For deep prospects in the foreland thrust belt,southern Junggar Basin,NW China,there are uncertainties in factors controlling the structural deformation,distribution of paleo-structures and detachment layers,and distr...For deep prospects in the foreland thrust belt,southern Junggar Basin,NW China,there are uncertainties in factors controlling the structural deformation,distribution of paleo-structures and detachment layers,and distribution of major hydrocarbon source rocks.Based on the latest 3D seismic,gravity-magnetic,and drilling data,together with the results of previous structural physical simulation and discrete element numerical simulation experiments,the spatial distribution of pre-existing paleo-structures and detachment layers in deep strata of southern Junggar Basin were systematically characterized,the structural deformation characteristics and formation mechanisms were analyzed,the distribution patterns of multiple hydrocarbon source rock suites were clarified,and hydrocarbon accumulation features in key zones were reassessed.The exploration targets in deep lower assemblages with possibility of breakthrough were expected.Key results are obtained in three aspects.First,structural deformation is controlled by two-stage paleo-structures and three detachment layers with distinct lateral variations:the Jurassic layer(moderate thickness,wide distribution),the Cretaceous layer(thickest but weak detachment),and the Paleogene layer(thin but long-distance lateral thrusting).Accordingly,a four-layer composite deformation sequence was identified,and the structural genetic model with paleo-bulge controlling zonation by segments laterally and multiple detachment layers controlling sequence vertically.Second,the Permian source rocks show a distribution pattern with narrow trough(west),multiple sags(central),and broad basin(east),which is depicted by combining high-precision gravity-magnetic data and time-frequency electromagnetic data for the first time,and the Jurassic source rocks feature thicker mudstones in the west and rich coals in the east according to the reassessment.Third,two petroleum systems and a four-layer composite hydrocarbon accumulation model are established depending on the structural deformation strength,trap effectiveness and source-trap configuration.The southern Junggar Basin is divided into three segments with ten zones,and a hierarchical exploration strategy is proposed for deep lower assemblages in this region,that is,focusing on five priority zones,expanding to three potential areas,and challenging two high-risk targets.展开更多
Objective:The asparagine-linked glycosylation 13 homolog(Alg13)has been identified as causative for congenital disorders of glycosylation type I with epilepsy.The aim of this study was to determine whether mice carryi...Objective:The asparagine-linked glycosylation 13 homolog(Alg13)has been identified as causative for congenital disorders of glycosylation type I with epilepsy.The aim of this study was to determine whether mice carrying a mutated version of Alg13 could be used as a model for epileptic encephalopathies or congenital disorders of glycosylation type I.Methods:A model of epileptic encephalopathy was established in C57BL/6 mice by introducing mutations in Alg13 via the clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(CRISPR/Cas9)system.All surgical procedures were approved by the Institutional Animal Care and Use Committee of Shanghai Jiao Tong University(A2016084)on October 8,2016.Results:Mice with 3 different mutations,Alg13^(-54nt),Alg13^(-5nt)and Alg13^(-4nt),all of which are located in Alg13 transcript variant 1,were created.The Alg13^(-5nt)mice exhibited spontaneous seizures similar to patients with Alg13 mutations,suggesting that they could be used as a model for epilepsy.Western blot analysis demonstrated that Alg13^(-5nt)mice had lower levels of Alg13 expression than wild-type mice.Video observations showed that two of the 17 Alg13^(-5nt)mice had stage 5 seizures involving jumping and falling,while 12 had stage 3 seizures with head nodding.Conclusion:The Alg13 mouse model provides an outstanding tool for studying epileptic encephalopathies and investigating different aspects of defects in glycosylation or other post-translational modification that cannot be assessed in patients or cell culture systems.展开更多
基金Supported by the Science and Technology Special Project of CNPC(2023YQX10111)Key Research and Development Special Project of Xinjiang Uygur Autonomous Region(2024B01015-3)。
文摘For deep prospects in the foreland thrust belt,southern Junggar Basin,NW China,there are uncertainties in factors controlling the structural deformation,distribution of paleo-structures and detachment layers,and distribution of major hydrocarbon source rocks.Based on the latest 3D seismic,gravity-magnetic,and drilling data,together with the results of previous structural physical simulation and discrete element numerical simulation experiments,the spatial distribution of pre-existing paleo-structures and detachment layers in deep strata of southern Junggar Basin were systematically characterized,the structural deformation characteristics and formation mechanisms were analyzed,the distribution patterns of multiple hydrocarbon source rock suites were clarified,and hydrocarbon accumulation features in key zones were reassessed.The exploration targets in deep lower assemblages with possibility of breakthrough were expected.Key results are obtained in three aspects.First,structural deformation is controlled by two-stage paleo-structures and three detachment layers with distinct lateral variations:the Jurassic layer(moderate thickness,wide distribution),the Cretaceous layer(thickest but weak detachment),and the Paleogene layer(thin but long-distance lateral thrusting).Accordingly,a four-layer composite deformation sequence was identified,and the structural genetic model with paleo-bulge controlling zonation by segments laterally and multiple detachment layers controlling sequence vertically.Second,the Permian source rocks show a distribution pattern with narrow trough(west),multiple sags(central),and broad basin(east),which is depicted by combining high-precision gravity-magnetic data and time-frequency electromagnetic data for the first time,and the Jurassic source rocks feature thicker mudstones in the west and rich coals in the east according to the reassessment.Third,two petroleum systems and a four-layer composite hydrocarbon accumulation model are established depending on the structural deformation strength,trap effectiveness and source-trap configuration.The southern Junggar Basin is divided into three segments with ten zones,and a hierarchical exploration strategy is proposed for deep lower assemblages in this region,that is,focusing on five priority zones,expanding to three potential areas,and challenging two high-risk targets.
基金This study was partially supported by the National Natural Science Foundation of China(Nos.81720108017,81370675 and 81421061)Scientific Research Program of Ningxia Medical University,China(No.2002080101).
文摘Objective:The asparagine-linked glycosylation 13 homolog(Alg13)has been identified as causative for congenital disorders of glycosylation type I with epilepsy.The aim of this study was to determine whether mice carrying a mutated version of Alg13 could be used as a model for epileptic encephalopathies or congenital disorders of glycosylation type I.Methods:A model of epileptic encephalopathy was established in C57BL/6 mice by introducing mutations in Alg13 via the clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(CRISPR/Cas9)system.All surgical procedures were approved by the Institutional Animal Care and Use Committee of Shanghai Jiao Tong University(A2016084)on October 8,2016.Results:Mice with 3 different mutations,Alg13^(-54nt),Alg13^(-5nt)and Alg13^(-4nt),all of which are located in Alg13 transcript variant 1,were created.The Alg13^(-5nt)mice exhibited spontaneous seizures similar to patients with Alg13 mutations,suggesting that they could be used as a model for epilepsy.Western blot analysis demonstrated that Alg13^(-5nt)mice had lower levels of Alg13 expression than wild-type mice.Video observations showed that two of the 17 Alg13^(-5nt)mice had stage 5 seizures involving jumping and falling,while 12 had stage 3 seizures with head nodding.Conclusion:The Alg13 mouse model provides an outstanding tool for studying epileptic encephalopathies and investigating different aspects of defects in glycosylation or other post-translational modification that cannot be assessed in patients or cell culture systems.
