Cinnamomum japonicum Sieb.is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value.In this study,we successfully developed a large-scale micropropagation protocol for C....Cinnamomum japonicum Sieb.is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value.In this study,we successfully developed a large-scale micropropagation protocol for C.japonicum for the first time.Sterilized shoots were excised and used as explants for shoot induction on several basal media,supplemented with different concentrations of plant growth regulators(PGRs),such as Thidiazuron(TDZ),N^(6)-Benzyladenine(6-benzylaminopurine)(BA),α-naphthaleneacetic acid(NAA)and Gibberellic acid(GA_(3)).After comparison,the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog(MS)medium containing 0.5 mg L^(-1)BA,0.05 mg L^(-1)NAA and 0.2 mg L^(-1)GA_(3),which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d,respectively.Then,elongated adventitious shoots were transferred to induce roots.86.7%of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^(-1)NAA and 0.1 mg L^(-1)BA.The earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 d.Our study shows that C.japonicum can be successfully regenerated through de novo organogenesis,which lays a foundation for future transformation research on this tree.展开更多
Cerasus Campanulata is one of several species belonging to the Prunoideae focke,a subfamily of the flowering plant Rosaceae.We investigated the details of its chloroplast genome which may reveal its genus independent ...Cerasus Campanulata is one of several species belonging to the Prunoideae focke,a subfamily of the flowering plant Rosaceae.We investigated the details of its chloroplast genome which may reveal its genus independent of morphological determination.Here,we determined the complete chloroplast(cp)genome sequence of C.campanulata and performed sequence analysis to reveal the presence of 18 forward repeats,20 palindrome repeats,2 complement repeats,4 reverse repeats and 93 simple sequence repeats(SSRs).We additionally performed a comparative study of C.campanulata and seven other Prunoideae focke species.Then,maximum parsimony(MP)and maximum likelihood(ML)phylogenetic analyses were carried out in the little part of Rosaceae,respectively.The results strongly support a position of C.campanulata as a member of the Cerasus in the Rosaceae family.Moreover,the complete cp genome can be used for plant phylogenetic and evolutionary studies that will provide insight into the degree of gene conservation.展开更多
基金This research is supported by Key research and development plan of Jiangsu Province(BE2017376)Foundation of Jiangsu forestry bureau(LYKJ[2017]42)the Qinglan project of Jiangsu province and Priority Academic Program Development of Jiangsu Higher Education Institutions to J.H.Chen,and the Nature Science Foundation of China(31770715)to T.L.Cheng.
文摘Cinnamomum japonicum Sieb.is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value.In this study,we successfully developed a large-scale micropropagation protocol for C.japonicum for the first time.Sterilized shoots were excised and used as explants for shoot induction on several basal media,supplemented with different concentrations of plant growth regulators(PGRs),such as Thidiazuron(TDZ),N^(6)-Benzyladenine(6-benzylaminopurine)(BA),α-naphthaleneacetic acid(NAA)and Gibberellic acid(GA_(3)).After comparison,the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog(MS)medium containing 0.5 mg L^(-1)BA,0.05 mg L^(-1)NAA and 0.2 mg L^(-1)GA_(3),which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d,respectively.Then,elongated adventitious shoots were transferred to induce roots.86.7%of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^(-1)NAA and 0.1 mg L^(-1)BA.The earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 d.Our study shows that C.japonicum can be successfully regenerated through de novo organogenesis,which lays a foundation for future transformation research on this tree.
基金This work was supported by key research and development plan of Foundation of Jiangsu Forestry Bureau(LYKJ[2017]42),Jiangsu Province(BE2017376)the Nature Science Foundation of China(31770715)the Qinglan project of Jiangsu Province,and Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘Cerasus Campanulata is one of several species belonging to the Prunoideae focke,a subfamily of the flowering plant Rosaceae.We investigated the details of its chloroplast genome which may reveal its genus independent of morphological determination.Here,we determined the complete chloroplast(cp)genome sequence of C.campanulata and performed sequence analysis to reveal the presence of 18 forward repeats,20 palindrome repeats,2 complement repeats,4 reverse repeats and 93 simple sequence repeats(SSRs).We additionally performed a comparative study of C.campanulata and seven other Prunoideae focke species.Then,maximum parsimony(MP)and maximum likelihood(ML)phylogenetic analyses were carried out in the little part of Rosaceae,respectively.The results strongly support a position of C.campanulata as a member of the Cerasus in the Rosaceae family.Moreover,the complete cp genome can be used for plant phylogenetic and evolutionary studies that will provide insight into the degree of gene conservation.
