With the widespread application of Staggered Counter-rotating Rotor(SCR)systems in eVTOL and UAV configurations,a comprehensive understanding of SCR performance under Outof-Ground Effect(OGE)and In-Ground Effect(IGE)c...With the widespread application of Staggered Counter-rotating Rotor(SCR)systems in eVTOL and UAV configurations,a comprehensive understanding of SCR performance under Outof-Ground Effect(OGE)and In-Ground Effect(IGE)conditions is crucial for aircraft design and landing safety.This study experimentally measured the changes in thrust and torque of the upper and lower rotors in an SCR system under varying axial and radial distances.It focuses on the interaction mechanisms between the upper and lower rotors and conducts specific IGE state experiments for certain SCR configurations.The findings reveal that changes in the lower rotor predominantly influence the overall performance of the SCR system,regardless of OGE or IGE conditions.Under OGE conditions,radial distance has a more significant impact than axial distance.Conversely,under IGE conditions,the axial distance plays a critical role in improving SCR system performance.These results provide a broad parameter range to assess SCR system performance variations,offering guidance for the design of new concept rotorcraft configurations and the development of aerodynamic prediction models under IGE conditions.展开更多
Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and monitoring.Herein,we prop...Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and monitoring.Herein,we propose a sensitive method by designing a gold nanoparticle(AuNP)probe for visually intracellular detection of telomerase activity.The AuNPs were functionalized with a telomer-ase substrate primer(SH-prime).A 6-carboxy-fluorescein(FAM)modified strand(FAM-probe)was attached to the surface of AuNP through its complementary stand(SH-attach).In the absence of telomerase,the fluorescence resonance energy transfer(FRET)from FAM to AuNPs results in efficient fluorescence quenching.In the presence of telomerase,SH-primers on AuNPs were extended with the repeat units(TTAGGGG)n.The extension sequence triggered the strand displacement of FAM-probe to restore the fluorescence signals.It is worth mentioning that the proposed strategy does not need to design complex hairpin structure and allows the meas-urement of telomerase in crude cell extracts down to 0.5 HeLa cells/μL in 2 h.In addition,the present sensing platform can be ap-plied to the visually intracellular detection of telomerase activity in living cells.展开更多
基金funded by the National Natural Science Foundation of China(Nos.52202443,52275114)the China Postdoctoral Science Foundation(No.2023M731656)+3 种基金the National Key Laboratory of Helicopter Aeromechanics Foundation,China(No.2023-HA-LB-067-05e)the Natural Science Foundation of Jiangsu Province,China(No.BK20220898)the Jiangsu Funding Program for Excellent Postdoctoral Talent,China(No.JB0202003)the Aeronautical Science Foundation of China(No.20232010052002)。
文摘With the widespread application of Staggered Counter-rotating Rotor(SCR)systems in eVTOL and UAV configurations,a comprehensive understanding of SCR performance under Outof-Ground Effect(OGE)and In-Ground Effect(IGE)conditions is crucial for aircraft design and landing safety.This study experimentally measured the changes in thrust and torque of the upper and lower rotors in an SCR system under varying axial and radial distances.It focuses on the interaction mechanisms between the upper and lower rotors and conducts specific IGE state experiments for certain SCR configurations.The findings reveal that changes in the lower rotor predominantly influence the overall performance of the SCR system,regardless of OGE or IGE conditions.Under OGE conditions,radial distance has a more significant impact than axial distance.Conversely,under IGE conditions,the axial distance plays a critical role in improving SCR system performance.These results provide a broad parameter range to assess SCR system performance variations,offering guidance for the design of new concept rotorcraft configurations and the development of aerodynamic prediction models under IGE conditions.
基金supported by the National Natural Science Foundation of China(Grant No.22037004)。
文摘Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and monitoring.Herein,we propose a sensitive method by designing a gold nanoparticle(AuNP)probe for visually intracellular detection of telomerase activity.The AuNPs were functionalized with a telomer-ase substrate primer(SH-prime).A 6-carboxy-fluorescein(FAM)modified strand(FAM-probe)was attached to the surface of AuNP through its complementary stand(SH-attach).In the absence of telomerase,the fluorescence resonance energy transfer(FRET)from FAM to AuNPs results in efficient fluorescence quenching.In the presence of telomerase,SH-primers on AuNPs were extended with the repeat units(TTAGGGG)n.The extension sequence triggered the strand displacement of FAM-probe to restore the fluorescence signals.It is worth mentioning that the proposed strategy does not need to design complex hairpin structure and allows the meas-urement of telomerase in crude cell extracts down to 0.5 HeLa cells/μL in 2 h.In addition,the present sensing platform can be ap-plied to the visually intracellular detection of telomerase activity in living cells.
