The dysfunction of the lysosome and autophagy-lysosome system serves as a driving force for neurodegenerative diseases,metabolic disorders,inflammatory conditions,and other related diseases,closely influencing their o...The dysfunction of the lysosome and autophagy-lysosome system serves as a driving force for neurodegenerative diseases,metabolic disorders,inflammatory conditions,and other related diseases,closely influencing their onset and progression.Therefore,restoring the function of the lysosome or autophagy-lysosome system has become an increasingly crucial therapeutic strategy in disease management.In this review,we will introduce the lysosomal biogenesis,structure,and function,as well as the biological process of the autophagy-lysosome system.Various diseases closely associated with lysosomal/autophagic dysfunction are also reviewed,emphasizing the significance of targeting the function of the lysosome or autophagy-lysosome system in disease treatment.Finally,we focus on engineered nanomaterials that have the capabilities to restore the function of the lysosome or autophagy-lysosome system,and summarize different strategies and methods for achieving this goal.This review aims to elucidate the latest progress in the field of nanomedicine for lysosomal/autophagic defect-related diseases and inspire the development of innovative and clinically valuable nanomedicines.展开更多
Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examine...Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe_(3)O_(4) particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.展开更多
Morphological parameters of coarse aggregates,including the angularity index (Al),flat and elongated ratio,flatness ratio,and sphericity,strongly affect the performance of asphalt concrete.A device must be developed t...Morphological parameters of coarse aggregates,including the angularity index (Al),flat and elongated ratio,flatness ratio,and sphericity,strongly affect the performance of asphalt concrete.A device must be developed to efficiently and accurately measure these parameters.Herein,a charge-coupled device camera and a linear profile scanner are combined to evaluate the parameters.The Al is calculated using three methods.The results show that the fitted-ellipse method provides superior results among the three Al measurement methods.Moreover,the use of a linear profile scanner improves the Al measurement accuracy.Therefore,the proposed combination has high measurement efficiency and is suitable for batch inspections.展开更多
Dilated cardiomyopathy(DCM)is the leading cause of heart transplantation.By microRNA(miRNA)array,a Kaposi’s sarcoma-associated herpes virus(KSHV)-encoded miRNA,kshv-miR-K12-1-5p,was detected in patients with DCM.The ...Dilated cardiomyopathy(DCM)is the leading cause of heart transplantation.By microRNA(miRNA)array,a Kaposi’s sarcoma-associated herpes virus(KSHV)-encoded miRNA,kshv-miR-K12-1-5p,was detected in patients with DCM.The KSHV DNA load and kshv-miR-K12-1-5p level in plasma from 696 patients with DCM were measured and these patients were followed-up.展开更多
Electrocatalytic ammonia oxidation reaction(eAOR)is of significance to ammonia fuel economy and the production of valuable N-containing products,such as nitrite,nitrate and hydrazine.The study of well-defined molecula...Electrocatalytic ammonia oxidation reaction(eAOR)is of significance to ammonia fuel economy and the production of valuable N-containing products,such as nitrite,nitrate and hydrazine.The study of well-defined molecular catalysts offers rich insights in terms of the detailed mechanism of ammonia oxidation.This review analyzes the thermodynamics of ammonia oxidation reactions and summarizes the current progress in molecular electrocatalysts in this booming field.We emphasized the factors that influence the selectivity of products and further discussed the challenges in designing efficient catalysts.展开更多
基金supported by the Taishan Scholars Program of Shandong Province(No.tsqn202103112)the Shandong Provincial Natural Science Foundation(No.ZR2021QB202)+2 种基金the Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(No.2021KJ052)the Shandong-Chongqing Science and Technology Cooperation Project for Technological Innovation and Application Development(No.CSTB2023TIAD-LDX0015)the Supporting Fund for Leading Talents above Provincial Level in Yantai,China.
文摘The dysfunction of the lysosome and autophagy-lysosome system serves as a driving force for neurodegenerative diseases,metabolic disorders,inflammatory conditions,and other related diseases,closely influencing their onset and progression.Therefore,restoring the function of the lysosome or autophagy-lysosome system has become an increasingly crucial therapeutic strategy in disease management.In this review,we will introduce the lysosomal biogenesis,structure,and function,as well as the biological process of the autophagy-lysosome system.Various diseases closely associated with lysosomal/autophagic dysfunction are also reviewed,emphasizing the significance of targeting the function of the lysosome or autophagy-lysosome system in disease treatment.Finally,we focus on engineered nanomaterials that have the capabilities to restore the function of the lysosome or autophagy-lysosome system,and summarize different strategies and methods for achieving this goal.This review aims to elucidate the latest progress in the field of nanomedicine for lysosomal/autophagic defect-related diseases and inspire the development of innovative and clinically valuable nanomedicines.
基金This study was supported by the Beijing Municipal Science&Technology Commission(No.Z07000200540704)the China Postdoctoral Science Foundation(No.20090450417)and the Tsinghua Yuyuan Medical Fund.
文摘Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe_(3)O_(4) particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.
文摘Morphological parameters of coarse aggregates,including the angularity index (Al),flat and elongated ratio,flatness ratio,and sphericity,strongly affect the performance of asphalt concrete.A device must be developed to efficiently and accurately measure these parameters.Herein,a charge-coupled device camera and a linear profile scanner are combined to evaluate the parameters.The Al is calculated using three methods.The results show that the fitted-ellipse method provides superior results among the three Al measurement methods.Moreover,the use of a linear profile scanner improves the Al measurement accuracy.Therefore,the proposed combination has high measurement efficiency and is suitable for batch inspections.
基金This work was supported by grants from the National Natural Science Foundation of China(nos.81822002,82270363,91839302,81630010,81790624,31771264,31800973,and 82000387)National Key R&D Program of China(no.2017YFC0909400)the Natural Science Foundation of Hubei Province(no.2020CFA016).The funders had no role in study design,data collection and analysis,manuscript preparation,or decision to publish.
文摘Dilated cardiomyopathy(DCM)is the leading cause of heart transplantation.By microRNA(miRNA)array,a Kaposi’s sarcoma-associated herpes virus(KSHV)-encoded miRNA,kshv-miR-K12-1-5p,was detected in patients with DCM.The KSHV DNA load and kshv-miR-K12-1-5p level in plasma from 696 patients with DCM were measured and these patients were followed-up.
基金supported by the National Key R&D Program of China(2022YFC3401802)the National Natural Science Foundation of China(22279105)+4 种基金the Zhejiang Provincial Natural Science Foundation(XHD24B0201)the Starting-up Package from Westlake Universitythe Kunpeng Research Fund from Zhejiang ProvinceResearch Center for Industries of the FutureZhejiang Baima Lake Laboratory。
文摘Electrocatalytic ammonia oxidation reaction(eAOR)is of significance to ammonia fuel economy and the production of valuable N-containing products,such as nitrite,nitrate and hydrazine.The study of well-defined molecular catalysts offers rich insights in terms of the detailed mechanism of ammonia oxidation.This review analyzes the thermodynamics of ammonia oxidation reactions and summarizes the current progress in molecular electrocatalysts in this booming field.We emphasized the factors that influence the selectivity of products and further discussed the challenges in designing efficient catalysts.
