Sequencing technology has developed rapidly in recent years. Complete or nearly complete mitochondrial genomes(mitogenomes) of 155 species from 47 families in Heteroptera have been sequenced. However, the amounts of m...Sequencing technology has developed rapidly in recent years. Complete or nearly complete mitochondrial genomes(mitogenomes) of 155 species from 47 families in Heteroptera have been sequenced. However, the amounts of mitogenomes between those families are unbalanced, which makes it difficult to correctly discern the patterns of mitogenome rearrangement in Heteroptera. Among 21 species from ten families, ten variations in mitogenome rearrangement had been previously reported, among which the translocation between tRNA-Thr and tRNA-Pro was considered as a synapomorphy of Pyrrhocoroidea based on two mitogenomes. As only one mitogenome in each of Largidae and Pyrrhocoridae had been sequenced to conclude the synapomorphy, more mitogenomes of Pyrrhocoroidea need to be explored. In this study, additional two mitogenomes of Pyrrhocoroidea(Macrocheraia grandis grandis(Gray, 1832) and Myrmoplasta mira Gerst-cker, 1892) were sequenced. Both of them also possess the same translocation between tRNA-Thr and tRNA-Pro, which reaffirms that this kind of rearrangement is a molecular synapomorphy of Pyrrhocoroidea. Moreover, we discovered a more complex rearrangement in Myrmoplasta mira, in which six nearly identical duplications of tRNA-Thr were found located downstream of tRNA-Pro. Considering the high biodiversity of Heteroptera, more mitogenomic studies are needed to improve our knowledge about mitogenome rearrangements and the potential synapomorphies.展开更多
Microbiology is an important basic course in life science,which contains many ideological and political elements.In this paper,a teaching case was designed with"Nutrients Microorganisms and Their Functions"a...Microbiology is an important basic course in life science,which contains many ideological and political elements.In this paper,a teaching case was designed with"Nutrients Microorganisms and Their Functions"as the content to explore curriculum ideological and political elements in the course.According to the classroom teaching objectives of this class,this paper combed the professional knowledge points and explored ideological and political elements with the theme of"enhancing ecological connotation and feelings for agriculture,rural areas and farmers".The depth and breadth of teaching content were expanded by extending knowledge points and typical characters deeds,and ideological and political elements were integrated into classroom teaching by various teaching methods.The implementation of the case has improved teachers awareness and ability of ideological and political education in professional courses,and improved the level of ideological and political education construction in Microbiology.The ideological and political materials in the case can be used as a reference for other teachers.展开更多
The black rockfish (Sebastes schlegelii) is a marine species that is economically important in aquaculture, and the efficiency of its spermatogenesis is vital for its success in the aquaculture industry. Spermatogonia...The black rockfish (Sebastes schlegelii) is a marine species that is economically important in aquaculture, and the efficiency of its spermatogenesis is vital for its success in the aquaculture industry. Spermatogonia serve as the foundation of spermatogenesis in fish, possessing the ability for continuous self-renewal and progressive differentiation into mature spermatozoa. Moreover, Sertoli cells are crucial in modulating the proliferation and differentiation of spermatogonia. This study focused on the regulation of glycolysis by PLIN2a in Sertoli cells of the black rockfish and examined how the inhibition of glycolysis in these cells impacted the proliferation and differentiation processes of spermatogonia. We found that effective regulation of glycolysis was crucial for the metabolic activity and functional maintenance of Sertoli cells in black rockfish. Overexpression of plin2a in vitro enhanced glycolysis in Sertoli cells, whereas inhibition of glycolysis impaired their normal metabolic activity. In vivo inhibition of glycolysis in black rockfish testes lead to apoptosis of Sertoli cells and significantly suppressed the proliferation and differentiation of spermatogonia. These results underscore the essential role of glycolysis in the development and metabolic activity of Sertoli cells and highlight the critical regulatory role of glycolysis in determining the fate of spermatogonia. This study emphasizes the importance of regulating energy metabolism pathways, particularly glycolysis, in Sertoli cells to indirectly influence the development of spermatogonia, offering significant insights into the reproductive mechanisms of black rockfish and other teleost species.展开更多
Objective： Combined small cell lung cancer （C-SCLC） is an uncommon subgroup of small cell lung cancer （SCLC） and few clinical data can be referred. Our study is to investigate the clinical features and prognostic...Objective： Combined small cell lung cancer （C-SCLC） is an uncommon subgroup of small cell lung cancer （SCLC） and few clinical data can be referred. Our study is to investigate the clinical features and prognostic factors of C-SCLC, as well as the role of multimodality treatment.Methods： Between January 2004 and December 2012, patients with histologically diagnosed C-SCLC were retrospectively analyzed. The survivals were evaluated with the Kaplan-Meier method. Univariate and multivariate analyses were used to evaluate potential prognostic factors.Results： One hundred and fourteen patients were enrolled, with a median age of 59 （range： 20-79） years old. The most common combined component was squamous cell carcinoma （52.6%）. Among these patients, the disease was stage I, II, III and IV in 9.6%, 19.3%, 46.5% and 24.6% of the patients, respectively. Eighty patients （70.2%） received at least two of the three modalities containing chemotherapy, radiotherapy and surgery. The median follow-up was 32.5 months. The median time of overall survival （OS） was 26.2 months. On univariate analysis, smoking （P=0.029）, Karnofsky performance score （KPS） 〈80 （P=0.000）, advanced TNM stage （P=0.000）, no surgery （P=0.010）, positive resection margin （P=0.000）, positive lymph nodes ≥4 （P=0.000）, positive lymph node ratio 〉10% （P=0.000） and non-multimodality treatment （P=0.004） were associated with poor OS. Multivariate analysis confirmed that smoking, advanced TNM stage, positive resection margin and positive lymph nodes ratio 〉 10% were poor prognostic features. Conclusions： C-SCLC has a relatively early stage and good prognosis, which may due to the underestimated diagnosis in non-surgical patients. Multimodality therapy is recommended, especially for limited disease. Smoking, advanced TNM stage, positive resection margin and positive lymph nodes ratio 〉10% are poor prognostic factors.展开更多
With the deep advancement of modern educational informatization,the micro-video teaching model has gradually become an effective approach for promoting the innovation and reform of experimental teaching,owing to its a...With the deep advancement of modern educational informatization,the micro-video teaching model has gradually become an effective approach for promoting the innovation and reform of experimental teaching,owing to its advantages such as intuitive visualization,repeatability,and flexible learning.This paper addressed the limitations of the traditional zoology experiment teaching model,which include insufficiently clear teacher demonstrations,limited class time,and the difficulty of accommodating individual student differences.Accordingly,we systematically analyzed the main characteristics,implementation models,and effectiveness of the micro-video teaching model in the Zoology Experiment course.We also discussed the primary challenges encountered during its teaching practice and proposed corresponding recommendations for improvement.This analysis aimed to provide a theoretical reference for the teaching reform of Zoology Experiment in normal universities.展开更多
China exhibits a high incidence of esophageal cancer.In the context of rapid population aging,esophageal cancer has become a significant threat to the health of Chinese elderly population.Currently,there is a lack of ...China exhibits a high incidence of esophageal cancer.In the context of rapid population aging,esophageal cancer has become a significant threat to the health of Chinese elderly population.Currently,there is a lack of a clear definition or assessment method for esophageal cancer in elderly patients,and this population is frequently excluded in clinical trials.Consequently,high-level evidence and global consensus guidelines for the treatment of this population are unavailable.Since elderly patients with esophageal cancer cannot tolerate surgery and intensive chemotherapy,radiotherapy plays a vital role in their treatment.Based on recent research data on esophageal cancer in elderly patients,the expert consensus on radiotherapy for esophageal cancer in elderly patients in China was jointly developed by experts from the following organizations:Radiation Oncology Physician Branch,Chinese Medical Doctor Association;Radiation Oncology Branch,Chinese Medical Association;Professional Committee of Radiation Oncology,China Anti-Cancer Association;Expert Committee of Esophageal Cancer,Chinese Cancer Radiotherapy Alliance;Radiotherapy Sub-Committee,Professional Committee of Elderly Oncology,Gerontological Society of China.This consensus defines esophageal cancer in elderly patients,emphasizes the importance of nutritional assessment and intervention,and elaborates on the mode of radiotherapy alone and radiotherapy-based combined modality therapy mode.Moreover,this consensus organizes radiotherapy technology,radiation dose,and safety management,aiming to provide a foundational guide for clinical practice targeting esophageal cancer in elderly patients.展开更多
基金financially supported by the Ministry of Culture of the Czech Republic(DKRVO 2019/5.I.a,National Museum,00023272)
文摘Sequencing technology has developed rapidly in recent years. Complete or nearly complete mitochondrial genomes(mitogenomes) of 155 species from 47 families in Heteroptera have been sequenced. However, the amounts of mitogenomes between those families are unbalanced, which makes it difficult to correctly discern the patterns of mitogenome rearrangement in Heteroptera. Among 21 species from ten families, ten variations in mitogenome rearrangement had been previously reported, among which the translocation between tRNA-Thr and tRNA-Pro was considered as a synapomorphy of Pyrrhocoroidea based on two mitogenomes. As only one mitogenome in each of Largidae and Pyrrhocoridae had been sequenced to conclude the synapomorphy, more mitogenomes of Pyrrhocoroidea need to be explored. In this study, additional two mitogenomes of Pyrrhocoroidea(Macrocheraia grandis grandis(Gray, 1832) and Myrmoplasta mira Gerst-cker, 1892) were sequenced. Both of them also possess the same translocation between tRNA-Thr and tRNA-Pro, which reaffirms that this kind of rearrangement is a molecular synapomorphy of Pyrrhocoroidea. Moreover, we discovered a more complex rearrangement in Myrmoplasta mira, in which six nearly identical duplications of tRNA-Thr were found located downstream of tRNA-Pro. Considering the high biodiversity of Heteroptera, more mitogenomic studies are needed to improve our knowledge about mitogenome rearrangements and the potential synapomorphies.
基金Supported by Zhaoqing Science and Technology Innovation Guidance Project(202304038001)The Third Batch of Innovative Scientific Research Teams in Zhaoqing University(05),Quality Engineering and Teaching Reform Project of Zhaoqing University(zlgc202261,zlgc2024082,zlgc202229)Curriculum Ideological and Political Reform Demonstration Project of Zhaoqing University(model class serial number 13,model course serial number 2).
文摘Microbiology is an important basic course in life science,which contains many ideological and political elements.In this paper,a teaching case was designed with"Nutrients Microorganisms and Their Functions"as the content to explore curriculum ideological and political elements in the course.According to the classroom teaching objectives of this class,this paper combed the professional knowledge points and explored ideological and political elements with the theme of"enhancing ecological connotation and feelings for agriculture,rural areas and farmers".The depth and breadth of teaching content were expanded by extending knowledge points and typical characters deeds,and ideological and political elements were integrated into classroom teaching by various teaching methods.The implementation of the case has improved teachers awareness and ability of ideological and political education in professional courses,and improved the level of ideological and political education construction in Microbiology.The ideological and political materials in the case can be used as a reference for other teachers.
基金supported by the National Natural Science Foundation of China[No.32273133 and No.31970492]High-performance Computing Platform of YZBSTCACC.
文摘The black rockfish (Sebastes schlegelii) is a marine species that is economically important in aquaculture, and the efficiency of its spermatogenesis is vital for its success in the aquaculture industry. Spermatogonia serve as the foundation of spermatogenesis in fish, possessing the ability for continuous self-renewal and progressive differentiation into mature spermatozoa. Moreover, Sertoli cells are crucial in modulating the proliferation and differentiation of spermatogonia. This study focused on the regulation of glycolysis by PLIN2a in Sertoli cells of the black rockfish and examined how the inhibition of glycolysis in these cells impacted the proliferation and differentiation processes of spermatogonia. We found that effective regulation of glycolysis was crucial for the metabolic activity and functional maintenance of Sertoli cells in black rockfish. Overexpression of plin2a in vitro enhanced glycolysis in Sertoli cells, whereas inhibition of glycolysis impaired their normal metabolic activity. In vivo inhibition of glycolysis in black rockfish testes lead to apoptosis of Sertoli cells and significantly suppressed the proliferation and differentiation of spermatogonia. These results underscore the essential role of glycolysis in the development and metabolic activity of Sertoli cells and highlight the critical regulatory role of glycolysis in determining the fate of spermatogonia. This study emphasizes the importance of regulating energy metabolism pathways, particularly glycolysis, in Sertoli cells to indirectly influence the development of spermatogonia, offering significant insights into the reproductive mechanisms of black rockfish and other teleost species.
基金supported by the Capital Health Development Research Grant for Youth Scholars (20114002-05)the Funding for Talents Training Project in Beijing (2012D009008000001)
文摘Objective： Combined small cell lung cancer （C-SCLC） is an uncommon subgroup of small cell lung cancer （SCLC） and few clinical data can be referred. Our study is to investigate the clinical features and prognostic factors of C-SCLC, as well as the role of multimodality treatment.Methods： Between January 2004 and December 2012, patients with histologically diagnosed C-SCLC were retrospectively analyzed. The survivals were evaluated with the Kaplan-Meier method. Univariate and multivariate analyses were used to evaluate potential prognostic factors.Results： One hundred and fourteen patients were enrolled, with a median age of 59 （range： 20-79） years old. The most common combined component was squamous cell carcinoma （52.6%）. Among these patients, the disease was stage I, II, III and IV in 9.6%, 19.3%, 46.5% and 24.6% of the patients, respectively. Eighty patients （70.2%） received at least two of the three modalities containing chemotherapy, radiotherapy and surgery. The median follow-up was 32.5 months. The median time of overall survival （OS） was 26.2 months. On univariate analysis, smoking （P=0.029）, Karnofsky performance score （KPS） 〈80 （P=0.000）, advanced TNM stage （P=0.000）, no surgery （P=0.010）, positive resection margin （P=0.000）, positive lymph nodes ≥4 （P=0.000）, positive lymph node ratio 〉10% （P=0.000） and non-multimodality treatment （P=0.004） were associated with poor OS. Multivariate analysis confirmed that smoking, advanced TNM stage, positive resection margin and positive lymph nodes ratio 〉 10% were poor prognostic features. Conclusions： C-SCLC has a relatively early stage and good prognosis, which may due to the underestimated diagnosis in non-surgical patients. Multimodality therapy is recommended, especially for limited disease. Smoking, advanced TNM stage, positive resection margin and positive lymph nodes ratio 〉10% are poor prognostic factors.
基金Supported by Undergraduate Higher Education Teaching Quality and Reform Projects of Guangdong Province(Yuejiao Gao Han[2024]No.9)Yuejiao Gao Han[2024]No.30)+5 种基金Curriculum Ideological and Political Reform Demonstration Project of Zhaoqing University(Zhao Xue Yuan[2024]No.83)Key Research Project of Zhaoqing University(ZD202407)Quality Engineering and Teaching Reform Projects of Zhaoqing University(zlgc202207zlgc2024005zlgc202239zlgc2024038).
文摘With the deep advancement of modern educational informatization,the micro-video teaching model has gradually become an effective approach for promoting the innovation and reform of experimental teaching,owing to its advantages such as intuitive visualization,repeatability,and flexible learning.This paper addressed the limitations of the traditional zoology experiment teaching model,which include insufficiently clear teacher demonstrations,limited class time,and the difficulty of accommodating individual student differences.Accordingly,we systematically analyzed the main characteristics,implementation models,and effectiveness of the micro-video teaching model in the Zoology Experiment course.We also discussed the primary challenges encountered during its teaching practice and proposed corresponding recommendations for improvement.This analysis aimed to provide a theoretical reference for the teaching reform of Zoology Experiment in normal universities.
文摘China exhibits a high incidence of esophageal cancer.In the context of rapid population aging,esophageal cancer has become a significant threat to the health of Chinese elderly population.Currently,there is a lack of a clear definition or assessment method for esophageal cancer in elderly patients,and this population is frequently excluded in clinical trials.Consequently,high-level evidence and global consensus guidelines for the treatment of this population are unavailable.Since elderly patients with esophageal cancer cannot tolerate surgery and intensive chemotherapy,radiotherapy plays a vital role in their treatment.Based on recent research data on esophageal cancer in elderly patients,the expert consensus on radiotherapy for esophageal cancer in elderly patients in China was jointly developed by experts from the following organizations:Radiation Oncology Physician Branch,Chinese Medical Doctor Association;Radiation Oncology Branch,Chinese Medical Association;Professional Committee of Radiation Oncology,China Anti-Cancer Association;Expert Committee of Esophageal Cancer,Chinese Cancer Radiotherapy Alliance;Radiotherapy Sub-Committee,Professional Committee of Elderly Oncology,Gerontological Society of China.This consensus defines esophageal cancer in elderly patients,emphasizes the importance of nutritional assessment and intervention,and elaborates on the mode of radiotherapy alone and radiotherapy-based combined modality therapy mode.Moreover,this consensus organizes radiotherapy technology,radiation dose,and safety management,aiming to provide a foundational guide for clinical practice targeting esophageal cancer in elderly patients.
