Objective:To observe the efficacy and safety of TCM syndrome differentiation-guided herbal intervention for patients with five constitutions during the high-risk window period of acute exacerbation of chronic obstruct...Objective:To observe the efficacy and safety of TCM syndrome differentiation-guided herbal intervention for patients with five constitutions during the high-risk window period of acute exacerbation of chronic obstructive pulmonary disease(AECOPD)based on TCM constitution theory.Methods:A total of 300 AECOPD patients in the high-risk window period(54-66 cases for each constitution)were randomly divided into two groups(150 cases each).The control group received fluticasone furoate/umeclidinium/vilanterol inhalation therapy,while the experimental group was additionally given constitution-specific TCM decoctions(e.g.,Erchen Decoction combined with Sanzi Yangqin Decoction for Phlegm-Dampness constitution).The treatment course was 8 weeks with a 6-month follow-up.CAT score,TCM syndrome score,pulmonary function,6-minute walking distance(6MWD),and levels of CRP and IL-6 were observed.Recurrence and safety indicators were recorded.Results:After treatment,all indicators improved significantly in both groups(p<0.05),with the experimental group showing superior improvements in CAT score,TCM syndrome score,FEV1,6MWD,and inflammatory indicators(p<0.01).The recurrence rate was lower in the experimental group during follow-up(p<0.05).No severe adverse reactions or abnormalities in liver/kidney function were observed in either group.Conclusion:TCM syndrome differentiation treatment guided by constitution theory can improve symptoms,quality of life,and pulmonary function,reduce inflammatory levels and recurrence rate in AECOPD patients during the high-risk window period,with good safety.展开更多
Existing Chinese named entity recognition(NER)research utilises 1D lexicon-based sequence labelling frameworks,which can only recognise flat entities.While lexicons serve as prior knowledge and enhance semantic inform...Existing Chinese named entity recognition(NER)research utilises 1D lexicon-based sequence labelling frameworks,which can only recognise flat entities.While lexicons serve as prior knowledge and enhance semantic information,they also pose completeness and resource requirements limitations.This paper proposes a template-based classification(TC)model to avoid lexicon issues and to identify nested entities.Template-based classification provides a template word for each entity type,which utilises contrastive learning to integrate the common characteristics among entities with the same category.Contrastive learning makes template words the centre points of their category in the vector space,thus improving generalisation ability.Additionally,TC presents a 2D tablefilling label scheme that classifies entities based on the attention distribution of template words.The proposed novel decoder algorithm enables TC recognition of both flat and nested entities simultaneously.Experimental results show that TC achieves the state-ofthe-art performance on five Chinese datasets.展开更多
Age-related macular degeneration(AMD)is a disease that affects the vision of elderly individuals worldwide.Although current therapeutics have shown effectiveness against AMD,some patients may remain unresponsive and c...Age-related macular degeneration(AMD)is a disease that affects the vision of elderly individuals worldwide.Although current therapeutics have shown effectiveness against AMD,some patients may remain unresponsive and continue to experience disease progression.Therefore,in-depth knowledge of the mechanism underlying AMD pathogenesis is urgently required to identify potential drug targets for AMD treatment.Recently,studies have suggested that dysfunction of mitochondria can lead to the aggregation of reactive oxygen species(ROS)and activation of the cyclic GMP-AMP synthase(cGAS)/stimulator of interferon genes(STING)innate immunity pathways,ultimately resulting in sterile inflammation and cell death in various cells,such as cardiomyocytes and macrophages.Therefore,combining strategies targeting mitochondrial dysfunction and inflammatory mediators may hold great potential in facilitating AMD management.Notably,emerging evidence indicates that natural products targeting mitochondrial quality control(MQC)and the cGAS/STING innate immunity pathways exhibit promise in treating AMD.Here,we summarize phytochemicals that could directly or indirectly influence the MQC and the cGAS/STING innate immunity pathways,as well as their interconnected mediators,which have the potential to mitigate oxidative stress and suppress excessive inflammatory responses,thereby hoping to offer new insights into therapeutic interventions for AMD treatment.展开更多
Glioma is the most malignant brain cancer.The neurons,macrophages,T cells and other immune ellls constitute the glioma immunosuppressive microenvironment.The accurate spatial distri-bution of these cells in the glioma...Glioma is the most malignant brain cancer.The neurons,macrophages,T cells and other immune ellls constitute the glioma immunosuppressive microenvironment.The accurate spatial distri-bution of these cells in the glioma microenvironment and its relationship with glioma metastasis is unknown.We constructed a mouse gliomna cell line stably expressing the large Stokes shifted yellow fluorescent protein and applied it to the multicolor immunofluorescence imaging.The inaging data revealed that the neurons were sparsely distributed in the glioma core and the mumber of neurons decreased by 90%compared with normal brain site.The spatial distribution of monocyte-macrophages and microglia is heterogeneous.The monocyte macrophages and T cells were heavily recrnuited into the glioma core and metastasis.There was no significant difference in the distribution of microglia amnong glioma core,margin,and normal brain site.Our results provided new perspectives for targeting immune regulation cells and developing new immuno-therapy strategies for glioma.展开更多
This study presents stability analyses of layered soil slopes in unsaturated conditions and uses a limit equilibrium method to determine the factor of safety involving suction stress of unsaturated soil.One-dimensiona...This study presents stability analyses of layered soil slopes in unsaturated conditions and uses a limit equilibrium method to determine the factor of safety involving suction stress of unsaturated soil.One-dimensional steady infiltration and evaporation conditions are considered in the stability analyses.An example of a two-layered slope in clay and silt is selected to verify the used method by comparing with the results of other methods.Parametric analyses are conducted to explore the influences of the matric suction on the stability of layered soil slopes.The obtained results show that larger suction stress provided in unsaturated clay dominates the stability of the layered slopes.Therefore,the location and thickness of the clay layer have significant influences on slope stability.As the water level decreases,the factor of safety reduces and then increases gradually in most cases.Infiltration/evaporation can obviously affect the stability of unsaturated layered slopes,but their influences depend on the soil property and thickness of the lower soil layer.展开更多
Supramolecular-polymeric hybrid hydrogels combining advantages of the assembled supramolecular networks and polymeric networks have attracted considerable attention recently.However,they are rarely used as multi-funct...Supramolecular-polymeric hybrid hydrogels combining advantages of the assembled supramolecular networks and polymeric networks have attracted considerable attention recently.However,they are rarely used as multi-functional wound dressing materials.In this work,we report a supramolecular-polymeric hybrid hydrogel consisting of a co-assembled DBS-COOH/Naproxen network(DBS/NAP)and a polymeric gelatin(G)/quaternary ammonium group(Q)and phenylboronic acid groups(PBA)-grafted chitosan(QCS-PBA)network(G/QCS)for accelerating skin wound healing.The polymeric networks could obviously enhance the mechanical properties of the drug-derived supramolecular networks.Q groups endowed the hydrogel with enhanced antibacterial properties.Borate ester crosslinking between PBA groups and diol groups in DBS-COOH could effectively scavenge reactive radicals.The hybrid hydrogel exhibited tunable mechanical and adhesive properties due to the temperature-responsive gelatin networks.NAP could gradually release from hybrid hydrogel networks thus continuously relieving inflammation.Finally,the biocompatible,adhesive,antibacterial and antioxidant hybrid G/QCS-DBS/NAP gel could promote the in vivo healing stages in a full-thickness wounds with increased collagen deposition,upregulated CD31 expression and down-regulated TNF-αexpression.Overall,this multi-functional supramolecular-polymeric hybrid hydrogel exhibited great potential for treating skin defects as promising wound dressing materials.展开更多
基金Longquan Yi District Health Bureau Project(Project No.:WJKY2023009)。
文摘Objective:To observe the efficacy and safety of TCM syndrome differentiation-guided herbal intervention for patients with five constitutions during the high-risk window period of acute exacerbation of chronic obstructive pulmonary disease(AECOPD)based on TCM constitution theory.Methods:A total of 300 AECOPD patients in the high-risk window period(54-66 cases for each constitution)were randomly divided into two groups(150 cases each).The control group received fluticasone furoate/umeclidinium/vilanterol inhalation therapy,while the experimental group was additionally given constitution-specific TCM decoctions(e.g.,Erchen Decoction combined with Sanzi Yangqin Decoction for Phlegm-Dampness constitution).The treatment course was 8 weeks with a 6-month follow-up.CAT score,TCM syndrome score,pulmonary function,6-minute walking distance(6MWD),and levels of CRP and IL-6 were observed.Recurrence and safety indicators were recorded.Results:After treatment,all indicators improved significantly in both groups(p<0.05),with the experimental group showing superior improvements in CAT score,TCM syndrome score,FEV1,6MWD,and inflammatory indicators(p<0.01).The recurrence rate was lower in the experimental group during follow-up(p<0.05).No severe adverse reactions or abnormalities in liver/kidney function were observed in either group.Conclusion:TCM syndrome differentiation treatment guided by constitution theory can improve symptoms,quality of life,and pulmonary function,reduce inflammatory levels and recurrence rate in AECOPD patients during the high-risk window period,with good safety.
基金Sichuan Provincial Science and Technology Support Program,Grant/Award Number:2023YFG0151National Natural Science Foundation of China,Grant/Award Numbers:U22B2061,U2336204。
文摘Existing Chinese named entity recognition(NER)research utilises 1D lexicon-based sequence labelling frameworks,which can only recognise flat entities.While lexicons serve as prior knowledge and enhance semantic information,they also pose completeness and resource requirements limitations.This paper proposes a template-based classification(TC)model to avoid lexicon issues and to identify nested entities.Template-based classification provides a template word for each entity type,which utilises contrastive learning to integrate the common characteristics among entities with the same category.Contrastive learning makes template words the centre points of their category in the vector space,thus improving generalisation ability.Additionally,TC presents a 2D tablefilling label scheme that classifies entities based on the attention distribution of template words.The proposed novel decoder algorithm enables TC recognition of both flat and nested entities simultaneously.Experimental results show that TC achieves the state-ofthe-art performance on five Chinese datasets.
基金funded by Chinese NSFC(Grant Nos.:82373336,82303238,and U22A20311,Sichuan Science and Technology Department,China(GrantNos.:2024NSFSC1945,,and 2023NSFSC0667)the Third People's Hospital of Chengdu Clinical Research Program,China(Grant Nos.:CSY-YN-01-2023-013,CSYYN-01-2023-005,and CSY-YN-03-2024-003)+1 种基金Sichuan University“From O to 1”Innovative Research Project,China(Project No.:2023SCUH0024)Health Commission of Chengdu,China(Grant No.:2024291).
文摘Age-related macular degeneration(AMD)is a disease that affects the vision of elderly individuals worldwide.Although current therapeutics have shown effectiveness against AMD,some patients may remain unresponsive and continue to experience disease progression.Therefore,in-depth knowledge of the mechanism underlying AMD pathogenesis is urgently required to identify potential drug targets for AMD treatment.Recently,studies have suggested that dysfunction of mitochondria can lead to the aggregation of reactive oxygen species(ROS)and activation of the cyclic GMP-AMP synthase(cGAS)/stimulator of interferon genes(STING)innate immunity pathways,ultimately resulting in sterile inflammation and cell death in various cells,such as cardiomyocytes and macrophages.Therefore,combining strategies targeting mitochondrial dysfunction and inflammatory mediators may hold great potential in facilitating AMD management.Notably,emerging evidence indicates that natural products targeting mitochondrial quality control(MQC)and the cGAS/STING innate immunity pathways exhibit promise in treating AMD.Here,we summarize phytochemicals that could directly or indirectly influence the MQC and the cGAS/STING innate immunity pathways,as well as their interconnected mediators,which have the potential to mitigate oxidative stress and suppress excessive inflammatory responses,thereby hoping to offer new insights into therapeutic interventions for AMD treatment.
基金supported by the National Natural Science Foundation of China (31700808)the Fundamental Research Funds for the Central Universities (2020kfyXJJS112).
文摘Glioma is the most malignant brain cancer.The neurons,macrophages,T cells and other immune ellls constitute the glioma immunosuppressive microenvironment.The accurate spatial distri-bution of these cells in the glioma microenvironment and its relationship with glioma metastasis is unknown.We constructed a mouse gliomna cell line stably expressing the large Stokes shifted yellow fluorescent protein and applied it to the multicolor immunofluorescence imaging.The inaging data revealed that the neurons were sparsely distributed in the glioma core and the mumber of neurons decreased by 90%compared with normal brain site.The spatial distribution of monocyte-macrophages and microglia is heterogeneous.The monocyte macrophages and T cells were heavily recrnuited into the glioma core and metastasis.There was no significant difference in the distribution of microglia amnong glioma core,margin,and normal brain site.Our results provided new perspectives for targeting immune regulation cells and developing new immuno-therapy strategies for glioma.
基金the National Natural Science Foundation of China(Grant Nos.52078185 and 51878248).
文摘This study presents stability analyses of layered soil slopes in unsaturated conditions and uses a limit equilibrium method to determine the factor of safety involving suction stress of unsaturated soil.One-dimensional steady infiltration and evaporation conditions are considered in the stability analyses.An example of a two-layered slope in clay and silt is selected to verify the used method by comparing with the results of other methods.Parametric analyses are conducted to explore the influences of the matric suction on the stability of layered soil slopes.The obtained results show that larger suction stress provided in unsaturated clay dominates the stability of the layered slopes.Therefore,the location and thickness of the clay layer have significant influences on slope stability.As the water level decreases,the factor of safety reduces and then increases gradually in most cases.Infiltration/evaporation can obviously affect the stability of unsaturated layered slopes,but their influences depend on the soil property and thickness of the lower soil layer.
基金This work was financially supported by the following foundations:National Key Research and Development Program(No.2022YFC2403201)National Science Fund for Distinguished Young Scholars(No.52125305)+2 种基金National Natural Science Foundation of China(No.51903149)the Foundation of Henan Educational Committee(Nos.21A150029,23B430001)the Key Science and Technology Project of Henan Province(Nos.212102310856,222102310595).
文摘Supramolecular-polymeric hybrid hydrogels combining advantages of the assembled supramolecular networks and polymeric networks have attracted considerable attention recently.However,they are rarely used as multi-functional wound dressing materials.In this work,we report a supramolecular-polymeric hybrid hydrogel consisting of a co-assembled DBS-COOH/Naproxen network(DBS/NAP)and a polymeric gelatin(G)/quaternary ammonium group(Q)and phenylboronic acid groups(PBA)-grafted chitosan(QCS-PBA)network(G/QCS)for accelerating skin wound healing.The polymeric networks could obviously enhance the mechanical properties of the drug-derived supramolecular networks.Q groups endowed the hydrogel with enhanced antibacterial properties.Borate ester crosslinking between PBA groups and diol groups in DBS-COOH could effectively scavenge reactive radicals.The hybrid hydrogel exhibited tunable mechanical and adhesive properties due to the temperature-responsive gelatin networks.NAP could gradually release from hybrid hydrogel networks thus continuously relieving inflammation.Finally,the biocompatible,adhesive,antibacterial and antioxidant hybrid G/QCS-DBS/NAP gel could promote the in vivo healing stages in a full-thickness wounds with increased collagen deposition,upregulated CD31 expression and down-regulated TNF-αexpression.Overall,this multi-functional supramolecular-polymeric hybrid hydrogel exhibited great potential for treating skin defects as promising wound dressing materials.
