CELL DIVISION CONTROL PROTEIN48(CDC48)is essential for membrane fusion,protein degradation,and other cellular processes.Here,we revealed the crucial role of CDC48B in regulating periclinal cell division in roots by an...CELL DIVISION CONTROL PROTEIN48(CDC48)is essential for membrane fusion,protein degradation,and other cellular processes.Here,we revealed the crucial role of CDC48B in regulating periclinal cell division in roots by analyzing the recessive gen1 mutant.We identified the GEN1 gene through map-based cloning and verified that GEN1 encodes CDC48B.gen1 showed severely inhibited root growth,increased periclinal cell division in the endodermis,defective middle cortex(MC)formation,and altered ground tissue patterning in roots.Consistent with these phenotypes,CYCLIND 6;1(CYCD6;1),a periclinal cell division marker,was upregulated in gen1 compared to Col-0.The ratio of SHR_(pro):SHR-GFP fluorescence in pre-dividing nuclei versus the adjacent stele decreased by 33%in gen1,indicating that the trafficking of SHORT-ROOT(SHR)decreased in gen1 when endodermal cells started to divide.These findings suggest that the loss of function of CDC48B inhibits the intercellular trafficking of SHR from the stele to the endodermis,thereby decreasing SHR accumulation in the endodermis.These findings shed light on the crucial role of CDC48B in regulating periclinal cell division in roots.展开更多
基金supported by the National Natural Science Foundation of China (31570291, 31570246)Funds of Shandong “Double Tops” Program (YL2017YSTD03)+3 种基金Shandong “Foreign Experts Double Hundred” Program (WST2017008)Shandong Key Basic Research (ZR2018ZC08N1)Natural Science Foundation of Heilongjiang Province (C2016002)the Fundamental Research Funds for the Central Universities (2572019CT03).
文摘CELL DIVISION CONTROL PROTEIN48(CDC48)is essential for membrane fusion,protein degradation,and other cellular processes.Here,we revealed the crucial role of CDC48B in regulating periclinal cell division in roots by analyzing the recessive gen1 mutant.We identified the GEN1 gene through map-based cloning and verified that GEN1 encodes CDC48B.gen1 showed severely inhibited root growth,increased periclinal cell division in the endodermis,defective middle cortex(MC)formation,and altered ground tissue patterning in roots.Consistent with these phenotypes,CYCLIND 6;1(CYCD6;1),a periclinal cell division marker,was upregulated in gen1 compared to Col-0.The ratio of SHR_(pro):SHR-GFP fluorescence in pre-dividing nuclei versus the adjacent stele decreased by 33%in gen1,indicating that the trafficking of SHORT-ROOT(SHR)decreased in gen1 when endodermal cells started to divide.These findings suggest that the loss of function of CDC48B inhibits the intercellular trafficking of SHR from the stele to the endodermis,thereby decreasing SHR accumulation in the endodermis.These findings shed light on the crucial role of CDC48B in regulating periclinal cell division in roots.
