Multicellular three-dimensional(3D)bioprinting technology,a pivotal in vitro approach in tissue engineering and disease modeling,enables the co-culture of multiple cell populations within 3D architectures while preser...Multicellular three-dimensional(3D)bioprinting technology,a pivotal in vitro approach in tissue engineering and disease modeling,enables the co-culture of multiple cell populations within 3D architectures while preserving physiological interactions(1).This strategy facilitates the precise simulation of human histogenesis and pathogenesis through four principal modalities:organoid-based systems that recapitulate tissue self-organization,air-liquid interface(ALI)platforms facilitating epithelial-mesenchymal crosstalk analysis,3D microfluidic devices for spatiotemporal control of biomolecular gradients,and bioprinting techniques achieving micron-level spatial patterning of heterogeneous cells(2).Each modality addresses distinct experimental demands in reconstructing multicellular microenvironments,collectively enhancing the fidelity of drug screening and advancing mechanobiological research.展开更多
基金supported by the National Natural Science Foundation of China(No.32271470).
文摘Multicellular three-dimensional(3D)bioprinting technology,a pivotal in vitro approach in tissue engineering and disease modeling,enables the co-culture of multiple cell populations within 3D architectures while preserving physiological interactions(1).This strategy facilitates the precise simulation of human histogenesis and pathogenesis through four principal modalities:organoid-based systems that recapitulate tissue self-organization,air-liquid interface(ALI)platforms facilitating epithelial-mesenchymal crosstalk analysis,3D microfluidic devices for spatiotemporal control of biomolecular gradients,and bioprinting techniques achieving micron-level spatial patterning of heterogeneous cells(2).Each modality addresses distinct experimental demands in reconstructing multicellular microenvironments,collectively enhancing the fidelity of drug screening and advancing mechanobiological research.
