Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of dep...Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of depression by exposing mice that had previously received chronic stress to intraperitoneal LPS injections.The mice were divided into the following groups:control,model,fluoxetine,Tiansi Yin,Sini powder,and low-,medium-,and high-dose Peiyuan Jieyu formula groups.Forced swim and tail suspension tests were used to assess the efficacy of the depression(despair)model,and weight gain rates were also measured.Furthermore,serum levels of various depression and inflammation-associated molecules,including tumor necrosis factor-a(TNF-a),interferon-γ(IFN-γ),tryptophan,5-hydroxytryptamine,kynurenine(KYN),and kynurenic acid(KA)were assessed.Furthermore,the expression levels of ionic calcium-binding adaptor molecule-1(IBA-1)and indoleamine 2,3-dioxygenase(IDO)mRNA in hippocampal microglia were measured.Results:The model group displayed greater despair-associated immobility,which was shortened in response to various doses of Peiyuan Jieyu formula.Furthermore,formula administration significantly reduced serum TNF-a levels and hippocampal IDO mRNA expression.The high formula dose also reduced IFN-γand IBA-1 levels,the latter was also decreased in response to the medium formula dose.However,the low formula dose reduced serum KYN level and KYN/tryptophan(TRP)and KYN/KA ratios.Conclusion:The Peiyuan Jieyu formula holds immense potential in treating depression in a mouse model,potentially inhibiting inflammation and improving TRP-KYN metabolic disorders.展开更多
Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent years.Herein,taking advantage of the“lignin f...Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent years.Herein,taking advantage of the“lignin first”strategy,corn straw is converted to valuable chemicals including lignin monomers,furfural and 5-methoxymethylfurfural via a two steps process.The key of this research lies in the development of a green and low-cost catalytic process utilizing magnetic Raney Ni catalyst and high boiling point ethylene glycol.The utilization of neat ethylene glycol as the sole slovent under atmospheric conditions obviates the need for additional additives,thereby facilitating the entire process to be conducted in glass flasks and rendering it highly convenient for scaling up.In the initial step,depolymerization of corn straw lignin resulted in a monomer yield of 18.1 wt%.Subsequently,in a dimethyl carbonate system,the carbohydrate component underwent complete conversion in a one-pot process,yielding furfural and 5-methoxymethylfurfural as the primary products with an impressive yield of 47.7%.展开更多
The authors regret to report some missing information in the synthetic reagents and associated changes of the paper.On page 511,the author information reads:“5.0 mmol of citric acid(C_(6)H_(8)O_(7)),5.0 mmol of ferri...The authors regret to report some missing information in the synthetic reagents and associated changes of the paper.On page 511,the author information reads:“5.0 mmol of citric acid(C_(6)H_(8)O_(7)),5.0 mmol of ferric chloride hexahydrate(FeCl_(3)·6H_(2)O),and 10.0 mmol of o-phenylenediamine(C_(6)H_(8)N_(2))were combined with 40 mL of deionized water and magnetically stirred until fully dissolved.”展开更多
Micro-nano Earth Observation Satellite(MEOS)constellation has the advantages of low construction cost,short revisit cycle,and high functional density,which is considered a promising solution for serving rapidly growin...Micro-nano Earth Observation Satellite(MEOS)constellation has the advantages of low construction cost,short revisit cycle,and high functional density,which is considered a promising solution for serving rapidly growing observation demands.The observation Scheduling Problem in the MEOS constellation(MEOSSP)is a challenging issue due to the large number of satellites and tasks,as well as complex observation constraints.To address the large-scale and complicated MEOSSP,we develop a Two-Stage Scheduling Algorithm based on the Pointer Network with Attention mechanism(TSSA-PNA).In TSSA-PNA,the MEOS observation scheduling is decomposed into a task allocation stage and a single-MEOS scheduling stage.In the task allocation stage,an adaptive task allocation algorithm with four problem-specific allocation operators is proposed to reallocate the unscheduled tasks to new MEOSs.Regarding the single-MEOS scheduling stage,we design a pointer network based on the encoder-decoder architecture to learn the optimal singleMEOS scheduling solution and introduce the attention mechanism into the encoder to improve the learning efficiency.The Pointer Network with Attention mechanism(PNA)can generate the single-MEOS scheduling solution quickly in an end-to-end manner.These two decomposed stages are performed iteratively to search for the solution with high profit.A greedy local search algorithm is developed to improve the profits further.The performance of the PNA and TSSA-PNA on singleMEOS and multi-MEOS scheduling problems are evaluated in the experiments.The experimental results demonstrate that PNA can obtain the approximate solution for the single-MEOS scheduling problem in a short time.Besides,the TSSA-PNA can achieve higher observation profits than the existing scheduling algorithms within the acceptable computational time for the large-scale MEOS scheduling problem.展开更多
Lithium-selenium(Li-Se)batteries have attracted increasing attention as one of the next-generation battery systems due to much higher electronic conductivity and comparable volumetric capacity of Se compared to the po...Lithium-selenium(Li-Se)batteries have attracted increasing attention as one of the next-generation battery systems due to much higher electronic conductivity and comparable volumetric capacity of Se compared to the popular sulfur cathode.However,its practical application still faces great challenges,especially the rapid capacity decay triggered by the loss of active Se species.A comprehensive review to uncover the in-depth failure mechanism and provide targeted solutions to promote the stable operation of Li-Se batteries is urgently needed.This review systematically summarizes the strategies in the new perspective,focusing on the optimization of Se utilization in Li-Se batteries by keeping a high Se maintenance in the cathode and accelerating the electrochemical kinetics of lithium polyselenides(LiPSe)conversion.On the basis of stru ctural design and Li_(2)Se active material introduction to accommodate volume expansion,blocking s huttle transport of LiPSe by physical/chemical adsorption,bonding Se with polymers or cathode electrolyte interphase(CEI)construction,and catalytic design to accelerate the conversion of LiPSe,different strategies for improving the utilization of Se have been evaluated and discussed.To address the inevitable loss of Se,prospects on inactive Se reactivation and Li protection are detailedly proposed and analyzed referring to the chemistry and corrosion science.Additionally,the perspectives on the future design and comprehensive parameter evaluations for the optimization of Li-Se batteries are recommended.This review comprehensively explains the causes and solutions of capacity fading and provides potential efforts for lifespan expansion of batteries,shedding light on the future development of Li-Se batteries.展开更多
In the context of power generation companies, vast amounts of specialized data and expert knowledge have been accumulated. However, challenges such as data silos and fragmented knowledge hinder the effective utilizati...In the context of power generation companies, vast amounts of specialized data and expert knowledge have been accumulated. However, challenges such as data silos and fragmented knowledge hinder the effective utilization of this information. This study proposes a novel framework for intelligent Question-and-Answer (Q&A) systems based on Retrieval-Augmented Generation (RAG) to address these issues. The system efficiently acquires domain-specific knowledge by leveraging external databases, including Relational Databases (RDBs) and graph databases, without additional fine-tuning for Large Language Models (LLMs). Crucially, the framework integrates a Dynamic Knowledge Base Updating Mechanism (DKBUM) and a Weighted Context-Aware Similarity (WCAS) method to enhance retrieval accuracy and mitigate inherent limitations of LLMs, such as hallucinations and lack of specialization. Additionally, the proposed DKBUM dynamically adjusts knowledge weights within the database, ensuring that the most recent and relevant information is utilized, while WCAS refines the alignment between queries and knowledge items by enhanced context understanding. Experimental validation demonstrates that the system can generate timely, accurate, and context-sensitive responses, making it a robust solution for managing complex business logic in specialized industries.展开更多
The hybrid CO_(2) thermal technique has achieved considerable success globally in extracting residual heavy oil from reserves following a long-term steam stimulation process.Using microscopic visualization experiments...The hybrid CO_(2) thermal technique has achieved considerable success globally in extracting residual heavy oil from reserves following a long-term steam stimulation process.Using microscopic visualization experiments and molecular dynamics(MD)simulations,this study investigates the microscopic enhanced oil recovery(EOR)mechanisms underlying residual oil removal using hybrid CO_(2) thermal systems.Based on the experimental models for the occurrence of heavy oil,this study evaluates the performance of hybrid CO_(2) thermal systems under various conditions using MD simulations.The results demonstrate that introducing CO_(2) molecules into heavy oil can effectively penetrate and decompose dense aggregates that are originally formed on hydrophobic surfaces.A stable miscible hybrid CO_(2) thermal system,with a high effective distribution ratio of CO_(2),proficiently reduces the interaction energies between heavy oil and rock surfaces,as well as within heavy oil.A visualization analysis of the interactions reveals that strong van der Waals(vdW)attractions occur between CO_(2) and heavy oil molecules,effectively promoting the decomposition and swelling of heavy oil.This unlocks the residual oil on the hydrophobic surfaces.Considering the impacts of temperature and CO_(2) concentration,an optimal gas-to-steam injection ratio(here,the CO_(2):steam ratio)ranging between 1:6 and 1:9 is recommended.This study examines the microscopic mechanisms underlying the hybrid CO_(2) thermal technique at a molecular scale,providing a significant theoretical guide for its expanded application in EOR.展开更多
Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs...Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs) nanozyme is developed to target ROS, thereby reducing oxidative damage and improving the absorption and transfer of Cd ions in wheat. Notably, Fe-CQDs exhibit multi-enzyme activities mimicking peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), enabling effective neutralization of active species such as hydroxyl radicals (•OH), hydrogen peroxide (H_(2)O_(2)), and superoxide anions (O_(2)•^(-)). Importantly, root application of 10 mg L^(-1) Fe-CQDs alleviates Cd stress and promotes wheat growth in both hydroponic and soil cultures. Specifically, the levels of O_(2)•^(-), H_(2)O_(2), and malondialdehyde (MDA) in leaf tissues decrease, whereas the non-enzyme antioxidant, reduced glutathione (GSH), increases. Cell wall thickness in the Fe-CQDs-treated group is reduced by 42.4% compared with the Cd group. Moreover, Fe-CQDs enhance the expression of genes related to antioxidants, stress resistance, Cd detoxification, and nutrient transport. Transcriptomic and metabolomic analyses show that Fe-CQDs stimulate the production of flavonoids and regulate the activity of metal transporter genes (YSL, ABC, ZIP) to maintain ROS homeostasis. These findings highlight the potential of Fe-CQDs nanozyme platforms in mitigating oxidative damage and enhancing crop growth, offering new insights into the application of nanobiotechnology in agriculture.展开更多
Objective Emerging evidence suggests that exposure to ultrafine particulate matter(UPM,aerodynamic diameter<0.1μm)is associated with adverse cardiovascular events.Previous studies have found that Shenlian(SL)extra...Objective Emerging evidence suggests that exposure to ultrafine particulate matter(UPM,aerodynamic diameter<0.1μm)is associated with adverse cardiovascular events.Previous studies have found that Shenlian(SL)extract possesses anti-inflammatory and antiapoptotic properties and has a promising protective effect at all stages of the atherosclerotic disease process.In this study,we aimed to investigated whether SL improves UPM-aggravated myocardial ischemic injury by inhibiting inflammation and cell apoptosis.Methods We established a mouse model of MI+UPM.Echocardiographic measurement,measurement of myocardialinfarct size,biochemical analysis,enzyme-linked immunosorbent assay(ELISA),histopathological analysis,Transferase dUTP Nick End Labeling(TUNEL),Western blotting(WB),Polymerase Chain Reaction(PCR)and so on were used to explore the anti-inflammatory and antiapoptotic effects of SL in vivo and in vitro.Results SL treatment can attenuate UPM-induced cardiac dysfunction by improving left ventricular ejection fraction,fractional shortening,and decreasing cardiac infarction area.SL significantly reduced the levels of myocardial enzymes and attenuated UPM-induced morphological alterations.Moreover,SL significantly reduced expression levels of the inflammatory cytokines IL-6,TNF-α,and MCP-1.UPM further increased the infiltration of macrophages in myocardial tissue,whereas SL intervention reversed this phenomenon.UPM also triggered myocardial apoptosis,which was markedly attenuated by SL treatment.The results of in vitro experiments revealed that SL prevented cell damage caused by exposure to UPM combined with hypoxia by reducing the expression of the inflammatory factor NF-κB and inhibiting apoptosis in H9c2 cells.Conclusion Overall,both in vivo and in vitro experiments demonstrated that SL attenuated UPMaggravated myocardial ischemic injury by inhibiting inflammation and cell apoptosis.The mechanisms were related to the downregulation of macrophages infiltrating heart tissues.展开更多
The Belt and Road global navigation satellite system(B&R GNSS)network is the first large-scale deployment of Chinese GNSS equipment in a seismic system.Prior to this,there have been few systematic assessments of t...The Belt and Road global navigation satellite system(B&R GNSS)network is the first large-scale deployment of Chinese GNSS equipment in a seismic system.Prior to this,there have been few systematic assessments of the data quality of Chinese GNSS equipment.In this study,data from four representative GNSS sites in different regions of China were analyzed using the G-Nut/Anubis software package.Four main indicators(data integrity rate,data validity ratio,multi-path error,and cycle slip ratio)used to systematically analyze data quality,while evaluating the seismic monitoring capabilities of the network based on earthquake magnitudes estimated from high-frequency GNSS data are evaluated by estimating magnitude based on highfrequency GNSS data.The results indicate that the quality of the data produced by the three types of Chinese receivers used in the network meets the needs of earthquake monitoring and the new seismic industry standards,which provide a reference for the selection of equipment for future new projects.After the B&R GNSS network was established,the seismic monitoring capability for earthquakes with magnitudes greater than M_(W)6.5 in most parts of the Sichuan-Yunnan region improved by approximately 20%.In key areas such as the Sichuan-Yunnan Rhomboid Block,the monitoring capability increased by more than 25%,which has greatly improved the effectiveness of regional comprehensive earthquake management.展开更多
目的:通过SEDline镇静监测下右美托咪定在胸腔镜肺叶切除术病人中的应用,以期为麻醉选择更为安全有效的方法。方法:选择ASAⅠ或Ⅱ级的胸腔镜肺叶切除术病人20例,采用随机数字表法分为右美托咪定组(D组)和对照组(N组),每组10例。记录2组...目的:通过SEDline镇静监测下右美托咪定在胸腔镜肺叶切除术病人中的应用,以期为麻醉选择更为安全有效的方法。方法:选择ASAⅠ或Ⅱ级的胸腔镜肺叶切除术病人20例,采用随机数字表法分为右美托咪定组(D组)和对照组(N组),每组10例。记录2组病人麻醉诱导前(T_(0))、切皮时(T_(1))、单肺通气30 min(T_(2))、术毕即刻(T_(3))的平均动脉压(MAP)、心率(HR)、血氧饱和度(SPO 2)、病人状态指数(PSI);记录2组病人术中瑞芬太尼和丙泊酚的用量;以及术后6 h、24 h VAS评分和Ramsay评分。结果:2组病人在T_(1)、T_(2)、T_(3)时间点MAP均降低(P<0.01),HR减慢(P<0.01),PSI均降低(P<0.01)。其中,N组在T_(2)时间点MAP降低较D组更明显(P<0.01),D组在T_(1)时间点HR低于N组(P<0.01),在T_(2)时间点PSI低于N组(P<0.01);术中瑞芬太尼和丙泊酚的用量D组均明显低于N组;在术后6 h VAS评分D组低于N组(P<0.01),术后6 h Ramsay评分D组高于N组(P<0.01)。结论:胸腔镜肺叶切除术病人行右美托咪定联合SEDline镇静监测,术中病人麻醉状态得以控制,血流动力学更加平稳,术后镇静镇痛更加完善,无术后躁动,能对病人的快速康复起到一定的积极作用。展开更多
AIM: To test the hypothesis to block VEGF expression of SMMC-7721 hepatoma cells may inhibit tumor growth using the rat hepatoma model. METHODS: Amplify the 200 VEGF cDNA fragment and insert it into human U6 gene cass...AIM: To test the hypothesis to block VEGF expression of SMMC-7721 hepatoma cells may inhibit tumor growth using the rat hepatoma model. METHODS: Amplify the 200 VEGF cDNA fragment and insert it into human U6 gene cassette in the reverse orientation transcribing small antisense RNA which could specifically interact with VEGF165, and VEGF121 mRNA. Construct the retroviral vector containing this antisense VEGF U6 cassette and package the replication-deficient recombinant retrovirus. SMMC-7721 cells were transduced with these virus and positive clones were selected with G418. PCR and Southern blot analysis were performed to determine if U6 cassette integrated into the genomic DNA of positive clone. Transfected tumor cells were evaluated for RNA expression by ribonuclease protection assays. The VEGF protein in the supernatant of parental tumor cells and genetically modified tumor cells was determined with ELISA. In vitro and in vivo growth properties of antisense VEGF cell clone in nude mice were analyzed. RESULTS: Restriction enzyme digestion and PCR sequencing verified that the antisense VEGF RNA retroviral vector was successfully constructed.After G418 selection, resistant SMMC-7721 cell clone was picked up. PCR and Southern blot analysis suggested that U6 cassette was integrated into the cell genomic DNA. Stable SMMC-7721 cell clone transduced with U6 antisense RNA cassette could express 200 bp small antisense VEGF RNA and secrete reduced levels of VEGF in culture condition. Production of VEGF by antisense transgene-expressing cells was 65+/-10 ng/L per 10(6) cells, 42045 ng/L per 10(6) cells in sense group and 485+/-30 ng/L per 10(6) cells in the negative control group, (P【 0.05). The antisense-VEGF cell clone appeared phenotypically indistinguishable from SMMC-7721 cells and SMMC-7721 cells transfected sense VEGF. The growth rate of the antisense-VEGF cell clone was the same as the control cells. When S.C. was implanted into nude mice, growth of antisense-VEGF cell lines was greatly inhibited compared with control cells. CONCLUSION: Expression of antisense VEGF RNA in SMMC-7721 cells could decrease the tumorigenicity, and antisense-VEGF gene therapy may be an adjuvant treatment for hepatoma.展开更多
Background:Flammulina velutipes stem waste(FVS)is the by-product of mushroom industry.The objectives of this study were to determine the available energy and amino acid digestibility of FVS fed to pigs,and to evaluate...Background:Flammulina velutipes stem waste(FVS)is the by-product of mushroom industry.The objectives of this study were to determine the available energy and amino acid digestibility of FVS fed to pigs,and to evaluate the effects of dietary FVS inclusion on growth performance,biochemical profile of serum,fecal short chain fatty acid(SCFA)concentration,carcass traits,meat quality,intestinal morphology and microflora of pigs.In Exp.1,twelve crossbred barrows with initial body weight(IBW)of 37.48±4.31 kg were randomly allotted to 2 dietary treatments,including a corn basal diet and an experimental diet containing 24.35%FVS.In Exp.2,twelve barrows fitted with an ileal T-cannula(IBW:32.56±1.67 kg)were randomly allotted to 2 dietary treatments,which included a N-free diet and an experimental diet containing 40.0%FVS.In Exp.3,ninety growing pigs(IBW:63.98±6.89 kg)were allotted to 1 of 3 treatment diets for 63 d,including a basal diet and 2 experimental diets with 2.5%and 5%FVS,respectively.Results:The digestible energy(DE)and metabolizable energy(ME)of FVS were 4.58 and 4.06 MJ/kg on dry matter basis,respectively,and the standardized ileal digestibility(SID)of indispensable AAs ranged from 17.50%to 59.47%.Pigs fed diets with 2.5%FVS showed no difference on average daily gain(ADG)and gain to feed ratio(G/F).Although dietary 5%FVS inclusion impaired apparent total tract digestibility(ATTD)of organic matter and gross energy,it elevated the SCFA concentration(P≤0.04)in gut and antioxidant capacity in serum.In addition,dietary FVS inclusion depressed the backfat thickness(P=0.03)in pigs.The longissimus dorsi muscle of pigs fed FVS revealed higher n-3 polyunsaturated fatty acid concentration and optimized fatty acid composition.Dietary 2.5%FVS inclusion also improved the intestinal development and health by increasing the villius height to crypt depth ratio(V/C)in jejunum(P<0.01),and promoting microbial diversity and beneficial microbiota proliferation.Conclusions:It is feasible to include moderate content of FVS as an unconventional fiber ingredient in diet of growingfinishing pigs.展开更多
Edible fungi are large fungi with high added value that can be utilized as resources.They are rich in high-quality protein,carbohydrate,various vitamins,mineral elements and other nutrients,and are characterized by hi...Edible fungi are large fungi with high added value that can be utilized as resources.They are rich in high-quality protein,carbohydrate,various vitamins,mineral elements and other nutrients,and are characterized by high protein,low sugar,low fat and low cholesterol.In addition,edible fungi contain a variety of bioactive substances,such as polysaccharides,dietary fiber,steroids,polyphenols,and most of these compounds have antioxidant,anti-tumor and other physiological functions.This review comprehensively discusses the bioactive components and functional characteristics of edible fungi(such as antioxidant,anti-aging,hypolipidemic activities,etc.).Then the recent developments and prospect in the high-valued utilization of edible fungi are discussed and summarized.The objective of this review is to improve the understanding of health-promoting properties of edible fungi,and provide reference for the industrial production of edible fungi-based health products.展开更多
OBJECTIVE: To investigate the effective ingredients of Danshen(Radix Salviae Miltiorrhizae) and Honghua(Flos Carthami)(Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition...OBJECTIVE: To investigate the effective ingredients of Danshen(Radix Salviae Miltiorrhizae) and Honghua(Flos Carthami)(Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.METHODS: Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium(MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group,positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein(LDH), total superoxide dismutase(T-SOD), malondialdehyde(MDA), 6-keto prostaglandin F_(la)(6-keto-PGF_(1 a)), thromboxane B_2(TXB_2),mitochondrial membrane potential(MMP), free calcium ions concentration([Ca^(2 +)]i) and early onsetcell apoptosis.RESULTS: Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB2, improve level of 6-keto-PGF1 aand MMP, and prevent the early onset cell apoptosis.CONCLUSION: The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.展开更多
Backfill mining technology is the practice of returning waste materials underground for both disposal and geotechnical stability,however,a challenge with current technologies is that they commonly require cement-based...Backfill mining technology is the practice of returning waste materials underground for both disposal and geotechnical stability,however,a challenge with current technologies is that they commonly require cement-based binders which have a relatively high environmental impact.Finding alternatives to cement-based binders can improve environmental performance and this paper proposes microbial grouted backfill(MGB)as a potential solution.In this paper,the effects of the cementation solution concentration(CSC),volume ratio of bacterial solution to cementation solution(VRBC),particle sizes of the aggregates,and the number of grouting batches on the mechanical properties of MGB are studied.The experimental results show that MGB strength increased,up to a peak value,as CSC was increased,before decreasing as CSC was increased further.The results also show that MGB strength increased,up to a peak value,as VRBC decreased,before decreasing as the VRBC was decreased further.The peak strength was achieved at a CSC of 2 mol/L and a VRBC of 1:9.The strength of the MGB also increased as the number of grouting batches increased.Graded MGB samples showed the highest UCS,25.12 MPa,at particle sizes of 0.2 to 0.8 mm,while full(non-graded)MGB samples displayed mean UCS values ranging from1.56 MPa when the maximum particle size was 0.2 mm,up to 13 MPa when the maximum particle size was 1.2 mm.MGB samples are consolidated by the calcium carbonate that is precipitated during microbial metabolism,and the strength of MGB increases linearly as calcium carbonate content increases.The calcium carbonate minerals produced in MGB materials are primarily calcite,with secondary amounts of vaterite.展开更多
基金supported by the National Natural Science Foundation of China(81373584)。
文摘Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of depression by exposing mice that had previously received chronic stress to intraperitoneal LPS injections.The mice were divided into the following groups:control,model,fluoxetine,Tiansi Yin,Sini powder,and low-,medium-,and high-dose Peiyuan Jieyu formula groups.Forced swim and tail suspension tests were used to assess the efficacy of the depression(despair)model,and weight gain rates were also measured.Furthermore,serum levels of various depression and inflammation-associated molecules,including tumor necrosis factor-a(TNF-a),interferon-γ(IFN-γ),tryptophan,5-hydroxytryptamine,kynurenine(KYN),and kynurenic acid(KA)were assessed.Furthermore,the expression levels of ionic calcium-binding adaptor molecule-1(IBA-1)and indoleamine 2,3-dioxygenase(IDO)mRNA in hippocampal microglia were measured.Results:The model group displayed greater despair-associated immobility,which was shortened in response to various doses of Peiyuan Jieyu formula.Furthermore,formula administration significantly reduced serum TNF-a levels and hippocampal IDO mRNA expression.The high formula dose also reduced IFN-γand IBA-1 levels,the latter was also decreased in response to the medium formula dose.However,the low formula dose reduced serum KYN level and KYN/tryptophan(TRP)and KYN/KA ratios.Conclusion:The Peiyuan Jieyu formula holds immense potential in treating depression in a mouse model,potentially inhibiting inflammation and improving TRP-KYN metabolic disorders.
基金supported by the Fundamental Research Funds for the Central Universities(QNTD202302)National Natural Science Foundation of China(22378024)the Foreign expert program(G2022109001L).
文摘Developing a cost-effective and environmentally friendly process for the production of valuable chemicals from abundant herbal biomass receives great attentions in recent years.Herein,taking advantage of the“lignin first”strategy,corn straw is converted to valuable chemicals including lignin monomers,furfural and 5-methoxymethylfurfural via a two steps process.The key of this research lies in the development of a green and low-cost catalytic process utilizing magnetic Raney Ni catalyst and high boiling point ethylene glycol.The utilization of neat ethylene glycol as the sole slovent under atmospheric conditions obviates the need for additional additives,thereby facilitating the entire process to be conducted in glass flasks and rendering it highly convenient for scaling up.In the initial step,depolymerization of corn straw lignin resulted in a monomer yield of 18.1 wt%.Subsequently,in a dimethyl carbonate system,the carbohydrate component underwent complete conversion in a one-pot process,yielding furfural and 5-methoxymethylfurfural as the primary products with an impressive yield of 47.7%.
文摘The authors regret to report some missing information in the synthetic reagents and associated changes of the paper.On page 511,the author information reads:“5.0 mmol of citric acid(C_(6)H_(8)O_(7)),5.0 mmol of ferric chloride hexahydrate(FeCl_(3)·6H_(2)O),and 10.0 mmol of o-phenylenediamine(C_(6)H_(8)N_(2))were combined with 40 mL of deionized water and magnetically stirred until fully dissolved.”
基金supported by the National Natural Science Foundation of China(No.62101587)the National Funded Postdoctoral Researcher Program of China(No.GZC20233578)。
文摘Micro-nano Earth Observation Satellite(MEOS)constellation has the advantages of low construction cost,short revisit cycle,and high functional density,which is considered a promising solution for serving rapidly growing observation demands.The observation Scheduling Problem in the MEOS constellation(MEOSSP)is a challenging issue due to the large number of satellites and tasks,as well as complex observation constraints.To address the large-scale and complicated MEOSSP,we develop a Two-Stage Scheduling Algorithm based on the Pointer Network with Attention mechanism(TSSA-PNA).In TSSA-PNA,the MEOS observation scheduling is decomposed into a task allocation stage and a single-MEOS scheduling stage.In the task allocation stage,an adaptive task allocation algorithm with four problem-specific allocation operators is proposed to reallocate the unscheduled tasks to new MEOSs.Regarding the single-MEOS scheduling stage,we design a pointer network based on the encoder-decoder architecture to learn the optimal singleMEOS scheduling solution and introduce the attention mechanism into the encoder to improve the learning efficiency.The Pointer Network with Attention mechanism(PNA)can generate the single-MEOS scheduling solution quickly in an end-to-end manner.These two decomposed stages are performed iteratively to search for the solution with high profit.A greedy local search algorithm is developed to improve the profits further.The performance of the PNA and TSSA-PNA on singleMEOS and multi-MEOS scheduling problems are evaluated in the experiments.The experimental results demonstrate that PNA can obtain the approximate solution for the single-MEOS scheduling problem in a short time.Besides,the TSSA-PNA can achieve higher observation profits than the existing scheduling algorithms within the acceptable computational time for the large-scale MEOS scheduling problem.
基金supported by the China Scholarship Council(No.201809370046)a scholarship from the Laboratory of Inorganic Materials Chemistry,Universitéde Namur+5 种基金the National Key R&D Program of China(2016YFA0202602)the National Natural Science Foundation of China(No.U1663225,22293020,22293022 and 52103342)the Program of Introducing Talents of Discipline to Universities-Plan 111(Grant No.B20002)from the Ministry of Science and Technologythe Program for Changjiang Scholars and Innovative Research Team in University(IRT_15R52)of the Chinese Ministry of EducationBelgium-China Governmental Key Cooperation Program WBI-MOST(SUB/2021/IND493971/524448)the“Plan of relance”Wallonia Government(2310153-Bat Factory)。
文摘Lithium-selenium(Li-Se)batteries have attracted increasing attention as one of the next-generation battery systems due to much higher electronic conductivity and comparable volumetric capacity of Se compared to the popular sulfur cathode.However,its practical application still faces great challenges,especially the rapid capacity decay triggered by the loss of active Se species.A comprehensive review to uncover the in-depth failure mechanism and provide targeted solutions to promote the stable operation of Li-Se batteries is urgently needed.This review systematically summarizes the strategies in the new perspective,focusing on the optimization of Se utilization in Li-Se batteries by keeping a high Se maintenance in the cathode and accelerating the electrochemical kinetics of lithium polyselenides(LiPSe)conversion.On the basis of stru ctural design and Li_(2)Se active material introduction to accommodate volume expansion,blocking s huttle transport of LiPSe by physical/chemical adsorption,bonding Se with polymers or cathode electrolyte interphase(CEI)construction,and catalytic design to accelerate the conversion of LiPSe,different strategies for improving the utilization of Se have been evaluated and discussed.To address the inevitable loss of Se,prospects on inactive Se reactivation and Li protection are detailedly proposed and analyzed referring to the chemistry and corrosion science.Additionally,the perspectives on the future design and comprehensive parameter evaluations for the optimization of Li-Se batteries are recommended.This review comprehensively explains the causes and solutions of capacity fading and provides potential efforts for lifespan expansion of batteries,shedding light on the future development of Li-Se batteries.
文摘In the context of power generation companies, vast amounts of specialized data and expert knowledge have been accumulated. However, challenges such as data silos and fragmented knowledge hinder the effective utilization of this information. This study proposes a novel framework for intelligent Question-and-Answer (Q&A) systems based on Retrieval-Augmented Generation (RAG) to address these issues. The system efficiently acquires domain-specific knowledge by leveraging external databases, including Relational Databases (RDBs) and graph databases, without additional fine-tuning for Large Language Models (LLMs). Crucially, the framework integrates a Dynamic Knowledge Base Updating Mechanism (DKBUM) and a Weighted Context-Aware Similarity (WCAS) method to enhance retrieval accuracy and mitigate inherent limitations of LLMs, such as hallucinations and lack of specialization. Additionally, the proposed DKBUM dynamically adjusts knowledge weights within the database, ensuring that the most recent and relevant information is utilized, while WCAS refines the alignment between queries and knowledge items by enhanced context understanding. Experimental validation demonstrates that the system can generate timely, accurate, and context-sensitive responses, making it a robust solution for managing complex business logic in specialized industries.
基金financially supported by the National Natural Science Foundation of China(No.U20B6003)the China Scholarship Council(No.202306440015)a project of the China Petroleum&Chemical Corporation(No.P22174)。
文摘The hybrid CO_(2) thermal technique has achieved considerable success globally in extracting residual heavy oil from reserves following a long-term steam stimulation process.Using microscopic visualization experiments and molecular dynamics(MD)simulations,this study investigates the microscopic enhanced oil recovery(EOR)mechanisms underlying residual oil removal using hybrid CO_(2) thermal systems.Based on the experimental models for the occurrence of heavy oil,this study evaluates the performance of hybrid CO_(2) thermal systems under various conditions using MD simulations.The results demonstrate that introducing CO_(2) molecules into heavy oil can effectively penetrate and decompose dense aggregates that are originally formed on hydrophobic surfaces.A stable miscible hybrid CO_(2) thermal system,with a high effective distribution ratio of CO_(2),proficiently reduces the interaction energies between heavy oil and rock surfaces,as well as within heavy oil.A visualization analysis of the interactions reveals that strong van der Waals(vdW)attractions occur between CO_(2) and heavy oil molecules,effectively promoting the decomposition and swelling of heavy oil.This unlocks the residual oil on the hydrophobic surfaces.Considering the impacts of temperature and CO_(2) concentration,an optimal gas-to-steam injection ratio(here,the CO_(2):steam ratio)ranging between 1:6 and 1:9 is recommended.This study examines the microscopic mechanisms underlying the hybrid CO_(2) thermal technique at a molecular scale,providing a significant theoretical guide for its expanded application in EOR.
基金supported by the National Natural Science Foundation of China (3237152 and 22104102)Natural Science Foundation of Sichuan Province (2025ZNSFSC0155)+1 种基金Open Project Program (SKL-KF202416) of State Key Laboratory of Crop Gene Exploration and Utilization in Southwest ChinaTwo-Way Support Programs of Sichuan Agricultural University (P202105)
文摘Excessive accumulation of cadmium (Cd) impairs crop growth by inducing oxidative damage through the generation of reactive oxygen species (ROS). In this study, a biocompatible ferruginated carbon quantum dots (Fe-CQDs) nanozyme is developed to target ROS, thereby reducing oxidative damage and improving the absorption and transfer of Cd ions in wheat. Notably, Fe-CQDs exhibit multi-enzyme activities mimicking peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), enabling effective neutralization of active species such as hydroxyl radicals (•OH), hydrogen peroxide (H_(2)O_(2)), and superoxide anions (O_(2)•^(-)). Importantly, root application of 10 mg L^(-1) Fe-CQDs alleviates Cd stress and promotes wheat growth in both hydroponic and soil cultures. Specifically, the levels of O_(2)•^(-), H_(2)O_(2), and malondialdehyde (MDA) in leaf tissues decrease, whereas the non-enzyme antioxidant, reduced glutathione (GSH), increases. Cell wall thickness in the Fe-CQDs-treated group is reduced by 42.4% compared with the Cd group. Moreover, Fe-CQDs enhance the expression of genes related to antioxidants, stress resistance, Cd detoxification, and nutrient transport. Transcriptomic and metabolomic analyses show that Fe-CQDs stimulate the production of flavonoids and regulate the activity of metal transporter genes (YSL, ABC, ZIP) to maintain ROS homeostasis. These findings highlight the potential of Fe-CQDs nanozyme platforms in mitigating oxidative damage and enhancing crop growth, offering new insights into the application of nanobiotechnology in agriculture.
基金supported by CACMS Innovation Fund(No CI2021A04611,CI2021A05106)Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2021B015)+1 种基金Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2023E001TS01)Fundamental research funds for the central public welfare research institutes(L2022035).
文摘Objective Emerging evidence suggests that exposure to ultrafine particulate matter(UPM,aerodynamic diameter<0.1μm)is associated with adverse cardiovascular events.Previous studies have found that Shenlian(SL)extract possesses anti-inflammatory and antiapoptotic properties and has a promising protective effect at all stages of the atherosclerotic disease process.In this study,we aimed to investigated whether SL improves UPM-aggravated myocardial ischemic injury by inhibiting inflammation and cell apoptosis.Methods We established a mouse model of MI+UPM.Echocardiographic measurement,measurement of myocardialinfarct size,biochemical analysis,enzyme-linked immunosorbent assay(ELISA),histopathological analysis,Transferase dUTP Nick End Labeling(TUNEL),Western blotting(WB),Polymerase Chain Reaction(PCR)and so on were used to explore the anti-inflammatory and antiapoptotic effects of SL in vivo and in vitro.Results SL treatment can attenuate UPM-induced cardiac dysfunction by improving left ventricular ejection fraction,fractional shortening,and decreasing cardiac infarction area.SL significantly reduced the levels of myocardial enzymes and attenuated UPM-induced morphological alterations.Moreover,SL significantly reduced expression levels of the inflammatory cytokines IL-6,TNF-α,and MCP-1.UPM further increased the infiltration of macrophages in myocardial tissue,whereas SL intervention reversed this phenomenon.UPM also triggered myocardial apoptosis,which was markedly attenuated by SL treatment.The results of in vitro experiments revealed that SL prevented cell damage caused by exposure to UPM combined with hypoxia by reducing the expression of the inflammatory factor NF-κB and inhibiting apoptosis in H9c2 cells.Conclusion Overall,both in vivo and in vitro experiments demonstrated that SL attenuated UPMaggravated myocardial ischemic injury by inhibiting inflammation and cell apoptosis.The mechanisms were related to the downregulation of macrophages infiltrating heart tissues.
基金supported by grants from the National Natural Science Foundation of China(No.42004010)the B&R Seismic Monitoring Network Project of the China Earthquake Networks Center(No.5007).
文摘The Belt and Road global navigation satellite system(B&R GNSS)network is the first large-scale deployment of Chinese GNSS equipment in a seismic system.Prior to this,there have been few systematic assessments of the data quality of Chinese GNSS equipment.In this study,data from four representative GNSS sites in different regions of China were analyzed using the G-Nut/Anubis software package.Four main indicators(data integrity rate,data validity ratio,multi-path error,and cycle slip ratio)used to systematically analyze data quality,while evaluating the seismic monitoring capabilities of the network based on earthquake magnitudes estimated from high-frequency GNSS data are evaluated by estimating magnitude based on highfrequency GNSS data.The results indicate that the quality of the data produced by the three types of Chinese receivers used in the network meets the needs of earthquake monitoring and the new seismic industry standards,which provide a reference for the selection of equipment for future new projects.After the B&R GNSS network was established,the seismic monitoring capability for earthquakes with magnitudes greater than M_(W)6.5 in most parts of the Sichuan-Yunnan region improved by approximately 20%.In key areas such as the Sichuan-Yunnan Rhomboid Block,the monitoring capability increased by more than 25%,which has greatly improved the effectiveness of regional comprehensive earthquake management.
文摘目的:通过SEDline镇静监测下右美托咪定在胸腔镜肺叶切除术病人中的应用,以期为麻醉选择更为安全有效的方法。方法:选择ASAⅠ或Ⅱ级的胸腔镜肺叶切除术病人20例,采用随机数字表法分为右美托咪定组(D组)和对照组(N组),每组10例。记录2组病人麻醉诱导前(T_(0))、切皮时(T_(1))、单肺通气30 min(T_(2))、术毕即刻(T_(3))的平均动脉压(MAP)、心率(HR)、血氧饱和度(SPO 2)、病人状态指数(PSI);记录2组病人术中瑞芬太尼和丙泊酚的用量;以及术后6 h、24 h VAS评分和Ramsay评分。结果:2组病人在T_(1)、T_(2)、T_(3)时间点MAP均降低(P<0.01),HR减慢(P<0.01),PSI均降低(P<0.01)。其中,N组在T_(2)时间点MAP降低较D组更明显(P<0.01),D组在T_(1)时间点HR低于N组(P<0.01),在T_(2)时间点PSI低于N组(P<0.01);术中瑞芬太尼和丙泊酚的用量D组均明显低于N组;在术后6 h VAS评分D组低于N组(P<0.01),术后6 h Ramsay评分D组高于N组(P<0.01)。结论:胸腔镜肺叶切除术病人行右美托咪定联合SEDline镇静监测,术中病人麻醉状态得以控制,血流动力学更加平稳,术后镇静镇痛更加完善,无术后躁动,能对病人的快速康复起到一定的积极作用。
基金Project supported by National Natural Science Foundation of China,No.863 Z2001-04
文摘AIM: To test the hypothesis to block VEGF expression of SMMC-7721 hepatoma cells may inhibit tumor growth using the rat hepatoma model. METHODS: Amplify the 200 VEGF cDNA fragment and insert it into human U6 gene cassette in the reverse orientation transcribing small antisense RNA which could specifically interact with VEGF165, and VEGF121 mRNA. Construct the retroviral vector containing this antisense VEGF U6 cassette and package the replication-deficient recombinant retrovirus. SMMC-7721 cells were transduced with these virus and positive clones were selected with G418. PCR and Southern blot analysis were performed to determine if U6 cassette integrated into the genomic DNA of positive clone. Transfected tumor cells were evaluated for RNA expression by ribonuclease protection assays. The VEGF protein in the supernatant of parental tumor cells and genetically modified tumor cells was determined with ELISA. In vitro and in vivo growth properties of antisense VEGF cell clone in nude mice were analyzed. RESULTS: Restriction enzyme digestion and PCR sequencing verified that the antisense VEGF RNA retroviral vector was successfully constructed.After G418 selection, resistant SMMC-7721 cell clone was picked up. PCR and Southern blot analysis suggested that U6 cassette was integrated into the cell genomic DNA. Stable SMMC-7721 cell clone transduced with U6 antisense RNA cassette could express 200 bp small antisense VEGF RNA and secrete reduced levels of VEGF in culture condition. Production of VEGF by antisense transgene-expressing cells was 65+/-10 ng/L per 10(6) cells, 42045 ng/L per 10(6) cells in sense group and 485+/-30 ng/L per 10(6) cells in the negative control group, (P【 0.05). The antisense-VEGF cell clone appeared phenotypically indistinguishable from SMMC-7721 cells and SMMC-7721 cells transfected sense VEGF. The growth rate of the antisense-VEGF cell clone was the same as the control cells. When S.C. was implanted into nude mice, growth of antisense-VEGF cell lines was greatly inhibited compared with control cells. CONCLUSION: Expression of antisense VEGF RNA in SMMC-7721 cells could decrease the tumorigenicity, and antisense-VEGF gene therapy may be an adjuvant treatment for hepatoma.
基金This research was jointly supported by the Special Fund for Agro-scientific Research in the Public Interest(201503137)Program of Creation and Utilization of Germplasm of Mushroom Crop of“111”Project(D17014)+1 种基金National-level International Joint Research Centre(2017B01011)and the Changchun Science and Technology Project(15SS11).
文摘Background:Flammulina velutipes stem waste(FVS)is the by-product of mushroom industry.The objectives of this study were to determine the available energy and amino acid digestibility of FVS fed to pigs,and to evaluate the effects of dietary FVS inclusion on growth performance,biochemical profile of serum,fecal short chain fatty acid(SCFA)concentration,carcass traits,meat quality,intestinal morphology and microflora of pigs.In Exp.1,twelve crossbred barrows with initial body weight(IBW)of 37.48±4.31 kg were randomly allotted to 2 dietary treatments,including a corn basal diet and an experimental diet containing 24.35%FVS.In Exp.2,twelve barrows fitted with an ileal T-cannula(IBW:32.56±1.67 kg)were randomly allotted to 2 dietary treatments,which included a N-free diet and an experimental diet containing 40.0%FVS.In Exp.3,ninety growing pigs(IBW:63.98±6.89 kg)were allotted to 1 of 3 treatment diets for 63 d,including a basal diet and 2 experimental diets with 2.5%and 5%FVS,respectively.Results:The digestible energy(DE)and metabolizable energy(ME)of FVS were 4.58 and 4.06 MJ/kg on dry matter basis,respectively,and the standardized ileal digestibility(SID)of indispensable AAs ranged from 17.50%to 59.47%.Pigs fed diets with 2.5%FVS showed no difference on average daily gain(ADG)and gain to feed ratio(G/F).Although dietary 5%FVS inclusion impaired apparent total tract digestibility(ATTD)of organic matter and gross energy,it elevated the SCFA concentration(P≤0.04)in gut and antioxidant capacity in serum.In addition,dietary FVS inclusion depressed the backfat thickness(P=0.03)in pigs.The longissimus dorsi muscle of pigs fed FVS revealed higher n-3 polyunsaturated fatty acid concentration and optimized fatty acid composition.Dietary 2.5%FVS inclusion also improved the intestinal development and health by increasing the villius height to crypt depth ratio(V/C)in jejunum(P<0.01),and promoting microbial diversity and beneficial microbiota proliferation.Conclusions:It is feasible to include moderate content of FVS as an unconventional fiber ingredient in diet of growingfinishing pigs.
基金This review was financially supported by the Key Projects of the National Research and Development Program of China(Grant No.2018YFD0400204).
文摘Edible fungi are large fungi with high added value that can be utilized as resources.They are rich in high-quality protein,carbohydrate,various vitamins,mineral elements and other nutrients,and are characterized by high protein,low sugar,low fat and low cholesterol.In addition,edible fungi contain a variety of bioactive substances,such as polysaccharides,dietary fiber,steroids,polyphenols,and most of these compounds have antioxidant,anti-tumor and other physiological functions.This review comprehensively discusses the bioactive components and functional characteristics of edible fungi(such as antioxidant,anti-aging,hypolipidemic activities,etc.).Then the recent developments and prospect in the high-valued utilization of edible fungi are discussed and summarized.The objective of this review is to improve the understanding of health-promoting properties of edible fungi,and provide reference for the industrial production of edible fungi-based health products.
基金Supported by National Natural Science Foundation of China:Biological Foundation Research Related to Ischemic Stroke in Qi Deficiency and Blood Stasis Syndrome Based on Drug Metabolism and Biological Effects of Buyang Huanwu Decotion and Its Associated Prescriptions(No.81630105)Effect of Danhong Injection on Cerebral Ischemia Reperfusion Injury in Rats Mediated by Aldose Reductase(No.81673639)+3 种基金Zhejiang Provincial Natural Science Foundation of China:Mechanism of the Component Compatibility of Salvia Miltiorrhiza and Safflower against Cerebral Ischemic Injury Based on the Association of PK-PD(No.LZ17H270001)Zhejiang Provincial Program for the health leading talentsBasic public welfare research project of Zhejiang Province:Investigation and Application of Optimization of Chinese Traditional Medicine Extraction Process Based on Genetic Algorithm-neural Network Approach(No.LGN18A010001)Scientific Research Foundation of Zhejiang Chinese Medicine University:Mechanism of Danhong Injection against Cerebral Ischemic Injury Based on Pharmacokinetic Efficacy(PK-PD)Relationship(No.2017ZZ09)
文摘OBJECTIVE: To investigate the effective ingredients of Danshen(Radix Salviae Miltiorrhizae) and Honghua(Flos Carthami)(Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.METHODS: Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium(MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group,positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein(LDH), total superoxide dismutase(T-SOD), malondialdehyde(MDA), 6-keto prostaglandin F_(la)(6-keto-PGF_(1 a)), thromboxane B_2(TXB_2),mitochondrial membrane potential(MMP), free calcium ions concentration([Ca^(2 +)]i) and early onsetcell apoptosis.RESULTS: Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB2, improve level of 6-keto-PGF1 aand MMP, and prevent the early onset cell apoptosis.CONCLUSION: The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.
基金supported by the National Natural Science Foundation of China(Nos.5180430852034009)+3 种基金the China Postdoctoral Science Foundation(Nos.2020T1302692020M670689)the Yue Qi Young Scholar Project(No.2020QN03)the Postdoctoral Research Project of Hebei Province(No.B2020003029)。
文摘Backfill mining technology is the practice of returning waste materials underground for both disposal and geotechnical stability,however,a challenge with current technologies is that they commonly require cement-based binders which have a relatively high environmental impact.Finding alternatives to cement-based binders can improve environmental performance and this paper proposes microbial grouted backfill(MGB)as a potential solution.In this paper,the effects of the cementation solution concentration(CSC),volume ratio of bacterial solution to cementation solution(VRBC),particle sizes of the aggregates,and the number of grouting batches on the mechanical properties of MGB are studied.The experimental results show that MGB strength increased,up to a peak value,as CSC was increased,before decreasing as CSC was increased further.The results also show that MGB strength increased,up to a peak value,as VRBC decreased,before decreasing as the VRBC was decreased further.The peak strength was achieved at a CSC of 2 mol/L and a VRBC of 1:9.The strength of the MGB also increased as the number of grouting batches increased.Graded MGB samples showed the highest UCS,25.12 MPa,at particle sizes of 0.2 to 0.8 mm,while full(non-graded)MGB samples displayed mean UCS values ranging from1.56 MPa when the maximum particle size was 0.2 mm,up to 13 MPa when the maximum particle size was 1.2 mm.MGB samples are consolidated by the calcium carbonate that is precipitated during microbial metabolism,and the strength of MGB increases linearly as calcium carbonate content increases.The calcium carbonate minerals produced in MGB materials are primarily calcite,with secondary amounts of vaterite.
