Osteoporosis represents a prevalent and debilitating comorbidity in patients diagnosed with type 2 diabetes mellitus(T2DM),which is characterized by suppressed osteoblast function and disrupted bone microarchitecture....Osteoporosis represents a prevalent and debilitating comorbidity in patients diagnosed with type 2 diabetes mellitus(T2DM),which is characterized by suppressed osteoblast function and disrupted bone microarchitecture.In this study,we utilized male C57BL/6 J mice to investigate the role of SIRT3 in T2DM.Decreased SIRT3 expression and impaired mitochondrial quality control mechanism are observed in both in vitro and in vivo models of T2DM.Mechanistically,SIRT3 suppression results in hyperacetylation of FOXO3,hindering the activation of the PINK1/PRKN mediated mitophagy pathway and resulting in accumulation of dysfunctional mitochondria.Genetical overexpression or pharmacological activation of SIRT3 restores deacetylation status of FOXO3,thus facilitating mitophagy and ameliorating osteogenic impairment in T2DM.Collectively,our findings highlight the fundamental regulatory function of SIRT3 in mitochondrial quality control,crucial for maintaining bone homeostasis in T2DM.These insights not only enhance our understanding of the molecular mechanisms underlying diabetic osteoporosis but also identify SIRT3 as a promising therapeutic target for diabetic osteoporosis.展开更多
随着城市化进程的加快,废土管理问题日益严峻。本文提出了一种基于固废胶凝材料的土壤固化方法,利用矿渣、钢渣和脱硫石膏作为固化剂,通过混料设计研究了不同配合比对砂质粉土无侧限抗压强度(UCS)的影响,并利用人工神经网络(ANN)建立了...随着城市化进程的加快,废土管理问题日益严峻。本文提出了一种基于固废胶凝材料的土壤固化方法,利用矿渣、钢渣和脱硫石膏作为固化剂,通过混料设计研究了不同配合比对砂质粉土无侧限抗压强度(UCS)的影响,并利用人工神经网络(ANN)建立了固化土UCS预测模型。结果表明,固废胶凝材料显著提升了固化土的早期和后期UCS,尤其是钢渣含量不高于30%(质量分数)时,固化土UCS优于水泥固化土。X射线衍射(XRD)和扫描电子显微镜(SEM)分析表明,水化产物钙矾石(AFt)和水化硅酸钙(C-S-H)凝胶填充了土壤孔隙,提升了固化土的密实度和力学性能。基于ANN构建的7、28 d UCS预测模型,决定系数R^(2)均在0.95以上,表现出较高的预测精度。通过特征重要性分析,胶凝材料掺量和土壤含水率对固化土UCS的影响较为显著。研究结果为固废胶凝材料的配合比优化和工程应用提供了理论依据。展开更多
为了改善玉米淀粉的加工特性,提升产品品质,研究了黄原胶和3种不同链长的脂肪酸(肉豆蔻酸、棕榈酸和硬脂酸)的组合对玉米淀粉糊化性质、流变性质、凝胶性质、热性质和凝胶微观结构的影响。快速黏度分析结果表明,添加黄原胶-棕榈酸和黄原...为了改善玉米淀粉的加工特性,提升产品品质,研究了黄原胶和3种不同链长的脂肪酸(肉豆蔻酸、棕榈酸和硬脂酸)的组合对玉米淀粉糊化性质、流变性质、凝胶性质、热性质和凝胶微观结构的影响。快速黏度分析结果表明,添加黄原胶-棕榈酸和黄原胶-硬脂酸组合后玉米淀粉糊的峰值黏度和最终黏度分别增加了123~131 m Pa·s和64~94 m Pa·s,回生值降低了16~18 m Pa·s,而添加黄原胶-肉豆蔻酸组合的淀粉糊峰值黏度和最终黏度降低,回生值增加。静态流变结果表明,加入黄原胶和3种不同链长脂肪酸组合的玉米淀粉均表现出非牛顿剪切稀化行为(流动行为指数n<1)。动态流变结果显示,加入黄原胶-棕榈酸和黄原胶-硬脂酸组合后,玉米淀粉的储能模量G′和损耗模量G″增加,表明加入这两种组合可有效的改善玉米淀粉的黏弹性,而加入黄原胶-肉豆蔻酸组合的玉米淀粉的G′和G″值降低。添加黄原胶和3种不同链长脂肪酸组合后,玉米淀粉凝胶硬度降低了65~77g,差示扫描量热仪结果显示在89~110℃出现吸热峰,表明形成了直链淀粉-脂肪酸复合物;扫描电镜观察到淀粉凝胶网络结构断裂程度增加,呈现碎片化结构,表明加入黄原胶和脂肪酸的组合后,淀粉的凝胶结构被弱化。展开更多
基金supported by the National Key Research and Development Project (2021YFA1201404)National Natural Science Foundation of China Major Project (81991514)+6 种基金General Project (82272530, 82372459)Jiangsu Province Medical Innovation Center of Orthopedic Surgery (CXZX202214)Jiangsu Provincial Key Medical Center FoundationJiangsu Provincial Medical Outstanding Talent FoundationJiangsu Provincial Medical Youth Talent FoundationJiangsu Provincial Key Medical Talent Foundationthe Fundamental Research Funds for the Central Universities (14380493, 14380494)
文摘Osteoporosis represents a prevalent and debilitating comorbidity in patients diagnosed with type 2 diabetes mellitus(T2DM),which is characterized by suppressed osteoblast function and disrupted bone microarchitecture.In this study,we utilized male C57BL/6 J mice to investigate the role of SIRT3 in T2DM.Decreased SIRT3 expression and impaired mitochondrial quality control mechanism are observed in both in vitro and in vivo models of T2DM.Mechanistically,SIRT3 suppression results in hyperacetylation of FOXO3,hindering the activation of the PINK1/PRKN mediated mitophagy pathway and resulting in accumulation of dysfunctional mitochondria.Genetical overexpression or pharmacological activation of SIRT3 restores deacetylation status of FOXO3,thus facilitating mitophagy and ameliorating osteogenic impairment in T2DM.Collectively,our findings highlight the fundamental regulatory function of SIRT3 in mitochondrial quality control,crucial for maintaining bone homeostasis in T2DM.These insights not only enhance our understanding of the molecular mechanisms underlying diabetic osteoporosis but also identify SIRT3 as a promising therapeutic target for diabetic osteoporosis.
文摘随着城市化进程的加快,废土管理问题日益严峻。本文提出了一种基于固废胶凝材料的土壤固化方法,利用矿渣、钢渣和脱硫石膏作为固化剂,通过混料设计研究了不同配合比对砂质粉土无侧限抗压强度(UCS)的影响,并利用人工神经网络(ANN)建立了固化土UCS预测模型。结果表明,固废胶凝材料显著提升了固化土的早期和后期UCS,尤其是钢渣含量不高于30%(质量分数)时,固化土UCS优于水泥固化土。X射线衍射(XRD)和扫描电子显微镜(SEM)分析表明,水化产物钙矾石(AFt)和水化硅酸钙(C-S-H)凝胶填充了土壤孔隙,提升了固化土的密实度和力学性能。基于ANN构建的7、28 d UCS预测模型,决定系数R^(2)均在0.95以上,表现出较高的预测精度。通过特征重要性分析,胶凝材料掺量和土壤含水率对固化土UCS的影响较为显著。研究结果为固废胶凝材料的配合比优化和工程应用提供了理论依据。
文摘为了改善玉米淀粉的加工特性,提升产品品质,研究了黄原胶和3种不同链长的脂肪酸(肉豆蔻酸、棕榈酸和硬脂酸)的组合对玉米淀粉糊化性质、流变性质、凝胶性质、热性质和凝胶微观结构的影响。快速黏度分析结果表明,添加黄原胶-棕榈酸和黄原胶-硬脂酸组合后玉米淀粉糊的峰值黏度和最终黏度分别增加了123~131 m Pa·s和64~94 m Pa·s,回生值降低了16~18 m Pa·s,而添加黄原胶-肉豆蔻酸组合的淀粉糊峰值黏度和最终黏度降低,回生值增加。静态流变结果表明,加入黄原胶和3种不同链长脂肪酸组合的玉米淀粉均表现出非牛顿剪切稀化行为(流动行为指数n<1)。动态流变结果显示,加入黄原胶-棕榈酸和黄原胶-硬脂酸组合后,玉米淀粉的储能模量G′和损耗模量G″增加,表明加入这两种组合可有效的改善玉米淀粉的黏弹性,而加入黄原胶-肉豆蔻酸组合的玉米淀粉的G′和G″值降低。添加黄原胶和3种不同链长脂肪酸组合后,玉米淀粉凝胶硬度降低了65~77g,差示扫描量热仪结果显示在89~110℃出现吸热峰,表明形成了直链淀粉-脂肪酸复合物;扫描电镜观察到淀粉凝胶网络结构断裂程度增加,呈现碎片化结构,表明加入黄原胶和脂肪酸的组合后,淀粉的凝胶结构被弱化。
