Osteochondral defects pose an enormous challenge,and no satisfactory therapy is available to date due to the hierarchy of the native tissue consisting of articular cartilage and subchondral bone.Constructing a scaffol...Osteochondral defects pose an enormous challenge,and no satisfactory therapy is available to date due to the hierarchy of the native tissue consisting of articular cartilage and subchondral bone.Constructing a scaffold with biological function and biomimetic structure is the key to achieving a high-quality repair effect.Herein,a natural polymer-based bilayer scaffold with a porous architecture similar to that of osteochondral tissue is designed,involving the transforming growth factor-beta3-liposome-loaded upper layer for superficial cartilage regeneration and the nanohydroxyapatite-coated lower layer for subchondral bone rehabilitation.This research is conducted to evaluate the effects of nanoparticle-modified bilayer scaffold to mimic the hierarchical pro-chondrogenic and proosteogenic microenvironment for the recruited endogenous bone marrow mesenchymal stem cells.The fabricated composites were evaluated for mechanical,physicochemical,biological properties,in vitro and in vivo tissue regeneration potential.Overall,the current bilayer scaffold could regenerate a cartilage-bone integrated tissue with a seamless interfacial integration and exhibited superior tissue repair outcomes compared to other single layer scaffolds based on morphological,radiological and histological evaluation,verifying that this novel graft could be an effective approach to tissue-engineered analogs of cartilage-subchondral bone and offer new therapeutic opportunities for osteochondral defect-associated diseases.展开更多
Obesity is a prevalent chronic disease that has significant negative impacts on humans and our companion animals,including dogs and cats.Obesity occurs with multiple comorbidities,such as diabetes,hypertension,heart d...Obesity is a prevalent chronic disease that has significant negative impacts on humans and our companion animals,including dogs and cats.Obesity occurs with multiple comorbidities,such as diabetes,hypertension,heart disease and osteoarthritis in dogs and cats.A direct link between lipid metabolism dysregulation and obesity-associated diseases has been implicated.However,the understanding of such pathophysiology in companion animals is lim-ited.This review aims to address the role of lipid metabolism in various metabolic disorders associated with obesity,emphasizing the involvement of the gut microbiota.Furthermore,we also discuss the management of obesity,including approaches like nutritional interventions,thus providing novel insights into obesity prevention and treatment for canines and felines.展开更多
Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high prepar...Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high preparation costs,low yields,environmental pollution during synthesis,and challenges in regenerating the adsorbent.This study proposes a novel approach to address these limitations by developing nitrogen-doped three-dimensional(3D)polyvinyl alcohol(PVA)crosslinked graphene sponges(N-PGA)using a cross-linking method with ammonium carbonate.This method offers a relatively mild,environmentally friendly approach.Ammonium carbonate serves as both a reducing and modifying agent,facilitating the formation of the intrinsic structure of N-PGA and acting as a nitrogen source.Meanwhile,PVA is utilized as the cross-linking agent.The results demonstrate that N-PGA exhibits a favorable internal 3D hierarchical porous structure and possesses robust mechanical properties.The measured specific surface area(BET)of N-PGA was as high as406.538 m^(2)·g^(-1),which was favorable for its efficient adsorption of Congo red(CR)dye molecules.At an initial concentration of 50 mg·L^(-1),N-PGA achieved an impressive removal rate of 89.6%and an adsorption capacity of 112 mg·g^(-1)for CR dye.Furthermore,it retained 79%of its initial adsorption capacity after 10 cycles,demonstrating excellent regeneration performance.In summary,the synthesized N-PGA displays remarkable efficacy in the adsorption of CR dye in wastewater,opening up new possibilities for utilizing 3D porous graphene nanomaterials as efficient adsorbents in wastewater treatment.展开更多
Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and conversion.To understand its function in maize,we characterized a maize TPI mutant,zmtpi4.In comparison to the wild...Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and conversion.To understand its function in maize,we characterized a maize TPI mutant,zmtpi4.In comparison to the wild type,zmtpi4 mutants showed altered ear development,reduced kernel weight and starch content,modified starch granule morphology,and altered amylose and amylopectin content.Protein,ATP,and pyruvate contents were reduced,indicating ZmTPI4 was involved in glycolysis.Although subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI,the zmtpi4 mutant showed reduced leaf size and chlorophyll content.Overexpression of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight,suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch content.We conclude that ZmTPI4 functions in maize kernel development,starch synthesis,glycolysis,and photosynthesis.展开更多
The development of maize(Zea mays)kernels is a complex physiological process regulated by numerous genes in a spatially and temporally coordinated manner.However,many regulatory genes involved in this process remain u...The development of maize(Zea mays)kernels is a complex physiological process regulated by numerous genes in a spatially and temporally coordinated manner.However,many regulatory genes involved in this process remain unidentified.In this study,we identified ZmZFP2,a gene encoding a C4HC3-type RING zinc finger protein,which regulates kernel size and weight.This discovery was based on suppression subtractive hybridization from maize endosperm in our previous research.We further investigated the role of ZmZFP2 in regulating kernel development.The zmzfp2-ems mutant exhibited significantly reduced kernel size and weight,accompanied by fewer endosperm cells and altered starch and protein accumulation.CRISPR/Cas9-mediated knockouts and overexpression lines confirmed that ZmZFP2 positively regulates kernel size and weight,with overexpression leading to increased kernel size and weight.Transcriptome analysis revealed that ZmZFP2 regulates genes involved in zeatin biosynthesis,starch metabolism,and protein processing,further supporting its role in kernel development.Additionally,ZmZFP2 was shown to interact with the transcription factor ZmEREB98,implicating it in the gene regulatory network during grain filling.Together,these findings demonstrate that ZmZFP2 is a key regulator of maize kernel size and weight,functioning through its E3 ubiquitin ligase activity and interactions with various metabolic pathways.This study provides novel insights into the genetic regulation of kernel development and presents potential strategies for improving maize yield and quality.展开更多
Dear Editor,Schizophrenia is one of the most complicated and serious psychiatric disorders, and patients often show markedly disrupted structural and functional abnormalities during resting-state imaging scans of the ...Dear Editor,Schizophrenia is one of the most complicated and serious psychiatric disorders, and patients often show markedly disrupted structural and functional abnormalities during resting-state imaging scans of the brain. Patients with firstepisode childhood- and adolescence-onset schizophrenia (CAOS) refers to individuals under the age of 18 years whose first episode of illness had occurred before they were 13 years old [1]. The symptoms are similar to adultonset schizophrenia-auditory hallucinations or language deficits and abnormal cognition. However, CAOS patients often present with more severe psychotic symptoms and have a worse prognosis and treatment effect [2].展开更多
To meet the growing demand for antibacterial implants for bone-implant-associated infection therapy and avoid the adverse effects of secondary surgery,a degradable platform with pH responsiveness and ion-associated an...To meet the growing demand for antibacterial implants for bone-implant-associated infection therapy and avoid the adverse effects of secondary surgery,a degradable platform with pH responsiveness and ion-associated antibacterial properties was constructed.A small amount of Sn added to Mg-1Zn alloy reduces the biocorrosion rate,which can be attributed to Sn participation in outer-layer film formation,significantly reducing the biocorrosion rate and hydrogen evolution rate after implantation in vivo.These Mg alloys,which are susceptible to degradation in the acidic bacterial microenvironment,degrade by releasing Mg,Zn and Sn,producing favorably alkaline and antibacterial conditions.Samples with the composition of Mg-1Zn-0.5Sn were found to be beneficial for promoting initial cell adhesion and proliferation,resulting in improved biocompatibility and biosafety.The biocompatibility of this alloy was confirmed by the healthy behavior of animals and the absence of acute or chronic toxicity in the liver,spleen,and kidneys.Our results demonstrate that Mg-1Zn-0.5Sn is safe for biological systems,enabling its efficacious use in biomedical applications.展开更多
The effects of vegetation restoration on matrix structure and erosion resistance of iron tailings were studied at dump sites in Malanzhuang, Qian'an, Hebei province, China. The restoration process involved soil sp...The effects of vegetation restoration on matrix structure and erosion resistance of iron tailings were studied at dump sites in Malanzhuang, Qian'an, Hebei province, China. The restoration process involved soil spray sowing restoration model with Rhus typhina, soil and iron tailings admixture spray sowing restoration model with Amorpha fruticosa Linn. and six-hole brick restoration model with Pinus tabulaeformis Carriere.–Amorpha fruticosa Linn. mixed-forest, and direct restoration model with Hippophae rhamnoides and Sabina vulgaris. Results show that the composition and distribution of particles and aggregates were closely related to erosion resistance(P < 0.05), indicating that matrix structure of iron tailings play an important role in erosion resistance. The improvement in matrix structure of iron tailings by the different restoration models was in the order of R. typhina soil spray sowing > A. fruticosa soil and iron tailings admixture spray sowing > mixed-forest six-hole brick >H. rhamnoides direct restoration > S. vulgaris direct restoration. The R. typhina soil spray sowing restorationmodel resulted in the greatest improvement in matrix structure of iron tailings, increasing the clay(10.6%) and large particle aggregates(18.7%) contents significantly(P < 0.01). Simultaneously, particle population, grading conditions(Cu= 28.86, Cs= 1.65), and aggregate stability(6.02) were significantly improved. The A. fruticosa soil and iron tailings admixture spray sowing restoration model,which effectively improved particle distribution(Cu-= 8.51, Cs= 1.07), increased the number of large aggregates considerably(9.6%), thereby increasing aggregate stability(6.2). The six-hole brick model significantly increased the number of large aggregates(4.0%) and improved the stability of aggregates(6.2). H. rhamnoides direct restoration improved the stability of aggregates(5.1)but showed no other significant improvements. The effect of S. vulgaris direct restoration on matrix structure of iron tailings was not significant. Due to its dependence on matrix structure of iron tailings, the erosion resistance of R.typhina soil spray sowing restoration model was the greatest, while that of S. vulgaris direct restoration was the weakest. There was no significant difference in the erosion resistance of the other models. Overall, vegetation restoration supplemented by soil spray sowing restoration and engineering measures is superior to in situ direct vegetation restoration in the short-term. In-situ direct restoration has long-term ecological significance because of its advanced concept of near-natural restoration and the advantages of low cost, easy operation, and low secondary damage.展开更多
Recently,the effectiveness of neural networks,especially convolutional neural networks,has been validated in the field of natural language processing,in which,sentiment classification for online reviews is an importan...Recently,the effectiveness of neural networks,especially convolutional neural networks,has been validated in the field of natural language processing,in which,sentiment classification for online reviews is an important and challenging task.Existing convolutional neural networks extract important features of sentences without local features or the feature sequence.Thus,these models do not perform well,especially for transition sentences.To this end,we propose a Piecewise Pooling Convolutional Neural Network(PPCNN)for sentiment classification.Firstly,with a sentence presented by word vectors,convolution operation is introduced to obtain the convolution feature map vectors.Secondly,these vectors are segmented according to the positions of transition words in sentences.Thirdly,the most significant feature of each local segment is extracted using max pooling mechanism,and then the different aspects of features can be extracted.Specifically,the relative sequence of these features is preserved.Finally,after processed by the dropout algorithm,the softmax classifier is trained for sentiment classification.Experimental results show that the proposed method PPCNN is effective and superior to other baseline methods,especially for datasets with transition sentences.展开更多
Considering current technology limitation in manufacturing present pin fin heat sinks, a new fabric heat sink has been previously designed. However, there is a lack of an understanding of the heat transferring perform...Considering current technology limitation in manufacturing present pin fin heat sinks, a new fabric heat sink has been previously designed. However, there is a lack of an understanding of the heat transferring performance of this new kind of heat sink. Nowadays, finite element analysis has been generally developed for determining heat transfer from in-line and staggered pin fin heat sinks used in electronic packaging applications. In this study, this method is used to predict the heat transfer performance of the new heat sink with woven fabric structure, called fabric pin fin heat sink. Effect of the fin length and the material types made of heat sink on the thermal-structure response of the pin fin was investigated under forced convection. The results show that the minimum temperature of heat sink decreases with an increase of pin fin length,but the decreasing amplitude has decreased. Moreover, the heat transfer performance of fabric heat sink made of continuous carbon Fibers/Polymer (PPS) is worse than that of copper and of aluminum.展开更多
Calotropis gigantea fiber(CGF)is to a type of seed fiber collected from the fruits of the Calotropis gigantea(CG).From a sustainable biomass resource,CGF is a naturally abundant cellulose fiber with good economic valu...Calotropis gigantea fiber(CGF)is to a type of seed fiber collected from the fruits of the Calotropis gigantea(CG).From a sustainable biomass resource,CGF is a naturally abundant cellulose fiber with good economic value,excellent biocompatibility and biodegradability.CGF has a high hollow structure and is lightweight,which means CGF has received increasing attention in recent years as the starting material for fabrication of different types of functional materials.In this paper,we have reviewed the morphology and characteristics of CGF,including its biological properties,chemical composition,and physical properties.Additionally,we provide an overview of the recent advancements in the extraction process,toughening treatment process,spinning process,and weaving process of CGF,which have garnered significant attention from researchers in the field.The applications of CGF in the fields of environment processing,antibacterial,and smart materials are also summarized.Finally,the challenges and development prospects of CGF are introduced with the ultimate goal of implementing its potential in real-world applications.展开更多
China faces the dual challenge of population aging and rising disability rates,creating an“aging-disability overlap”that places unprecedented pressure on the nation’s healthcare system.This paper analyzes the compl...China faces the dual challenge of population aging and rising disability rates,creating an“aging-disability overlap”that places unprecedented pressure on the nation’s healthcare system.This paper analyzes the complex mechanisms underlying the intersection of aging and disability,revealing that chronic and degenerative diseases are primary contributors to disability among older adults,with disability rates significantly increasing with age and comorbidities.Despite China’s implementation of long-term care insurance pilot programs and community-based elderly care services,multiple challenges persist:insufficient financing sustainability,inconsistent assessment standards,regional development imbalances,professional talent shortages,and social-cultural prejudices.In response,this paper proposes a comprehensive strategy framework aligned with“Healthy China 2030”’s goals of strengthening disability prevention and early intervention mechanisms;enhancing long-term care services with diversified security systems;fostering professional talent development in geriatric and rehabilitation medicine;promoting research and application of intelligent assistive technologies;and creating barrierfree communities within an inclusive social environment.Through these coordinated approaches,we aim to improve the quality of life for older adults with disabilities and facilitate dignified and highquality healthy aging.展开更多
Introduction:As China’s population rapidly ages,concerns have emerged about whether increased longevity among older adults is accompanied by improvements in health status.Methods:This study analyzed data from the 201...Introduction:As China’s population rapidly ages,concerns have emerged about whether increased longevity among older adults is accompanied by improvements in health status.Methods:This study analyzed data from the 2010 and 2020 Chinese censuses to estimate healthy life expectancy(HLE)at age 60 and examined changes in urban-rural disparities and their driving factors.We applied the Sullivan method to estimate gender-and residence-specific HLE,while using the continuous change decomposition method to analyze how mortality and health status contributed to urban-rural differences.Results:Between 2010 and 2020,both the absolute years of HLE and its proportion of total life expectancy increased,with the urban-rural gap narrowing over time.However,the primary driver of urban-rural HLE disparities has shifted from mortality levels to health status,a pattern more pronounced among men.Decomposition analysis further reveals that compared to 2010,the key age groups contributing to urbanrural HLE disparities in 2020 have shifted to older ages,reflecting a transition in health inequalities as the population ages.These findings suggest that while HLE among China’s older population has improved,the nature of health inequalities is evolving.Conclusions:Future public health policies should place greater emphasis on addressing non-fatal health conditions,particularly in rural areas and among the oldest-old,by improving the accessibility and quality of health services to promote healthy aging and reduce urban-rural health disparities.展开更多
苋科(Amaranthaceaesensulato)是石竹目(Caryophyllales)第二大科,目前被普遍接受的苋科为其广义概念,含狭义苋科(Amaranthaceae sensu stricto)和藜科(Chenopodiaceae)。然而到目前为止,藜科是否应作为独立的科还存在争议。此外,广义...苋科(Amaranthaceaesensulato)是石竹目(Caryophyllales)第二大科,目前被普遍接受的苋科为其广义概念,含狭义苋科(Amaranthaceae sensu stricto)和藜科(Chenopodiaceae)。然而到目前为止,藜科是否应作为独立的科还存在争议。此外,广义苋科内部各亚科之间的系统关系也尚未厘清。对广义苋科所有13个亚科代表类群进行取样(共59种),基于8个叶绿体序列片段重建其系统发育关系,并结合分子钟估算,对该科及其主要分支的起源与分化时间进行推测。结果表明,广义苋科与狭义苋科都是很好的单系,但藜科并非单系,因此不支持藜科在科级水平的地位,支持广义苋科的观点。除了多节草亚科(Polycnemoideae)之外,其它亚科的系统位置均得到很好的分辨。分子钟估算结果表明,广义苋科于白垩纪晚期约69.9Ma分化出该科的2个主要分支,且该科在白垩纪-古近纪边界附近时期(约66.0Ma)可能发生过快速辐射分化事件。展开更多
With the rapid development of social economy and urban−rural integration,the phenomenon of farmland aban-donment worldwide has proved to be one of the main trends of land use and land cover change(LUCC),and profoundly...With the rapid development of social economy and urban−rural integration,the phenomenon of farmland aban-donment worldwide has proved to be one of the main trends of land use and land cover change(LUCC),and profoundly affected the rural landscape and regional ecological environment.Restricted by the natural environ-ment,economic development and backward agricultural technology,the phenomenon of farmland abandonment is also common in the Qingzang Plateau(QP).Therefore,this paper adopted the spatial autocorrelation method to analyze the spatial pattern of abandonment in the agricultural and pastoral areas of the Qingzang Plateau(APA−QP)in 2017,and the geographically weighted regression(GWR)model to explore the effects of geograph-ical resources,socio-economic development and location conditions on farmland abandonment.This study found that:1)From 2015 to 2017,the abandoned farmland area in the APA−QP was approximately 18.23×10^(4)ha,with an overall abandonment rate of 15.18%.On the whole,it showed the distribution characteristics of“strong in the south and weak in the north,strong in the east and weak in the west”.2)There were positive spatial correlation between both abandoned area and abandonment rate in the APA−QP,showing“concave”and“convex”patterns,respectively,mainly concentrated in the Huang−shui Valley and the Southeast Tibet;while in the western and northern regions,the degree of abandonment was relatively low.3)Farmland abandonment in the APA−QP was mainly driven by the geographical environment changes and farmers’decision−making on farmland utilization.There was significant spatial heterogeneity on farmland abandonment associated with the impact of geographical resources,socio−economics and location conditions.The geographical resource factors had a positive impact on the abandonment,and were strongly constrained by natural geographic conditions such as altitude and slope.The farmland resources in the Qingzang Plateau are limited,but are of strategic significance for the sustainable development of agriculture in the whole Qingzang Plateau.In order to realize the rational distribution of agricul-ture and animal husbandry and the sustainable utilization of farmland resources in the plateau region,land use strategies should be implemented according to regional differences and regional advantages in order to ensure the ecological environment security of Qingzang Plateau.展开更多
Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to China.The genus has been widely accepted since it was described in 1998,however its phylogenetic position within Caryophyllaceae has never been studie...Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to China.The genus has been widely accepted since it was described in 1998,however its phylogenetic position within Caryophyllaceae has never been studied.In the present study,the whole plastid genome and nuclear ribosomal internal transcribed spacer(ITS)sequences of Pseudocerastium stellarioides was obtained through genome skimming,and the phylogenetic position of the species was studied for the first time.Plastid phylogenomic analysis of Caryophyllaceae revealed that Pseudocerastium is clustered within the tribe Alsineae with strong support.Phylogenetic analyses based on an enlarged taxon sampling of Alsineae using five DNA regions(matK,rbcL,rps16 intron,trnL-F and ITS)revealed that P.stellarioides was nested deeply within Cerastium with strong support.Analyses of morphological character evolution suggest that the ancestral states in Alsineae include three styles and a six-lobed capsule at the apex,while both Cerastium and Pseudocerastium have five styles and ten lobes at the apex of the capsule,further supporting their close relationship.The species Pseudocerastium stellarioides is similar to Cerastium wilsonii in morphology,but differs in having villous indumentum on the lower part of the filaments and compressed globose seeds.Therefore,based on the present molecular and morphological evidence,the generic name Pseudocerastium is reduced here as a new synonym of Cerastium and the species P.stellarioides is transferred to Cerastium as C.jiuhuashanense.展开更多
Auditing is a kind of practical economical subject, with a strong practical and operational. It is the backbone course of accounting major and plays a crucial role in the professional cultivation. But recently, in sta...Auditing is a kind of practical economical subject, with a strong practical and operational. It is the backbone course of accounting major and plays a crucial role in the professional cultivation. But recently, in stage of our country university education, audition＇ s teaching effectiveness is almost the lowest in all the core curriculum of accounting professional. Students generally reflect that Auditing is difficult to learn and abstract, while the teachers of auditing courses also reflect students have low interest in auditing learning, meanwhile there is less relevant case, fewer choices and other materials. This paper analyzes the problems and limitations of the auditing teaching, proposes measures about the teaching reform of university auditing, and finally makes our own suggestions about the reform measures of our university auditing teaching research.展开更多
基金supported by grants from the China Postdoctoral Science Foundation(Nos.2022TQ0397,2022MD723744,2022M710564,2022M720603)Natural Science Foundation of China(Nos.82272553,82102571,81974346,8210257,82472404)+8 种基金Chongqing Municipal Medical Youth Talent Support Program,Chongqing,China(No.YXQN202408)Natural Science Foundation of Chongqing,China(Nos.CSTB2022NSCQ-MSX0089,CSTB2022NSCQ-MSX0104,CSTB2024NSCQMSX0532)Joint Medical Research Project of Health Commission&Science and Technology Bureau of Chongqing,China(No.2024QNXM032)Special Project for the Central Government to Guide the Development of Local Science and Technology in Sichuan Province(No.2023ZYD0071)National Natural Science Foundation of Sichuan(No.24NSFSC1274)Project of Innovative Science Research for Postgraduate of Chongqing Municipal Education Committee,Chongqing,China(Nos.CYS22389,CYB240224)National Natural Science Foundation of Sichuan(No.2024NSFSC0678)Research Project of the Affiliated Hospital of North Sichuan Medical College(Nos.2023ZD002,2023-2ZD001,2024JB001)Disciplines Construction Program of The Third Affiliated Hospital of Chongqing Medical University(Nos.KY23035,KY23041).
文摘Osteochondral defects pose an enormous challenge,and no satisfactory therapy is available to date due to the hierarchy of the native tissue consisting of articular cartilage and subchondral bone.Constructing a scaffold with biological function and biomimetic structure is the key to achieving a high-quality repair effect.Herein,a natural polymer-based bilayer scaffold with a porous architecture similar to that of osteochondral tissue is designed,involving the transforming growth factor-beta3-liposome-loaded upper layer for superficial cartilage regeneration and the nanohydroxyapatite-coated lower layer for subchondral bone rehabilitation.This research is conducted to evaluate the effects of nanoparticle-modified bilayer scaffold to mimic the hierarchical pro-chondrogenic and proosteogenic microenvironment for the recruited endogenous bone marrow mesenchymal stem cells.The fabricated composites were evaluated for mechanical,physicochemical,biological properties,in vitro and in vivo tissue regeneration potential.Overall,the current bilayer scaffold could regenerate a cartilage-bone integrated tissue with a seamless interfacial integration and exhibited superior tissue repair outcomes compared to other single layer scaffolds based on morphological,radiological and histological evaluation,verifying that this novel graft could be an effective approach to tissue-engineered analogs of cartilage-subchondral bone and offer new therapeutic opportunities for osteochondral defect-associated diseases.
基金funded by research grants from the Jiangsu Provincial Double-Innovation Team Program(JSSCTD202147)Nutrition and Care of Maternal and Child Research Fund Project of the Biostime Institute of Nutrition and Care(Grant No.2022BINCMCF006)+1 种基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_3527)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘Obesity is a prevalent chronic disease that has significant negative impacts on humans and our companion animals,including dogs and cats.Obesity occurs with multiple comorbidities,such as diabetes,hypertension,heart disease and osteoarthritis in dogs and cats.A direct link between lipid metabolism dysregulation and obesity-associated diseases has been implicated.However,the understanding of such pathophysiology in companion animals is lim-ited.This review aims to address the role of lipid metabolism in various metabolic disorders associated with obesity,emphasizing the involvement of the gut microbiota.Furthermore,we also discuss the management of obesity,including approaches like nutritional interventions,thus providing novel insights into obesity prevention and treatment for canines and felines.
基金supported by the National Natural Science Foundation of China(51671052,51750110513,52250610222)the Fundamental Research Funds for the Central Universities(N182502042)the Liao Ning Revitilization Talents Program(XLYC1902105)。
文摘Graphene-based materials possess significant potential for the treatment of dye wastewater due to their exceptional adsorption properties toward stubborn pollutants.However,their utilization is hindered by high preparation costs,low yields,environmental pollution during synthesis,and challenges in regenerating the adsorbent.This study proposes a novel approach to address these limitations by developing nitrogen-doped three-dimensional(3D)polyvinyl alcohol(PVA)crosslinked graphene sponges(N-PGA)using a cross-linking method with ammonium carbonate.This method offers a relatively mild,environmentally friendly approach.Ammonium carbonate serves as both a reducing and modifying agent,facilitating the formation of the intrinsic structure of N-PGA and acting as a nitrogen source.Meanwhile,PVA is utilized as the cross-linking agent.The results demonstrate that N-PGA exhibits a favorable internal 3D hierarchical porous structure and possesses robust mechanical properties.The measured specific surface area(BET)of N-PGA was as high as406.538 m^(2)·g^(-1),which was favorable for its efficient adsorption of Congo red(CR)dye molecules.At an initial concentration of 50 mg·L^(-1),N-PGA achieved an impressive removal rate of 89.6%and an adsorption capacity of 112 mg·g^(-1)for CR dye.Furthermore,it retained 79%of its initial adsorption capacity after 10 cycles,demonstrating excellent regeneration performance.In summary,the synthesized N-PGA displays remarkable efficacy in the adsorption of CR dye in wastewater,opening up new possibilities for utilizing 3D porous graphene nanomaterials as efficient adsorbents in wastewater treatment.
基金supported by the Major Public Welfare Projects of Henan Province(201300111100 to Yuling Li)Zhongyuan Scholars in Henan Province(22400510003 to Yuling Li)+2 种基金Tackle Program of Agricultural Seed in Henan Province(2022010201 to Yuling Li)Technical System of Maize Industry in Henan Province(HARS-2202-S to Yuling Li)State Key Laboratory of Wheat and Maize Crop Science(SKL2023ZZ05)。
文摘Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and conversion.To understand its function in maize,we characterized a maize TPI mutant,zmtpi4.In comparison to the wild type,zmtpi4 mutants showed altered ear development,reduced kernel weight and starch content,modified starch granule morphology,and altered amylose and amylopectin content.Protein,ATP,and pyruvate contents were reduced,indicating ZmTPI4 was involved in glycolysis.Although subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI,the zmtpi4 mutant showed reduced leaf size and chlorophyll content.Overexpression of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight,suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch content.We conclude that ZmTPI4 functions in maize kernel development,starch synthesis,glycolysis,and photosynthesis.
基金supported by the National Natural Science Foundation of China(31971962,31771812,and 32272129 to Yuling Li)Zhongyuan Scholars in Henan Province(22400510003 to Yuling Li)+3 种基金the Major Public Welfare Projects of Henan Province(201300111100 to Yuling Li)Tackle Program of Agricultural Seed in Henan Province(2022010201 to Yuling Li)Technical System of Maize Industry in Henan Province(HARS62922-02-S to Yuling Li)Key Scientific Research Projects for Higher Education of Henan Province(19zx001 to Yuling Li).
文摘The development of maize(Zea mays)kernels is a complex physiological process regulated by numerous genes in a spatially and temporally coordinated manner.However,many regulatory genes involved in this process remain unidentified.In this study,we identified ZmZFP2,a gene encoding a C4HC3-type RING zinc finger protein,which regulates kernel size and weight.This discovery was based on suppression subtractive hybridization from maize endosperm in our previous research.We further investigated the role of ZmZFP2 in regulating kernel development.The zmzfp2-ems mutant exhibited significantly reduced kernel size and weight,accompanied by fewer endosperm cells and altered starch and protein accumulation.CRISPR/Cas9-mediated knockouts and overexpression lines confirmed that ZmZFP2 positively regulates kernel size and weight,with overexpression leading to increased kernel size and weight.Transcriptome analysis revealed that ZmZFP2 regulates genes involved in zeatin biosynthesis,starch metabolism,and protein processing,further supporting its role in kernel development.Additionally,ZmZFP2 was shown to interact with the transcription factor ZmEREB98,implicating it in the gene regulatory network during grain filling.Together,these findings demonstrate that ZmZFP2 is a key regulator of maize kernel size and weight,functioning through its E3 ubiquitin ligase activity and interactions with various metabolic pathways.This study provides novel insights into the genetic regulation of kernel development and presents potential strategies for improving maize yield and quality.
基金supported by the National Program for Science and Technology Development of Henan(182102310155)the National Natural Science Foundation of China(81671330)
文摘Dear Editor,Schizophrenia is one of the most complicated and serious psychiatric disorders, and patients often show markedly disrupted structural and functional abnormalities during resting-state imaging scans of the brain. Patients with firstepisode childhood- and adolescence-onset schizophrenia (CAOS) refers to individuals under the age of 18 years whose first episode of illness had occurred before they were 13 years old [1]. The symptoms are similar to adultonset schizophrenia-auditory hallucinations or language deficits and abnormal cognition. However, CAOS patients often present with more severe psychotic symptoms and have a worse prognosis and treatment effect [2].
文摘To meet the growing demand for antibacterial implants for bone-implant-associated infection therapy and avoid the adverse effects of secondary surgery,a degradable platform with pH responsiveness and ion-associated antibacterial properties was constructed.A small amount of Sn added to Mg-1Zn alloy reduces the biocorrosion rate,which can be attributed to Sn participation in outer-layer film formation,significantly reducing the biocorrosion rate and hydrogen evolution rate after implantation in vivo.These Mg alloys,which are susceptible to degradation in the acidic bacterial microenvironment,degrade by releasing Mg,Zn and Sn,producing favorably alkaline and antibacterial conditions.Samples with the composition of Mg-1Zn-0.5Sn were found to be beneficial for promoting initial cell adhesion and proliferation,resulting in improved biocompatibility and biosafety.The biocompatibility of this alloy was confirmed by the healthy behavior of animals and the absence of acute or chronic toxicity in the liver,spleen,and kidneys.Our results demonstrate that Mg-1Zn-0.5Sn is safe for biological systems,enabling its efficacious use in biomedical applications.
基金financed by the National Science and Technology Program in Rural Areas in 12th Five-Year(No.2012AA101403-32012)the High Level Talents Program in Hebei Province(B2014010004)the Provincial Financial Forestry Science and Technology Popularization Program in Hebei Province(201705201)。
文摘The effects of vegetation restoration on matrix structure and erosion resistance of iron tailings were studied at dump sites in Malanzhuang, Qian'an, Hebei province, China. The restoration process involved soil spray sowing restoration model with Rhus typhina, soil and iron tailings admixture spray sowing restoration model with Amorpha fruticosa Linn. and six-hole brick restoration model with Pinus tabulaeformis Carriere.–Amorpha fruticosa Linn. mixed-forest, and direct restoration model with Hippophae rhamnoides and Sabina vulgaris. Results show that the composition and distribution of particles and aggregates were closely related to erosion resistance(P < 0.05), indicating that matrix structure of iron tailings play an important role in erosion resistance. The improvement in matrix structure of iron tailings by the different restoration models was in the order of R. typhina soil spray sowing > A. fruticosa soil and iron tailings admixture spray sowing > mixed-forest six-hole brick >H. rhamnoides direct restoration > S. vulgaris direct restoration. The R. typhina soil spray sowing restorationmodel resulted in the greatest improvement in matrix structure of iron tailings, increasing the clay(10.6%) and large particle aggregates(18.7%) contents significantly(P < 0.01). Simultaneously, particle population, grading conditions(Cu= 28.86, Cs= 1.65), and aggregate stability(6.02) were significantly improved. The A. fruticosa soil and iron tailings admixture spray sowing restoration model,which effectively improved particle distribution(Cu-= 8.51, Cs= 1.07), increased the number of large aggregates considerably(9.6%), thereby increasing aggregate stability(6.2). The six-hole brick model significantly increased the number of large aggregates(4.0%) and improved the stability of aggregates(6.2). H. rhamnoides direct restoration improved the stability of aggregates(5.1)but showed no other significant improvements. The effect of S. vulgaris direct restoration on matrix structure of iron tailings was not significant. Due to its dependence on matrix structure of iron tailings, the erosion resistance of R.typhina soil spray sowing restoration model was the greatest, while that of S. vulgaris direct restoration was the weakest. There was no significant difference in the erosion resistance of the other models. Overall, vegetation restoration supplemented by soil spray sowing restoration and engineering measures is superior to in situ direct vegetation restoration in the short-term. In-situ direct restoration has long-term ecological significance because of its advanced concept of near-natural restoration and the advantages of low cost, easy operation, and low secondary damage.
基金This work is supported in part by the Natural Science Foundation of China under grants(61503112,61673152 and 61503116).
文摘Recently,the effectiveness of neural networks,especially convolutional neural networks,has been validated in the field of natural language processing,in which,sentiment classification for online reviews is an important and challenging task.Existing convolutional neural networks extract important features of sentences without local features or the feature sequence.Thus,these models do not perform well,especially for transition sentences.To this end,we propose a Piecewise Pooling Convolutional Neural Network(PPCNN)for sentiment classification.Firstly,with a sentence presented by word vectors,convolution operation is introduced to obtain the convolution feature map vectors.Secondly,these vectors are segmented according to the positions of transition words in sentences.Thirdly,the most significant feature of each local segment is extracted using max pooling mechanism,and then the different aspects of features can be extracted.Specifically,the relative sequence of these features is preserved.Finally,after processed by the dropout algorithm,the softmax classifier is trained for sentiment classification.Experimental results show that the proposed method PPCNN is effective and superior to other baseline methods,especially for datasets with transition sentences.
文摘Considering current technology limitation in manufacturing present pin fin heat sinks, a new fabric heat sink has been previously designed. However, there is a lack of an understanding of the heat transferring performance of this new kind of heat sink. Nowadays, finite element analysis has been generally developed for determining heat transfer from in-line and staggered pin fin heat sinks used in electronic packaging applications. In this study, this method is used to predict the heat transfer performance of the new heat sink with woven fabric structure, called fabric pin fin heat sink. Effect of the fin length and the material types made of heat sink on the thermal-structure response of the pin fin was investigated under forced convection. The results show that the minimum temperature of heat sink decreases with an increase of pin fin length,but the decreasing amplitude has decreased. Moreover, the heat transfer performance of fabric heat sink made of continuous carbon Fibers/Polymer (PPS) is worse than that of copper and of aluminum.
基金supported by the National Key R&D Program of China(2021YFE0111100)the Science and Technology Partnership Program,the Ministry of Science and Technology of China(KY202201002)+2 种基金the Jiangsu Provincial Department of Science and Technology(BZ2022017)support of China National Textile and Apparel Council(J202002)Jiangsu Advanced Textile Engineering Technology Center(XJFZ/2021/7).
文摘Calotropis gigantea fiber(CGF)is to a type of seed fiber collected from the fruits of the Calotropis gigantea(CG).From a sustainable biomass resource,CGF is a naturally abundant cellulose fiber with good economic value,excellent biocompatibility and biodegradability.CGF has a high hollow structure and is lightweight,which means CGF has received increasing attention in recent years as the starting material for fabrication of different types of functional materials.In this paper,we have reviewed the morphology and characteristics of CGF,including its biological properties,chemical composition,and physical properties.Additionally,we provide an overview of the recent advancements in the extraction process,toughening treatment process,spinning process,and weaving process of CGF,which have garnered significant attention from researchers in the field.The applications of CGF in the fields of environment processing,antibacterial,and smart materials are also summarized.Finally,the challenges and development prospects of CGF are introduced with the ultimate goal of implementing its potential in real-world applications.
基金Supported by The National Natural Science Foundation of China Youth Project(No.8240122558)the National Key Research and Development Program of China(No.2022YFC3600800)the Special Research Fund for Central Universities,Peking Union Medical College(Young Teacher Project)(No.3332023086).
文摘China faces the dual challenge of population aging and rising disability rates,creating an“aging-disability overlap”that places unprecedented pressure on the nation’s healthcare system.This paper analyzes the complex mechanisms underlying the intersection of aging and disability,revealing that chronic and degenerative diseases are primary contributors to disability among older adults,with disability rates significantly increasing with age and comorbidities.Despite China’s implementation of long-term care insurance pilot programs and community-based elderly care services,multiple challenges persist:insufficient financing sustainability,inconsistent assessment standards,regional development imbalances,professional talent shortages,and social-cultural prejudices.In response,this paper proposes a comprehensive strategy framework aligned with“Healthy China 2030”’s goals of strengthening disability prevention and early intervention mechanisms;enhancing long-term care services with diversified security systems;fostering professional talent development in geriatric and rehabilitation medicine;promoting research and application of intelligent assistive technologies;and creating barrierfree communities within an inclusive social environment.Through these coordinated approaches,we aim to improve the quality of life for older adults with disabilities and facilitate dignified and highquality healthy aging.
基金Supported by the National Key Research and Development Program of China(2022YFC3600800)the China Postdoctoral Science Foundation(2024M760259)+1 种基金the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(GZC20240155,GZC20240134)the Beijing Social Science Fund(20SRC029).
文摘Introduction:As China’s population rapidly ages,concerns have emerged about whether increased longevity among older adults is accompanied by improvements in health status.Methods:This study analyzed data from the 2010 and 2020 Chinese censuses to estimate healthy life expectancy(HLE)at age 60 and examined changes in urban-rural disparities and their driving factors.We applied the Sullivan method to estimate gender-and residence-specific HLE,while using the continuous change decomposition method to analyze how mortality and health status contributed to urban-rural differences.Results:Between 2010 and 2020,both the absolute years of HLE and its proportion of total life expectancy increased,with the urban-rural gap narrowing over time.However,the primary driver of urban-rural HLE disparities has shifted from mortality levels to health status,a pattern more pronounced among men.Decomposition analysis further reveals that compared to 2010,the key age groups contributing to urbanrural HLE disparities in 2020 have shifted to older ages,reflecting a transition in health inequalities as the population ages.These findings suggest that while HLE among China’s older population has improved,the nature of health inequalities is evolving.Conclusions:Future public health policies should place greater emphasis on addressing non-fatal health conditions,particularly in rural areas and among the oldest-old,by improving the accessibility and quality of health services to promote healthy aging and reduce urban-rural health disparities.
文摘苋科(Amaranthaceaesensulato)是石竹目(Caryophyllales)第二大科,目前被普遍接受的苋科为其广义概念,含狭义苋科(Amaranthaceae sensu stricto)和藜科(Chenopodiaceae)。然而到目前为止,藜科是否应作为独立的科还存在争议。此外,广义苋科内部各亚科之间的系统关系也尚未厘清。对广义苋科所有13个亚科代表类群进行取样(共59种),基于8个叶绿体序列片段重建其系统发育关系,并结合分子钟估算,对该科及其主要分支的起源与分化时间进行推测。结果表明,广义苋科与狭义苋科都是很好的单系,但藜科并非单系,因此不支持藜科在科级水平的地位,支持广义苋科的观点。除了多节草亚科(Polycnemoideae)之外,其它亚科的系统位置均得到很好的分辨。分子钟估算结果表明,广义苋科于白垩纪晚期约69.9Ma分化出该科的2个主要分支,且该科在白垩纪-古近纪边界附近时期(约66.0Ma)可能发生过快速辐射分化事件。
基金This research was supported by the National Social Science Fund of China(Grant No.20&ZD090)the Second Tibetan Plateau Scientific Expedition and Research Program(Grant No.2019QZKK0405)the Na-tional Natural Science Foundation of China(Grant No.42071249,Grant No.42001199).
文摘With the rapid development of social economy and urban−rural integration,the phenomenon of farmland aban-donment worldwide has proved to be one of the main trends of land use and land cover change(LUCC),and profoundly affected the rural landscape and regional ecological environment.Restricted by the natural environ-ment,economic development and backward agricultural technology,the phenomenon of farmland abandonment is also common in the Qingzang Plateau(QP).Therefore,this paper adopted the spatial autocorrelation method to analyze the spatial pattern of abandonment in the agricultural and pastoral areas of the Qingzang Plateau(APA−QP)in 2017,and the geographically weighted regression(GWR)model to explore the effects of geograph-ical resources,socio-economic development and location conditions on farmland abandonment.This study found that:1)From 2015 to 2017,the abandoned farmland area in the APA−QP was approximately 18.23×10^(4)ha,with an overall abandonment rate of 15.18%.On the whole,it showed the distribution characteristics of“strong in the south and weak in the north,strong in the east and weak in the west”.2)There were positive spatial correlation between both abandoned area and abandonment rate in the APA−QP,showing“concave”and“convex”patterns,respectively,mainly concentrated in the Huang−shui Valley and the Southeast Tibet;while in the western and northern regions,the degree of abandonment was relatively low.3)Farmland abandonment in the APA−QP was mainly driven by the geographical environment changes and farmers’decision−making on farmland utilization.There was significant spatial heterogeneity on farmland abandonment associated with the impact of geographical resources,socio−economics and location conditions.The geographical resource factors had a positive impact on the abandonment,and were strongly constrained by natural geographic conditions such as altitude and slope.The farmland resources in the Qingzang Plateau are limited,but are of strategic significance for the sustainable development of agriculture in the whole Qingzang Plateau.In order to realize the rational distribution of agricul-ture and animal husbandry and the sustainable utilization of farmland resources in the plateau region,land use strategies should be implemented according to regional differences and regional advantages in order to ensure the ecological environment security of Qingzang Plateau.
基金the Natural Science Foundation of Guangdong Province,China(2019A1515011695)the National Natural Science Foundation of China(31500180).
文摘Pseudocerastium is a monotypic genus in Caryophyllaceae endemic to China.The genus has been widely accepted since it was described in 1998,however its phylogenetic position within Caryophyllaceae has never been studied.In the present study,the whole plastid genome and nuclear ribosomal internal transcribed spacer(ITS)sequences of Pseudocerastium stellarioides was obtained through genome skimming,and the phylogenetic position of the species was studied for the first time.Plastid phylogenomic analysis of Caryophyllaceae revealed that Pseudocerastium is clustered within the tribe Alsineae with strong support.Phylogenetic analyses based on an enlarged taxon sampling of Alsineae using five DNA regions(matK,rbcL,rps16 intron,trnL-F and ITS)revealed that P.stellarioides was nested deeply within Cerastium with strong support.Analyses of morphological character evolution suggest that the ancestral states in Alsineae include three styles and a six-lobed capsule at the apex,while both Cerastium and Pseudocerastium have five styles and ten lobes at the apex of the capsule,further supporting their close relationship.The species Pseudocerastium stellarioides is similar to Cerastium wilsonii in morphology,but differs in having villous indumentum on the lower part of the filaments and compressed globose seeds.Therefore,based on the present molecular and morphological evidence,the generic name Pseudocerastium is reduced here as a new synonym of Cerastium and the species P.stellarioides is transferred to Cerastium as C.jiuhuashanense.
文摘Auditing is a kind of practical economical subject, with a strong practical and operational. It is the backbone course of accounting major and plays a crucial role in the professional cultivation. But recently, in stage of our country university education, audition＇ s teaching effectiveness is almost the lowest in all the core curriculum of accounting professional. Students generally reflect that Auditing is difficult to learn and abstract, while the teachers of auditing courses also reflect students have low interest in auditing learning, meanwhile there is less relevant case, fewer choices and other materials. This paper analyzes the problems and limitations of the auditing teaching, proposes measures about the teaching reform of university auditing, and finally makes our own suggestions about the reform measures of our university auditing teaching research.
