Objectives:Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia and Philadelphia-like B-cell acute lymphoblastic leukemia(Ph+/Ph-like ALL)constitute the majority of relapsed/refractory B-ALL(R/R B-ALL)...Objectives:Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia and Philadelphia-like B-cell acute lymphoblastic leukemia(Ph+/Ph-like ALL)constitute the majority of relapsed/refractory B-ALL(R/R B-ALL)cases,highlighting an urgent need to discover new therapeutic targets.This study aims to elucidate the mechanisms underlying poor prognosis in Ph+/Ph-like ALL through transcriptome sequencing and functional cytological assays,with the goal of informing new clinical treatment strategies.Results:Transcriptomic analysis of Ph+/Ph-like ALL patients revealed that low expression of P2X Purinoceptor 1(P2RX1)was associated with unfavorable outcomes.Specifically,patients with poor prognosis and low P2RX1 expression exhibited downregulation of genes involved in energy and calcium metabolism pathways,along with upregulation of genes governing key cellular processes such as cell proliferation(e.g.,MYC),cell cycle progression(e.g.,CCND2),and apoptosis inhibition(e.g.,DASP6).Cellular experiments demonstrated that SUP-B15 cells overexpressing P2RX1 displayed elevated intracellular levels of ATP,calcium,and glucose,together with enhanced glycolytic capacity,compared to empty vector controls.Treatment of SUP-B15 cells with dexamethasone(Dex),Imatinib,or their combination significantly suppressed proliferation and promoted apoptosis,which was accompanied by increases in intracellular ATP,calcium,and glucose.Moreover,exogenous ATP administration(a P2RX1 agonist)enhanced apoptosis and inhibited proliferation in control cells.Conversely,treatment with NF449(a P2RX1 inhibitor)increased proliferation in both P2RX1-overexpressing and control SUP-B15 cells.Conclusion:Our findings indicate that P2RX1 may exert this function through modulating energy metabolism and calcium homeostasis,resulting in elevated intracellular calcium levels.Sustained elevation of calcium promotes apoptosis,whereas exogenous ATP activates P2RX1,enhances calcium influx,and attenuates the suppression of apoptosis associated with P2RX1 underexpression,ultimately correlating with improved treatment response.展开更多
The scientific assessment of ecosystem ser-vice value(ESV)plays a critical role in regional ecologi-cal protection and management,rational land use planning,and the establishment of ecological security barriers.The ec...The scientific assessment of ecosystem ser-vice value(ESV)plays a critical role in regional ecologi-cal protection and management,rational land use planning,and the establishment of ecological security barriers.The ecosystem service value of the Northeast Forest Belt from 2005 to 2020 was assessed,focusing on spatial–temporal changes and the driving forces behind these dynamics.Using multi-source data,the equivalent factor method,and geo-graphic detectors,we analyzed natural and socio-economic factors affecting the region.which was crucial for effective ecological conservation and land-use planning.Enhanced the effectiveness of policy formulation and land use plan-ning.The results show that the ESV of the Northeast Forest Belt exhibits an overall increasing trend from 2005 to 2020,with forests and wetlands contributing the most.However,there are significant differences between forest belts.Driven by natural and socio-economic factors,the ESV of forest belts in Heilongjiang and Jilin provinces showed significant growth.In contrast,the ESV of Forest Belts in Liaoning and Inner Mongolia of China remains relatively stable,but the spatial differentiation within these regions is characterized by significant clustering of high-value and low-value areas.Furthermore,climate regulation and hydrological regulation services were identified as the most important ecological functions in the Northeast Forest Belt,contributing greatly to regional ecological stability and human well-being.The ESV in the Northeast Forest Belt is improved during the study period,but the stability of the ecosystem is still chal-lenged by the dual impacts of natural and socio-economic factors.To further optimize regional land use planning and ecological protection policies,it is recommended to prior-itize the conservation of high-ESV areas,enhance ecological restoration efforts for wetlands and forests,and reasonably control the spatial layout of urban expansion and agricul-tural development.Additionally,this study highlights the importance of tailored ecological compensation policies and strategic land-use planning to balance environmental protec-tion and economic growth.展开更多
Objective:To investigate the efficacy and adverse reactions of volumetric modulated arc therapy(VMAT)radiotherapy combined with raltitrexed chemotherapy in the treatment of elderly patients with esophageal cancer.Meth...Objective:To investigate the efficacy and adverse reactions of volumetric modulated arc therapy(VMAT)radiotherapy combined with raltitrexed chemotherapy in the treatment of elderly patients with esophageal cancer.Methods:A total of 86 elderly patients with esophageal cancer admitted to our hospital between February 2024 and February 2025 were enrolled in this study and equally divided into two groups:a control group receiving VMAT radiotherapy alone and a study group receiving VMAT radiotherapy combined with raltitrexed chemotherapy,with 43 patients in each group,to compare the therapeutic outcomes between the two treatment approaches.Results:The study group demonstrated significantly higher objective remission and disease control rates than the control group(P<0.05).Post-treatment levels of HSP90α,Cyfra21-1,and CEA were markedly reduced in the study group relative to the control group(P<0.05).Adverse reaction incidence showed no notable difference between the two groups(P>0.05).Quality of life(QOL)scores were significantly elevated in the study group compared to the control group at both 3-and 6-month follow-ups(P<0.05).Conclusion:VMAT radiotherapy combined with raltitrexed chemotherapy can improve the short-term efficacy,reduce tumor marker levels,and improve the quality of life of elderly patients with esophageal cancer.The treatment has fewer adverse reactions and better patient tolerance.展开更多
Objective:To analyze the efficacy of whole-course local simultaneous integrated boost intensity-modulated radiotherapy(SIB-IMRT)on patients with locally advanced esophageal squamous cell carcinoma(ESCC).Methods:88 pat...Objective:To analyze the efficacy of whole-course local simultaneous integrated boost intensity-modulated radiotherapy(SIB-IMRT)on patients with locally advanced esophageal squamous cell carcinoma(ESCC).Methods:88 patients with ESCC admitted to the hospital between October 2022 and October 2024 were selected and randomly divided into two groups using a random number table.The experimental group received SIB-IMRT treatment,while the control group received conventional intensity-modulated radiotherapy(C-IMRT).The objective remission rate,immune function,tumor markers,and adverse reaction rate were compared between the two groups.Results:The objective remission rate in the experimental group was higher than that in the control group(P<0.05).Before treatment,there was no difference in immune function levels and tumor marker levels between the two groups(P>0.05).After treatment,the immune function levels in the experimental group were better than those in the control group,and the tumor marker levels were lower than those in the control group(P<0.05).The adverse reaction rate in the experimental group was lower than that in the control group(P<0.05).Conclusion:SIB-IMRT can improve the objective remission rate of patients with ESCC,protect their immune function,down-regulate tumor marker levels,and prevent side effects after treatment.展开更多
This study focused to determine the bacteriological quality of different commonly used water sources, including boreholes, hand pumps, surface water, household containers, dug well and cement reservoirs in Pelengana c...This study focused to determine the bacteriological quality of different commonly used water sources, including boreholes, hand pumps, surface water, household containers, dug well and cement reservoirs in Pelengana commune, in Mali with special reference to the internally displaced people camps. Four hundred and eight water samples were collected from different sites and sources, from July 2016 to June 2017. Water samples were examined for total coliform, fecal coliform and fecal enterococci counts using the most probable number method. Also, samples were investigated for the presence of enteric bacteria isolated, namely Escherichia coli, Enterococcus fecalis, Bacillus, Citrobacter and Enterobacter. Results revealed that the three indicators bacteria (Total Coliform (48.7%), Fecal Coliform (100%), and Fecal enterococci (37.01%)) were found above permissible limit for drinking water. The highest number of the indicators bacteria found was 1800 TC/100ml water. Enteric bacteria isolated were Escherichia coli (25.7%), Enterococcus fecalis (21.91%), Bacillus (16.72%), Enterobacter (4.56%) and Citrobacter (3.91%). The most contaminated water source was surface water (15.28%) followed by household containers (13.65%), boreholes (8.75%), cement reservoirs (7.47%), hand pumps (5.31%) and dug wells (2.7%). These findings illustrate that contamination varied with seasons, and the highest level was observed in rainy season (30.02%) compared to the dry season (23.14%). All sources of water in the study sites were contaminated. It is suggested that the outright prevention of surface water consumption should be employed and monitoring of the quality of water resources is needed.展开更多
The 1815 eruption of the Tambora volcano led to the ＂Year without a Summer＂ and caused serious crop failure and famines in 1816 across Europe and North America. However, few reports are available on Tambora＇s influ...The 1815 eruption of the Tambora volcano led to the ＂Year without a Summer＂ and caused serious crop failure and famines in 1816 across Europe and North America. However, few reports are available on Tambora＇s influence in China despite the region＇s susceptibility to monsoonal volcanic perturbation. This study presents a systemic analysis of the climatic and related social responses to the Tambora perturbation in China, by using two independent lines of proxy records and projecting the responses on top of the impacts averaged over all tropical eruptions of the past mil- lennium. Both the tree ring and Chinese documentary proxies show that Tambora induced a cold excursion, which caused severe frost damage, snow and ice accumulations that are uncommonly seen in southern China. Cold temper- ature tends to cause drought by suppressing evaporation and monsoonal circulation- a hydroclimate response that is evident in the tree-ring-based Monsoon Asia Drought Atlas but largely missing in a multiproxy precipitation recon- struction. Historical records of drought, flood, frost, and famine also show fairly mild responses outside southern China, which may be partially due to the insensitivity of documentary records to the Tambora-induced perturbation, or the cold background climate set up by the low solar insolation of the coincident Dalton Minimum and a preceding unknown eruption in 1809. The results presented here provide new insights into the spatial extent and characteristics of the Tambora perturbation, by providing a systematic evaluation of the climatic aftermath in China in parallel to that in Europe and North America. They also argue for the integral use of multiple proxies from different regions of the world to gain a better understanding of the climatic impacts for individual volcanic eruptions.展开更多
The term of interstitial pneumonia with autoimmune features(IPAF),based on a combination of clinical,serological,and morphological domains(specific chest imaging features,histopathological features,or multi-compartmen...The term of interstitial pneumonia with autoimmune features(IPAF),based on a combination of clinical,serological,and morphological domains(specific chest imaging features,histopathological features,or multi-compartment involvement,including non-specific interstitial pneumonia[NSIP],organizing pneumonia,NSIP with organizing pneumonia overlap and lymphoid interstitial pneumonia).展开更多
基金supported by Guangdong Province Basic and Applied Basic Research Fund Project(2023A1515220104)Open Fund of Key Laboratory of Hepatoaplenic Surgery,Ministry of Education(Award Number:GPKF202407).
文摘Objectives:Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia and Philadelphia-like B-cell acute lymphoblastic leukemia(Ph+/Ph-like ALL)constitute the majority of relapsed/refractory B-ALL(R/R B-ALL)cases,highlighting an urgent need to discover new therapeutic targets.This study aims to elucidate the mechanisms underlying poor prognosis in Ph+/Ph-like ALL through transcriptome sequencing and functional cytological assays,with the goal of informing new clinical treatment strategies.Results:Transcriptomic analysis of Ph+/Ph-like ALL patients revealed that low expression of P2X Purinoceptor 1(P2RX1)was associated with unfavorable outcomes.Specifically,patients with poor prognosis and low P2RX1 expression exhibited downregulation of genes involved in energy and calcium metabolism pathways,along with upregulation of genes governing key cellular processes such as cell proliferation(e.g.,MYC),cell cycle progression(e.g.,CCND2),and apoptosis inhibition(e.g.,DASP6).Cellular experiments demonstrated that SUP-B15 cells overexpressing P2RX1 displayed elevated intracellular levels of ATP,calcium,and glucose,together with enhanced glycolytic capacity,compared to empty vector controls.Treatment of SUP-B15 cells with dexamethasone(Dex),Imatinib,or their combination significantly suppressed proliferation and promoted apoptosis,which was accompanied by increases in intracellular ATP,calcium,and glucose.Moreover,exogenous ATP administration(a P2RX1 agonist)enhanced apoptosis and inhibited proliferation in control cells.Conversely,treatment with NF449(a P2RX1 inhibitor)increased proliferation in both P2RX1-overexpressing and control SUP-B15 cells.Conclusion:Our findings indicate that P2RX1 may exert this function through modulating energy metabolism and calcium homeostasis,resulting in elevated intracellular calcium levels.Sustained elevation of calcium promotes apoptosis,whereas exogenous ATP activates P2RX1,enhances calcium influx,and attenuates the suppression of apoptosis associated with P2RX1 underexpression,ultimately correlating with improved treatment response.
基金funded by the Central University D Project(HFW230600022)National Natural Science Foundation of China(71973021)+1 种基金National Natural Science Foundation Youth Funding Project(72003022)Heilongjiang Province University Think Tank Open Topic(ZKKF2022173).
文摘The scientific assessment of ecosystem ser-vice value(ESV)plays a critical role in regional ecologi-cal protection and management,rational land use planning,and the establishment of ecological security barriers.The ecosystem service value of the Northeast Forest Belt from 2005 to 2020 was assessed,focusing on spatial–temporal changes and the driving forces behind these dynamics.Using multi-source data,the equivalent factor method,and geo-graphic detectors,we analyzed natural and socio-economic factors affecting the region.which was crucial for effective ecological conservation and land-use planning.Enhanced the effectiveness of policy formulation and land use plan-ning.The results show that the ESV of the Northeast Forest Belt exhibits an overall increasing trend from 2005 to 2020,with forests and wetlands contributing the most.However,there are significant differences between forest belts.Driven by natural and socio-economic factors,the ESV of forest belts in Heilongjiang and Jilin provinces showed significant growth.In contrast,the ESV of Forest Belts in Liaoning and Inner Mongolia of China remains relatively stable,but the spatial differentiation within these regions is characterized by significant clustering of high-value and low-value areas.Furthermore,climate regulation and hydrological regulation services were identified as the most important ecological functions in the Northeast Forest Belt,contributing greatly to regional ecological stability and human well-being.The ESV in the Northeast Forest Belt is improved during the study period,but the stability of the ecosystem is still chal-lenged by the dual impacts of natural and socio-economic factors.To further optimize regional land use planning and ecological protection policies,it is recommended to prior-itize the conservation of high-ESV areas,enhance ecological restoration efforts for wetlands and forests,and reasonably control the spatial layout of urban expansion and agricul-tural development.Additionally,this study highlights the importance of tailored ecological compensation policies and strategic land-use planning to balance environmental protec-tion and economic growth.
文摘Objective:To investigate the efficacy and adverse reactions of volumetric modulated arc therapy(VMAT)radiotherapy combined with raltitrexed chemotherapy in the treatment of elderly patients with esophageal cancer.Methods:A total of 86 elderly patients with esophageal cancer admitted to our hospital between February 2024 and February 2025 were enrolled in this study and equally divided into two groups:a control group receiving VMAT radiotherapy alone and a study group receiving VMAT radiotherapy combined with raltitrexed chemotherapy,with 43 patients in each group,to compare the therapeutic outcomes between the two treatment approaches.Results:The study group demonstrated significantly higher objective remission and disease control rates than the control group(P<0.05).Post-treatment levels of HSP90α,Cyfra21-1,and CEA were markedly reduced in the study group relative to the control group(P<0.05).Adverse reaction incidence showed no notable difference between the two groups(P>0.05).Quality of life(QOL)scores were significantly elevated in the study group compared to the control group at both 3-and 6-month follow-ups(P<0.05).Conclusion:VMAT radiotherapy combined with raltitrexed chemotherapy can improve the short-term efficacy,reduce tumor marker levels,and improve the quality of life of elderly patients with esophageal cancer.The treatment has fewer adverse reactions and better patient tolerance.
文摘Objective:To analyze the efficacy of whole-course local simultaneous integrated boost intensity-modulated radiotherapy(SIB-IMRT)on patients with locally advanced esophageal squamous cell carcinoma(ESCC).Methods:88 patients with ESCC admitted to the hospital between October 2022 and October 2024 were selected and randomly divided into two groups using a random number table.The experimental group received SIB-IMRT treatment,while the control group received conventional intensity-modulated radiotherapy(C-IMRT).The objective remission rate,immune function,tumor markers,and adverse reaction rate were compared between the two groups.Results:The objective remission rate in the experimental group was higher than that in the control group(P<0.05).Before treatment,there was no difference in immune function levels and tumor marker levels between the two groups(P>0.05).After treatment,the immune function levels in the experimental group were better than those in the control group,and the tumor marker levels were lower than those in the control group(P<0.05).The adverse reaction rate in the experimental group was lower than that in the control group(P<0.05).Conclusion:SIB-IMRT can improve the objective remission rate of patients with ESCC,protect their immune function,down-regulate tumor marker levels,and prevent side effects after treatment.
文摘This study focused to determine the bacteriological quality of different commonly used water sources, including boreholes, hand pumps, surface water, household containers, dug well and cement reservoirs in Pelengana commune, in Mali with special reference to the internally displaced people camps. Four hundred and eight water samples were collected from different sites and sources, from July 2016 to June 2017. Water samples were examined for total coliform, fecal coliform and fecal enterococci counts using the most probable number method. Also, samples were investigated for the presence of enteric bacteria isolated, namely Escherichia coli, Enterococcus fecalis, Bacillus, Citrobacter and Enterobacter. Results revealed that the three indicators bacteria (Total Coliform (48.7%), Fecal Coliform (100%), and Fecal enterococci (37.01%)) were found above permissible limit for drinking water. The highest number of the indicators bacteria found was 1800 TC/100ml water. Enteric bacteria isolated were Escherichia coli (25.7%), Enterococcus fecalis (21.91%), Bacillus (16.72%), Enterobacter (4.56%) and Citrobacter (3.91%). The most contaminated water source was surface water (15.28%) followed by household containers (13.65%), boreholes (8.75%), cement reservoirs (7.47%), hand pumps (5.31%) and dug wells (2.7%). These findings illustrate that contamination varied with seasons, and the highest level was observed in rainy season (30.02%) compared to the dry season (23.14%). All sources of water in the study sites were contaminated. It is suggested that the outright prevention of surface water consumption should be employed and monitoring of the quality of water resources is needed.
基金Supported by the National Natural Science Foundation of China(41501066)National(Key) Basic Research and Development(973) Program of China(2015CB953601)
文摘The 1815 eruption of the Tambora volcano led to the ＂Year without a Summer＂ and caused serious crop failure and famines in 1816 across Europe and North America. However, few reports are available on Tambora＇s influence in China despite the region＇s susceptibility to monsoonal volcanic perturbation. This study presents a systemic analysis of the climatic and related social responses to the Tambora perturbation in China, by using two independent lines of proxy records and projecting the responses on top of the impacts averaged over all tropical eruptions of the past mil- lennium. Both the tree ring and Chinese documentary proxies show that Tambora induced a cold excursion, which caused severe frost damage, snow and ice accumulations that are uncommonly seen in southern China. Cold temper- ature tends to cause drought by suppressing evaporation and monsoonal circulation- a hydroclimate response that is evident in the tree-ring-based Monsoon Asia Drought Atlas but largely missing in a multiproxy precipitation recon- struction. Historical records of drought, flood, frost, and famine also show fairly mild responses outside southern China, which may be partially due to the insensitivity of documentary records to the Tambora-induced perturbation, or the cold background climate set up by the low solar insolation of the coincident Dalton Minimum and a preceding unknown eruption in 1809. The results presented here provide new insights into the spatial extent and characteristics of the Tambora perturbation, by providing a systematic evaluation of the climatic aftermath in China in parallel to that in Europe and North America. They also argue for the integral use of multiple proxies from different regions of the world to gain a better understanding of the climatic impacts for individual volcanic eruptions.
基金This study was supported by the National Natural Science Foundation of China(No.82170076)the Jiangsu Provincial Medical Talent(No.ZDRCA2016058)。
文摘The term of interstitial pneumonia with autoimmune features(IPAF),based on a combination of clinical,serological,and morphological domains(specific chest imaging features,histopathological features,or multi-compartment involvement,including non-specific interstitial pneumonia[NSIP],organizing pneumonia,NSIP with organizing pneumonia overlap and lymphoid interstitial pneumonia).
