Background Myocardial infarction(MI)and postmyocardial remodeling are the most common causes of heart failure worldwide and seriously affect the quality of life and prognosis of patients.Dapagliflozin,a sodium glucose...Background Myocardial infarction(MI)and postmyocardial remodeling are the most common causes of heart failure worldwide and seriously affect the quality of life and prognosis of patients.Dapagliflozin,a sodium glucose cotransporter 2(SGLT2)inhibitor,is a novel class of hypoglycemic drug that has been proven to have cardiovascular protective effects.However,the underlying mechanisms by which dapagliflozin affects MI have yet to be elucidated.Methods An MI mouse model was created by ligating the left anterior descending branch of the coronary artery.Hematoxylin‒eosin(HE)and Masson’s trichrome(Masson)staining were used to assess myocardial damage.The levels of fibrosis-related and cuproptosis-related markers were assessed via Western blot analysis.A hypoxia-induced cardiomyocyte fibrosis model was constructed in vitro.The DCFH-DA probe was used to measure the levels of reactive oxygen species(ROS),and flow cytometry was used to identify cell apoptosis.Results Dapagliflozin improved heart function,ameliorated fibrosis in the myocardium,and alleviated myocardial injury.Moreover,dapagliflozin reduced the copper ion concentration and ROS accumulation and inhibited the expression of cuproptosis-related markers.Dapagliflozin suppressed the expression of HIF-1α/TGF-βsignal and the overexpression of HIF-1αeffectively reversed the dapagliflozin-mediated myocardial protective effects.Conclusion Dapagliflozin reduced myocardial fibrosis by suppressing HIF-1α/TGF-β-mediated cuproptosis.展开更多
BACKGROUND A healthy body shape is essential to maintain athletes’sports level.At present,little is known about the effect of athletes’body shape on anterior cruciate ligament reconstruction(ACLR).Moreover,the relat...BACKGROUND A healthy body shape is essential to maintain athletes’sports level.At present,little is known about the effect of athletes’body shape on anterior cruciate ligament reconstruction(ACLR).Moreover,the relationship between body shape and variables such as knee joint function after operation and return to the field has not been well studied.AIM To verify the relationship between a body shape index(ABSI)and the functional prognosis of the knee after ACLR in athletes with ACL injuries.METHODS We reviewed 76 athletes with unilateral ACL ruptures who underwent ACLR surgery in the First Hospital of Shanxi Medical University between 2017 and 2020,with a follow-up period of more than 24 mo.First,all populations were divided into a High-ABSI group(ABSI>0.835,n=38)and a Low-ABSI group(ABSI<0.835,n=38)based on the arithmetic median(0.835)of ABSI values.The primary exposure factor was ABSI,and the outcome indicators were knee function scores as well as postoperative complications.The correlation between ABSI and postoperative knee function scores and postoperative complications after ACLR were analyzed using multifactorial logistic regression.RESULTS The preoperative knee function scores of the two groups were similar.The surgery and postoperative rehabilitation exercises,range of motion(ROM)compliance rate,Lysholm score,and Knee Injury and Osteoarthritis Outcome Score of the two groups gradually increased,whereas the quadriceps atrophy index gradually decreased.The knee function scores were higher in the Low-ABSI group than in the High-ABSI group at the 24-mo postoperative follow-up(P<0.05).In multifactorial logistic regression,ABSI was a risk factor of low knee joint function score after surgery,specifically low ROM scores(odds ratio[OR]=1.31,95%confidence interval[CI][1.10-1.44];P<0.001),low quadriceps atrophy index(OR=1.11,95%CI[0.97-1.29];P<0.05),low Lysholm scores(OR=2.34,95%CI[1.78-2.94];P<0.001),low symptoms(OR=1.14,95%CI[1.02-1.34];P<0.05),low activity of daily living(OR=1.34,95%CI[1.18-1.65];P<0.05),low sports(OR=2.47,95%CI[1.78-2.84];P<0.001),and low quality of life(OR=3.34,95%CI[2.88-3.94];P<0.001).ABSI was also a risk factor for deep vein thrombosis of the lower limb(OR=2.14,95%CI[1.88-2.36],P<0.05]and ACL recurrent rupture(OR=1.24,95%CI[0.98-1.44],P<0.05)after ACLR.CONCLUSION ABSI is a risk factor for the poor prognosis of knee function in ACL athletes after ACLR,and the risk of poor knee function after ACLR,deep vein thrombosis of lower limb,and ACL recurrent rupture gradually increases with the rise of ABSI.展开更多
China's salt lakes hold significant lithium reserves,yet the development of low-concentration lithium resources from the carbonate brines in Xizang is a pressing matter.Alkylβ-diketones extractants are capable of...China's salt lakes hold significant lithium reserves,yet the development of low-concentration lithium resources from the carbonate brines in Xizang is a pressing matter.Alkylβ-diketones extractants are capable of efficiently extracting lithium from alkaline solutions using an extraction method,but its optimized extraction prerequisite is an extreme alkaline solution with higher pH.This research introduces a process for the effective extraction and separation of lithium and sodium from the carbonate brine of Jieze Caka Salt Lake,utilizing trifluorinatedβ-biketone HB TA(4,4,4-trifluoro-1-phenyl-1,3-butanedione)-TBP(tributyl phosphate)/kerosene system.A threestage extraction yielded 98.06%of Li^(+)and 1.69%of Na^(+)from the initial brine with a lower pH of 8.961 and a separation factor(β_(Li/Na))of 2948.The simplified process was then implemented using organic direct recycling without regeneration and scrubbing raffinate reflux for a total of 23 cycles,thereby demonstrating the exceptional effectiveness and stability of this system.The resulting lithium-rich stripping solution,with the Li/Na mass ratio amplified by 747 times,underwent further magnesium removal and precipitation using sodium carbonate to yield a high-purity lithium carbonate product of 99.50%.This study offers a novel approach and technology for the efficient separation of lithium ion directly from a partialneutral carbonate salt lake with a high Na/Li ratio and low lithium concentration.展开更多
基金supported by Fujian Provincial Natural Science Foundation of China(No.2022J011489 and No.2023J011876)Startup Fund for Scientific Research of Fujian Medical University(No.2021QH1210).
文摘Background Myocardial infarction(MI)and postmyocardial remodeling are the most common causes of heart failure worldwide and seriously affect the quality of life and prognosis of patients.Dapagliflozin,a sodium glucose cotransporter 2(SGLT2)inhibitor,is a novel class of hypoglycemic drug that has been proven to have cardiovascular protective effects.However,the underlying mechanisms by which dapagliflozin affects MI have yet to be elucidated.Methods An MI mouse model was created by ligating the left anterior descending branch of the coronary artery.Hematoxylin‒eosin(HE)and Masson’s trichrome(Masson)staining were used to assess myocardial damage.The levels of fibrosis-related and cuproptosis-related markers were assessed via Western blot analysis.A hypoxia-induced cardiomyocyte fibrosis model was constructed in vitro.The DCFH-DA probe was used to measure the levels of reactive oxygen species(ROS),and flow cytometry was used to identify cell apoptosis.Results Dapagliflozin improved heart function,ameliorated fibrosis in the myocardium,and alleviated myocardial injury.Moreover,dapagliflozin reduced the copper ion concentration and ROS accumulation and inhibited the expression of cuproptosis-related markers.Dapagliflozin suppressed the expression of HIF-1α/TGF-βsignal and the overexpression of HIF-1αeffectively reversed the dapagliflozin-mediated myocardial protective effects.Conclusion Dapagliflozin reduced myocardial fibrosis by suppressing HIF-1α/TGF-β-mediated cuproptosis.
文摘BACKGROUND A healthy body shape is essential to maintain athletes’sports level.At present,little is known about the effect of athletes’body shape on anterior cruciate ligament reconstruction(ACLR).Moreover,the relationship between body shape and variables such as knee joint function after operation and return to the field has not been well studied.AIM To verify the relationship between a body shape index(ABSI)and the functional prognosis of the knee after ACLR in athletes with ACL injuries.METHODS We reviewed 76 athletes with unilateral ACL ruptures who underwent ACLR surgery in the First Hospital of Shanxi Medical University between 2017 and 2020,with a follow-up period of more than 24 mo.First,all populations were divided into a High-ABSI group(ABSI>0.835,n=38)and a Low-ABSI group(ABSI<0.835,n=38)based on the arithmetic median(0.835)of ABSI values.The primary exposure factor was ABSI,and the outcome indicators were knee function scores as well as postoperative complications.The correlation between ABSI and postoperative knee function scores and postoperative complications after ACLR were analyzed using multifactorial logistic regression.RESULTS The preoperative knee function scores of the two groups were similar.The surgery and postoperative rehabilitation exercises,range of motion(ROM)compliance rate,Lysholm score,and Knee Injury and Osteoarthritis Outcome Score of the two groups gradually increased,whereas the quadriceps atrophy index gradually decreased.The knee function scores were higher in the Low-ABSI group than in the High-ABSI group at the 24-mo postoperative follow-up(P<0.05).In multifactorial logistic regression,ABSI was a risk factor of low knee joint function score after surgery,specifically low ROM scores(odds ratio[OR]=1.31,95%confidence interval[CI][1.10-1.44];P<0.001),low quadriceps atrophy index(OR=1.11,95%CI[0.97-1.29];P<0.05),low Lysholm scores(OR=2.34,95%CI[1.78-2.94];P<0.001),low symptoms(OR=1.14,95%CI[1.02-1.34];P<0.05),low activity of daily living(OR=1.34,95%CI[1.18-1.65];P<0.05),low sports(OR=2.47,95%CI[1.78-2.84];P<0.001),and low quality of life(OR=3.34,95%CI[2.88-3.94];P<0.001).ABSI was also a risk factor for deep vein thrombosis of the lower limb(OR=2.14,95%CI[1.88-2.36],P<0.05]and ACL recurrent rupture(OR=1.24,95%CI[0.98-1.44],P<0.05)after ACLR.CONCLUSION ABSI is a risk factor for the poor prognosis of knee function in ACL athletes after ACLR,and the risk of poor knee function after ACLR,deep vein thrombosis of lower limb,and ACL recurrent rupture gradually increases with the rise of ABSI.
基金financially supported by the National Natural Science Foundation of China(No.U21A20305)Qinghai Provincial Department of Science and Technology(No.2022-ZJ-728)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2021430)。
文摘China's salt lakes hold significant lithium reserves,yet the development of low-concentration lithium resources from the carbonate brines in Xizang is a pressing matter.Alkylβ-diketones extractants are capable of efficiently extracting lithium from alkaline solutions using an extraction method,but its optimized extraction prerequisite is an extreme alkaline solution with higher pH.This research introduces a process for the effective extraction and separation of lithium and sodium from the carbonate brine of Jieze Caka Salt Lake,utilizing trifluorinatedβ-biketone HB TA(4,4,4-trifluoro-1-phenyl-1,3-butanedione)-TBP(tributyl phosphate)/kerosene system.A threestage extraction yielded 98.06%of Li^(+)and 1.69%of Na^(+)from the initial brine with a lower pH of 8.961 and a separation factor(β_(Li/Na))of 2948.The simplified process was then implemented using organic direct recycling without regeneration and scrubbing raffinate reflux for a total of 23 cycles,thereby demonstrating the exceptional effectiveness and stability of this system.The resulting lithium-rich stripping solution,with the Li/Na mass ratio amplified by 747 times,underwent further magnesium removal and precipitation using sodium carbonate to yield a high-purity lithium carbonate product of 99.50%.This study offers a novel approach and technology for the efficient separation of lithium ion directly from a partialneutral carbonate salt lake with a high Na/Li ratio and low lithium concentration.
