determined by the test liquid dilution method(1:40),the recovery values were in the range of 50%-200%;and when using the test liquid(1:10)to determine the total number of mold and yeasts,the recovery ranged from 50%to 200%.Escherichia coli,Salmonella and bile-tolerant Gram-negative bacteria were detected by both the routine method and dilution method in experimental groups.[Conclusions]The microbial limit test of Qingyan Zhisou Powder adopted the test liquid dilution method,and the routine method could be used for the test of Escherichia coli,Salmonella and bile-tolerant Gram-negative bacteria.The applicability test of the method is feasible,with scientific and accurate results,and the method can effectively control the quality of the preparation and is recommended for popularization.
