Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressi...Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.展开更多
Flexible,lightweight and high conductivity substrates are required for the development of next-generation flexible Li-ion batteries(LIBs).In addition,the interfacial strength between the active material and flexible s...Flexible,lightweight and high conductivity substrates are required for the development of next-generation flexible Li-ion batteries(LIBs).In addition,the interfacial strength between the active material and flexible substrate should be optimized for high-performance LIBs.Herein,cotton cloth(CC)is employed as a flexible substrate,and electroless plating is utilized to deposit a layer of Cu nanoparticles,which enhances the conductivity of CC and acts as a precursor for the active material,i.e.,CuO.The results reveal that the in situ etching and subsequent heat treatment converted Cu film into CuO nanowires on CC substrate.Moreover,carbon nano tubes(CNTs)are introduced to enhance the connectivity of CuO nano wires.Consequently,the CuO/CNT/CC electrode rendered a high areal capacity of>700μAh-cm^(-2)after100 charge/discharge cycles as well as excellent rate capability.The current work presents a novel route to develop desirable substrates for next-generation flexible Li-ion batteries.展开更多
The new fracturing technology of intensive stages and high-intensity proppant injection shows significant advantages of cost reduction and high efficiency in the volume fracturing of unconventional reservoirs,but it a...The new fracturing technology of intensive stages and high-intensity proppant injection shows significant advantages of cost reduction and high efficiency in the volume fracturing of unconventional reservoirs,but it also intensifies the erosion of the casing and the perforations,with a perforation diameter expansion and the casing wall thinning.To solve this problem,in view of the applications of the perforation fracturing technology under the construction conditions in Mahu Area in Xinjiang,China,the structure parameters of the downhole strings are measured and a calculation model of the perforation casing erosion is established for the large-displacement sand fracturing process in horizontal sections.The software ANSYS-FLUENT is used to simulate the casing and perforation erosion under different conditions of the displacement,the sand content,the proppant particle size,and the fracturing fluid viscosity in the sand fracturing process.The particle size of the proppant and the viscosity of the fracturing fluid mainly affect the erosion area,but they have little influence on the erosion rate.The sand content and the displacement significantly affect the erosion rate.Among these factors,the sand content has a more significant effect on the erosion rate and the displacement has a more significant effect on the erosion area.Due to the influence of gravity,the perforation erosion rate is larger when the phase angle ranges from 120°to 240°.展开更多
基金supported by Zhejiang Provincial Natural Science Foundation (No. Y206750)Zhejiang Foundation for Development of Science and Technology, China (No. 2008C33066)Wenzhou Foundation for Development of Science and Technology,China (No. H20060026)
文摘Objective： To investigate the expressions of caveolin-1, E-cadherin and β-catenin in gastric carcinoma, precancerous gastric and chronic non-atrophic gastritis tissues, and evaluate the correlation of these expressions with the development of gastric cancer. Methods： The expressions of caveolin-1, E-cadherin and β-catenin were detected by biotin-streptavidinperoxidase （SP） immunohistochemistry on 58 gastric cancer tissues, 40 precancerous gastric tissues and 42 chronic non-atrophic gastritis tissues. The correlation between the expressions of caveolin-1, E-cadherin and β-catenin, and the clinicopathologic parameters of gastric cancer was analyzed retrospectively. Results： The positive rates of caveolin-1 and E-cadherin expressions in gastric carcinoma were significantly lower than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. An abnormal rate of β-catenin expression in gastric carcinoma was higher than precancerous gastric and chronic non-atrophic gastritis tissues （P〈0.01）. Moreover, low expressions of caveolin-1, E-cadherin and β-catenin correlated with tumor size, depth of invasion, lymph node metastasis and TNM stage （P〈0.05）. The positive rates of caveolin-1 and E-cadherin expressions decreased （P〈0.01）, while an abnormal rate of β-catenin expression increased inversely, with the degree of atypical hyperplasia （P〈0.01）. Caveolin-1 expression correlated positively with E-cadherin （r=0.41, P〈0.05）. Caveolin-1 （r=-0.36, P〈0.05） and E-cadherin （r=-0.45, P〈0.05） expressions negatively correlated with abnormal β-catenin expression. Conclusion： These results suggested that dysregulated expressions of caveolin-1, E-cadherin and β-catenin correlated with the development of gastric cancer and its biological behavior.
基金the National Natural Science Foundation of China(Nos.21701022 and51690161)the Fundamental Research Funds for the Central Universities(Nos.N182505037 and N182410001)+2 种基金the Young Elite Scientists Sponsorship Program by CAST(No.2018QNRC001)the Liao Ning Revitalization Talents Program(No.XLYC1807214)the National Training Program of Innovation and Entrepreneurship for Undergraduates(No.201910145260)。
文摘Flexible,lightweight and high conductivity substrates are required for the development of next-generation flexible Li-ion batteries(LIBs).In addition,the interfacial strength between the active material and flexible substrate should be optimized for high-performance LIBs.Herein,cotton cloth(CC)is employed as a flexible substrate,and electroless plating is utilized to deposit a layer of Cu nanoparticles,which enhances the conductivity of CC and acts as a precursor for the active material,i.e.,CuO.The results reveal that the in situ etching and subsequent heat treatment converted Cu film into CuO nanowires on CC substrate.Moreover,carbon nano tubes(CNTs)are introduced to enhance the connectivity of CuO nano wires.Consequently,the CuO/CNT/CC electrode rendered a high areal capacity of>700μAh-cm^(-2)after100 charge/discharge cycles as well as excellent rate capability.The current work presents a novel route to develop desirable substrates for next-generation flexible Li-ion batteries.
基金the Sichuan Science and Technology Program(Grant No.21JCQN0066)the National Natural ScienceFoundation ofChina(GrantNo.51774249).
文摘The new fracturing technology of intensive stages and high-intensity proppant injection shows significant advantages of cost reduction and high efficiency in the volume fracturing of unconventional reservoirs,but it also intensifies the erosion of the casing and the perforations,with a perforation diameter expansion and the casing wall thinning.To solve this problem,in view of the applications of the perforation fracturing technology under the construction conditions in Mahu Area in Xinjiang,China,the structure parameters of the downhole strings are measured and a calculation model of the perforation casing erosion is established for the large-displacement sand fracturing process in horizontal sections.The software ANSYS-FLUENT is used to simulate the casing and perforation erosion under different conditions of the displacement,the sand content,the proppant particle size,and the fracturing fluid viscosity in the sand fracturing process.The particle size of the proppant and the viscosity of the fracturing fluid mainly affect the erosion area,but they have little influence on the erosion rate.The sand content and the displacement significantly affect the erosion rate.Among these factors,the sand content has a more significant effect on the erosion rate and the displacement has a more significant effect on the erosion area.Due to the influence of gravity,the perforation erosion rate is larger when the phase angle ranges from 120°to 240°.
