OBJECTIVE: To investigate the effect of Young Yum pill (YYP) on inflammatory mediators in cultured RAW 264.7 cells and elucidate the nuclear factor- kappa B (NF-κB)-related mechanism behind the action. METHODS: YYP w...OBJECTIVE: To investigate the effect of Young Yum pill (YYP) on inflammatory mediators in cultured RAW 264.7 cells and elucidate the nuclear factor- kappa B (NF-κB)-related mechanism behind the action. METHODS: YYP was extracted with 95% ethanol Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages were used to evaluate the effect of YYP on inflammatory mediators. Production of nitric oxide (NO) and prostaglandin E2 (PGE2) were measured by Griess test and enzyme-linked immunosorbent assay, respectively. The levels of genes and proteins involved in the generation of inflammatory mediators were examined using real-time polymerase chain reaction and Western blotting, respectively. RESULTS: YYP dose-dependently suppressed LPS-induced production of NO, PGE2 and tumor necrosis factor-α(TNF-α), and elevation of mRNA and protein levels of inducible NO synthase and cyclooxygenase- 2 in RAW 264.7 macrophages. These observations were associated with decreased NF-κB p65 phosphorylation and nuclear localization, enhanced Akt (protein kinase B) phosphorylation, as well as reduced inhibitor of κB (IκB)α degradation and IκB kinase α/β phosphorylation. CONCLUSION: The present study demonstrated an inhibitory effect of YYP on the NF-κB-regulated inflammatory mediators NO, PGE2 and TNF-α in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for the use of YYP in treating inflammatory disorders.展开更多
目的分析国外青少年怀孕社会背景,探讨国外青少年怀孕干预政策,为我国制定青少年怀孕干预政策提供循证依据和政策建议。方法计算机检索Cochrane Library、PubMed、Web of Science、Embase等数据库建库至2023年4月收录的青少年怀孕相关文...目的分析国外青少年怀孕社会背景,探讨国外青少年怀孕干预政策,为我国制定青少年怀孕干预政策提供循证依据和政策建议。方法计算机检索Cochrane Library、PubMed、Web of Science、Embase等数据库建库至2023年4月收录的青少年怀孕相关文献,分析各国有关青少年怀孕预防采取的措施及政策和法律。结果美国、哥斯达黎加、墨西哥等国家在青少年怀孕干预方面有项目、政策和立法的实施,其干预项目主要分为4个方向:父母干预、学校干预、社区干预、医疗卫生干预;干预政策及法律聚焦于保护青少年权利,并通过提供教育及性与生殖健康相关服务来确保青少年健康。结论发达国家应关注如何提供高接受度的服务以减少青少年怀孕;而对于难以满足青少年保健服务的国家,应保障基本医疗和社会服务,降低青少年怀孕风险;发展中国家则需健全青少年怀孕预防体系,各部门协作营造友好的环境,提供可及性服务。展开更多
OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effect...OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction analysis.Anti-CRC compounds were quantified by high performance liquid chromatography.RESULTS:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,respectively.BLE-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.CONCLUSION:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin signaling.Further studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.展开更多
基金Supported by Wai Yuen Tong Medicine Company Limited,Innovation and Technology Fund(UIT/315)General Research Fund(GRF:12125116)of Hong KongFRG1/16-17/048 and FRG2/16-17/033 from the Hong Kong Baptist University
文摘OBJECTIVE: To investigate the effect of Young Yum pill (YYP) on inflammatory mediators in cultured RAW 264.7 cells and elucidate the nuclear factor- kappa B (NF-κB)-related mechanism behind the action. METHODS: YYP was extracted with 95% ethanol Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages were used to evaluate the effect of YYP on inflammatory mediators. Production of nitric oxide (NO) and prostaglandin E2 (PGE2) were measured by Griess test and enzyme-linked immunosorbent assay, respectively. The levels of genes and proteins involved in the generation of inflammatory mediators were examined using real-time polymerase chain reaction and Western blotting, respectively. RESULTS: YYP dose-dependently suppressed LPS-induced production of NO, PGE2 and tumor necrosis factor-α(TNF-α), and elevation of mRNA and protein levels of inducible NO synthase and cyclooxygenase- 2 in RAW 264.7 macrophages. These observations were associated with decreased NF-κB p65 phosphorylation and nuclear localization, enhanced Akt (protein kinase B) phosphorylation, as well as reduced inhibitor of κB (IκB)α degradation and IκB kinase α/β phosphorylation. CONCLUSION: The present study demonstrated an inhibitory effect of YYP on the NF-κB-regulated inflammatory mediators NO, PGE2 and TNF-α in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for the use of YYP in treating inflammatory disorders.
文摘目的分析国外青少年怀孕社会背景,探讨国外青少年怀孕干预政策,为我国制定青少年怀孕干预政策提供循证依据和政策建议。方法计算机检索Cochrane Library、PubMed、Web of Science、Embase等数据库建库至2023年4月收录的青少年怀孕相关文献,分析各国有关青少年怀孕预防采取的措施及政策和法律。结果美国、哥斯达黎加、墨西哥等国家在青少年怀孕干预方面有项目、政策和立法的实施,其干预项目主要分为4个方向:父母干预、学校干预、社区干预、医疗卫生干预;干预政策及法律聚焦于保护青少年权利,并通过提供教育及性与生殖健康相关服务来确保青少年健康。结论发达国家应关注如何提供高接受度的服务以减少青少年怀孕;而对于难以满足青少年保健服务的国家,应保障基本医疗和社会服务,降低青少年怀孕风险;发展中国家则需健全青少年怀孕预防体系,各部门协作营造友好的环境,提供可及性服务。
基金Supported by Food and Health Bureau of Hong Kong:Elucidating the Involvement of IL-17-IL-6-STAT3 Axis in the anti-melanoma Effects of a Herbal Formula Comprising Flos Sophorae and Flos Lonicerae(No.HMRF14150571)Science,Technology and Innovation Commission of Shenzhen:anti-colorectal Cancer Effects of Bailian(Radix Ampelopsis Japonicae)extract(No.JCYJ20140807091945050)+3 种基金Exploring the Mechanisms for the anti-rheumatoid Rrthritis Effects of Chinese Medicinal Herb Based on Targeting the TLR4 Signaling Pathway(No.JCYJ20160229210327924)The Research Grants Council of Hong Kong:Elucidating the role of TLR4/STAT3 Signaling in the Antimelanoma Effects of Atractylenolide Ⅱ(No.12125116)The National Natural Science Foundation of China:Exploring the let-7/IGF1R-related Mechanism of Action for the Anti-melanoma Effects of a Herbal formula Huai Hua Jin Yin Jiu(No.81673649)Guangdong Natural Science Foundation:Evaluating the Involvement of miR-34b in the Anti-melanoma Action of Si-Jun-Zi-Tang(No.2016A030313007)
文摘OBJECTIVE:To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae,BL) extract action on colorectal cancer (CRC).METHODS:We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,immunofluorescent staining,luciferase assay,Western blot analysis and real-time quantitative polymerase chain reaction analysis.Anti-CRC compounds were quantified by high performance liquid chromatography.RESULTS:The contents of gallic acid,catechin,and epicatechin in the BLE were 0.23,1.25,and 0.18 g/kg,respectively.BLE-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity,reduced β-catenin nuclear localization,and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.CONCLUSION:The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin signaling.Further studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.
