Background:Recent studies have shown glycerolipid metabolism played an essential role in multiple tumors,however,its function in osteosarcoma is unclear.This study aimed to explore the role of glycerolipid metabolism ...Background:Recent studies have shown glycerolipid metabolism played an essential role in multiple tumors,however,its function in osteosarcoma is unclear.This study aimed to explore the role of glycerolipid metabolism in osteosarcoma.Methods:We conducted bioinformatics analysis using data from the Therapeutically Applicable Research to Generate Effective Treatments(TARGET)database and single-cell RNA sequencing.Least Absolute Shrinkage and Selection Operator(LASSO)regression was used to identify the Glycerolipid metabolism-related genes associated with the clinical outcome of osteosarcoma.Tumor-associated macrophages(TAMs)and their interactions with immune cells were examined through single-cell analysis and co-culture experiments.Virtual screening was employed to identify the potential lysophosphatidic acid receptor 6(LPAR6)inhibitors.Results:Glycerolipid metabolism-related genes 1-acylglycerol-3-phosphate O-acyltransferase 3(AGPAT3)and aldehyde dehydrogenase 7 family member A1(ALDH7A1)were identified as key prognostic genes in osteosarcoma,with high AGPAT3 expression correlating with improved survival.Single-cell analysis revealed that AGPAT3 expression is associated with tumor immune microenvironment,particularly with TAMs.Knockdown of AGPAT3 in osteosarcoma cells resulted in elevated lysophosphatidic acid(LPA)levels,which regulated the immune environment,inhibiting cytotoxic T cell function through TAMs’LPAR6 signaling.LPAR6 signaling in TAMs mediates immune regulation through cytokine secretion,including interleukin-6(IL-6)and interleukin-10(IL-10).Further drug virtual screening identified Dutasteride as a potential inhibitor of LPAR6.Conclusion:AGPAT3 is an important gene related to the prognosis of osteosarcoma.Its ability to modulate LPA signaling and TAM activity offers promising therapeutic opportunities for improving osteosarcoma treatment,particularly in immunotherapy contexts.展开更多
The corrosion behavior and microstructure characteristics of metal inert gas(MIG)welded dissimilar joints of the 6005A alloy modified with Sc(designated as 6005A+Sc)and the 5083 alloy were investigated using corrosion...The corrosion behavior and microstructure characteristics of metal inert gas(MIG)welded dissimilar joints of the 6005A alloy modified with Sc(designated as 6005A+Sc)and the 5083 alloy were investigated using corrosion tests and microscopy techniques.Results show that the dissimilar joints exhibit strong stress corrosion cracking(SCC)resistance,maintaining substantial strength during slow strain rate tensile tests.Notably,the heat-affected zone(HAZ)and base metal(BM)on the 6005A+Sc side show superior performance in terms of inter-granular corrosion(IGC)and exfoliation corrosion(EXCO)compared to the corresponding zones on the 5083 side.The lower corrosion resistance of the 5083-BM and the 5083-HAZ can be attributed to the presence of numerous Al_(2)Mg_(3)phases and micro-scaled Al_(6)(Mn,Fe)intermetallics,mainly distributed along the rolling direction.Conversely,the enhanced corrosion resistance of the 6005A+Sc-BM and the 6005A+Sc-HAZ can be attributed to the discontinuously distributed grain boundary precipitates(β-Mg_(2)Si),the smaller grain size,and the reduced corrosive current density.展开更多
The reduction of carbon emissions in the steel industry is a significant challenge,and utilizing CO_(2) from carbon intensive steel industry off-gases for methanol production is a promising strategy for decarbonizatio...The reduction of carbon emissions in the steel industry is a significant challenge,and utilizing CO_(2) from carbon intensive steel industry off-gases for methanol production is a promising strategy for decarbonization.However,steelwork off-gases typically contain various impurities,including H_(2)S,which can deactivate commercial methanol synthesis catalysts,Cu/ZnO/Al_(2)O_(3)(CZA).Reverse water-gas shift(RWGS)reaction is the predominant side reaction in CO_(2) hydrogenation to methanol which can occur at ambient pressure,enabling the decouple of RWGS from methanol production at high pressure.Then,a series of activated CZA catalysts has been in-situ pretreated in 400 ppm H_(2)S/Ar at 250℃and tested for both RWGS reaction at ambient pressure and CO_(2) hydrogenation to methanol at high pressure.An innovative decoupling strategy was employed to isolate the RWGS reaction from the methanol synthesis process,enabling the investigation of the evolution of active site structures and the poisoning mechanism through elemental analysis,X-ray Diffraction,X-ray Photoelectron Spectroscopy,Fourier Transform Infrared Spectroscopy,Temperature Programmed Reduction and CO_(2) Temperature Programmed Desorption.The results indicate that there are different dynamic migration behaviors of ZnO_(x) in the two reaction systems,leading to different poisoning mechanisms.These interesting findings are beneficial to develop sulfur resistant and durable highly efficient catalysts for CO_(2) hydrogenation to methanol,promoting the carbon emission reduction in steel industry.展开更多
BACKGROUND Indolent T-cell lymphoma of the gastrointestinal tract(iTCL-GI)is a rare mature T-cell lymphoma that has been formally recognized as a distinct entity in the 5th Edition World Health Organization Classifica...BACKGROUND Indolent T-cell lymphoma of the gastrointestinal tract(iTCL-GI)is a rare mature T-cell lymphoma that has been formally recognized as a distinct entity in the 5th Edition World Health Organization Classification of Tumours of Haematolymphoid Tumours.However,the coexistence of iTCL-GI with epithelial malignancies is rare in clinical practice.This study reports a case of iTCL-GI with gastric signet-ring cell carcinoma(SRCC).We aim to increase diagnostic awareness among clinicians and pathologists regarding multiple primary tumors.CASE SUMMARY A 65-year-old female presented with a 5-month history of lower abdominal pain,bloating,and vomiting.An abdominal computed tomography scan revealed irregular thickening of the gastric wall.Endoscopy revealed diffuse mucosal edema and rigid mucosa along the lesser curvature of the gastric body.There was a 1.5 cm mucosal protrusion on the greater curvature.Biopsy revealed that the lamina propria was expanded by a dense,nondestructive infiltrate of small lymphocytes in the greater curvature,which were characterized by a CD3+/CD8+/TIA-1+immunophenotype with a low Ki-67 index.Clonal T-cell receptor rearrangement was detected,but the Epstein-Barr virus encoded RNA's was negative.Lesions on the lesser curvature of the gastric body were confirmed to be SRCCs.After three months of follow-up,the patient completed four cycles of chemotherapy targeting the SRCC.While her abdominal pain improved,she experienced a weight loss of 5 kg.CONCLUSION Clinicians and pathologists must integrate assessment of these rare cases to prevent misdiagnosis and guide clinical practice.展开更多
BACKGROUND The primary complication associated with gestational diabetes mellitus(GDM)is delivery of an infant that is large for gestational age(LGA).Epidemiological findings have demonstrated that irregular lipid met...BACKGROUND The primary complication associated with gestational diabetes mellitus(GDM)is delivery of an infant that is large for gestational age(LGA).Epidemiological findings have demonstrated that irregular lipid metabolism significantly con-tributes to insulin resistance,a key pathophysiological mechanism in GDM.However,the correlation between various lipid indices and the probability of delivering LGA infants remains inconsistent.AIM To explore the relationships between lipid indices and the possibility of having LGA infants among GDM-affected pregnant females.METHODS Binary logistic regression methods were employed to evaluate the odds ratios and corresponding 95%confidence intervals for LGA according to five lipid indices.Restricted cubic spline models were applied to investigate dose-response relationships.The association between lipid indices and the risk of delivering LGA infants was further investigated among different subgroups.Receiver operating characteristic curves were utilized to assess the diagnostic performance of lipid indices.RESULTS Across crude and adjusted models,females with lipid indices in the upper two tertiles presented a markedly elevated risk of delivering LGA infants compared with the lowest tertile category.Conversely,high-density lipoprotein cholesterol levels demonstrated the contrary trend.Restricted cubic spline analyses revealed linear associations between the five lipid indices,except triglyceride levels,and the prevalence of LGA.The subgroup analysis highlighted that the correlation between lipid indices and the probability of LGA was inconsistent.The five lipid indices presented significant diagnostic efficacy,as indicated by receiver operating characteristic curve areas.CONCLUSION Our research demonstrated that lipid indices were effective predictors of the incidence of LGA infants in GDM-affected pregnancies irrespective of potential confounding factors.展开更多
Lamina structures,as typical sedimentary features in shale formations,determine both the quality of shale reservoirs and fracturing effects.In this study,through electric imaging logging,based on core scanning photos,...Lamina structures,as typical sedimentary features in shale formations,determine both the quality of shale reservoirs and fracturing effects.In this study,through electric imaging logging,based on core scanning photos,thin sections,and other data from the Wufeng-Longmaxi Formation shale reservoirs in the western Sichuan Block,the characteristics and classification scheme of deep shale gas reservoir laminaset were clarified.In addition,with core scale electrical images,the electrical imaging logging response characteristics of different types of laminaset were identified.Based on electrical imaging logging images,a laminaset clustering algorithm was designed to segment the laminaset and then Levenberg-Marquardt(L-M)algorithm was improved by introducing a random forest to obtain the R-L-M algorithm,which was used to extract key parameters of lam-inaset such as attitude,type,density,and thickness.The average accuracy,recall rate,and F1 score of laminaset recognition results of this algorithm were 14.82%higher than those of a well-known international commercial software(T).This method was used to evaluate the Longmaxi Formation shale gas reservoir in the western Sichuan Block.The development density of clay-siliceous(organic-lean)laminaset from the Longyi 1-4 small layer to the lower Wufeng Formation firstly decreased and then increased and the minimum value was found in Longyi 1-1 small layer.In contrast,the development density of siliceous-clay laminaset(organic-rich)first increased and then gradually decreased and the maximum value was found in Longyi 1-1 small layer.The clay-siliceous laminaset(organic matters-contained)and the calcareous-clay laminaset(organic matters-contained)showed a stable developmental trend.展开更多
基金supported by the National Natural Science Foundation of China(grant number 82460425)Jiangxi Provincial Health Technology Project(grant number 202510141).
文摘Background:Recent studies have shown glycerolipid metabolism played an essential role in multiple tumors,however,its function in osteosarcoma is unclear.This study aimed to explore the role of glycerolipid metabolism in osteosarcoma.Methods:We conducted bioinformatics analysis using data from the Therapeutically Applicable Research to Generate Effective Treatments(TARGET)database and single-cell RNA sequencing.Least Absolute Shrinkage and Selection Operator(LASSO)regression was used to identify the Glycerolipid metabolism-related genes associated with the clinical outcome of osteosarcoma.Tumor-associated macrophages(TAMs)and their interactions with immune cells were examined through single-cell analysis and co-culture experiments.Virtual screening was employed to identify the potential lysophosphatidic acid receptor 6(LPAR6)inhibitors.Results:Glycerolipid metabolism-related genes 1-acylglycerol-3-phosphate O-acyltransferase 3(AGPAT3)and aldehyde dehydrogenase 7 family member A1(ALDH7A1)were identified as key prognostic genes in osteosarcoma,with high AGPAT3 expression correlating with improved survival.Single-cell analysis revealed that AGPAT3 expression is associated with tumor immune microenvironment,particularly with TAMs.Knockdown of AGPAT3 in osteosarcoma cells resulted in elevated lysophosphatidic acid(LPA)levels,which regulated the immune environment,inhibiting cytotoxic T cell function through TAMs’LPAR6 signaling.LPAR6 signaling in TAMs mediates immune regulation through cytokine secretion,including interleukin-6(IL-6)and interleukin-10(IL-10).Further drug virtual screening identified Dutasteride as a potential inhibitor of LPAR6.Conclusion:AGPAT3 is an important gene related to the prognosis of osteosarcoma.Its ability to modulate LPA signaling and TAM activity offers promising therapeutic opportunities for improving osteosarcoma treatment,particularly in immunotherapy contexts.
基金financially supported by the Science and Technology Innovation Program of Hunan Province,China(No.2023RC3055)the Natural Science Foundation of Hunan Province,China(Nos.2023JJ30671,2020JJ4114)+5 种基金the Natural Science Foundation of Changsha City,China(No.Kq2208264)National Key Project of Research and Development Plan of China(Nos.2021YFC1910505,2021YFC1910504)the Young Core Teacher Foundation of Hunan Province,China(No.150220001)Key Research and Development Program of Guangdong Province,China(No.2020B010186002)the National Natural Science Foundation of China(No.51601229)the Key-Area Research and Development Program of Foshan City,China(No.2230032004640).
文摘The corrosion behavior and microstructure characteristics of metal inert gas(MIG)welded dissimilar joints of the 6005A alloy modified with Sc(designated as 6005A+Sc)and the 5083 alloy were investigated using corrosion tests and microscopy techniques.Results show that the dissimilar joints exhibit strong stress corrosion cracking(SCC)resistance,maintaining substantial strength during slow strain rate tensile tests.Notably,the heat-affected zone(HAZ)and base metal(BM)on the 6005A+Sc side show superior performance in terms of inter-granular corrosion(IGC)and exfoliation corrosion(EXCO)compared to the corresponding zones on the 5083 side.The lower corrosion resistance of the 5083-BM and the 5083-HAZ can be attributed to the presence of numerous Al_(2)Mg_(3)phases and micro-scaled Al_(6)(Mn,Fe)intermetallics,mainly distributed along the rolling direction.Conversely,the enhanced corrosion resistance of the 6005A+Sc-BM and the 6005A+Sc-HAZ can be attributed to the discontinuously distributed grain boundary precipitates(β-Mg_(2)Si),the smaller grain size,and the reduced corrosive current density.
基金supported by the National Natural Science Foundation of China(Nos.22276060 and 21976059)Guangdong Basic and Applied Basic Research Foundation(No.2024A1515012636)China Scholarship Council Scholarship(No.201906155006)。
文摘The reduction of carbon emissions in the steel industry is a significant challenge,and utilizing CO_(2) from carbon intensive steel industry off-gases for methanol production is a promising strategy for decarbonization.However,steelwork off-gases typically contain various impurities,including H_(2)S,which can deactivate commercial methanol synthesis catalysts,Cu/ZnO/Al_(2)O_(3)(CZA).Reverse water-gas shift(RWGS)reaction is the predominant side reaction in CO_(2) hydrogenation to methanol which can occur at ambient pressure,enabling the decouple of RWGS from methanol production at high pressure.Then,a series of activated CZA catalysts has been in-situ pretreated in 400 ppm H_(2)S/Ar at 250℃and tested for both RWGS reaction at ambient pressure and CO_(2) hydrogenation to methanol at high pressure.An innovative decoupling strategy was employed to isolate the RWGS reaction from the methanol synthesis process,enabling the investigation of the evolution of active site structures and the poisoning mechanism through elemental analysis,X-ray Diffraction,X-ray Photoelectron Spectroscopy,Fourier Transform Infrared Spectroscopy,Temperature Programmed Reduction and CO_(2) Temperature Programmed Desorption.The results indicate that there are different dynamic migration behaviors of ZnO_(x) in the two reaction systems,leading to different poisoning mechanisms.These interesting findings are beneficial to develop sulfur resistant and durable highly efficient catalysts for CO_(2) hydrogenation to methanol,promoting the carbon emission reduction in steel industry.
基金Supported by Key Disciplines of The Sixth Cycle of Tongji Hospital,No.ZDTS24-BLShanghai Municipal Science and Technology Commission 2021"Science and Technology Innovation Action Plan"Medical Innovation Research Project,No.21Y11908800Clinical Research Project of Tongji Hospital of Tongji University,No.ITJ(ZD)2206.
文摘BACKGROUND Indolent T-cell lymphoma of the gastrointestinal tract(iTCL-GI)is a rare mature T-cell lymphoma that has been formally recognized as a distinct entity in the 5th Edition World Health Organization Classification of Tumours of Haematolymphoid Tumours.However,the coexistence of iTCL-GI with epithelial malignancies is rare in clinical practice.This study reports a case of iTCL-GI with gastric signet-ring cell carcinoma(SRCC).We aim to increase diagnostic awareness among clinicians and pathologists regarding multiple primary tumors.CASE SUMMARY A 65-year-old female presented with a 5-month history of lower abdominal pain,bloating,and vomiting.An abdominal computed tomography scan revealed irregular thickening of the gastric wall.Endoscopy revealed diffuse mucosal edema and rigid mucosa along the lesser curvature of the gastric body.There was a 1.5 cm mucosal protrusion on the greater curvature.Biopsy revealed that the lamina propria was expanded by a dense,nondestructive infiltrate of small lymphocytes in the greater curvature,which were characterized by a CD3+/CD8+/TIA-1+immunophenotype with a low Ki-67 index.Clonal T-cell receptor rearrangement was detected,but the Epstein-Barr virus encoded RNA's was negative.Lesions on the lesser curvature of the gastric body were confirmed to be SRCCs.After three months of follow-up,the patient completed four cycles of chemotherapy targeting the SRCC.While her abdominal pain improved,she experienced a weight loss of 5 kg.CONCLUSION Clinicians and pathologists must integrate assessment of these rare cases to prevent misdiagnosis and guide clinical practice.
基金Supported by Nanjing Medical Science and Technique Development Foundation,No.YKK23151the Opening Foundation of Key Laboratory,No.JSHD202313+3 种基金Yingke Xinchuang Research Foundation of Jiangsu Blood Transfusion Association,No.JSYK2024006the Jiangsu Province Capability Improvement Project through Science,Technology and Education,No.ZDXYS202210Open Project of the State Key Laboratory of Reproductive Medicine of Nanjing Medical University,No.SKLRM-K202107the Jiangsu Provincial Maternal and Child Health Research Program,No.F202040.
文摘BACKGROUND The primary complication associated with gestational diabetes mellitus(GDM)is delivery of an infant that is large for gestational age(LGA).Epidemiological findings have demonstrated that irregular lipid metabolism significantly con-tributes to insulin resistance,a key pathophysiological mechanism in GDM.However,the correlation between various lipid indices and the probability of delivering LGA infants remains inconsistent.AIM To explore the relationships between lipid indices and the possibility of having LGA infants among GDM-affected pregnant females.METHODS Binary logistic regression methods were employed to evaluate the odds ratios and corresponding 95%confidence intervals for LGA according to five lipid indices.Restricted cubic spline models were applied to investigate dose-response relationships.The association between lipid indices and the risk of delivering LGA infants was further investigated among different subgroups.Receiver operating characteristic curves were utilized to assess the diagnostic performance of lipid indices.RESULTS Across crude and adjusted models,females with lipid indices in the upper two tertiles presented a markedly elevated risk of delivering LGA infants compared with the lowest tertile category.Conversely,high-density lipoprotein cholesterol levels demonstrated the contrary trend.Restricted cubic spline analyses revealed linear associations between the five lipid indices,except triglyceride levels,and the prevalence of LGA.The subgroup analysis highlighted that the correlation between lipid indices and the probability of LGA was inconsistent.The five lipid indices presented significant diagnostic efficacy,as indicated by receiver operating characteristic curve areas.CONCLUSION Our research demonstrated that lipid indices were effective predictors of the incidence of LGA infants in GDM-affected pregnancies irrespective of potential confounding factors.
基金supported by the technology project“Identification of Layered Patterns in Deep Shale Gas Reservoirs in Yuxi Block and Its Impact on Fracturing Effectiveness”(No.20230304-05)of Chongqing Shale Gas Exploration and Development Co.,Ltd.
文摘Lamina structures,as typical sedimentary features in shale formations,determine both the quality of shale reservoirs and fracturing effects.In this study,through electric imaging logging,based on core scanning photos,thin sections,and other data from the Wufeng-Longmaxi Formation shale reservoirs in the western Sichuan Block,the characteristics and classification scheme of deep shale gas reservoir laminaset were clarified.In addition,with core scale electrical images,the electrical imaging logging response characteristics of different types of laminaset were identified.Based on electrical imaging logging images,a laminaset clustering algorithm was designed to segment the laminaset and then Levenberg-Marquardt(L-M)algorithm was improved by introducing a random forest to obtain the R-L-M algorithm,which was used to extract key parameters of lam-inaset such as attitude,type,density,and thickness.The average accuracy,recall rate,and F1 score of laminaset recognition results of this algorithm were 14.82%higher than those of a well-known international commercial software(T).This method was used to evaluate the Longmaxi Formation shale gas reservoir in the western Sichuan Block.The development density of clay-siliceous(organic-lean)laminaset from the Longyi 1-4 small layer to the lower Wufeng Formation firstly decreased and then increased and the minimum value was found in Longyi 1-1 small layer.In contrast,the development density of siliceous-clay laminaset(organic-rich)first increased and then gradually decreased and the maximum value was found in Longyi 1-1 small layer.The clay-siliceous laminaset(organic matters-contained)and the calcareous-clay laminaset(organic matters-contained)showed a stable developmental trend.
