AIM: To investigate the designation, synthesis and biological activity of against vascular endothelial growth factor165 (VEGF165) ribozyme.METHODS: The ribozyme against VEGF165 was designed with computer. The transcri...AIM: To investigate the designation, synthesis and biological activity of against vascular endothelial growth factor165 (VEGF165) ribozyme.METHODS: The ribozyme against VEGF165 was designed with computer. The transcriptional vector was constructed which included the anti-VEGF165 ribozyme and 5', 3' selfsplicing ribozymes. The hammerhead ribozyme and substrate VEGF165 mRNA were synthesized through transcription in vitro.The cleavage activity of the ribozyme on target RNA was observed in a cell-free system.PESULTS: The anti-VEGF165 ribozyme was released properly from the transcription of pGEMRz212 cleaved by 5' and 3' self-splicing ribozymes which retained its catalytic activity,and the cleavage efficiency of ribozyme reached 90.7%.CONCLUSION: The anti-VEGF165 ribozyme designed with computer can cleave VEGF165 mRNA effectively.展开更多
2D van der Waals(vdW)magnets have opened intriguing prospects for next-generation spintronic nanodevices.Machine learning techniques and density functional theory calculations enable the discovery of 2D vdW magnets to...2D van der Waals(vdW)magnets have opened intriguing prospects for next-generation spintronic nanodevices.Machine learning techniques and density functional theory calculations enable the discovery of 2D vdW magnets to be accelerated;however,current computational frameworks based on these state-ofthe-art approaches cannot offer probability analysis on whether a 2D vdW magnet can be experimentally demonstrated.Herein,a new framework can be established to overcome this challenge.Via the framework,2D vdW magnets with high probability for experimental demonstration are captured from materials science literature.The key to the successful establishment is the introduction of the theory of mutual information.Historical validation of predictions substantiates the high reliability of the framework.For example,half of the 302D vdW magnets discovered in the literature published prior to 2017 have been experimentally demonstrated in the subsequent years.This framework has the potential to become a revolutionary force for progressing experimental discovery of 2D vdW magnets.展开更多
文摘AIM: To investigate the designation, synthesis and biological activity of against vascular endothelial growth factor165 (VEGF165) ribozyme.METHODS: The ribozyme against VEGF165 was designed with computer. The transcriptional vector was constructed which included the anti-VEGF165 ribozyme and 5', 3' selfsplicing ribozymes. The hammerhead ribozyme and substrate VEGF165 mRNA were synthesized through transcription in vitro.The cleavage activity of the ribozyme on target RNA was observed in a cell-free system.PESULTS: The anti-VEGF165 ribozyme was released properly from the transcription of pGEMRz212 cleaved by 5' and 3' self-splicing ribozymes which retained its catalytic activity,and the cleavage efficiency of ribozyme reached 90.7%.CONCLUSION: The anti-VEGF165 ribozyme designed with computer can cleave VEGF165 mRNA effectively.
基金supported by the National Natural Science Foundation of China(21825103,22103026,and U21A2069)the Fundamental Research Funds for the Central Universities(2020kfyXJJS050).
文摘2D van der Waals(vdW)magnets have opened intriguing prospects for next-generation spintronic nanodevices.Machine learning techniques and density functional theory calculations enable the discovery of 2D vdW magnets to be accelerated;however,current computational frameworks based on these state-ofthe-art approaches cannot offer probability analysis on whether a 2D vdW magnet can be experimentally demonstrated.Herein,a new framework can be established to overcome this challenge.Via the framework,2D vdW magnets with high probability for experimental demonstration are captured from materials science literature.The key to the successful establishment is the introduction of the theory of mutual information.Historical validation of predictions substantiates the high reliability of the framework.For example,half of the 302D vdW magnets discovered in the literature published prior to 2017 have been experimentally demonstrated in the subsequent years.This framework has the potential to become a revolutionary force for progressing experimental discovery of 2D vdW magnets.
