High-grade non-oriented silicon steel with higher permeability and lower core loss has become the pursed target with the rapid developnient of electrical machines.The effects of rare-earth(RE)element on recrystallizat...High-grade non-oriented silicon steel with higher permeability and lower core loss has become the pursed target with the rapid developnient of electrical machines.The effects of rare-earth(RE)element on recrystallization texture in RE-doped Fe-3.1 wt.%Si-1.2 wt.%Al non-oriented silicon steel were investigated by macro-and micro-texture analyses.Nonlinear variation of recrystallization texture with RE element content was observed.In the competition among main recrystallization texture components,favorable{113}<361>and X fiber(<001>//ND)are strengthened and unfavorable y fiber is evidently decreased by the addition of RE element,whereas the effect of RE element is reversed by excess RE element.The recrystallization texture development is determined by RE-affected nucleation and grain growth in terms of grain boundary migration related to segregation and inclusion.The properly inhibited grain boundary mobility can promote favorable recrystallization texture by the improved nucleation and efficient grain growth.展开更多
Background:Pulmonary arterial hypertension(PH)is a progressive disease with limited therapeutic options,ultimately leading to right heart failure and death.Recent findings indicate the role of the Warburg effect(aerob...Background:Pulmonary arterial hypertension(PH)is a progressive disease with limited therapeutic options,ultimately leading to right heart failure and death.Recent findings indicate the role of the Warburg effect(aerobic glycolysis)in the development of PH.However,the effect of the glycolysis inhibitor 3-bromopyruvate(3-BrPA)on the pathogenesis of PH has not been well investigated.This study aimed to determine whether 3-BrPA inhibits PH and its possible mechanism.Methods:PH was induced in adult Sprague-Dawley rats by a single intraperitoneal injection of monocrotaline(MCT).3-BrPA,or phosphate-buffered saline(PBS)was administered via intraperitoneal injection every other day from the first day of MCT-injection to 4 weeks of follow-up,and indices such as right ventricular systolic pressure(RVSP),right ventricular hypertrophy index(RVHI),pulmonary arteriolar remodeling indicated by percent media thickness(%MT),lactate levels and glucose consumption,were evaluated.Pulmonary arteriolar remodeling and right ventricular hypertrophy were observed in hematoxylin-eosin-stained lung sections.Western blotting,immunohistochemistry,and/or immunofluorescence analyses were used to measure the expression of relevant proteins.A cytochrome C release apoptosis assay and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling staining were used to measure cell apoptosis.Results:MCT-induced PH showed a significant increase in glucose consumption(0 vs.4 weeks:0.87±0.23 vs.2.94±0.47,P=0.0042)and lactate production(0 vs.4 weeks:4.19±0.34 vs.8.06±0.67,P=0.0004).Treatment with 3-BrPA resulted in a concomitant reduction in glucose consumption(1.10±0.35 vs.3.25±0.47,P=0.0063),lactate production(5.09±0.55 vs.8.06±0.67,P=0.0065),MCT-induced increase in RVSP(39.70±2.94 vs.58.85±2.32,P=0.0004),pulmonary vascular remodeling(%MT,43.45%±1.41%vs.63.66%±1.78%,P<0.0001),and right ventricular hypertrophy(RVHI,38.57%±2.69%vs.62.61%±1.57%,P<0.0001)when compared with those of the PBS-treated group.3-BrPA,a hexokinase 2 inhibitor,exerted its beneficial effect on PH by decreasing aerobic glycolysis and was also associated with inhibiting the expression of glucose transporter protein-1,inducing apoptosis,and suppressing inflammation.Conclusions:3-BrPA might have a potential beneficial effect on the PH treatment.展开更多
基金National Key R&D Program of China(Grant No.2016YFB0300305)National Natural Science Foundation of China(Grant Nos.51671049 and 51931002)the Postdoctoral Science Foundation of China(Grant No.2018M640257).
文摘High-grade non-oriented silicon steel with higher permeability and lower core loss has become the pursed target with the rapid developnient of electrical machines.The effects of rare-earth(RE)element on recrystallization texture in RE-doped Fe-3.1 wt.%Si-1.2 wt.%Al non-oriented silicon steel were investigated by macro-and micro-texture analyses.Nonlinear variation of recrystallization texture with RE element content was observed.In the competition among main recrystallization texture components,favorable{113}<361>and X fiber(<001>//ND)are strengthened and unfavorable y fiber is evidently decreased by the addition of RE element,whereas the effect of RE element is reversed by excess RE element.The recrystallization texture development is determined by RE-affected nucleation and grain growth in terms of grain boundary migration related to segregation and inclusion.The properly inhibited grain boundary mobility can promote favorable recrystallization texture by the improved nucleation and efficient grain growth.
基金This work was supported by grants from the National Natural Science Foundation of China(No.31600939)the Beijing Natural Science Foundation(No.7174280)the Beijing Talents Training Project(No.2015000020124G111).
文摘Background:Pulmonary arterial hypertension(PH)is a progressive disease with limited therapeutic options,ultimately leading to right heart failure and death.Recent findings indicate the role of the Warburg effect(aerobic glycolysis)in the development of PH.However,the effect of the glycolysis inhibitor 3-bromopyruvate(3-BrPA)on the pathogenesis of PH has not been well investigated.This study aimed to determine whether 3-BrPA inhibits PH and its possible mechanism.Methods:PH was induced in adult Sprague-Dawley rats by a single intraperitoneal injection of monocrotaline(MCT).3-BrPA,or phosphate-buffered saline(PBS)was administered via intraperitoneal injection every other day from the first day of MCT-injection to 4 weeks of follow-up,and indices such as right ventricular systolic pressure(RVSP),right ventricular hypertrophy index(RVHI),pulmonary arteriolar remodeling indicated by percent media thickness(%MT),lactate levels and glucose consumption,were evaluated.Pulmonary arteriolar remodeling and right ventricular hypertrophy were observed in hematoxylin-eosin-stained lung sections.Western blotting,immunohistochemistry,and/or immunofluorescence analyses were used to measure the expression of relevant proteins.A cytochrome C release apoptosis assay and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling staining were used to measure cell apoptosis.Results:MCT-induced PH showed a significant increase in glucose consumption(0 vs.4 weeks:0.87±0.23 vs.2.94±0.47,P=0.0042)and lactate production(0 vs.4 weeks:4.19±0.34 vs.8.06±0.67,P=0.0004).Treatment with 3-BrPA resulted in a concomitant reduction in glucose consumption(1.10±0.35 vs.3.25±0.47,P=0.0063),lactate production(5.09±0.55 vs.8.06±0.67,P=0.0065),MCT-induced increase in RVSP(39.70±2.94 vs.58.85±2.32,P=0.0004),pulmonary vascular remodeling(%MT,43.45%±1.41%vs.63.66%±1.78%,P<0.0001),and right ventricular hypertrophy(RVHI,38.57%±2.69%vs.62.61%±1.57%,P<0.0001)when compared with those of the PBS-treated group.3-BrPA,a hexokinase 2 inhibitor,exerted its beneficial effect on PH by decreasing aerobic glycolysis and was also associated with inhibiting the expression of glucose transporter protein-1,inducing apoptosis,and suppressing inflammation.Conclusions:3-BrPA might have a potential beneficial effect on the PH treatment.
