A 48 year-old Chinese woman suffering from polyarthritis,irregular fever and trichomadesis was admitted to the hospital.A diagnosis of systemic lupus erythematosus(SLE)was made based on polyarthritis,pancytopenia,redu...A 48 year-old Chinese woman suffering from polyarthritis,irregular fever and trichomadesis was admitted to the hospital.A diagnosis of systemic lupus erythematosus(SLE)was made based on polyarthritis,pancytopenia,reduced complement 3,multiple positive autoantibodies,a positive Coomb’s test and protein in her urine.In addition,splenomegaly was detected during physical examination and confirmed by abdominal ultrasonography and magnetic resonance imaging,indicating that the patient had SLE and portal hypertension.Further negative investigations ruled out the possibility of cirrhosis.The patient was diagnosed with active SLE complicated by noncirrhotic portal hypertension(NCPH)without liver histopathology,due to the patient’s refusal for liver biopsy.Portal vein diameter and splenomegaly decreased following treatment with methylprednisolone,hydroxychloroquine and metoprolol tartrate.To date,SLE complicated by NCPH has rarely been reported,as it is under-recognized clinically as well as pathologically.Here we describe a case of SLE complicated by NCPH and review the literature for its characteristics,which may contribute to improving the recognition of NCPH and reducing missed and delayed diagnosis of this disorder.展开更多
Background:Circular RNA(circRNA)is a type of closed circular noncoding RNA(ncRNA),mostly formed by back-splicing or alternative splicing of pre-messenger RNA(mRNA).The aim of this study was to explore the expression p...Background:Circular RNA(circRNA)is a type of closed circular noncoding RNA(ncRNA),mostly formed by back-splicing or alternative splicing of pre-messenger RNA(mRNA).The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells(PBMCs)of patients with ankylosing spondylitis(AS)and discover potential molecular markers of AS.Methods:The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls(HC).To screen the differentially expressed circRNAs by fold change(FC)andP value,these differentially expressed circRNAs were analyzed by bioinformatics.In 60 cases of AS and 30 cases of HC,4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction(RT-qPCR),and their correlation with various clinical indicators was analyzed.Finally,the receiver operating characteristic(ROC)curve was used to analyze their potential as AS diagnostic markers.Results:The microarray results showed that there were 1369 significantly differently expressed(P<0.05,FC>1.5)circRNAs between the AS and HC groups(675 upregulated and 694 downregulated).The results of bioinformatics analysis suggested that they were mainly involved in"enzyme binding,""adenosine ribonucleotide binding,""MAPK signaling pathway",etc.The RT-qPCR results showed that the expressions of hsacircRNA001544(U=486.5,P<0.05)and hsacircRNA102532(U=645,P<0.05)were significantly different between the AS group and the HC group.The AS group was further divided into two subgroups:active AS(ASA)and stable AS(ASS).After analysis,it was found that compared with the HC group,hsacircRNA001544 was significantly increased in both ASA(U=214,P<0.05)and ASS groups(U=273,P<0.05),while hsacircRNA008961(U=250,P<0.05)and hsacircRNA102532(U=295,P<0.05)were only significantly increased in the ASA group.Furthermore,hsacircRNA012732 was significantly different between the ASA and ASS groups(U=194,P<0.05),and there was no statistical significance among the remaining groups.Correlation analysis results showed that hsacircRNA012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index(BASDAI),high-sensitivity C-reactive protein(hsCRP),and globulin(GLOB)and positively correlated with lymphocyte count(LY),mean corpusular volume,and albumin(ALB),and hsacircRNA008961 was negatively correlated with platelet(PLT)count.ROC curve analysis showed that hsacircRNA001544(95%CI=0.610-0.831,P<0.05)and hsacircRNA102532(95%CI=0.521-0.762,P<0.05)were statistically significant,and their area under curve(AUC)values were 0.720 and 0.642,respectively.Conclusions:There are differentially expressed circRNAs in PBMCs of AS patients,and they may be involved in the occurrence and development of AS.Among these differentially expressed circRNAs,hsacircRNA012732 has the potential to become an indicator of disease activity,and hsacircRNA001544 has the potential to become a molecular marker for AS diagnosis.展开更多
基金Supported by the National Natural Science Foundation of China,No.81670801Medical Association of Sichuan Province,No.S16027+1 种基金Health and Family Planning Commission of Sichuan Province,No.17PJ059Science and Technology Department of Sichuan Province,No.2018JY0498
文摘A 48 year-old Chinese woman suffering from polyarthritis,irregular fever and trichomadesis was admitted to the hospital.A diagnosis of systemic lupus erythematosus(SLE)was made based on polyarthritis,pancytopenia,reduced complement 3,multiple positive autoantibodies,a positive Coomb’s test and protein in her urine.In addition,splenomegaly was detected during physical examination and confirmed by abdominal ultrasonography and magnetic resonance imaging,indicating that the patient had SLE and portal hypertension.Further negative investigations ruled out the possibility of cirrhosis.The patient was diagnosed with active SLE complicated by noncirrhotic portal hypertension(NCPH)without liver histopathology,due to the patient’s refusal for liver biopsy.Portal vein diameter and splenomegaly decreased following treatment with methylprednisolone,hydroxychloroquine and metoprolol tartrate.To date,SLE complicated by NCPH has rarely been reported,as it is under-recognized clinically as well as pathologically.Here we describe a case of SLE complicated by NCPH and review the literature for its characteristics,which may contribute to improving the recognition of NCPH and reducing missed and delayed diagnosis of this disorder.
基金supported by grants from the National Natural Science Foundation of China(No.81974250)Nanchong City Science and Technology Project(No.18SXHZ0522)。
文摘Background:Circular RNA(circRNA)is a type of closed circular noncoding RNA(ncRNA),mostly formed by back-splicing or alternative splicing of pre-messenger RNA(mRNA).The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells(PBMCs)of patients with ankylosing spondylitis(AS)and discover potential molecular markers of AS.Methods:The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls(HC).To screen the differentially expressed circRNAs by fold change(FC)andP value,these differentially expressed circRNAs were analyzed by bioinformatics.In 60 cases of AS and 30 cases of HC,4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction(RT-qPCR),and their correlation with various clinical indicators was analyzed.Finally,the receiver operating characteristic(ROC)curve was used to analyze their potential as AS diagnostic markers.Results:The microarray results showed that there were 1369 significantly differently expressed(P<0.05,FC>1.5)circRNAs between the AS and HC groups(675 upregulated and 694 downregulated).The results of bioinformatics analysis suggested that they were mainly involved in"enzyme binding,""adenosine ribonucleotide binding,""MAPK signaling pathway",etc.The RT-qPCR results showed that the expressions of hsacircRNA001544(U=486.5,P<0.05)and hsacircRNA102532(U=645,P<0.05)were significantly different between the AS group and the HC group.The AS group was further divided into two subgroups:active AS(ASA)and stable AS(ASS).After analysis,it was found that compared with the HC group,hsacircRNA001544 was significantly increased in both ASA(U=214,P<0.05)and ASS groups(U=273,P<0.05),while hsacircRNA008961(U=250,P<0.05)and hsacircRNA102532(U=295,P<0.05)were only significantly increased in the ASA group.Furthermore,hsacircRNA012732 was significantly different between the ASA and ASS groups(U=194,P<0.05),and there was no statistical significance among the remaining groups.Correlation analysis results showed that hsacircRNA012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index(BASDAI),high-sensitivity C-reactive protein(hsCRP),and globulin(GLOB)and positively correlated with lymphocyte count(LY),mean corpusular volume,and albumin(ALB),and hsacircRNA008961 was negatively correlated with platelet(PLT)count.ROC curve analysis showed that hsacircRNA001544(95%CI=0.610-0.831,P<0.05)and hsacircRNA102532(95%CI=0.521-0.762,P<0.05)were statistically significant,and their area under curve(AUC)values were 0.720 and 0.642,respectively.Conclusions:There are differentially expressed circRNAs in PBMCs of AS patients,and they may be involved in the occurrence and development of AS.Among these differentially expressed circRNAs,hsacircRNA012732 has the potential to become an indicator of disease activity,and hsacircRNA001544 has the potential to become a molecular marker for AS diagnosis.
