本研究旨在探究线粒体复合物Ⅰ(ComplexⅠ,CⅠ)的核心结构蛋白NADH脱氢酶铁硫蛋白4(NADH Dehydrogenase(Ubiquinone)Fe S protein 4,NDUFS4)在涡虫成体干细胞功能维持与神经再生过程中的作用。通过克隆获得东亚三角涡虫(Dugesia sp.)ndu...本研究旨在探究线粒体复合物Ⅰ(ComplexⅠ,CⅠ)的核心结构蛋白NADH脱氢酶铁硫蛋白4(NADH Dehydrogenase(Ubiquinone)Fe S protein 4,NDUFS4)在涡虫成体干细胞功能维持与神经再生过程中的作用。通过克隆获得东亚三角涡虫(Dugesia sp.)ndufs4基因并进行序列及结构预测分析,结果显示其编码蛋白在保守结构域和关键氨基酸位点高度保守。采用RNA干扰技术持续敲降ndufs4表达,测定再生涡虫胚基(blastema)生长速率,并结合原位杂交与免疫组织化学等实验技术检测成体干细胞(Neoblasts)的增殖与分化、神经再生情况。结果表明,ndufs4敲降导致涡虫再生速率显著减慢,神经再生明显受损;同时,干细胞标记基因表达水平下降,干细胞分化进程受阻。上述结果说明,NDUFS4是维持涡虫成体干细胞群体功能及促进神经再生的必需蛋白,在生物再生中发挥重要调控功能。展开更多
Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight ...Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight in rice. QTLs were mapped using a BC4F4 population including 192 backcross inbred lines(BILs) derived from a backcross between Xiaolijing(XLJ) and recombinant inbred lines(RILs). The mapping population was planted in both Lingshui(Hainan, 2015) and Fuyang(Zhejiang, 2016), with the short-and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes(SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization.展开更多
An F2 population derived from the cross of WB01,an introgression line resistant to brown planthopper(BPH)originated from Oryza rufipogon Griff.and a susceptible indica variety 9311,was developed for genetic analysis a...An F2 population derived from the cross of WB01,an introgression line resistant to brown planthopper(BPH)originated from Oryza rufipogon Griff.and a susceptible indica variety 9311,was developed for genetic analysis and gene mapping.The population with 303 F2:3 families was genotyped by 141 simple sequence repeat(SSR)markers and used for gene mapping.Two softwares,Mapmaker/Exp 3.0 and Windows QTL Cartographer V2.0 were applied to detect QTLs.Totally,two QTLs resistant to BPH,named temporarily as bph22(t)and bph23(t),were identified to locate on chromosomes 4 and 8,individually had LOD values of 2.92 and 3.15,and explained 11.3%and 14.9%of the phenotypic variation,respectively.展开更多
目的:重症监护病房(Intensive Care Unit,ICU)经口气管插管患者因机械通气、禁食等因素易诱发口渴不适,管理不当可导致焦虑、非计划性拔管等不良事件发生。本研究旨在构建ICU经口气管插管患者口渴管理的审查指标,了解最佳证据的临床应...目的:重症监护病房(Intensive Care Unit,ICU)经口气管插管患者因机械通气、禁食等因素易诱发口渴不适,管理不当可导致焦虑、非计划性拔管等不良事件发生。本研究旨在构建ICU经口气管插管患者口渴管理的审查指标,了解最佳证据的临床应用现状,并通过系统性分析,识别和评估影响证据转化的障碍因素,以促进临床实践的改进。方法:根据已发表的ICU经口气管插管患者口渴管理的最佳证据,通过严格的文献质量评价,对汇总的26条最佳证据进行证据适用性分析并制订审查指标,在江苏省无锡市某三甲医院ICU环境下进行基线审查,以评估当前实践与最佳证据之间的差距。结果:最终对适用的17条证据制订了16个审查指标。审查结果显示:11个审查指标的执行率低于60%,其中9个执行率为0。障碍因素主要集中于护理人员开展证据转化过程中,未充分重视口渴管理,对相关知识了解不全面,科室缺乏口渴评估工具,科室没有随时可调配使用的口渴保湿材料及缺乏具体的口腔护理保湿推荐方案,科室未及时更新口渴相关的流程规范,缺乏与家属宣教、沟通口渴相关的宣教材料等。结论:ICU经口气管插管患者口渴管理的临床实践与最佳证据之间存在较大差距。临床护理人员应积极应用最佳证据,改善ICU经口气管插管患者的口渴症状。展开更多
文摘本研究旨在探究线粒体复合物Ⅰ(ComplexⅠ,CⅠ)的核心结构蛋白NADH脱氢酶铁硫蛋白4(NADH Dehydrogenase(Ubiquinone)Fe S protein 4,NDUFS4)在涡虫成体干细胞功能维持与神经再生过程中的作用。通过克隆获得东亚三角涡虫(Dugesia sp.)ndufs4基因并进行序列及结构预测分析,结果显示其编码蛋白在保守结构域和关键氨基酸位点高度保守。采用RNA干扰技术持续敲降ndufs4表达,测定再生涡虫胚基(blastema)生长速率,并结合原位杂交与免疫组织化学等实验技术检测成体干细胞(Neoblasts)的增殖与分化、神经再生情况。结果表明,ndufs4敲降导致涡虫再生速率显著减慢,神经再生明显受损;同时,干细胞标记基因表达水平下降,干细胞分化进程受阻。上述结果说明,NDUFS4是维持涡虫成体干细胞群体功能及促进神经再生的必需蛋白,在生物再生中发挥重要调控功能。
基金supported by grants from the National Key Research and Development Program of China (2018YFD0100806)the Zhejiang Provincial Natural Science Foundation of China (LY18C130008)+2 种基金the National Natural Science Foundation of China (31521064)the Major Project of the Genetically Modified and National Key Transgenic Research Projects, China (2016ZX08001-002)the Super Rice Breeding Innovation Team and Rice Heterosis Mechanism Research Innovation Team of the Chinese Academy of Agricultural Sciences Innovation Project (CAASASTIP-2013-CNRRI)
文摘Grain size is a major determinant of grain weight, which is one of the components of rice yield. The objective o this study was to identify novel, and important quantitative trait loci(QTLs) for grain size and weight in rice. QTLs were mapped using a BC4F4 population including 192 backcross inbred lines(BILs) derived from a backcross between Xiaolijing(XLJ) and recombinant inbred lines(RILs). The mapping population was planted in both Lingshui(Hainan, 2015) and Fuyang(Zhejiang, 2016), with the short-and long-day conditions, respectively. A total of 10 QTLs for grain length, four for grain width, four for the ratio of grain length to width, and 11 for grain weight were detected in at least one environment and were distributed across 11 chromosomes. The phenotypic variance explained ranged from 6.76–25.68%, 14.30–34.03%, 5.28–26.50%, and 3.01–22.87% for grain length, grain width, the ratio of grain length to width, and thousand grain weight, respectively. Using the sequential residual heterozygotes(SeqRHs) method, qGS7.1, a QTL for grain size and weight, was mapped in a 3.2-Mb interval on chromosome 7. No QTLs about grain size and weight were reported in previous studies in this region, providing a good candidate for functional analysis and breeding utilization.
基金funded by the Science and Technology Project for Agriculture in China:Conservation,Utilization and Exploration of Agricultural Wild Plant Sources(2010-2014)
文摘An F2 population derived from the cross of WB01,an introgression line resistant to brown planthopper(BPH)originated from Oryza rufipogon Griff.and a susceptible indica variety 9311,was developed for genetic analysis and gene mapping.The population with 303 F2:3 families was genotyped by 141 simple sequence repeat(SSR)markers and used for gene mapping.Two softwares,Mapmaker/Exp 3.0 and Windows QTL Cartographer V2.0 were applied to detect QTLs.Totally,two QTLs resistant to BPH,named temporarily as bph22(t)and bph23(t),were identified to locate on chromosomes 4 and 8,individually had LOD values of 2.92 and 3.15,and explained 11.3%and 14.9%of the phenotypic variation,respectively.
文摘目的:重症监护病房(Intensive Care Unit,ICU)经口气管插管患者因机械通气、禁食等因素易诱发口渴不适,管理不当可导致焦虑、非计划性拔管等不良事件发生。本研究旨在构建ICU经口气管插管患者口渴管理的审查指标,了解最佳证据的临床应用现状,并通过系统性分析,识别和评估影响证据转化的障碍因素,以促进临床实践的改进。方法:根据已发表的ICU经口气管插管患者口渴管理的最佳证据,通过严格的文献质量评价,对汇总的26条最佳证据进行证据适用性分析并制订审查指标,在江苏省无锡市某三甲医院ICU环境下进行基线审查,以评估当前实践与最佳证据之间的差距。结果:最终对适用的17条证据制订了16个审查指标。审查结果显示:11个审查指标的执行率低于60%,其中9个执行率为0。障碍因素主要集中于护理人员开展证据转化过程中,未充分重视口渴管理,对相关知识了解不全面,科室缺乏口渴评估工具,科室没有随时可调配使用的口渴保湿材料及缺乏具体的口腔护理保湿推荐方案,科室未及时更新口渴相关的流程规范,缺乏与家属宣教、沟通口渴相关的宣教材料等。结论:ICU经口气管插管患者口渴管理的临床实践与最佳证据之间存在较大差距。临床护理人员应积极应用最佳证据,改善ICU经口气管插管患者的口渴症状。
文摘优化模型驱动的移动边缘计算(Mobile Edge Computing,MEC)网络任务卸载与迁移策略研究基于物联网设备激增和5G技术推广的背景展开。MEC通过将计算资源迁移至网络边缘,显著降低数据传输延迟和云端压力。为此,提出一系列任务卸载与迁移策略,并通过性能评估验证其效果。实验结果表明,所提策略在典型应用场景中显著优化了关键性能指标:延迟降低约25%,能耗减少30%,任务吞吐量提升20%。具体优化包括:动态资源调度实现负载均衡,改进卸载效率;基于QoS(Qua-lity of Service)保障的迁移机制确保服务稳定性;跨层优化设计提升多任务协作能力。此外,通过机器学习预测技术,动态适应网络波动,提高系统灵活性。研究结论指出,优化模型在确保资源高效分配和任务实时性方面具备突出优势,提升了MEC网络的服务质量和用户体验。策略可广泛适用于异构网络和动态环境,具备进一步拓展的潜力。
