Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR...Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al.展开更多
The cold plasma(CP)technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon(PAH)in this investigation.Two different CP treatments methods were implemented in the production of beef patti...The cold plasma(CP)technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon(PAH)in this investigation.Two different CP treatments methods were implemented in the production of beef patties,to investigate their inhibition and degradation capacity on PAHs.With 5 different cooking oils and fats addition,the inhibition mechanism of in-package cold plasma(ICP)pretreatment was explored from the aspect of raw patties fatty acids composition variation.The results of principal component analysis showed that the first two principal components accounted for more than 80%of the total variation in the original data,indicating that the content of saturated fatty acids was significantly positively correlated with the formation of PAHs.ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids,which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased.Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls,utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality.In conclusion,CP treatment degraded PAHs through stepwise ring-opening oxidation in 2 reported pathways,the toxicity of PAHs contaminated products was alleviated after CP treatment.展开更多
To the Editor:Systemic lupus erythematosus(SLE)is an autoimmune disease that tends to affect females of childbearing age.There are nearly 4 million people in China currently suffering from SLE,which can cause multiple...To the Editor:Systemic lupus erythematosus(SLE)is an autoimmune disease that tends to affect females of childbearing age.There are nearly 4 million people in China currently suffering from SLE,which can cause multiple organ damage in its early stages and seriously affect patients’quality of life.[1-3]Menstrual disorders are common in SLE patients and vary from heavy menstrual bleeding to amenorrhea.The etiology of menstrual irregularities includes the disease activity itself,glucocorticoids(GCs)treatment,and the use of some immunosuppressants.[4]This multicenter,cross-sectional,web-based observational study investigated menstrual irregularity,pregnancy outcomes,and birth outcomes on SLE patients across China.The research was approved by the Ethics Committee of Peking University People’s Hospital,China(No.2019PHB253-01).The patients were informed that consent was implied with completion of the questionnaire,and the process was anonymous and voluntary.展开更多
基金supported by grants from the Ministry of Agriculture of China (2016ZX08010001)the National Natural Science Foundation of China (31771740)
文摘Rapid and efficient isolation of unknown flanking DNA sequences adjacent to known regions is important for molecular biology research.For this purpose,several PCR-based methods have been reported,including inverse PCR(Uchiyama and Watanabe,2006),ligation-mediated PCR(Yan et al.,2003;Ballester et al.,2005;Wang et al.,2007;Trinh et al.,2012)and randomly primed PCR(Liu and Whittier,1995;Liu et al.,1995;Antal et al.
基金supported by the Key Scientific and Technological Research Projects of Xinjiang Production and Construction Corps (2022AB001)the Henan Key Laboratory of Cold Chain Food Quality and Safety Control (CCFQ2022)+2 种基金the National Key R&D Program of China (2019YFC1606200),funded by Ministry of Science and Technology of the People’s Republic of Chinathe China Agriculture Research System (CARS-41), which was funded by the Chinese Ministry of Agriculturethe Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)
文摘The cold plasma(CP)technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon(PAH)in this investigation.Two different CP treatments methods were implemented in the production of beef patties,to investigate their inhibition and degradation capacity on PAHs.With 5 different cooking oils and fats addition,the inhibition mechanism of in-package cold plasma(ICP)pretreatment was explored from the aspect of raw patties fatty acids composition variation.The results of principal component analysis showed that the first two principal components accounted for more than 80%of the total variation in the original data,indicating that the content of saturated fatty acids was significantly positively correlated with the formation of PAHs.ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids,which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased.Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls,utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality.In conclusion,CP treatment degraded PAHs through stepwise ring-opening oxidation in 2 reported pathways,the toxicity of PAHs contaminated products was alleviated after CP treatment.
基金Beijing Natural Science Foundation(No.7192211)China International Medical Foundation(No.Z-2018-40-2101)
文摘To the Editor:Systemic lupus erythematosus(SLE)is an autoimmune disease that tends to affect females of childbearing age.There are nearly 4 million people in China currently suffering from SLE,which can cause multiple organ damage in its early stages and seriously affect patients’quality of life.[1-3]Menstrual disorders are common in SLE patients and vary from heavy menstrual bleeding to amenorrhea.The etiology of menstrual irregularities includes the disease activity itself,glucocorticoids(GCs)treatment,and the use of some immunosuppressants.[4]This multicenter,cross-sectional,web-based observational study investigated menstrual irregularity,pregnancy outcomes,and birth outcomes on SLE patients across China.The research was approved by the Ethics Committee of Peking University People’s Hospital,China(No.2019PHB253-01).The patients were informed that consent was implied with completion of the questionnaire,and the process was anonymous and voluntary.
