Rice(Oryza sativa L.)is essential for global food security,but faces increasing threats from extreme weather.High temperatures during the grain-filling stage lower rice yields and quality(Wada et al.,2019).Studies hav...Rice(Oryza sativa L.)is essential for global food security,but faces increasing threats from extreme weather.High temperatures during the grain-filling stage lower rice yields and quality(Wada et al.,2019).Studies have revealed the mechanisms by which heat stress impairs rice quality during the grain-filling stage,including the involvement of factors such as DG1(Qin et al.,2021b),Oscp Hsp70-2(Tabassum et al.,2020),and Osb ZIP58Β(Xu et al.,2020).展开更多
Continuously increasing global temperatures present great challenges to food security.Grain size,one of the critical components determining grain yield in rice(Oryza sativa L.),is a prime target for genetic breeding.T...Continuously increasing global temperatures present great challenges to food security.Grain size,one of the critical components determining grain yield in rice(Oryza sativa L.),is a prime target for genetic breeding.Thus,there is an immediate need for genetic improvement in rice to maintain grain yield under heat stress.However,quantitative trait loci(QTLs)endowing heat stress tolerance and grain size in rice are extremely rare.Here,we identified a novel negative regulator with pleiotropic effects,Thermo‐Tolerance and grain Length 1(TTL1),from the super pan‐genomic and transcriptomic data.Loss‐of‐function mutations in TTL1 enhanced heat tolerance,and caused an increase in grain size by coordinating cell expansion and proliferation.TTL1 was shown to function as a transcriptional regulator and localized to the nucleus and cell membrane.Furthermore,haplotype analysis showed that hapL and hapS of TTL1 were obviously correlated with variations of thermotolerance and grain size in a core collection of cultivars.Genome evolution analysis of available rice germplasms suggested that TTL1 was selected during domestication of the indica and japonica rice subspecies,but still had much breeding potential for increasing grain length and thermotolerance.These findings provide insights into TTL1 as a novel potential target for the development of high‐yield and thermotolerant rice varieties.展开更多
基金supported by the National Natural Science Foundation of China(32270250 and 32400202)National Science Foundation of Fujian Province of China(2022J02004)+1 种基金Fujian Provincial Science and Technology Key Project(2024NZ029027)China Postdoctoral Science Foundation(2022M712665)。
文摘Rice(Oryza sativa L.)is essential for global food security,but faces increasing threats from extreme weather.High temperatures during the grain-filling stage lower rice yields and quality(Wada et al.,2019).Studies have revealed the mechanisms by which heat stress impairs rice quality during the grain-filling stage,including the involvement of factors such as DG1(Qin et al.,2021b),Oscp Hsp70-2(Tabassum et al.,2020),and Osb ZIP58Β(Xu et al.,2020).
基金This work was supported by the National Natural Science Foundation of Fujian Province(2022J01470)the Youth Innovation of Chinese Academy of Agricultural Sciences(Y20230C36)+1 种基金the Special Project for Public Welfare Research Institute of Fujian Province(2021R1027005)the Freely Exploring Technological Innovation Projects of Fujian Academy of Agricultural Sciences(ZYTS202216).
文摘Continuously increasing global temperatures present great challenges to food security.Grain size,one of the critical components determining grain yield in rice(Oryza sativa L.),is a prime target for genetic breeding.Thus,there is an immediate need for genetic improvement in rice to maintain grain yield under heat stress.However,quantitative trait loci(QTLs)endowing heat stress tolerance and grain size in rice are extremely rare.Here,we identified a novel negative regulator with pleiotropic effects,Thermo‐Tolerance and grain Length 1(TTL1),from the super pan‐genomic and transcriptomic data.Loss‐of‐function mutations in TTL1 enhanced heat tolerance,and caused an increase in grain size by coordinating cell expansion and proliferation.TTL1 was shown to function as a transcriptional regulator and localized to the nucleus and cell membrane.Furthermore,haplotype analysis showed that hapL and hapS of TTL1 were obviously correlated with variations of thermotolerance and grain size in a core collection of cultivars.Genome evolution analysis of available rice germplasms suggested that TTL1 was selected during domestication of the indica and japonica rice subspecies,but still had much breeding potential for increasing grain length and thermotolerance.These findings provide insights into TTL1 as a novel potential target for the development of high‐yield and thermotolerant rice varieties.
