The QBC techmique in diagnosing vivax malaria was compared with that of the Giemsa stained thick smear under field conditions in Sifang Village.Junlian County,Sichuan Province,China.Blood samples were collected from 1...The QBC techmique in diagnosing vivax malaria was compared with that of the Giemsa stained thick smear under field conditions in Sifang Village.Junlian County,Sichuan Province,China.Blood samples were collected from 161 volunteer villagers.Each sample was examined with both the QBC and Giemsa techniques.Each stained Giemsa thick smear(GTS)was prepared by spreading 10ul blood over an appropriate area on a slide and examined for 300 oil immersion fields,and each QBC tube was observed for 5 min.before considering a sample to be negative.Results showed that 34 blood samples were positive for vivax malaria and 127 were negative by GTS,whereas,there were 32 positives and 129 negatives by QBC.Taking GTS as standard,the sensjtivityand specificity of the QBC technique were 79.41%and 96.06%respectively,and the concordancewas 92.55%.Distributions of different developmental stages of P.raraz parasites in the centrifuged QBC tubes were cbserved and recorded,and the results revealed that all stages except schizonts,could be found in the lower part of the platelet zone,or the interphase between the monocyte and theplatelet layers,especially the ring forms.展开更多
基金This Investigation receivod financial support from the UNDP/World Bank/WHO Specal Programne for Rosearch and Trainng in Tropical Discascs(TDR)UNDP/World Bank/WHO/TDR提供基金
文摘The QBC techmique in diagnosing vivax malaria was compared with that of the Giemsa stained thick smear under field conditions in Sifang Village.Junlian County,Sichuan Province,China.Blood samples were collected from 161 volunteer villagers.Each sample was examined with both the QBC and Giemsa techniques.Each stained Giemsa thick smear(GTS)was prepared by spreading 10ul blood over an appropriate area on a slide and examined for 300 oil immersion fields,and each QBC tube was observed for 5 min.before considering a sample to be negative.Results showed that 34 blood samples were positive for vivax malaria and 127 were negative by GTS,whereas,there were 32 positives and 129 negatives by QBC.Taking GTS as standard,the sensjtivityand specificity of the QBC technique were 79.41%and 96.06%respectively,and the concordancewas 92.55%.Distributions of different developmental stages of P.raraz parasites in the centrifuged QBC tubes were cbserved and recorded,and the results revealed that all stages except schizonts,could be found in the lower part of the platelet zone,or the interphase between the monocyte and theplatelet layers,especially the ring forms.
