Dear Editor,This letter is concerned with the problem of time-varying formation tracking for heterogeneous multi-agent systems(MASs) under directed switching networks. For this purpose, our first step is to present so...Dear Editor,This letter is concerned with the problem of time-varying formation tracking for heterogeneous multi-agent systems(MASs) under directed switching networks. For this purpose, our first step is to present some sufficient conditions for the exponential stability of a particular category of switched systems.展开更多
Abuse of amphetamine-based stimulants is a primary public health concern.Recent studies have underscored a troubling escalation in the inappropriate use of prescription amphetamine-based stimulants.However,the neuroph...Abuse of amphetamine-based stimulants is a primary public health concern.Recent studies have underscored a troubling escalation in the inappropriate use of prescription amphetamine-based stimulants.However,the neurophysiological mechanisms underlying the impact of acute methamphetamine exposure(AME)on sleep homeostasis remain to be explored.This study employed non-human primates and electroencephalogram(EEG)sleep staging to evaluate the influence of AME on neural oscillations.The primary focus was on alterations in spindles,delta oscillations,and slow oscillations(SOs)and their interactions as conduits through which AME influences sleep stability.AME predominantly diminishes sleep-spindle waves in the non-rapid eye movement 2(NREM2)stage,and impacts SOs and delta waves differentially.Furthermore,the competitive relationships between SO/delta waves nesting with sleep spindles were selectively strengthened by methamphetamine.Complexity analysis also revealed that the SO-nested spindles had lost their ability to maintain sleep depth and stability.In summary,this finding could be one of the intrinsic electrophysiological mechanisms by which AME disrupted sleep homeostasis.展开更多
Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs ...Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.展开更多
Biological nanotechnologies based on functional nanoplatforms have synergistically catalyzed the emergence of cancer therapies.As a subtype of metal-organic frameworks(MOFs),zeolitic imidazolate frameworks(ZIFs)have e...Biological nanotechnologies based on functional nanoplatforms have synergistically catalyzed the emergence of cancer therapies.As a subtype of metal-organic frameworks(MOFs),zeolitic imidazolate frameworks(ZIFs)have exploded in popularity in the field of biomaterials as excellent protective materials with the advantages of conformational flexibility,thermal and chemical stability,and functional controllability.With these superior properties,the applications of ZIF-based materials in combination with various therapies for cancer treatment have grown rapidly in recent years,showing remarkable achievements and great potential.This review elucidates the recent advancements in the use of ZIFs as drug delivery agents for cancer therapy.The structures,synthesis methods,properties,and various modifiers of ZIFs used in oncotherapy are presented.Recent advances in the application of ZIF-based nanoparticles as single or combination tumor treatments are reviewed.Furthermore,the future prospects,potential limitations,and challenges of the application of ZIF-based nanomaterials in cancer treatment are discussed.We except to fully explore the potential of ZIF-based materials to present a clear outline for their application as an effective cancer treatment to help them achieve early clinical application.展开更多
In modern industrial design trends featuring with integration,miniaturization,and versatility,there is a growing demand on the utilization of microstructural array devices.The measurement of such microstructural array...In modern industrial design trends featuring with integration,miniaturization,and versatility,there is a growing demand on the utilization of microstructural array devices.The measurement of such microstructural array components often encounters challenges due to the reduced scale and complex structures,either by contact or noncontact optical approaches.Among these microstructural arrays,there are still no optical measurement methods for micro corner-cube reflector arrays.To solve this problem,this study introduces a method for effectively eliminating coherent noise and achieving surface profile reconstruction in interference measurements of microstructural arrays.The proposed denoising method allows the calibration and inverse solving of system errors in the frequency domain by employing standard components with known surface types.This enables the effective compensation of the complex amplitude of non-sample coherent light within the interferometer optical path.The proposed surface reconstruction method enables the profile calculation within the situation that there is complex multi-reflection during the propagation of rays in microstructural arrays.Based on the measurement results,two novel metrics are defined to estimate diffraction errors at array junctions and comprehensive errors across multiple array elements,offering insights into other types of microstructure devices.This research not only addresses challenges of the coherent noise and multi-reflection,but also makes a breakthrough for quantitively optical interference measurement of microstructural array devices.展开更多
Harnessing bacteria for superoxide production in bioremediation holds immense promise,yet its practical application is hindered by slow production rates and the relatively weak redox potential of superoxide.This study...Harnessing bacteria for superoxide production in bioremediation holds immense promise,yet its practical application is hindered by slow production rates and the relatively weak redox potential of superoxide.This study delves into a cost-effective approach to amplify superoxide production using an Arthrobacter strain,a prevalent soil bacterial genus.Our research reveals that introducing a carbon source along with specific iron-binding ligands,including deferoxamine(DFO),diethylenetriamine pentaacetate(DTPA),citrate,and oxalate,robustly augments microbial superoxide generation.Moreover,our findings suggest that these iron-binding ligands play a pivotal role in converting superoxide into hydroxyl radicals by modulating the electron transfer rate between Fe(Ⅲ)/Fe(Ⅱ)and superoxide.Remarkably,among the tested ligands,only DTPA emerges as a potent promoter of this conversion process when complexed with Fe(Ⅲ).We identify an optimal Fe(Ⅲ)to DTPA ratio of approximately 1:1 for enhancing hydroxyl radical production within the Arthrobacter culture.This research underscores the efficacy of simultaneously introducing carbon sources and DTPA in facilitating superoxide production and its subsequent conversion to hydroxyl radicals,significantly elevating bioremediation performance.Furthermore,our study reveals that DTPA augments superoxide production in cultures of diverse soils,with various soil microorganisms beyond Arthrobacter identified as contributors to superoxide generation.This emphasizes the universal applicability of DTPA across multiple bacterial genera.In conclusion,our study introduces a promising methodology for enhancing microbial superoxide production and its conversion into hydroxyl radicals.These findings hold substantial implications for the deployment of microbial reactive oxygen species in bioremediation,offering innovative solutions for addressing environmental contamination challenges.展开更多
A critical pathway towards enhancing pulp mill biorefineries is to integrate the extraction and utilization of hemicelluloses into the pulping processes.Hence,an industrial pre-extraction strategy for hemicelluloses t...A critical pathway towards enhancing pulp mill biorefineries is to integrate the extraction and utilization of hemicelluloses into the pulping processes.Hence,an industrial pre-extraction strategy for hemicelluloses targeting eucalyptus kraft pulping process was developed.Alkaline solution or pulping white liquor was used to pre-extract hemicelluloses before the actual pulping process.The response surface methodology(RSM)technique was applied to investigate the most suitable conditions to maximize the yield of these hemicelluloses while simultaneously minimizing the damage to pulp yields and properties.Temperature(105 to 155℃),alkali concentration(3%to 8%),sulfidity(20%to 30%)and retention time(19 to 221 min)were combined to evaluate their effects on hemicellulose yields and chemical structures.The optimal pre-extraction conditions identified in this work(5.75%NaOH concentration,25%sulfidity at 135℃for 60 min)successfully allowed recovering 4.8%of hemicelluloses(based on the wood dry mass)and limited damages to pulp yields and properties.The cellulose content in pulp can even be increased by about 10%.Hemicellulose emulsification properties were also evaluated,which were comparable to synthetic emulsifiers.This study provides an industrial pathway to effectively separate and utilize wood hemicelluloses from the pulping process,which has the potential to improve the economy and material utilization of pulp and paper mills.展开更多
Mesenchymal stem cells(MSCs)have emerged as promising candidates for idiopathic pulmonary fibrosis(IPF)therapy.Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are ...Mesenchymal stem cells(MSCs)have emerged as promising candidates for idiopathic pulmonary fibrosis(IPF)therapy.Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are of great significance for improving the efficacy of MSC-based IPF treatment.Therefore,dual-functional Au-based nanoparticles(Au@PEG@PEI@TAT NPs,AuPPT)were fabricated by sequential modification of cationic polymer polyetherimide(PEI),polyethylene glycol(PEG),and transactivator of transcription(TAT)penetration peptide on AuNPs,to co-deliver retinoic acid(RA)and microRNA(miRNA)for simultaneously enhancing MSC survive and real-time imaging tracking of MSCs during IPF treatment.AuPPT NPs,with good drug loading and cellular uptake abilities,could efficiently deliver miRNA and RA to protect MSCs from reactive oxygen species and reduce their expression of apoptosis executive protein Caspase 3,thus prolonging the survival time of MSC after transplantation.In themeantime,the intracellular accumulation of AuPPT NPs enhanced the computed tomography imaging contrast of transplantedMSCs,allowing them to be visually tracked in vivo.This study establishes an Au-based dual-functional platform for drug delivery and cell imaging tracking,which provides a new strategy for MSC-related IPF therapy.展开更多
Macrophage immunotherapy represents an emerging therapeutic approach aimed at modulating the immune response to alleviate disease symptoms.Nanomaterials(NMs)have been engineered to monitor macrophage metabolism,enabli...Macrophage immunotherapy represents an emerging therapeutic approach aimed at modulating the immune response to alleviate disease symptoms.Nanomaterials(NMs)have been engineered to monitor macrophage metabolism,enabling the evaluation of disease progression and the replication of intricate physiological signal patterns.They achieve this either directly or by delivering regulatory signals,thereby mapping phenotype to effector functions through metabolic repurposing to customize macrophage fate for therapy.However,a comprehensive summary regarding NM-mediated macrophage visualization and coordinated metabolic rewiring to maintain phenotypic equilibrium is currently lacking.This review aims to address this gap by outlining recent advancements in NM-based metabolic immunotherapy.We initially explore the relationship between metabolism,polarization,and disease,before delving into recent NM innovations that visualize macrophage activity to elucidate disease onset and fine-tune its fate through metabolic remodeling for macrophage-centered immunotherapy.Finally,we discuss the prospects and challenges of NM-mediated metabolic immunotherapy,aiming to accelerate clinical translation.We anticipate that this review will serve as a valuable reference for researchers seeking to leverage novel metabolic intervention-matched immunomodulators in macrophages or other fields of immune engineering.展开更多
Following publication of the original article[1],the authors reported an error in the last author’s name,it was mistakenly written as“Jun Den”.The correct author’s name“Jun Deng”has been updated in this Correction.
Accurately predicting the production rate and estimated ultimate recovery(EUR)of shale oil wells is vital for efficient shale oil development.Although numerical simulations provide accurate predictions,their high time...Accurately predicting the production rate and estimated ultimate recovery(EUR)of shale oil wells is vital for efficient shale oil development.Although numerical simulations provide accurate predictions,their high time,data,and labor demands call for a swifter,yet precise,method.This study introduces the DuongeCNNeLSTM(D-C-L)model,which integrates a convolutional neural network(CNN)with a long short-term memory(LSTM)network and is grounded on the empirical Duong model for physical constraints.Compared to traditional approaches,the D-C-L model demonstrates superior precision,efficiency,and cost-effectiveness in predicting shale oil production.展开更多
Background Structural imaging holds great potential for precise targeting and stimulation for deep brain stimulation(DBS).The anatomical information it provides may serve as potential biomarkers for predicting the eff...Background Structural imaging holds great potential for precise targeting and stimulation for deep brain stimulation(DBS).The anatomical information it provides may serve as potential biomarkers for predicting the efficacy of DBS in treatment-resistant depression(TRD).Aims The primary aim is to identify preoperative imaging biomarkers that correlate with the efficacy of DBS in patients with TRD.Methods Preoperative imaging parameters were estimated and correlated with the 6-month clinical outcome of patients with TRD receiving combined bed nucleus of the stria terminalis(BNST)-nucleus accumbens(NAc)DBS.White matter(WM)properties were extracted and compared between the response/non-response and remission/non-remission groups.Structural connectome was constructed and analysed using graph theory.Distances of the volume of activated tissue(VAT)to the main modulating tracts were also estimated to evaluate the correlations.Results Differences in fibre bundle properties of tracts,including superior thalamic radiation and reticulospinal tract,were observed between the remission and nonremission groups.Distance of the centre of the VAT to tracts connecting the ventral tegmental area and the anterior limb of internal capsule on the left side varied between the remission and non-remission groups(p=0.010,t=3.07).The normalised clustering coefficient(γ)and the small-world property(σ)in graph analysis correlated with the symptom improvement after the correction of age.Conclusions Presurgical structural alterations in WM tracts connecting the frontal area with subcortical regions,as well as the distance of the VAT to the modulating tracts,may influence the clinical outcome of BNST-NAc DBS.These findings provide potential imaging biomarkers for the DBS treatment for patients with TRD.展开更多
Quantum technologies rely on creating and manipulating entangled sources,which are essential for quantum information,communication,and imaging.By integrating quantum technologies and all-dielectric metasurfaces,the pe...Quantum technologies rely on creating and manipulating entangled sources,which are essential for quantum information,communication,and imaging.By integrating quantum technologies and all-dielectric metasurfaces,the performance of miniature display devices can be enhanced to a higher level.Miniature display technology,such as virtual reality display,has achieved original commercial success,and was initially applied to immersive games and interactive scenes.While the consumer market has quickly adopted this technology,several areas remain for improvement,including concerns around bulkiness,dual-channel display,and noise reduction.Here,we experimentally realize a quantum meta-hologram concept demonstration of a miniature display.We fabricate an ultracompact meta-hologram based on 1μm thick titanium dioxide(TiO_(2)).The meta-hologram can be remotely switched with heralding technique and is robust against noise with the quantum entangled source.The platform can alter the miniature display channel by manipulating heralding photons’polarization,removing speckles and multiple reflective light noise,improving imaging contrast,and potentially decreasing device weight.Imaging contrast increases from 0.36 dB under speckle noise influences to 6.8 dB in quantum correlation imaging.This approach has the potential to miniaturize quantum displays and quantum communication devices.展开更多
Effective monitoring and management of microbial risk factors in wastewater treatment plants(WWTPs)effluents require a comprehensive investigation of these risks.A global survey on microbial risk factors in WWTP efflu...Effective monitoring and management of microbial risk factors in wastewater treatment plants(WWTPs)effluents require a comprehensive investigation of these risks.A global survey on microbial risk factors in WWTP effluents could reveal important insights into their risk features.This study aims to explore the abundance and types of antibiotic resistance genes(ARGs),virulence factor genes(VFGs),the vector of ARG/VFG,and dominant pathogens in global WWTP effluents.We collected 113 metagenomes of WWTP effluents from the Sequence Read Archive of the National Center for Biotechnology Information and characterized the microbial risk factors.Our results showed that multidrug resistance was the dominant ARG type,while offensive virulence factors were the most abundant type of VFGs.The most dominant types of ARGs in the vector of plasmid and phage were both aminoglycoside resistance,which is concerning as aminoglycosides are often a last resort for treating multi-resistant infections.Acinetobacter baumannii was the most dominant pathogen,rather than Escherichia coli,and a weak negative correlation between Escherichia coli and two other dominant pathogens(Acinetobacter baumannii and Bacteroides uniformis)suggests that using Escherichia coli as a biological indicator for all pathogens in WWTP effluents may not be appropriate.The Getah virus was the most dominant virus found in global WWTP effluents.Our study presents a comprehensive global-scale investigation of microbial risk factors in WWTP effluents,providing valuable insights into the potential risks associated with WWTP effluents and contributing to the monitoring and control of these risks.展开更多
Electrochemical lithium extraction from salt lakes is an effective strategy for obtaining lithium at a low cost.Nevertheless,the elevated Mg:Li ratio and the presence of numerous coexisting ions in salt lake brines gi...Electrochemical lithium extraction from salt lakes is an effective strategy for obtaining lithium at a low cost.Nevertheless,the elevated Mg:Li ratio and the presence of numerous coexisting ions in salt lake brines give rise to challenges,such as prolonged lithium extraction periods,diminished lithium extraction efficiency,and considerable environmental pollution.In this work,Li FePO4(LFP)served as the electrode material for electrochemical lithium extraction.The conductive network in the LFP electrode was optimized by adjusting the type of conductive agent.This approach resulted in high lithium extraction efficiency and extended cycle life.When the single conductive agent of acetylene black(AB)or multiwalled carbon nanotubes(MWCNTs)was replaced with the mixed conductive agent of AB/MWCNTs,the average diffusion coefficient of Li+in the electrode increased from 2.35×10^(-9)or 1.77×10^(-9)to 4.21×10^(-9)cm^(2)·s^(-1).At the current density of 20 mA·g^(-1),the average lithium extraction capacity per gram of LFP electrode increased from 30.36 mg with the single conductive agent(AB)to 35.62 mg with the mixed conductive agent(AB/MWCNTs).When the mixed conductive agent was used,the capacity retention of the electrode after 30 cycles reached 82.9%,which was considerably higher than the capacity retention of 65.8%obtained when the single AB was utilized.Meanwhile,the electrode with mixed conductive agent of AB/MWCNTs provided good cycling performance.When the conductive agent content decreased or the loading capacity increased,the electrode containing the mixed conductive agent continued to show excellent electrochemical performance.Furthermore,a self-designed,highly efficient,continuous lithium extraction device was constructed.The electrode utilizing the AB/MWCNT mixed conductive agent maintained excellent adsorption capacity and cycling performance in this device.This work provides a new perspective for the electrochemical extraction of lithium using LFP electrodes.展开更多
Nickel-rich layered oxide cathode(LiNi_(x)Co_(y)Mn_(1−x−y)O_(2),x>0.5,NCM)shows substantial potential for applications in longer-range electrical vehicles.However,the rapid capacity decay and serious safety concern...Nickel-rich layered oxide cathode(LiNi_(x)Co_(y)Mn_(1−x−y)O_(2),x>0.5,NCM)shows substantial potential for applications in longer-range electrical vehicles.However,the rapid capacity decay and serious safety concerns impede its practical viability.This work provides a hydrogen-bonded organic framework(HOF)modification strategy to simultaneously improve the electrochemical performance,thermal stability and incombustibility of separator.Melamine cyanurate(MCA),as a low-cost and reliable flame-retardant HOF,was implemented in the separator modification layer,which can prevent the battery short circuit even at a high temperature.In addition,the supermolecule properties of MCA provide unique physical and chemical microenvironment for regulating ion-transport behavior in electrolyte.The MCA coating layer enabled the nickel-rich layered oxide cathode with a high-capacity retention of 90.3%after 300 cycles at 1.0 C.Collectively,the usage of MCA in lithium-ion batteries(LIBs)affords a simple,low-cost and efficient strategy to improve the security and service life of nickel-rich layered cathodes.展开更多
While KRAS mutation is the leading cause of low survival rates in lung cancer bone metastasis patients,effective treatments are still lacking.Here,we identified homeobox C10(HOXC10)as a lynchpin in pan-KRAS-mutant lun...While KRAS mutation is the leading cause of low survival rates in lung cancer bone metastasis patients,effective treatments are still lacking.Here,we identified homeobox C10(HOXC10)as a lynchpin in pan-KRAS-mutant lung cancer bone metastasis.展开更多
A numerical control (NC) tool path of digital CAD model is widely generated as a set of short line segments in machining. However, there are three shortcomings in the linear tool path, such as discontinuities of tange...A numerical control (NC) tool path of digital CAD model is widely generated as a set of short line segments in machining. However, there are three shortcomings in the linear tool path, such as discontinuities of tangency and curvature, huge number of line segments, and short lengths of line segments. These disadvantages hinder the development of high speed machining. To smooth the linear tool path and improve machining efficiency of short line segments, this paper presents an optimal feed interpolator based on G^2 continuous Bézier curves for the linear tool path. First, the areas suitable for fitting are screened out based on the geometric characteristics of continuous short segments (CSSs). CSSs in every area are compressed and fitted into a G^2 Continuous Bézier curve by using the least square method. Then a series of cubic Bézier curves are generated. However, the junction between adjacent Bézier curves is only G^0 continuous. By adjusting the control points and inserting Bézier transition curves between adjacent Bézier curves, the G^2 continuous tool path is constructed. The fitting error is estimated by the second-order Taylor formula. Without iteration, the fitting algorithm can be implemented in real-time environment. Second, the optimal feed interpolator considering the comprehensive constraints (such as the chord error constraint, the maximum normal acceleration, servo capacity of each axis, etc.) is proposed. Simulation and experiment are conducted. The results shows that the proposed method can generate smooth path, decrease the amount of segments and reduce machining time for machining of linear tool path. The proposed research provides an effective method for high-speed machining of complex 2-D/3-D profiles described by short line segments.展开更多
Hydrogen-bonded organic frameworks(HOFs)have emerged as a new class of crystalline porous materials,and their application in membrane technology needs to be explored.Herein,for the first time,we demonstrated the utili...Hydrogen-bonded organic frameworks(HOFs)have emerged as a new class of crystalline porous materials,and their application in membrane technology needs to be explored.Herein,for the first time,we demonstrated the utilization of HOF-based mixed-matrix membrane for CO_(2) separation.HOF-21,a unique metallo-hydrogen-bonded organic framework material,was designed and processed into nanofillers via amine modulator,uniformly dispersing with Pebax polymer.Featured with the mix-bonded framework,HOF-21 possessed moderate pore size of 0.35 nm and displayed excellent stability under humid feed gas.The chemical functions of multiple binding sites and continuous hydrogen-bonded network jointly facilitated the mass transport of CO_(2).The resulting HOF-21 mixed-matrix membrane exhibited a permeability above 750 Barrer,a selectivity of~40 for CO_(2)/CH_(4) and~60 for CO_(2)/N_(2),surpassing the 2008 Robeson upper bound.This work enlarges the family of mixed-matrix membranes and lays the foundation for HOF membrane development.展开更多
Potato is the thirdmost important food crop in theworld.Diverse pathogens threaten sustainable crop production but can be controlled,in many cases,through the deployment of disease resistance genes belonging to the fa...Potato is the thirdmost important food crop in theworld.Diverse pathogens threaten sustainable crop production but can be controlled,in many cases,through the deployment of disease resistance genes belonging to the family of nucleotide-binding,leucine-rich-repeat(NLR)genes.To identify effective disease resistance genes in established varieties,we have successfully established SMRT-AgRenSeq in tetraploid potatoes and have further enhanced the methodology by including dRenSeq in an approach that we term SMR-AgRenSeq-d.The inclusion of dRenSeq enables the filtering of candidates after the association analysis by establishing a presence/absence matrix across resistant and susceptible varieties that is translated into an F1 score.Using a SMRT-RenSeq-based sequence representation of the NLRome from the cultivar Innovator,SMRT-AgRenSeq-d analyses reliably identified the late blight resistance benchmark genes Rpi-R1,Rpi-R2-like,Rpi-R3a,and Rpi-R3b in a panel of 117 varieties with variable phenotype penetrations.All benchmark genes were identified with an F1 score of 1,which indicates absolute linkage in the panel.This method also identified nine strong candidates for Gpa5 that controls the potato cyst nematode(PCN)species Globodera pallida(pathotypes Pa2/3).Assuming that NLRs are involved in controlling many types of resistances,SMRT-AgRenSeq-d can readily be applied to diverse crops and pathogen systems.展开更多
基金supported in part by the National Natural Science Foundation of China(62273255,62350003,62088101)the Shanghai Science and Technology Cooperation Project(22510712000,21550760900)+1 种基金the Shanghai Municipal Science and Technology Major Project(2021SHZDZX0100)the Fundamental Research Funds for the Central Universities
文摘Dear Editor,This letter is concerned with the problem of time-varying formation tracking for heterogeneous multi-agent systems(MASs) under directed switching networks. For this purpose, our first step is to present some sufficient conditions for the exponential stability of a particular category of switched systems.
基金supported by the National Natural Science Foundation of China(No.82271515)the SJTU Trans-Med Awards Research(No.2019015)+4 种基金the Scientific and Technological Innovation Action Plan of Shanghai(No.KY20211478)the Shanghai Municipal Science and Technology Major Project(No.2021SHZDZX)the Nursing Development Program of Shanghai Jiao Tong University School of Medicine(No.SJTUHLXK2022)the 2024 Shanghai Ruijin Hospital Nursing Research Fund(No.RJHK-2024-001)the Shanghai Nursing Association Funding(No.2024MS-B13),China。
文摘Abuse of amphetamine-based stimulants is a primary public health concern.Recent studies have underscored a troubling escalation in the inappropriate use of prescription amphetamine-based stimulants.However,the neurophysiological mechanisms underlying the impact of acute methamphetamine exposure(AME)on sleep homeostasis remain to be explored.This study employed non-human primates and electroencephalogram(EEG)sleep staging to evaluate the influence of AME on neural oscillations.The primary focus was on alterations in spindles,delta oscillations,and slow oscillations(SOs)and their interactions as conduits through which AME influences sleep stability.AME predominantly diminishes sleep-spindle waves in the non-rapid eye movement 2(NREM2)stage,and impacts SOs and delta waves differentially.Furthermore,the competitive relationships between SO/delta waves nesting with sleep spindles were selectively strengthened by methamphetamine.Complexity analysis also revealed that the SO-nested spindles had lost their ability to maintain sleep depth and stability.In summary,this finding could be one of the intrinsic electrophysiological mechanisms by which AME disrupted sleep homeostasis.
基金funded by the National Natural Science Foundation of China(No.8207286).
文摘Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.
基金National Natural Science Foundation of China(52073278)the“Medical Science+X”Cross-innovation Team of the Norman Bethune Health Science of Jilin University(2022JBGS10)+2 种基金the Jilin Province Science and Technology Development Program(20190201044JC20230101045JC)the Education Department of Jilin Province(JJKH20231205KJ).
文摘Biological nanotechnologies based on functional nanoplatforms have synergistically catalyzed the emergence of cancer therapies.As a subtype of metal-organic frameworks(MOFs),zeolitic imidazolate frameworks(ZIFs)have exploded in popularity in the field of biomaterials as excellent protective materials with the advantages of conformational flexibility,thermal and chemical stability,and functional controllability.With these superior properties,the applications of ZIF-based materials in combination with various therapies for cancer treatment have grown rapidly in recent years,showing remarkable achievements and great potential.This review elucidates the recent advancements in the use of ZIFs as drug delivery agents for cancer therapy.The structures,synthesis methods,properties,and various modifiers of ZIFs used in oncotherapy are presented.Recent advances in the application of ZIF-based nanoparticles as single or combination tumor treatments are reviewed.Furthermore,the future prospects,potential limitations,and challenges of the application of ZIF-based nanomaterials in cancer treatment are discussed.We except to fully explore the potential of ZIF-based materials to present a clear outline for their application as an effective cancer treatment to help them achieve early clinical application.
基金Supported by National Natural Science Foundation of China(Grant Nos.52375414,52075100)Shanghai Science and Technology Committee Innovation Grant of China(Grant No.23ZR1404200).
文摘In modern industrial design trends featuring with integration,miniaturization,and versatility,there is a growing demand on the utilization of microstructural array devices.The measurement of such microstructural array components often encounters challenges due to the reduced scale and complex structures,either by contact or noncontact optical approaches.Among these microstructural arrays,there are still no optical measurement methods for micro corner-cube reflector arrays.To solve this problem,this study introduces a method for effectively eliminating coherent noise and achieving surface profile reconstruction in interference measurements of microstructural arrays.The proposed denoising method allows the calibration and inverse solving of system errors in the frequency domain by employing standard components with known surface types.This enables the effective compensation of the complex amplitude of non-sample coherent light within the interferometer optical path.The proposed surface reconstruction method enables the profile calculation within the situation that there is complex multi-reflection during the propagation of rays in microstructural arrays.Based on the measurement results,two novel metrics are defined to estimate diffraction errors at array junctions and comprehensive errors across multiple array elements,offering insights into other types of microstructure devices.This research not only addresses challenges of the coherent noise and multi-reflection,but also makes a breakthrough for quantitively optical interference measurement of microstructural array devices.
基金supported by the National Natural Science Foundation of China(Nos.52170156,52250056,and 52293443)the Shenzhen Science and Technology Program(No.KQTD20190929172630447).
文摘Harnessing bacteria for superoxide production in bioremediation holds immense promise,yet its practical application is hindered by slow production rates and the relatively weak redox potential of superoxide.This study delves into a cost-effective approach to amplify superoxide production using an Arthrobacter strain,a prevalent soil bacterial genus.Our research reveals that introducing a carbon source along with specific iron-binding ligands,including deferoxamine(DFO),diethylenetriamine pentaacetate(DTPA),citrate,and oxalate,robustly augments microbial superoxide generation.Moreover,our findings suggest that these iron-binding ligands play a pivotal role in converting superoxide into hydroxyl radicals by modulating the electron transfer rate between Fe(Ⅲ)/Fe(Ⅱ)and superoxide.Remarkably,among the tested ligands,only DTPA emerges as a potent promoter of this conversion process when complexed with Fe(Ⅲ).We identify an optimal Fe(Ⅲ)to DTPA ratio of approximately 1:1 for enhancing hydroxyl radical production within the Arthrobacter culture.This research underscores the efficacy of simultaneously introducing carbon sources and DTPA in facilitating superoxide production and its subsequent conversion to hydroxyl radicals,significantly elevating bioremediation performance.Furthermore,our study reveals that DTPA augments superoxide production in cultures of diverse soils,with various soil microorganisms beyond Arthrobacter identified as contributors to superoxide generation.This emphasizes the universal applicability of DTPA across multiple bacterial genera.In conclusion,our study introduces a promising methodology for enhancing microbial superoxide production and its conversion into hydroxyl radicals.These findings hold substantial implications for the deployment of microbial reactive oxygen species in bioremediation,offering innovative solutions for addressing environmental contamination challenges.
基金supported by the Natural Science Foundation of Guangdong Province(2023A1515030211)the National Natural Science Foundation of China(22278157)Guangzhou Science and Technology Program(2023B03J1365).
文摘A critical pathway towards enhancing pulp mill biorefineries is to integrate the extraction and utilization of hemicelluloses into the pulping processes.Hence,an industrial pre-extraction strategy for hemicelluloses targeting eucalyptus kraft pulping process was developed.Alkaline solution or pulping white liquor was used to pre-extract hemicelluloses before the actual pulping process.The response surface methodology(RSM)technique was applied to investigate the most suitable conditions to maximize the yield of these hemicelluloses while simultaneously minimizing the damage to pulp yields and properties.Temperature(105 to 155℃),alkali concentration(3%to 8%),sulfidity(20%to 30%)and retention time(19 to 221 min)were combined to evaluate their effects on hemicellulose yields and chemical structures.The optimal pre-extraction conditions identified in this work(5.75%NaOH concentration,25%sulfidity at 135℃for 60 min)successfully allowed recovering 4.8%of hemicelluloses(based on the wood dry mass)and limited damages to pulp yields and properties.The cellulose content in pulp can even be increased by about 10%.Hemicellulose emulsification properties were also evaluated,which were comparable to synthetic emulsifiers.This study provides an industrial pathway to effectively separate and utilize wood hemicelluloses from the pulping process,which has the potential to improve the economy and material utilization of pulp and paper mills.
基金supported by the National Natural Science Foundation of China(Grant No.32171367)Natural Science Foundation of Jiangsu Province(Grant No.BK20230236)+1 种基金Science and Technology Project of Suzhou(Grant No.SS202135)CAS-VPST Silk Road Science Fund 2021(Grant No.121E32KYSB20200021).
文摘Mesenchymal stem cells(MSCs)have emerged as promising candidates for idiopathic pulmonary fibrosis(IPF)therapy.Increasing the MSC survival rate and deepening the understanding of the behavior of transplanted MSCs are of great significance for improving the efficacy of MSC-based IPF treatment.Therefore,dual-functional Au-based nanoparticles(Au@PEG@PEI@TAT NPs,AuPPT)were fabricated by sequential modification of cationic polymer polyetherimide(PEI),polyethylene glycol(PEG),and transactivator of transcription(TAT)penetration peptide on AuNPs,to co-deliver retinoic acid(RA)and microRNA(miRNA)for simultaneously enhancing MSC survive and real-time imaging tracking of MSCs during IPF treatment.AuPPT NPs,with good drug loading and cellular uptake abilities,could efficiently deliver miRNA and RA to protect MSCs from reactive oxygen species and reduce their expression of apoptosis executive protein Caspase 3,thus prolonging the survival time of MSC after transplantation.In themeantime,the intracellular accumulation of AuPPT NPs enhanced the computed tomography imaging contrast of transplantedMSCs,allowing them to be visually tracked in vivo.This study establishes an Au-based dual-functional platform for drug delivery and cell imaging tracking,which provides a new strategy for MSC-related IPF therapy.
基金financially supported by the National Natural Science Foundation of China(Nos.92168106 and 82222039).
文摘Macrophage immunotherapy represents an emerging therapeutic approach aimed at modulating the immune response to alleviate disease symptoms.Nanomaterials(NMs)have been engineered to monitor macrophage metabolism,enabling the evaluation of disease progression and the replication of intricate physiological signal patterns.They achieve this either directly or by delivering regulatory signals,thereby mapping phenotype to effector functions through metabolic repurposing to customize macrophage fate for therapy.However,a comprehensive summary regarding NM-mediated macrophage visualization and coordinated metabolic rewiring to maintain phenotypic equilibrium is currently lacking.This review aims to address this gap by outlining recent advancements in NM-based metabolic immunotherapy.We initially explore the relationship between metabolism,polarization,and disease,before delving into recent NM innovations that visualize macrophage activity to elucidate disease onset and fine-tune its fate through metabolic remodeling for macrophage-centered immunotherapy.Finally,we discuss the prospects and challenges of NM-mediated metabolic immunotherapy,aiming to accelerate clinical translation.We anticipate that this review will serve as a valuable reference for researchers seeking to leverage novel metabolic intervention-matched immunomodulators in macrophages or other fields of immune engineering.
文摘Following publication of the original article[1],the authors reported an error in the last author’s name,it was mistakenly written as“Jun Den”.The correct author’s name“Jun Deng”has been updated in this Correction.
基金funded by the National Natural Science Foundation of China(No.51974356).
文摘Accurately predicting the production rate and estimated ultimate recovery(EUR)of shale oil wells is vital for efficient shale oil development.Although numerical simulations provide accurate predictions,their high time,data,and labor demands call for a swifter,yet precise,method.This study introduces the DuongeCNNeLSTM(D-C-L)model,which integrates a convolutional neural network(CNN)with a long short-term memory(LSTM)network and is grounded on the empirical Duong model for physical constraints.Compared to traditional approaches,the D-C-L model demonstrates superior precision,efficiency,and cost-effectiveness in predicting shale oil production.
基金supported by an unrestricted,investigator-initiated research grant by Scenery(BS),which provided the devices used.The project was sponsored by SJTU Trans-med Awards Research(2019015 to BS)Shanghai Clinical Research Centre for Mental Health(19MC191100 to BS)+3 种基金sponsored by the National Natural Science Foundation of China(81771482)supported by the Guangci Professorship Programme of Ruijin Hospital(N/A)and a Medical Research Council Senior Clinical Fellowship(MR/P008747/1)sponsored by the National Natural Science Foundation of China(82101546)the Shanghai Sailing Program(21YF1426700).The funding sources were not involved in the design and conduct of the study。
文摘Background Structural imaging holds great potential for precise targeting and stimulation for deep brain stimulation(DBS).The anatomical information it provides may serve as potential biomarkers for predicting the efficacy of DBS in treatment-resistant depression(TRD).Aims The primary aim is to identify preoperative imaging biomarkers that correlate with the efficacy of DBS in patients with TRD.Methods Preoperative imaging parameters were estimated and correlated with the 6-month clinical outcome of patients with TRD receiving combined bed nucleus of the stria terminalis(BNST)-nucleus accumbens(NAc)DBS.White matter(WM)properties were extracted and compared between the response/non-response and remission/non-remission groups.Structural connectome was constructed and analysed using graph theory.Distances of the volume of activated tissue(VAT)to the main modulating tracts were also estimated to evaluate the correlations.Results Differences in fibre bundle properties of tracts,including superior thalamic radiation and reticulospinal tract,were observed between the remission and nonremission groups.Distance of the centre of the VAT to tracts connecting the ventral tegmental area and the anterior limb of internal capsule on the left side varied between the remission and non-remission groups(p=0.010,t=3.07).The normalised clustering coefficient(γ)and the small-world property(σ)in graph analysis correlated with the symptom improvement after the correction of age.Conclusions Presurgical structural alterations in WM tracts connecting the frontal area with subcortical regions,as well as the distance of the VAT to the modulating tracts,may influence the clinical outcome of BNST-NAc DBS.These findings provide potential imaging biomarkers for the DBS treatment for patients with TRD.
基金supported by the University Grants Committee/Research Grants Council of the Hong Kong Special Administrative Region,China (Grant No.AoE/P-502/20,CRF Project:C5031-22G and C1015-21E,and GRF Project:15303521,11310522,11305223,11300123,16304020,and 16306521)the Department of Science and Technology of Guangdong Province (Grant No.2020B1515120073)+1 种基金the City University of Hong Kong (Grant Nos.9380131,9610628,and 7005867)the National Key R&D Program of China (Grant No.2022YFA1404700).
文摘Quantum technologies rely on creating and manipulating entangled sources,which are essential for quantum information,communication,and imaging.By integrating quantum technologies and all-dielectric metasurfaces,the performance of miniature display devices can be enhanced to a higher level.Miniature display technology,such as virtual reality display,has achieved original commercial success,and was initially applied to immersive games and interactive scenes.While the consumer market has quickly adopted this technology,several areas remain for improvement,including concerns around bulkiness,dual-channel display,and noise reduction.Here,we experimentally realize a quantum meta-hologram concept demonstration of a miniature display.We fabricate an ultracompact meta-hologram based on 1μm thick titanium dioxide(TiO_(2)).The meta-hologram can be remotely switched with heralding technique and is robust against noise with the quantum entangled source.The platform can alter the miniature display channel by manipulating heralding photons’polarization,removing speckles and multiple reflective light noise,improving imaging contrast,and potentially decreasing device weight.Imaging contrast increases from 0.36 dB under speckle noise influences to 6.8 dB in quantum correlation imaging.This approach has the potential to miniaturize quantum displays and quantum communication devices.
基金supported by the National Natural Science Foundation of China(Nos.52170156,52250056,and 52293442)the Shenzhen Science and Technology Program(No.KQTD20190929172630447)。
文摘Effective monitoring and management of microbial risk factors in wastewater treatment plants(WWTPs)effluents require a comprehensive investigation of these risks.A global survey on microbial risk factors in WWTP effluents could reveal important insights into their risk features.This study aims to explore the abundance and types of antibiotic resistance genes(ARGs),virulence factor genes(VFGs),the vector of ARG/VFG,and dominant pathogens in global WWTP effluents.We collected 113 metagenomes of WWTP effluents from the Sequence Read Archive of the National Center for Biotechnology Information and characterized the microbial risk factors.Our results showed that multidrug resistance was the dominant ARG type,while offensive virulence factors were the most abundant type of VFGs.The most dominant types of ARGs in the vector of plasmid and phage were both aminoglycoside resistance,which is concerning as aminoglycosides are often a last resort for treating multi-resistant infections.Acinetobacter baumannii was the most dominant pathogen,rather than Escherichia coli,and a weak negative correlation between Escherichia coli and two other dominant pathogens(Acinetobacter baumannii and Bacteroides uniformis)suggests that using Escherichia coli as a biological indicator for all pathogens in WWTP effluents may not be appropriate.The Getah virus was the most dominant virus found in global WWTP effluents.Our study presents a comprehensive global-scale investigation of microbial risk factors in WWTP effluents,providing valuable insights into the potential risks associated with WWTP effluents and contributing to the monitoring and control of these risks.
基金financially supported by the National Natural Science Foundation of China(No.52072322)the Department of Science and Technology of Sichuan Province,China(Nos.23GJHZ0147,23ZDYF0262,2022YFG0294,and 2019-GH02-00052-HZ)。
文摘Electrochemical lithium extraction from salt lakes is an effective strategy for obtaining lithium at a low cost.Nevertheless,the elevated Mg:Li ratio and the presence of numerous coexisting ions in salt lake brines give rise to challenges,such as prolonged lithium extraction periods,diminished lithium extraction efficiency,and considerable environmental pollution.In this work,Li FePO4(LFP)served as the electrode material for electrochemical lithium extraction.The conductive network in the LFP electrode was optimized by adjusting the type of conductive agent.This approach resulted in high lithium extraction efficiency and extended cycle life.When the single conductive agent of acetylene black(AB)or multiwalled carbon nanotubes(MWCNTs)was replaced with the mixed conductive agent of AB/MWCNTs,the average diffusion coefficient of Li+in the electrode increased from 2.35×10^(-9)or 1.77×10^(-9)to 4.21×10^(-9)cm^(2)·s^(-1).At the current density of 20 mA·g^(-1),the average lithium extraction capacity per gram of LFP electrode increased from 30.36 mg with the single conductive agent(AB)to 35.62 mg with the mixed conductive agent(AB/MWCNTs).When the mixed conductive agent was used,the capacity retention of the electrode after 30 cycles reached 82.9%,which was considerably higher than the capacity retention of 65.8%obtained when the single AB was utilized.Meanwhile,the electrode with mixed conductive agent of AB/MWCNTs provided good cycling performance.When the conductive agent content decreased or the loading capacity increased,the electrode containing the mixed conductive agent continued to show excellent electrochemical performance.Furthermore,a self-designed,highly efficient,continuous lithium extraction device was constructed.The electrode utilizing the AB/MWCNT mixed conductive agent maintained excellent adsorption capacity and cycling performance in this device.This work provides a new perspective for the electrochemical extraction of lithium using LFP electrodes.
基金supported by the National Key Research and Development Program of China(No.2022YFA1504100)the National Natural Science Foundation of China(Nos.22005215,22279089,and 22178251).
文摘Nickel-rich layered oxide cathode(LiNi_(x)Co_(y)Mn_(1−x−y)O_(2),x>0.5,NCM)shows substantial potential for applications in longer-range electrical vehicles.However,the rapid capacity decay and serious safety concerns impede its practical viability.This work provides a hydrogen-bonded organic framework(HOF)modification strategy to simultaneously improve the electrochemical performance,thermal stability and incombustibility of separator.Melamine cyanurate(MCA),as a low-cost and reliable flame-retardant HOF,was implemented in the separator modification layer,which can prevent the battery short circuit even at a high temperature.In addition,the supermolecule properties of MCA provide unique physical and chemical microenvironment for regulating ion-transport behavior in electrolyte.The MCA coating layer enabled the nickel-rich layered oxide cathode with a high-capacity retention of 90.3%after 300 cycles at 1.0 C.Collectively,the usage of MCA in lithium-ion batteries(LIBs)affords a simple,low-cost and efficient strategy to improve the security and service life of nickel-rich layered cathodes.
基金sponsored by National Natural Science Foundation of China (82303396,82022051,82072972 and 81672883)Science and Technology Commission of Shanghai (22YF1408400)+6 种基金Shanghai Pilot Program for Basic Research (TQ20240208)Natural Science Foundation of Chongqing (CSTB2024NSCQ-JQX0009 and CSTB2024NSCQ-MSX0594)The Postdoctoral Foundation of China (2023T160122)Anti-cancer Association in Shanghai,Joint Foundation from Fudan University Shanghai Cancer Center (YJQN202102)Science&Techenology Department of Sichuan Province (2023NSFSC0705)the Fund of major military joint research project (2019LH 02)Shanghai municipal hospital emerging frontier joint research project (SHDC12024112)。
文摘While KRAS mutation is the leading cause of low survival rates in lung cancer bone metastasis patients,effective treatments are still lacking.Here,we identified homeobox C10(HOXC10)as a lynchpin in pan-KRAS-mutant lung cancer bone metastasis.
基金Supported by National Natural Science Foundation of China(Grant No.50875171)National Hi-tech Research and Development Program of China(863 Program,Grant No.2009AA04Z150)
文摘A numerical control (NC) tool path of digital CAD model is widely generated as a set of short line segments in machining. However, there are three shortcomings in the linear tool path, such as discontinuities of tangency and curvature, huge number of line segments, and short lengths of line segments. These disadvantages hinder the development of high speed machining. To smooth the linear tool path and improve machining efficiency of short line segments, this paper presents an optimal feed interpolator based on G^2 continuous Bézier curves for the linear tool path. First, the areas suitable for fitting are screened out based on the geometric characteristics of continuous short segments (CSSs). CSSs in every area are compressed and fitted into a G^2 Continuous Bézier curve by using the least square method. Then a series of cubic Bézier curves are generated. However, the junction between adjacent Bézier curves is only G^0 continuous. By adjusting the control points and inserting Bézier transition curves between adjacent Bézier curves, the G^2 continuous tool path is constructed. The fitting error is estimated by the second-order Taylor formula. Without iteration, the fitting algorithm can be implemented in real-time environment. Second, the optimal feed interpolator considering the comprehensive constraints (such as the chord error constraint, the maximum normal acceleration, servo capacity of each axis, etc.) is proposed. Simulation and experiment are conducted. The results shows that the proposed method can generate smooth path, decrease the amount of segments and reduce machining time for machining of linear tool path. The proposed research provides an effective method for high-speed machining of complex 2-D/3-D profiles described by short line segments.
基金support from National Key Research and Development Program of China(No.2021YFB3802200)National Natural Science Foundation of China(No.U20B2023,22208238,U1732120)+1 种基金the Haihe Laboratory of Sustainable Chemical TransformationsNingbo Natural Science Foundation(No.2021J004).
文摘Hydrogen-bonded organic frameworks(HOFs)have emerged as a new class of crystalline porous materials,and their application in membrane technology needs to be explored.Herein,for the first time,we demonstrated the utilization of HOF-based mixed-matrix membrane for CO_(2) separation.HOF-21,a unique metallo-hydrogen-bonded organic framework material,was designed and processed into nanofillers via amine modulator,uniformly dispersing with Pebax polymer.Featured with the mix-bonded framework,HOF-21 possessed moderate pore size of 0.35 nm and displayed excellent stability under humid feed gas.The chemical functions of multiple binding sites and continuous hydrogen-bonded network jointly facilitated the mass transport of CO_(2).The resulting HOF-21 mixed-matrix membrane exhibited a permeability above 750 Barrer,a selectivity of~40 for CO_(2)/CH_(4) and~60 for CO_(2)/N_(2),surpassing the 2008 Robeson upper bound.This work enlarges the family of mixed-matrix membranes and lays the foundation for HOF membrane development.
基金supported by the Rural&Environment Science&Analytical Services(RESAS)Division of the Scottish Government through project JHI-B1-1,the Biotechnology and Biological Sciences Research Council(BBSRC)through award BB/S015663/1 and the Royal Society through award NAF\R1\201061YW was supported through the CSC scholarship program,China.LB was supported through the East of Scotland Bioscience Doctoral Training Partnership(EASTBIO DTP)funded by the BBSRC award BB/T00875X/1.AK was supported through a Research Leaders 2025 fellowship funded by European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement no.754380.
文摘Potato is the thirdmost important food crop in theworld.Diverse pathogens threaten sustainable crop production but can be controlled,in many cases,through the deployment of disease resistance genes belonging to the family of nucleotide-binding,leucine-rich-repeat(NLR)genes.To identify effective disease resistance genes in established varieties,we have successfully established SMRT-AgRenSeq in tetraploid potatoes and have further enhanced the methodology by including dRenSeq in an approach that we term SMR-AgRenSeq-d.The inclusion of dRenSeq enables the filtering of candidates after the association analysis by establishing a presence/absence matrix across resistant and susceptible varieties that is translated into an F1 score.Using a SMRT-RenSeq-based sequence representation of the NLRome from the cultivar Innovator,SMRT-AgRenSeq-d analyses reliably identified the late blight resistance benchmark genes Rpi-R1,Rpi-R2-like,Rpi-R3a,and Rpi-R3b in a panel of 117 varieties with variable phenotype penetrations.All benchmark genes were identified with an F1 score of 1,which indicates absolute linkage in the panel.This method also identified nine strong candidates for Gpa5 that controls the potato cyst nematode(PCN)species Globodera pallida(pathotypes Pa2/3).Assuming that NLRs are involved in controlling many types of resistances,SMRT-AgRenSeq-d can readily be applied to diverse crops and pathogen systems.
